TRPV4 (NM_147204) Human Untagged Clone
CAT#: SC122052
TRPV4 (untagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2
CNY 5,944.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BCYM3; CMT2C; HMSN2C; OTRPC4; SMAL; SPSMA; SSQTL1; TRP12; VRL2; VROAC |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC122052 sequence for NM_147204 edited (data generated by NextGen Sequencing)
ATGGCGGATTCCAGCGAAGGCCCCCGCGCGGGGCCCGGGGAGGTGGCTGAGCTCCCCGGG GATGAGAGTGGCACCCCAGGTGGGGAGGCTTTTCCTCTCTCCTCCCTGGCCAATCTGTTT GAGGGGGAGGATGGCTCCCTTTCGCCCTCACCGGCTGATGCCAGTCGCCCTGCTGGCCCA GGCGATGGGCGACCAAATCTGCGCATGAAGTTCCAGGGCGCCTTCCGCAAGGGGGTGCCC AACCCCATCGATCTGCTGGAGTCCACCCTATATGAGTCCTCGGTGGTGCCTGGGCCCAAG AAAGCACCCATGGACTCACTGTTTGACTACGGCACCTATCGTCACCACTCCAGTGACAAC AAGAGGTGGAGGAAGAAGATCATAGAGAAGCAGCCGCAGAGCCCCAAAGCCCCTGCCCCT CAGCCGCCCCCCATCCTCAAAGTCTTCAACCGGCCTATCCTCTTTGACATCGTGTCCCGG GGCTCCACTGCTGACCTGGACGGGCTGCTCCCATTCTTGCTGACCCACAAGAAACGCCTA ACTGATGAGGAGTTTCGAGAGCCATCTACGGGGAAGACCTGCCTGCCCAAGGCCTTGCTG AACCTGAGCAATGGCCGCAACGACACCATCCCTGTGCTGCTGGACATCGCGGAGCGCACC GGCAACATGCGGGAGTTCATTAACTCGCCCTTCCGTGACATCTACTATCGAGGTCAGACA GCCCTGCACATCGCCATTGAGCGTCGCTGCAAACACTACGTGGAACTTCTCGTGGCCCAG GGAGCTGATGTCCACGCCCAGGCCCGTGGGCGCTTCTTCCAGCCCAAGGATGAGGGGGGC TACTTCTACTTTGGGGAGCTGCCCCTGTCGCTGGCTGCCTGCACCAACCAGCCCCACATT GTCAACTACCTGACGGAGAACCCCCACAAGAAGGCGGACATGCGGCGCCAGGACTCGCGA GGCAACACAGTGCTGCATGCGCTGGTGGCCATTGCTGACAACACCCGTGAGAACACCAAG TTTGTTACCAAGATGTACGACCTGCTGCTGCTCAAGTGTGCCCGCCTCTTCCCCGACAGC AACCTGGAGGCCGTGCTCAACAACGACGGCCTCTCGCCCCTCATGATGGCTGCCAAGACG GGCAAGATTGGGAACCGCCACGAGATGCTGGCTGTGGAGCCCATCAATGAACTGCTGCGG GACAAGTGGCGCAAGTTCGGGGCCGTCTCCTTCTACATCAACGTGGTCTCCTACCTGTGT GCCATGGTCATCTTCACTCTCACCGCCTACTACCAGCCGCTGGAGGGCACACCGCCGTAC CCTTACCGCACCACGGTGGACTACCTGCGGCTGGCTGGCGAGGTCATTACGCTCTTCACT GGGGTCCTGTTCTTCTTCACCAACATCAAAGACTTGTTCATGAAGAAATGCCCTGGAGTG AATTCTCTCTTCATTGATGGCTCCTTCCAGCTGCTCTACTTCATCTACTCTGTCCTGGTG ATCGTCTCAGCAGCCCTCTACCTGGCAGGGATCGAGGCCTACCTGGCCGTGATGGTCTTT GCCCTGGTCCTGGGCTGGATGAATGCCCTTTACTTCACCCGTGGGCTGAAGCTGACGGGG ACCTATAGCATCATGATCCAGAAGATTCTCTTCAAGGACCTTTTCCGATTCCTGCTCGTC TACTTGCTCTTCATGATCGGCTACGCTTCAGCCCTGGTCTCCCTCCTGAACCCGTGTGCC AACATGAAGGTGTGCAATGAGGACCAGACCAACTGCACAGTGCCCACTTACCCCTCGTGC CGTGACAGCGAGACCTTCAGCACCTTCCTCCTGGACCTGTTTAAGCTGACCATCGGCATG GGCGACCTGGAGATGCTGAGCAGCACCAAGTACCCCGTGGTCTTCATCATCCTGCTGGTG ACCTACATCATCCTCACCTTTGTGCTGCTCCTCAACATGCTCATTGCCCTCATGGGCGAG ACAGTGGGCCAGGTCTCCAAGGAGAGCAAGCACATCTGGAAGCTGCAGTGGGCCACCACC ATCCTGGACATTGAGCGCTCCTTCCCCGTATTCCTGAGGAAGGCCTTCCGCTCTGGGGAG ATGGTCACCGTGGGCAAGAGCTCGGACGGCACTCCTGACCGCAGGTGGTGCTTCAGGGTG GATGAGGTGAACTGGTCTCACTGGAACCAGAACTTGGGCATCATCAACGAGGACCCGGGC AAGAATGAGACCTACCAGTATTATGGCTTCTCGCATACCGTGGGCCGCCTCCGCAGGGAT CGCTGGTCCTCGGTGGTACCCCGCGTGGTGGAACTGAACAAGAACTCGAACCCGGACGAG GTGGTGGTGCCTCTGGACAGCATGGGGAACCCCCGCTGCGATGGCCACCAGCAGGGTTAC CCCCGCAAGTGGAGGACTGATGACGCCCCGCTCTAG Clone variation with respect to NM_147204.2 670 a=>c >OriGene 5' read for NM_147204 unedited
