ARHGEF1 (NM_198977) Human Untagged Clone

CAT#: SC121265

ARHGEF1 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 3



  "NM_198977" in other vectors (4)

CNY 6,448.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-ARHGEF1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GEF1; IMD62; LBCL2; LSC; P115-RHOGEF; SUB1.5
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_198977, the custom clone sequence may differ by one or more nucleotides


ATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCA
TCGGGGCTGAGGATGAGGATTTTGAGAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCA
GAGCCTGGAGCAGGTGAAGCGGCGCCCAGCCCACCTCATGGCCCTCCTGCAGCACGTGGCCCTGCAGTTT
GAGCCAGGACCCCTGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACTAGGG
CTGACCTCATCTCCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAGCCGT
GGGCCGGCAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCTGGCC
CAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTGGCGGAGCGGC
TGCTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGGTCAA
CGCCATTGGCCTGTACATGCGCCACCTTGGGGTGCGGACCAAGAGTGGAGACAAGAAGTCGGGGAGGAAC
TTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGGACGAGCCTGCCAAGACCAAGAAGGGGCTGAGCA
GCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAGCAGA
GGTTGATGCCGAGAAGCCAGGTGCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCGGAAT
ATCGGGGCTCCTGGGCAGGACACCCCTGGAGTCTCTCTGCACCCTCTGTCCCTGGACAGCCCAGACCGGG
AACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGAGCCTGGAGTCCTT
GGCGCCCCCAGAGAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAGGGGGAGCCG
GGGCGGTCGGGACTGGAGCTTGAACCAGAAGAGCCTCCCGGCTGGCGGGAACTCGTCCCCCCAGACACCC
TGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTCATCAGCGAGCTGCTGGTGACAGAGGCGGC
CCACGTGCGCATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTTCCCC
TTGGAGGAGCTGCAGAACATCTTCCCCAGCCTGGACGAGCTCATCGAGGTGCATTCCCTGTTCCTCGATC
GCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGAGACGTGCTGCTGGCCCGGTT
TGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCATTTGCCTTA
GAGCAGCTCAAAGCCAAGCAACGCAAGGACCCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGCCGCC
CGCGGTGCCGCCGCCTGCAGCTGAAGGACATGATCCCCACGGAGATGCAGCGGCTGACCAAGTACCCCCT
GCTCCTGCAGAGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGCCGAG
TGCTGCCGGGAAATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTCAAGG
ACTATCAGCGGCGCCTGGACTTGTCCCACCTTCGGCAGAGCAGCGACCCTATGCTGAGCGAGTTCAAGAA
CCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGTGGCGGGTGACTAAGGACAAGGCA
GTGGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTGCTGC
TCAAGTCCCATAGCCGGACACTGACGCCCACGCCCGATGGCAAGACCATGCTGCGGCCCGTGCTGCGGCT
CACCTCCGCCATGACCCGCGAGGTGGCCACCGATCACAAAGCCTTCTACGTCCTTTTTACCTGGGACCAG
GAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATCACTG
AGACTGCCGGATCCCTGAAAGTCCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCAGCAC
CCGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGTGGCCGAGAGACGTCTCCAGCTGATGCCCGG
ACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAGGGCCAGCTCGCTGCCACGG
CCCTTCGGAAAGTGCTGTCCCTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGCCTCC
TCGAGATGGGGATGGGGTCCCAGGGGGCGGCCCCCTGAGCCCAGCACGGACCCAGGAAATCCAGGAGAAC
CTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGCCTGAGACCCC
TCCTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACTTGA


>OriGene 5' read for NM_198977 unedited
GCACGAGGGCGCAAAGGTGGACTCAGGGCGGCTAGAGCGACGCGGCGGCAGGGGTGGGGA
GAGTGCGGAGCCCGAGCGCGGAGGCTTCGGTTCCGGTGGCGGCGATGGCTTCTCTTTCCA
CCTGGAGCAGCCCTGCAGAGCCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGGCCTCCC
CAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCATCGGGGCTGAGGATGAGGATT
TTGAGAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGC
AGGTGAAGCGGCGCCCAGCCCACCTCATGGCCCTCCTGCAGCACGTGGCCCTGCAGTTTG
AGCCAGGACCCCTGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTTGACC
GCACTAGGGCTGACCTCATCTCCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGC
AAAGCCAGCAGGTAGCCGTGGGCCGGCAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGG
GCATGACGCCCTGNGAGCAGGAGCTGGCCCAGCTGGAGG
Restriction Sites NotI-NotI     
ACCN NM_198977
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198977.1, NP_945328.1
RefSeq Size 3137 bp
RefSeq ORF 2640 bp
Locus ID 9138
UniProt ID Q92888
Protein Pathways Regulation of actin cytoskeleton, Vascular smooth muscle contraction
Gene Summary Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) has an alternate 5' exon and lacks an in-frame internal exon, as compared to variant 1. The encoded isoform 3 thus has a shorter N-terminus and lacks an internal segment, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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