United States
Germany
Japan
United Kingdom
China
SPHK1 (NM_182965) Human Untagged Clone
CAT＃: SC121602
SPHK1 (untagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2
CNY 5,488.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPHK |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_182965, the custom clone sequence may differ by one or more nucleotides
ATGTCCGCTCAAGTTCTGGGATTTTTACGCAGCTGGACTCCCCTCCCCCTGGCAGCCCCGAGGGGTCCAG CCGCCGCAGGGAATGACGCCGGTGCTCCTGCAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCG GCCCTGCGACGCCCGCCTGGGCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCC CCCACAGCGCCAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCCCC GGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGGGCAAGGCCTT GCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACT GAGCGGCGGAACCACGCGCGGGAGCTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCA TGTCTGGAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCATGGAGCGGCCTGACTGGGAGACCGCCAT CCAGAAGCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCT GGCTATGAGCAGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGT CACCCATGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGCCTCTTCTCTGTGCTCAGCCTGGCCTG GGGCTTCATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGGGGGAGATGCGCTTCACTCTG GGCACCTTCCTGCGTCTGGCAGCCCTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAG TGGGTTCCAAGACACCTGCCTCCCCCGTTGTGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACT GGAGGAGCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCACTGCTG CACTCGCACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGT TCTACGTGCGGGCGGGAGTGTCTCGTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGAAGGGCAGGCA TATGGAGTATGAATGCCCCTACTTGGTATATGTGCCCGTGGTCGCCTTCCGCTTGGAGCCCAAGGATGGG AAAGGTGTGTTTGCAGTGGATGGGGAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACT ACTTCTGGATGGTCAGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGA AGAGCCCTTATGA >OriGene 5' read for NM_182965 unedited
TCTCTACCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGCGACTGGGTAGGGCCGCCCACCCTGCCTTCGCGCCGCTCGTGGC TCCTGTGCGGCCCGCCCTCGCGGGGCCCCGGGAACTGGGCCACTTGTCGCTTGGGCGAGA GCAGGCGGCAGCTGGTGGCCCGGTTCGGGCTTGGCTTTGGCGCGACCCGGGAGCGGCTCC CACGAGCGCCGCGCGCGTCGCAACGGAGCGGGGCCCTGAGAAGCGCGCGCCGCGGCTCCC ACCGCTCTGGAGCTCCGGGCAGGGGACACGGCAACCTGGATGGCTGGGGCAGGGATCCTC TCCCAGAACTTCGTGCCCAGCAATGTCCGCTCAAGTTCTGGGATTTTTACGCAGCTGGAC TCCCCTCCCCCTGGCAGCCCCGAGGGGTCCAGCCGCCGCAGGGAATGACGCCGGTGCTCC TACAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCGGCCCTGCGACGCCCGCCT GGGCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCCCCCACAGC GCCAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCC CCGGAGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCAGCGGCAA GGGCAAGGGCCTGCAGCTCTTCCAGAGTCACGTGCAGCCCCTTTTGGCTTGAAGCTGAAT CTCCTTACGCTGATGCTCACTGAGCGGCGGACCACGCGCGGGAGCTGGTGCGGTCCGAGA ACTGGCT >OriGene 3' read for NM_182965 unedited
TTGCACCATTGGGCGATGTGCAACTTCCCAGGNCCAGNAAAGCACTGGGGAAGGGTCACA GGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAG TCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAGTCA CTTTATTTGGATTTGGTTCCGGGGGGGGGGGTCTCAAAACAAACTAAAAGGCCTTACATA GGCAGCTGGGCCCAACCCGCTGGGCTCCTGACCCAAGACTTTATTCTGGCCCGTCCCCCC AAAACATAACCCCCACCTTCTCACCCTTTTCCAAAAGAATCTCCCCCCCCCCCACAAGAA CTGTGGACAGGCCCTGCAACCCTAAGGAAAGAAGAAAGGTCCCGCAAGTAAACACTAAAG CACAGCGCCGCCCAAGGGTCATAAAGGCTCTTCTGGCGGTGGCATCTGCTGGGGCTTCCA GCTGGGCGGGGGCTCCACGCCACCCCTGACCATCCAAAAATATTTTGGGTGCACCTGGCC CTGCACGGCCTCCCTAACCATCAATTCCCCATCCACTGGAAACACACCTTTCCCATCCTT GGGCTCCAAGCGGAAGGCGACCACGGGCACCTATACCAAGTAGGGGCATTCATACTCCAT ATGCCCGCCCTTCTCCATGGCCAGGAAGAAGCGCAACAACATGGCACGAGACACTCCCGC CCCCACGTAAAACAAATGCCTGACCCCCGCTGCACAACGGCCCATGGGTGCAGCAAACAT CTCACTGCCCACGTGCCAATGCCGCAATGCCACGACTATCACAAAATCCTCGTCGGGCCC ACTGTCCAGTGAGAGGGCCTGGCTCCTCCCATGGCCAAGGTGTTGCATCTACCGGGCCCT GCTTGGACCCAACGGGGA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_182965 |
| Insert Size | 2200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182965.1, NP_892010.1 |
| RefSeq Size | 2174 bp |
| RefSeq ORF | 1413 bp |
| Locus ID | 8877 |
| UniProt ID | Q9NYA1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway |
| Gene Summary | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017] Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207086 | SPHK1 (Myc-DDK-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 5,488.00 |
|
| RC207086L1 | Lenti-ORF clone of SPHK1 (Myc-DDK-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 7,888.00 |
|
| RC207086L2 | Lenti-ORF clone of SPHK1 (mGFP-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 5,890.00 |
|
| RC207086L3 | Lenti-ORF clone of SPHK1 (Myc-DDK-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 5,890.00 |
|
| RC207086L4 | Lenti-ORF clone of SPHK1 (mGFP-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 5,890.00 |
|
| RG207086 | SPHK1 (tGFP-tagged) - Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 7,088.00 |
|
| SC323952 | SPHK1 (untagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2 |
CNY 5,488.00 |
