IL1 alpha (IL1A) (BC013142) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hematopoietin-1; IL-1A; IL1; IL1-ALPHA; IL1A (IL1F1); IL1F1; interleukin 1, alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC013142 edited GAAGTCAAGATGGCCAAAGTTCCAGACATGTTTGAAGACCTGAAGAACTGTTACAGTGAA AATGAAGAAGACAGTTCCTCCATTGATCATCTGTCTCTGAATCAGAAATCCTTCTATCAT GTAAGCTATGGCCCACTCCATGAAGGCTGCATGGATCAATCTGTGTCTCTGAGTATCTCT GAAACCTCTAAAACATCCAAGCTTACCTTCAAGGAGAGCATGGTGGTAGTAGCAACCAAC GGGAAGGTTCTGAAGAAGAGACGGTTGAGTTTAAGCCAATCCATCACTGATGATGACCTG GAGGCCATCGCCAATGACTCAGAGGAAGAAATCATCAAGCCTAGGTCAGCACCTTTTAGC TTCCTGAGCAATGTGAAATACAACTTTATGAGGATCATCAAATACGAATTCATCCTGAAT GACGCCCTCAATCAAAGTATAATTCGAGCCAATGATCAGTACCTCACGGCTGCTGCATTA CATAATCTGGATGAAGCAGTGAAATTTGACATGGGTGCTTATAAGTCATCAAAGGATGAT GCTAAAATTACCGTGATTCTAAGAATCTCAAAAACTCAATTGTATGTGACTGCCCAAGAT GAAGACCAACCAGTGCTGCTGAAGGAGATGCCTGAGATACCCAAAACCATCACAGGTAGT GAGACCAACCTCCTCTTCTTCTGGGAAACTCACGGCACTAAGAACTATTTCACATCAGTT GCCCATCCAAACTTGTTTATTGCCACAAAGCAAGACTACTGGGTGTGCTTGGCAGGGGGG CCACCCTCTATCACTGACTTTCAGATACTGGAAAACCAGGCGTAGGTCTGGAGTCTCACT TGTCTCACTTGTGCAGTGTTGACAGTTCATATGTACCATGTACATGAAGAAGCTAAATCC TTTACTGTTAGTCATTTGCTGAGCATGTACTGAGCCTTGTAATTCTAAATGAATGTTTAC ACTCTTTGTAAGAGTGGAACCAACACTAACATATAATGTTGTTATTTAAAGAACACCCTA TATTTTGCATAGTACCAATCATTTTAATTATTATTCTTCATAACAATTTTAGGAGGACCA GAGCTACTGACTATGGCTACCAAAAAGACTCTACCCATATTACAGATGGGCAAATTAAGG CATAAGAAAACTAAGAAATATGCACAATAGCAGTTGAAACAAGAAGCCACAGACCTAGGA TTTCATGATTTCATTTCAACTGTTTGCCTTCTACTTTTAAGTTGCTGATGAACTCTTAAT CAAATAGCATAAGTTTCTGGGACCTCAGTTTTATCATTTTCAAAATGGAGGGAATAATAC CTAAGCCTTCCTGCCGCAACAGTTTTTTATGCTAATCAGGGAGGTCATTTTGGTAAAATA CTTCTTGAAGCCGAGCCTCAAGATGAAGGCAAAGCACGAAATGTTATTTTTTAATTATTA TTTATATATGTATTTATAAATATATTTAAGATAATTATAATATACTATATTTATGGGAAC CCCTTCATCCTCTGAGTGTGACCAGGCATCCTCCACAATAGCAGACAGTGTTTTCTGGGA TAAGTAAGTTTGATTTCATTAATACAGGGCATTTTGGTCCAAGTTGTGCTTATCCCATAG CCAGGAAACTCTGCATTCTAGTACTTGGGAGACCTGTAATCATATAATAAATGTACATTA ATTACCTTGAGCCAGTAATTGGTCCGATCTTTGACTCTTTTGCCATTAAACTTACCTGGG CATTCTTGTTTCAATTCCACCTGCAATCAAGTCCTACAAGCTAAAATTAGATGAACTCAA CTTTGACAACCATGAGACCACTGTTATCAAAACTTTCTTTTCTGGAATGTAATCAATGTT TCTTCTAGGTTCTAAAAATTGTGATCAGACCATAATGTTACATTATTATCAACAATAGTG ATTGATAGAGTGTTATCAGTCATAACTAAATAAAGCTTGCAACAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC013142 unedited CGCGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGCATTCAAATCTGGTACCGGTC CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGTCATTTCATTGGCGTTTGAGTCAG CAAAGAAGTCAAGATGGCCAAAGTTCCAGACATGTTTGAAGACCTGAAGAACTGTTACAG TGAAAATGAAGAAGACAGTTCCTCCATTGATCATCTGTCTCTGAATCAGAAATCCTTCTA TCATGTAAGCTATGGCCCACTCCATGAAGGCTGCATGGATCAATCTGTGTCTCTGAGTAT CTCTGAAACCTCTAAAACATCCAAGCTTACCTTCAAGGAGAGCATGGTGGTAGTAGCAAC CAACGGGAAGGTTCTGAAGAAGAGACGGTTGAGTTTAAGCCAATCCATCACTGATGATGA CCTGGAGGCCATCGCCAATGACTCAGAGGAAGAAATCATCAAGCCTAGGTCAGCACCTTT TAGCTTCCTGAGCAATGTGAAATACAACTTTATGAGGATCATCAAATACGAATTCATCCT GAATGACGCCCTCCATCAAAGTATAATTCGAGCCAATGATCAGTACCTCACGGCTGCTGC ATTACATAATCTGGATGAAGCAGTGAAATTTGACATGGGTGCTTATAAGTCATCAAAGGA TGATGCTAAAATTACCGTGATTCTAAGAATCTCAAAAACTCAATTGTATGTGACTGCCCC AGATGAAGACCAACCAGTGCTGCTGAAGGAGATGCCTGAGATACCCAAACCCTCACAAGT AGTGGAGACCAACCTTCTCTTCTTCTGGGAAACTCACGGCACTAAAAACTATTCACATCC GTTGCCCATCCCAAATTGGTTAATTGCCACAACGCCAAGACTACTTGGGTGGGCTTTGA
Restriction Sites	Please inquire
ACCN	BC013142
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	no
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013142.2, AAH13142.1
RefSeq Size	1978 bp
Locus ID	3552
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus
Gene Summary	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.