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CHRNA5 (NM_000745) Human Untagged Clone
CAT＃: SC122164
CHRNA5 (untagged)-Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5)
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LNCR2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC122164 sequence for NM_000745 edited (data generated by NextGen Sequencing)
ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC GCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCT TCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGA TGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGACTTGCAATA TCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTCTGGTTGAAA CAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGAATAAAAGTT ATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT GGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACCTGG ACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTTGAC CTTCAGAACTGTTCCATGAAATTTGGTTCTTGGACTTATGATGGATCACAGGTTGATATA ATTCTAGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAGATT GTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGGTATCCGTATGTC ACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCTTATAATACCC TGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAATGAAGGTGAA AAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTGGTTATTGAA GAGATCATACCATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTGGTATTTACC ATGATTTTTGTGACACTGTCAATTATGGTAACCGTCTTCGCTATCAACATTCATCATCGT TCTTCCTCAACACATAATGCCATGGCGCCTTTGGTCCGCAAGATATTTCTTCACACGCTT CCCAAACTGCTTTGCATGAGAAGTCATGTAGACAGGTACTTCACTCAGAAAGAGGAAACT GAGAGTGGTAGTGGACCAAAATCTTCTAGAAACACATTGGAAGCTGCGCTCGATTCTATT CGCTACATTACAAGACACATCATGAAGGAAAATGATGTCCGTGAGGTTGTTGAAGATTGG AAATTCATAGCCCAGGTTCTTGATCGGATGTTTCTGTGGACTTTTCTTTTCGTTTCAATT GTTGGATCTCTTGGGCTTTTTGTTCCTGTTATTTATAAATGGGCAAATATATTAATACCA GTTCAAATTGGAAATGCAAATAAGTGA Clone variation with respect to NM_000745.3 1386 t=>a >OriGene 5' read for NM_000745 unedited
TATAGGCGGCCGCGAATTCGGCACGAGGCTGCTGTCCCGGCGGGAGCTGTGGCGCGGAGC GGCCCCTCTGCTGCGTCTGCCCTCGTTTTGTCTCACGACTCACACTCAGTGCTCCATTCC CCAAGAGTTCGCGTTCCCCGCGCGGCGGTCGAGAGGCGGCTGCCCGCGGTCCCGCGCGGG CGCGGGGCGATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTC CAGCTGGTCGCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTA TCTGAACCTTCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGAC TACGAAAGATGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGA CTTGCAATATCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTC TGGTTGAAACAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGA ATAAAAGTTATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGAT AATGCAGATGGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACT GTCACCTGGACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTT CCATTTGACCTTCAGAACTGTTCCATGANATTTGGTTCTTGGACTTATGATGGATCACAG GTTGATATATTNCTAGAGGACAAGATGTAGACCAGAGAGATTTTTTTGATATGGAGATGG GNAGATTGTGAGTGCACAGGGAGCAAAGGAACAGACCGNCAGCTGTGGCTGGTTTCGNAT GTCCT >Forward primer walk for NM_000745 unedited
AATTGGGGCCTTAGTTCCGGTTTATTTAACCCAGGTTCAGGAATATTAATACCAGTCTCA AATTGGNAAATGCAAATAATTGAAGCCTCCCAGGGNACTGAAGTATATACATTTAGTTAA CACACATATATCTGATGGCACCTATAAAATTATGAAAATGTAAGTTATGTGTTAAATTTA GTGCAAGCTTTAACAGACTAAGTTGCTAACCTCAATTTATGTTAACAGATTATCCATTTG AACAGTTGGCTGTATGACTGAAGTAATAACTGATGAGATACATTTGATCTTGTAAAAATA GCAAAATATTATCTGAACTGGACTAGTGAAAAATCTAGTATTTGTATCCTGGCAAATAAT ACTAATTTATAATCCACAGTAAAGTTCATCCTTTGACTGTGCTGGAGAATTCCAGTTGTA TTTGAAGACTGATTTTAAAACTTTTCTGCATTTGGTAAAGGTATGTAAACTTTTCTGCAC TCACTGAGTAACAGCTAATCTTTATATCATATTATACTGTTATATTTTGAAAAACTGACT ACCTGATATAATTCCTTATTGCGATGTTTGGTATAATGACTACTTATTGTGTCCTGCTTC CGGTGGCCTACACCTGTTATCTCGGTGTGTTGACGTTACAGACTTCCTCGGGTAACCATA GGGGCGCGAACGCAAGGAGCCACAGCCGGGGTAGCTATGGCATATGATTGTGTTATGCCA GTAACTATCCTAGGTCGTTAACTTTTTATGCCAGCCATTTATATGCCCGGACCCCTGTTG CAACAATTTTTCCAACAGTCTTCCTATTACTTGTCAATAGGGGCGGGAGTACAGCGGGTC GGTCTGGCCGCCTATTTCCCCCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000745 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000745.2, NP_000736.2 |
| RefSeq Size | 2468 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 1138 |
| UniProt ID | P30532 |
| Domains | Neur_chan_memb, Neur_chan_LBD |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Gene Summary | The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010] Transcript Variant: his variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207624 | CHRNA5 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5) |
CNY 3,656.00 |
|
| RC207624L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207624L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5), mGFP tagged |
CNY 5,890.00 |
|
| RG207624 | CHRNA5 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5) |
CNY 5,256.00 |
