PTK9 (TWF1) (NM_198974) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A6, MGC23788, MGC41876
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC121172 sequence for NM_198974 edited (data generated by NextGen Sequencing) ATGTTGTATGCAGCAACAAGAGCAACTCTGAAGAAGGAATTTGGAGGTGGCCACATTAAA GATGAAGTATTTGGAACAGTAAAGGAAGATGTATCATTACATGGATATAAAAAATACTTG CTGTCACAATCTTCCCCTGCCCCACTGACTGCAGCTGAGGAAGAACTACGACAGATTAAA ATCAATGAGGTACAGACTGACGTGGGTGTGGACACTAAGCATCAAACACTACAAGGAGTA GCATTTCCCATTTCTCGAGAAGCCTTTCAGGCTTTGGAAAAATTGAATAATAGACAGCTC AACTATGTGCAGTTGGAAATAGATATAAAAAATGAAATTATAATTTTGGCCAACACAACA AATACAGAACTGAAAGATTTGCCAAAGAGGATTCCCAAGGATTCAGCTCGTTACCATTTC TTTCTGTATAAACATTCCCATGAAGGAGACTATTTAGAGTCCATAGTTTTTATTTATTCA ATGCCTGGATACACATGCAGTATAAGAGAGCGGATGCTGTATTCTAGCTGCAAGAGCCGT CTGCTAGAAATTGTAGAAAGACAACTACAAATGGATGTAATTAGAAAGATCGAGATAGAC AATGGGGATGAGTTGACTGCAGACTTCCTTTATGAAGAAGTACATCCCAAGCAGCATGCA CACAAGCAAAGTTTTGCAAAACCAAAAGGTCCTGCAGGAAAAAGAGGAATTCGAAGACTA ATTAGGGGCCCAGCGGAAACTGAAGCTACTACTGATTAA Clone variation with respect to NM_198974.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_198974 unedited AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGNAAAATGTTAAAGAGATC TTTGCCAGAGCCAGAAATGGAAAGTACAGACTTCTGAAAATATCTATTGAAAATGAGCAA CTTGTGATTGGATCATATAGTCAGCCTTCAGATTCCTGGGATAAGGATTATGATTCCTTT GTTTTACCCCTGTTGGAGGACAAACAACCATGCTATATATTATTCAGGTTAGATTCTCAG AATGCCCAGGGATATGAATGGATATTCATTGCATGGTCTCCAGATCATTCTCATGTTCGT CAAAAAATGTTGTATGCAGCAACAAGAGCAACTCTGAAGAAGGAATTTGGAGGTGGCCAC ATTAAAGATGAAGTATTTGGAACAGTAAAGGAAGATGTATCATTACATGGATATAAAAAA TACTTGCTGTCACAATCTTCCCCTGCCCCACTGACTGCAGCTGAGGAAGAACTACGACAG ATTAAAATCAATGAGGTACAGACTGACGTGGGTGTGGACACTAAGCATCAAACACTACAA GGAGTAGCATTTCCCATTTCTCGAGAAGCCTTTCAGGCTTTGGAAAAATTGAATAATAGA CAGCTCAACTATGTGCAGTTGGAAATAGATATAAAAAATGAAATTATAATTTTGGCCAAC ACAACAAATACAGAACTGAAAGATTTGCCAAAGAGGATTCCCAAGGATTCAGCTCGTTAC CATTTCTTTCTGTTAAACATTCCCATGAAGGAGACTATNTAGAGTCCATAGTTNTTATTT ATTCAATGCCTGGATACACATGCAGTATAAGAGAGCGGATGCTGTATTCTACTGCAAGAG CCGTCTGCTANAAATGTANAAAGACACTACAAATGATGTATTANAAAGACGAGATAACAT GGGATGAGTGACTGCGACTTCTTATGGAGAGTCTCCNAGCAGCTGCCANAGCAAGTTTGC AAACCAAAGTCTGCGGAAAGAGAATA
Restriction Sites	NotI-NotI
ACCN	NM_198974
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_198974.1, NP_945325.1
RefSeq Size	3455 bp
RefSeq ORF	759 bp
Locus ID	5756
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate splice site in the 5' region compared to variant 1, resulting in translation initiation at a downstream start codon and an isoform (2) with a shorter N-terminus compared to isoform 1.

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