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GRIK1 (NM_175611) Human Untagged Clone
CAT＃: SC121962
GRIK1 (untagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2
CNY 9,952.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EAA3; EEA3; GLR5; GluK1; gluR-5; GLUR5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC121962 sequence for NM_175611 edited (data generated by NextGen Sequencing)
ATGGAGCACGGCACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCA CTCCTCTATTTCCTCTGCTATATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGA GGGATTTTTGAAACAGTGGAAAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTT GCAGTCACCAGCATTAACAGAAACCGAACCCTGATGCCTAACACCACATTAACCTATGAC ATCCAGAGAATTAACCTTTTTGATAGTTTTGAAGCCTCGCGGAGAGCATGTGACCAGCTG GCTCTTGGTGTGGCTGCTCTCTTTGGCCCTTCCCATAGCTCCTCCGTCAGTGCTGTGCAG TCTATTTGCAATGCTCTCGAAGTTCCACACATACAGACCCGCTGGAAACACCCCTCGGTG GACAACAAAGATTTGTTTTACATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCG ATCCTGGATCTGGTCCTCTATTACAACTGGAAAACAGTGACCGTGGTGTATGAAGACAGC ACAGGTCTAATTCGTCTACAAGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATC AAAATCCGCCAGCTGCCCTCTGGGAATAAAGATGCCAAGCCTTTACTCAAGGAGATGAAG AAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCACATGAAACAGCCGCTGAAATCCTT AAGCAGATTCTGTTCATGGGCATGATGACCGAGTACTATCACTACTTTTTCACAACCCTG GACTTATTTGCTTTGGATCTGGAACTCTATAGGTACAGTGGCGTAAACATGACCGGGTTT CGGCTGCTTAACATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAG AGACTGCAGGCCCCACCCAGGCCCGAGACTGGCCTTTTGGATGGCATGATGACAACTGAA GCGGCTCTGATGTACGATGCTGTGTACATGGTGGCCATTGCCTCGCACCGGGCATCCCAG CTGACCGTCAGCTCCCTGCAGTGCCATAGACATAAGCCATGGCGCCTCGGACCCAGATTT ATGAACCTGATCAAAGAGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAA ACCAATGGCTTGAGGAAGGATTTTGATCTGGATATTATTAGTCTCAAAGAGGAAGGAACT GAAAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGAC AAGTCCAGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTG GAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTT GAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGAT GTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGGG ATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACC TACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATT CTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCT CCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTG ATTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGAC GTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATG CAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGG TGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACA GTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATA GAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCC ACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAAC AGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACC AGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGAC TCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATT GCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGT GGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATT GGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGA GAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGTGTCTCTCTTTCAACGCT ATCATGGAAGAACTGGGAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGA ACTAAGGGGAAATCTTCCTTCACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGA AAAGAGACTGTGGCGTGA Clone variation with respect to NM_175611.2 522 a=>c;1173 c=>t >OriGene 5' read for NM_175611 unedited
GCTTGCTCAGAGCCCCTGCACCAACTCACCCTGTACCCTCTCTCCTTCTTCGTTAGTCTT CTTTCCCCCTTTTCCCTCCTCTGTCTGTGCCTATCCCCCGACTTTTGCATCTGACCAAAG GACGAATGAGGGAGACGTTCCTGCAGATCGGGGCAGCAACTTTCCTCAGCTGGTCTCTGG GCTCCGGGAGCCAGAGAGCGCTGATCCTCCGCGGTCTGCGGCCCATGGAAGAGGAGGAGG AGGAGCCGTGATGGGCTAGCGACAGCACTGAGGAGCCCCGAGAGAGCTCAGCCTTGCCAG CCAGCTCCGCGGTCCCACGCGGGTTCCCTCGAGCTCGCTCCGTGGGGAGCGCGCAGCGTG CTTGGAACCGGAGCATCCAGAGAGGATGAGGCGGGGACCCGGCCCAAGTTGGGTGCATCT CTCGGGCGTCCGGCAGCGGCTGTATCTCGGCATGAATTAAGAAGCTAGGAAGATGGAGCA CGGNACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTA TTTCCTCTGCTATATCCTCCCTCAGACCGCCCCGNCAGTACTCAGGATCGGAGGGATTTT TGAAACAGTGGAAAATGAGCCTGTTAATGGTGAAAGAATAGCTTTCAAGTTTGCAGTCAC CAGCATTAACAGAAACCCGACCCTGATGCCCTACACCACATTAACCTATGACATCCAGAG AATTACCTTTTTGATAGTTTTGAAACCTGCCGAGAGCATGTGACCAGCTGGGCTCTGTGG GGCTGCTCTCTTTGGCCCTTCCATAACTCCTCCGTCATGCTGGGCAGNCTATTG >OriGene 3' read for NM_175611 unedited
TTAAAACCTGTTGTTTTCATTATTATTTAGNAAGGCACACACAACAATTTATTGGGGGAA AAAGAAAAAATGCAAAATAATTGAGCATACAAATTTATTTTAAAAGAACTGGTGTCACTT CCCAAGTCATATCTATGTGAGCTTTTTAAACTTTTGTATTTCTTATATCTGTATTCATAA TCAGAGTTATGGATATAGATATATGGAAACACATCACACACTCCTCAGAAATCCTTTCTC CAAAAATCTGTAGGGAATGCATCCTTTTTCTTCCTACAGGCGTTTCCTTGGATCACGCCA CAGTCTCTTTTCTCTGAGTTCGTCTCTGATGACAAGTAAGGATACTTGTGAAGGAAGATT TCCCCTTAGTTCTTGACTTTTTCTTTATTTTTTTCTGATTCTTCAGTGAGATTCCCAGTT CTTCCATGATAGCGTTGAAAGAGAGACACTGTTCAATATCATTATTCTTCCGTGATTTGT ATATGAATTCTCCAATAGCTACAAATACAGAAAGGACCAGTCCGGCAGCCAGAACAATGA AGATGCCTCCAATATTTTCCACTCCCAGGGCACTGGCTTCTTTGTTGTCTTCCTCGGGGC AGCCATTCCCACGCCACCACTTCTCTTTCATCATATGCAGCTTCCCTTCTTCTTGGAGTT GAAGAAATAGCATAGTAATTNTATCCCGGGTAGGAGAACCAATAGGTGTTCCCACTNCCG TACCTTTGNAGTAATGAGGCCCCCGATCTGAGTGAGGTTGCAGTTTCTCTGCGTCACATA CTCAATGCTGGTGGACTNCATCAGCAGCGCGTAGTCTGTGGTGAGCACTCTCTGGATCCC CTCATCACTGTTTCTTACCAGGGCGGTCTGCTGCCTGCTGCTCATGAAAGCCACATCTTC TNATAGGTGGAGAATTTTGATTTCTTGAAGAAGTCATTGTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_175611 |
| Insert Size | 3870 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175611.2, NP_783300.1 |
| RefSeq Size | 3489 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 2897 |
| UniProt ID | P39086 |
| Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2), also known as EAA3A, lacks an alternate in-frame exon in the 3' coding region compared to variant 3. It encodes isoform 2, which is shorter than isoform 3. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212614 | GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2 |
CNY 10,600.00 |
|
| RC212614L3 | Lenti-ORF clone of GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2 |
CNY 11,400.00 |
|
| RC212614L4 | Lenti-ORF clone of GRIK1 (mGFP-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2 |
CNY 11,400.00 |
|
| RG212614 | GRIK1 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2 |
CNY 10,450.00 |
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