DAAM1 (NM_014992) Human Untagged Clone

CAT#: SC120568

DAAM1 (untagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1)



  "NM_014992" in other vectors (6)

CNY 7,512.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-DAAM1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC120568 sequence for NM_014992 edited (data generated by NextGen Sequencing)
ATGGCCCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAAT
AATGATCACCCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACC
ATGGAACCAGCATTGCCCATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTG
GTGGATGAACTGGACCTCACAGACAAACACAGAGAAGCCATGTTTGCACTTCCAGCTGAG
AAAAAATGGCAAATATACTGTAGCAAGAAAAAGGACCAGGAAGAAAACAAGGGAGCTACA
AGTTGGCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCTAGAAAATCTCTGCTG
GCTTTAGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAGACAGCA
CTGAGGACAAAACCAATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGT
ATCCTCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGAATACATACTTCT
CTCATTGGCTGTATAAAGGCGTTAATGAACAACTCTCAAGGCCGGGCTCACGTCCTGGCT
CATTCTGAGAGTATTAATGTAATTGCTCAGAGTCTGAGCACAGAGAACATTAAAACGAAG
GTGGCCGTGCTGGAAATCTTGGGCGCCGTGTGCCTGGTTCCCGGGGGCCACAAGAAGGTT
CTGCAGGCCATGCTGCACTACCAGAAGTATGCCAGCGAAAGGACCCGCTTTCAGACATTA
ATTAACGACTTGGATAAAAGCACTGGGCGGTATCGAGATGAAGTGAGTCTCAAGACTGCC
ATCATGTCCTTCATTAATGCAGTGCTCAGCCAAGGTGCAGGAGTGGAGAGTTTGGACTTT
AGACTTCATCTTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTAATAGATAAATTA
AGGGAACACGAAAATTCAACATTAGATAGGCATTTAGACTTTTTTGAAATGCTCCGAAAT
GAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGTGCA
ACTCAGATGTTTGAGCTGACCAGGAAGAGGCTGACACATAGTGAAGCTTACCCGCATTTC
ATGTCCATCCTGCACCACTGCCTCCAAATGCCTTACAAGAGGAGTGGCAACACTGTTCAG
TACTGGCTACTACTAGATAGAATTATACAGCAGATAGTTATCCAGAATGACAAAGGACAG
GACCCTGACTCCACACCTTTGGAAAACTTTAATATTAAGAATGTCGTACGAATGTTGGTT
AATGAAAATGAAGTTAAGCAGTGGAAAGAACAAGCGGAAAAAATGAGAAAAGAGCACAAT
GAGCTACAGCAGAAACTGGAAAAGAAAGAACGAGAATGTGATGCTAAGACTCAAGAGAAG
GAAGAGATGATGCAGACCTTAAATAAAATGAAAGAGAAACTTGAAAAGGAGACTACTGAG
CATAAGCAAGTCAAGCAGCAGGTGGCGGACCTCACAGCACAGCTCCATGAGCTCAGCAGG
AGGGCCGTCTGTGCTTCAATCCCAGGTGGACCCTCGCCTGGAGCACCAGGAGGGCCCTTT
CCTTCCTCTGTGCCTGGATCTCTCCTTCCTCCCCCACCACCCCCACCTCTACCAGGTGGG
ATGCTTCCCCCTCCACCGCCTCCCCTCCCTCCAGGTGGCCCTCCTCCTCCCCCAGGGCCT
CCTCCCTTAGGGGCAATCATGCCACCTCCTGGTGCTCCAATGGGCCTAGCACTGAAGAAG
AAAAGCATTCCTCAGCCCACAAATGCCCTGAAATCCTTCAACTGGTCTAAACTGCCCGAG
AACAAACTGGAAGGAACAGTATGGACCGAAATTGATGATACAAAAGTCTTCAAAATTCTA
GATCTTGAAGACCTGGAAAGAACCTTCTCTGCCTATCAAAGACAGCAGGATTTCTTTGTG
AACAGTAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACTCTGAGTTCCAAACTT
AAAGTTAAAGAGCTTTCGGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTA
TCGAGGTTGAAATTATCCAATGACGAAATCAAACGGGCAATTCTAACAATGGACGAACAG
GAAGATCTGCCCAAGGACATGTTGGAACAGCTCTTGAAATTTGTTCCTGAAAAAAGTGAC
ATTGACCTATTGGAGGAACATAAACACGAACTGGATCGGATGGCCAAGGCTGATAGGTTC
CTTTTTGAGATGAGCCGAATTAATCACTATCAGCAAAGGTTGCAATCGCTGTACTTCAAA
AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAACCTAAAGTGGAAGCAATTCGTTCTGGC
TCAGAAGAGGTGTTTAGGAGTGGTGCCCTCAAGCAGTTGCTGGAGGTGGTTTTGGCATTT
GGAAATTATATGAATAAAGGTCAAAGAGGGAATGCATATGGATTCAAGATATCTAGCCTA
AACAAAATTGCTGACACAAAATCCAGCATCGACAAAAACATTACCCTTTTGCACTATCTC
ATCACTATTGTGGAAAATAAGTACCCCAGTGTTCTCAATCTAAATGAAGAATTGCGAGAT
ATTCCTCAAGCTGCGAAAGTAAACATGACTGAGCTGGACAAAGAAATAAGTACCTTGAGA
AGTGGCTTGAAAGCAGTAGAGACAGAGCTGGAATATCAGAAGTCTCAGCCCCCACAGCCC
GGAGATAAGTTTGTGTCTGTTGTCAGCCAGTTCATCACAGTAGCCAGCTTCAGCTTCTCT
GATGTTGAAGACCTTCTAGCAGAAGCTAAAGACCTGTTTACTAAAGCAGTGAAGCACTTT
GGGGAAGAGGCTGGCAAAATACAACCAGATGAGTTCTTTGGCATTTTTGATCAATTTCTT
CAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAAAGAAAAAGGAGGAAGAA
GAACGTCGAGCTCGCATGGAAGCTCAGCTCAAAGAACAACGTGAAAGGGAACGTAAAATG
AGAAAAGCTAAAGAGAATAGTGAAGAAAGCGGAGAGTTTGATGACCTTGTTTCAGCTTTA
CGCTCAGGAGAAGTGTTTGACAAAGACCTTTCTAAATTGAAACGGAATCGCAAACGTATT
ACCAACCAGATGACTGACAGCAGCAGAGAGAGACCAATCACAAAACTTAATTTCTAA