GATATTTGTAATCACGACTTCACTATAGGGCGGCACGCGCAATTCGGCACGAGGTACCCT GGTACTCCATCACTAACCTGCTGTGTGACCTCAGGCTGAGCAGTGCAGACGGGCCTGGGG CAGGCATGGCGGATTCCAGCGAAGGCCCCCGCGCGGGGCCCGGGGAGGTGGCTGAGCTCC CCGGGGATGAGAGTGGCACCCCAGGTGGGGAGGCTTTTCCTCTCTCCTCCCTGGCCAATC TGTTTGAGGGGGAGGATGGCTCCCTTTCGCCCTCACCGGCTGATGCCAGTCGCCCTGCTG GCCCAGGCGATGGGCGACCAAATCTGCGCATGAAGTTCCAGGGCGCCTTCCGCAAGGGGG TGCCCAACCCCATCGATCTGCTGGAGTCCACCCTATATGAGTCCTCGGTGGTGCCTGGGC CCAAGAAAGCACCCATGGACTCACTGTTTGACTACGGCACCTATCGTCACCACTCCAGTG ACAACAAGAGGTGGAGGAAGAAGATCATAGAGAAGCAGCCGCAGAGCCCCAAAGCCCCTG CCCCTCAGCCGCCCCCCATCCTCAAAGTCTTCAACCGGCCTATCCTCTTTGACATCGTGT CCCGGNGCTCCACTGCTGACCTGGACGGGCTGCTCCCATTCTTGCTGACCCANCAGAAAC GCCTAACTGATGANGAGTTNCGAGAGCCATCTACGGGGAAGACCTGCCTGCCCAAGGCCT TTGCTGACCTGAGCAATGGCCGCNACGACACCATCCCNTGTGCTGCTGGACATCGCGNAG CGCCACGGGCACATGCNGGAGTTCATTACTCGCCCTTTCGTGACTCTACTTTCGAGTCAG ACAGCCCTGCCATCGCCATGAACGTCGCTGCAACACTACGTGGAACTTCTCGTGCCCAGG AAGCTGATGTCCACGCCAAGCCCTGGGCGCCTTTTCCACCCCAGGTGAAGGGGGCTACCT CTACTTTGGNACCTGCCCCTGTCCTT >OriGene 3' read for NM_147204 unedited
TTACTTTGGACNCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAATGAA TAAACATTTATTGAGCACCGGCAAATCCCAGACACTACAGAACACACAGAAGGCATGGCC CCACGCCGAGGGCCCCAGCCCCTTGCAAAGCTGCCACGCTGCCAAAAATGGTGGCGCATG CAGCTCAGGCGCAGGCTGAGGCTGGGGCTTGGCCGGGCAGTGCACTTGGAACGGGGTCCT AAGGCCTCTGCCAGGTTCCAGCTGGGGCAGGGGTCACGTCGCTTCCTGAGAGCAGAGCAA ATAAATAATGGAGAGGCAGGGGCTGGGGCCTGAGGTGGAGGGGCTCTGGCGTTGGCTTAT GTGACTCCATAGGAGCAAGACAGGTGGCCGGGAGCCCCCACCCCAGGGTGGGGAGGCAGA GCCACGGGACCACAGGGTCCTGGGGCCTCCCTGGCACCTCCACTGGTCCCTCGCCTCTGG GGCCAAAGCAGGGTGTGGGGGGACACCCCAGAAGGCACTGCTGAAATGCGGCTGGACTAG AAATGAGTGGGCAGAGAAGCTGGGGCTGGGCTGCAGTCCCTAGAGCGGGGCGTCATCAGT CCTCCACTTGCGGGGGTAACCCTGCTGGTGGCCATCGCAGCGGGGGTTCCCCATGCTGTC CAGAGGCACCACCACCTCGTCCGGGTTCGAGTTCTTGTTCAGTTCCACCACGCGGNGTAC CACCGAGGACCAGCGATCCCTGCGGAGGCGGCCCACGGTATGCGAGAAGCCATAATACTG GTAGGTCTCATTCTTGCCCGGGTCCTCGTTGATGATGCCCAAGTTCTGGTTCCAGTGAGA CCAGTTCACCTCATCCACCCTGAAGCACACCTGTCGTCAGAAGTGCCGTCCGAGCTTTGC CCACGGTGACATCTCCCAGACGGAAGCCTTCTCAGAATACGGGAAGAGCGT |
Restriction Sites | NotI-NotI |
ACCN | NM_147204 |
Insert Size | 3150 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_147204.1, NP_671737.1 |
RefSeq Size | 3057 bp |
RefSeq ORF | 2436 bp |
Locus ID | 59341 |
UniProt ID | Q9HBA0 |
Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
Gene Summary | This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1, resulting in an isoform (b) that lacks an internal segment compared to isoform a. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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TRPV4-mediated endothelial Ca2+ influx and vasodilation in response to shear stress
,Suelhem A Mendoza, Juan Fang, David D. Gutterman, David A Wilcox, Aaron H Bubolz, Rongshan Li, Makoto Suzuki, and David X. Zhang,
Am J Physiol Heart Circ Physiol, Dec 2009; 10.1152/ajpheart.00854.2009
[TRPV4]
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Other Versions
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RC220097 | TRPV4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2 |
CNY 5,944.00 |
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RC220097L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2, Myc-DDK-tagged |
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RC220097L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2, mGFP tagged |
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RG220097 | TRPV4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2 |
CNY 7,544.00 |