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ADHFE1 (NM_144650) Human Untagged Clone
CAT＃: SC120771
ADHFE1 (untagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADH8; HMFT2263; HOT |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_144650 edited
GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTGCCGCCCGAG CCCGGGTCGCGTACTTGCTGAGGCAACTGCAACGCGCAGCCCCCTGGACTTTCACCTTCT GGGAAAACAACAGATTATGCCTTTGAGATGGCTGTTTCAAATATTAGATATGGAGCAGCA GTTACAAAGGAAGTAGGAATGGACCTAAAAAACATGGGTGCTAAAAATGTGTGCTTGATG ACAGACAAGAACCTCTCCAAGCTCCCTCCTGTGCAAGTAGCTATGGATTCCCTAGTGAAG AATGGCATCCCCTTTACGGTTTATGATAATGTGAGAGTGGAACCAACGGATTCAAGCTTC ATGGAAGCTATTGAGTTTGCCCAAAAGGGAGCTTTTGATGCCTATGTTGCTGTCGGTGGT GGCTCTACCATGGACACCTGTAAGGCTGCTAATCTGTATGCATCCAGCCCTCATTCTGAT TTCCTAGATTATGTCAGTGCCCCCATTGGCAAGGGAAAGCCTGTGTCTGTGCCTCTTAAG CCTCTGATTGCAGTGCCAACTACCTCAGGAACCGGGAGTGAAACTACTGGGGTTGCCATT TTTGACTATGAACACTTGAAAGTAAAAATTGGCATCACTTCGAGAGCCATCAAACCCACA CTGGGACTGATTGATCCTCTGCACACCCTCCACATGCCTGCCCGAGTGGTCGCCAACAGT GGCTTTGATGTGCTTTGCCATGCCCTGGAGTCATACACCACCCTGCCCTACCACCTGCGG AGCCCCTGCCCTTCAAATCCCATCACACGGCCTGCGTACCAGGGCAGCAACCCAATCAGT GACATTTGGGCTATCCACGCGCTGCGGATCGTGGCTAAGTATCTGAAGAGGGCCGTCAAC AGCACTGACAAATGAAGAAGAAGAATGAGTCTCAGGGCACCTTCCTGGAGTGGCCCTCGC CATCCAGGGACCAGCGCGTTGCCTCCCCCGGCGCTTTTACATCACATGAGCAGCTGACAG CGTATTTATTATGCCTTTGCTGAAACAGTTGATGAGAAATATGTTTCCCTTCAGTTTTCA TTTAGAAGACAAGGCGTCGTTTGAATAACTTGCCTGATTTTTCTTCCTACCGAGTAGCAT TTCTTTTTTTTTCACCATATCTTTCCCTAAGGCAGCTCCTTATTCTGTAGGAATTGCCAA TGTTGATGTGTTATTGTTAGGTATTTATATCATTCACAGGGCTGTCAGAAATCCCGATGA TCTTGAAGCAAGGTCTCATATGCACTTGGCAAGTGCTTTTGCTGGCATCGGCTTTGGAAA TGCTGGTGTTCATCTGTGCCATGGAATGTCTTACCCAATTTCAGGTTTAGTGAAGATGTA TAAAGCAAAGGATTACAATGTGGATCACCCACTGGTGCCCCATGGCCTTTCTGTGGTGCT CACGTCCCCAGCGGTGTTCACTTTCACGGCCCAGATGTTTCCAGAGCGACACCTGGAGAT GGCAGAAATACTGGGAGCCGACACCCGCACTGCCAGGATCCAAGATGCAGGGCTGGTGTT GGCAGACACGCTCCGGAAATTCTTATTCGATCTGGATGTTGATGATGGCCTAGCAGCTGT TGGTTACTCCAAAGCTGATATCCCCGCACTAGTGAAAGGAACGCTGCCCCAGGAAAGGGT CACCAAGCTTGCACCCCGTCCCCAGTCAGAAGAGGATCTGGCTGCTCTGTTTGAAGCTTC AATGAAACTGTATTAATTGTCATTTTAACTGAAAGAATTACCGCTGGCCATTGTAGTGCT GAGAGCAAGAGCTGATCTAGCTAGGGCTTTGTCTTTTCATCTTTGTGCATAACTTACCTG TTACCAGTATAGGTGGGATATACATTTATCTTGCAGGAAATTCCCCAAAGCTCAGAGTCC AGTTCCTTCCATAAAACAGGCTGGACAAATGACCACTATGTTAGACCCCCAGGCTCGACT TCAGGGGTCAGTGTTCCTGTCCCAAACCCCACACAGAATACTCTGCCTCTGCTTCATGTA GCAAATGAGCAAAAACTCAGTATCTATCAAAAGTGTAAATTATATTTCCTATGCCTAGTA ATTCACTTCATGTCTAAAAATTTATCTGATAGAAACACTAGCACCAGTACATACAGAAGC ATGGCAAGGATGTTTCTGGCAGCACTTTTCTAATAATAAAAGATTTGAAACAACAAAAAA AAAAAAAAAAAA >OriGene 5' read for NM_144650 unedited