Clone variation with respect to NM_014992.1
1389 a=>g
>OriGene 5' read for NM_014992 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCCTACGAGCGGC
TGTGAAGGAAACTGTTTAACCGGATCCCATTGTACCCAGAGTGCAGAGCCGCCTTTCCAG
CATGCAGGGGCTGCTCAGCGTTTAGTCACATCAAGAAATAGAACAGAATTCAGCCATGGC
CCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAATAATGA
TCACCCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACCATGGA
ACCAGCATTGCCCATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTGGTGGA
TGAACTGGACCTCACAGACAAACACAGAGAAGCCATGTTTGCACTTCCAGCTGAGAAAAA
ATGGCAAATATACTGTAGCAAGAAAAAGGACCAGGAAGAAAACAAGGGAGCTACAAGTTG
GCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCTAGAAAATCTCTGCTGGCTTT
AGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAGACAGCACTGAG
GACAAAACCAATGAGGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGTATCC
TCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGATACATACTTCTCTCAT
TGGCTGTATAAAGGGCGTAATGAACAACTCTCAAGGNCGGGCTCACGTNCTGGCTCATTC
TGAGAGTATTAATGGTATTTGCTCAAAGTCTGAGCACAGAGAACATTANNAACGANGGTT
GGCCGTGCTTGGAAATCTTNGGCCGCCGTGTTGCTGGGTTNCCGGGGGCACAAANAAAGT
TCTCGCAGNCCATGCTGCACTACCAAAGTATG
>OriGene 3' read for NM_014992 unedited
TAGCTTTGAACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGTAATC
TTTATCCCTTCAAAAAATCTTCCTTTAAGTCTTGGGTGAGATATTTTATTATTTTTAAGG
TTTATATATCTATATTGTTAGAACAAGCATACTCTGGCAATGTTGTTCTTTAAAAAAAGC
ATGGATTGCATTTATATAGTGTTTTTTTCCAAAAATCTGAAGAAATTATCAAGAGCACTT
GGCAGAGATAACTTGATGAAATCAGGGGAAAAAATTGATGTCCAACTTTTAAATATACAA
AAACTTGATTGACATTCTACATAATTCAAATTGTGTGCAAGATGTTCTCAATAAACATTT
ACAGACTGTATCCCCACACCAATGTCAGAAATTCAAAGCTGGTCCTATTACTGATGTGAT
ACATCAACATCACAATGTCAGCTATCAAAAATCATTTTAAATCTATGTTTTTCCAACAAC
TCCAGTTCCATCAATGAAAATAATTTTAAAGGATATCAAGCTTGGAAACCATAAAATATT
TTGCAAGCTTTTTTTGTTTGTTTTGTTTGTTTCTTCTGTTTTGCCTTTGAATCAGGGAAA
TGTCTCTTATCTCTTTCAGGAGATAGTATTGTGCTTCTGAACAGTATGGCGTTCGGNGAA
AGAAAATGAGCCCACTCAATGTGAATGCATACACGAAAAGTTTTGNAGCACATACATTAA
CATAANATCTTTTAACCATACGTTAATAGGTCTCCAGACAACACTCAGACATCTATATAC
AATTTTTTAATATATACACACATACATTTACATACACACGTTCACACATTCACTCACAGA
TGAAGAANAAATGCCACTNTTTGAATGCCAAGNANTGTAATGAAAACTTCTAGTACTTTT
AAAGGCTGCTATGAGCTCTAAAAAAGAATCATGAAATNAAATAAATTTNGGAATGGNCCT
TCTGCTCTTGAAAATTGGGGGGAAACTAAA
Restriction Sites NotI-NotI     
ACCN NM_014992
Insert Size 4600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014992.1, NP_055807.1
RefSeq Size 4256 bp
RefSeq ORF 3237 bp
Locus ID 23002
UniProt ID Q9Y4D1
Domains FH2
Protein Pathways Wnt signaling pathway
Gene Summary Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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