CCAACCGGCACACCCCCACCCTCCCCCCCCCCCGTTCAGATTTGNATACAACTCATATAG GCGGCCGCGAAATTCGCACCAGCTGCCGCCCGAGCCCGGGTCGCGTACTTGCTGAGGCAA CTGCAACGCGCAGCCCCCTGGACTTTNNCACCTTCTGGGAAAACAACAGATTATGCCTTT GAGATGGCTGTTTCAAATATTAGATATGGAGCAGCAGTTACAAAGGAAGTAGGAATGGAC CTAAAAAACATGGGTGCTAAAAATGTGTGCTTGATGACAGACAAGAACCTCTCCAAGCTC CCTCCTGTGCAAGTAGCTATGGATTCCCTAGTGAAGAATGGCATCCCCTTTACGGTTTAT GATAATGTGAGAGTGGAACCAACGGATTCAAGCTTCATGGAAGCTATTGAGTTTGCCCAA AAGGGAGCTTTTGATGCCTATGTTGCTGTCGGTGGTGGCTCTACCATGGACACCTGTAAG GCTGCTAATCTGTATGCATCCAGCCCTCATTCTGATTTCCTAGATTATGTCAGTGCCCCC ATTGGCAAGGGAAAGCCTGTGTCTGTGCCTCTTAAGCCTCTGATTGCAGTGCCAACTACC TCANGAACCGGGAGTGAAACTACTGNGGTTGCCATTTTTGACTATGAACACTTGAAAGTA AAAATTGGCATCACTTTCGAGAGCCATCAAACCCACACTGGGACTGATTGATCCTCTGCA CACCCTTCACATGCCTGCCCGAGTGGTCGCCACAGTGGCTTTGATGTGCTTTGCATGCCC TGNAGTCATACACCACCCTTGCCTAACCACCTGCGGAGCCCTGCCCTTCAAATNCCATTA CACGGCCTGCGTACCNGGGCAGCAACCCAATCANTGACATTTGGGCTATTCACGCCCTGC GGATCGGGCCTAAATTTCTGAGAGGGCCGTCAACAGCCTGGCAAATGAAGAGAAAAAGGA CCTTC >OriGene 3' read for NM_144650 unedited
GACTCACTATGGTCGTGGCCGCCACCTAAGATCGGTTTTTTTTTTTTTTTTTTGTTGTTT CAAATCTTTTATTATTAGAAAAGTGCTGCCAGAAACATCCTTGCCATGCTTCTGTATGTA CTGGTGCTAGTGTTTCTATCAGATAAATTTTTAGACATGAAGTGAATTACTAGGCATAGG AAATATAATTTACACTTTTGATAGATACTGAGTTTTTGCTCATTTGCTACATGAAGCAGA GGCAGAGTATTCTGTGTGGGGTTTGGGACAGGAACACTGACCCCTGAAGTCGAGCCTGGG GGTCTAACATAGTGGTCATTTGTCCAGCCTGTTTTATGGAAGGAACTGGACTCTGAGCTT TGGGGAATTTCCTGCAAGATAAATGTATATCCCACCTATACTGGTAACAGGTAAGTTATG CACAAAGATGAAAAGACAAAGCCCTAGCTAGATCAGCTCTTGCTCTCAGCACTACAATGG CCAGCGGTAATTCTTTCAGTTAAAATGACAATTAATACAGTTTCATTGAAGCTTCAAACA GAGCAGCCAGATCCTCTTCTGACTGGGGACGGGGTGCAAGCTTGGTGACCCTTTCCTGGG GCAGCGTTCCTTTCACTAGTGCGGGGATATCAGCTTTGGAGTAACCAACAGCTGCTAGGC CATCATCAACATCCAGATCGAATAAGAATTTCCGGAGCGTGTCTGCCAACACCAGCCCTG CATCTTGGATCCTGGCAGTGCGGGTGTCGGCTCCCAGTATTTCTGCCATCTCCAGGTGTC GCTCTGGAAACATCTGGGCCGTGAAAGTGAACACCGCTGGGGGACGTGAGCACCACAGAT AGGCCATGGGGCACCAGTGGGTGATCCACATTGTNATCCTTTGGCTTATACATCTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_144650 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144650.1, NP_653251.1 |
| RefSeq Size | 1830 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 137872 |
| UniProt ID | Q8IWW8 |
| Domains | Fe-ADH |
| Gene Summary | The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223298 | ADHFE1 (Myc-DDK-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| RC223298L3 | Lenti-ORF clone of ADHFE1 (Myc-DDK-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 7,888.00 |
|
| RC223298L4 | Lenti-ORF clone of ADHFE1 (mGFP-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,990.00 |
|
| RG223298 | ADHFE1 (tGFP-tagged) - Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 7,088.00 |
|
| SC317769 | ADHFE1 (untagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
