PLC delta 3 (PLCD3) (NM_133373) Human Untagged Clone

CAT#: SC120539

PLCD3 (untagged)-Human phospholipase C, delta 3 (PLCD3)



  "NM_133373" in other vectors (6)

CNY 2,950.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PLC-delta-3
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_133373 edited
CTCAGAGCCGGGGCCGGGCCAGAGCGGCGCCCCGCTGCCCTGTCCCCGCGTGCAGACCCC
GGGCCCGGCCCCGGCCCCCCGCCAAGCCATGCTGTGCGGCCGCTGGAGGCGTTGCCGCCG
CCCGCCCGAGGAGCCCCCGGTGGCCGCCCAGGTCGCAGCCCAAGTCGCGGCGCCGGTCGC
TCTCCCGTCCCCGCCGACTCCCTCCGATGGCGGCACCAAGAGGCCCGGGCTGCGGGCGCT
GAAGAAGATGGGCCTGACGGAGGACGAGGACGTGCGCGCCATGCTGCGGGGCTCCCGGCT
CCGCAAGATCCGCTCGCGCACGTGGCACAAGGAGCGGCTGTACCGGCTGCAGGAGGACGG
CCTGAGCGTGTGGTTCCAGCGGCGCATCCCGCGTGCGCCATCGCAGCACATCTTCTTCGT
GCAGCACATCGAGGCGGTCCGCGAGGGCCACCAGTCCGAGGGCCTGCGGCGCTTCGGGGG
TGCCTTCGCGCCAGCGCGCTGCCTCACCATCGCCTTCAAGGGCCGCCGCAAGAACCTGGA
CCTGGCGGCGCCCACGGCTGAGGAAGCGCAGCGCTGGGTGCGCGGTCTGACCAAGCTCCG
CGCGCGCCTGGACGCCATGAGCCAGCGCGAGCGGCTAGACCACTGGATCCACTCCTATCT
GCACCGGGCTGACTCCAACCAGGACAGCAAGATGAGCTTCAAGGAGATCAAGAGCCTGCT
GAGAATGGTCAACGTGGACATGAACGACATGTACGCCTACCTCCTCTTCAAGGAGTGTGA
CCACTCCAACAACGACCGTCTAGAGGGGGCTGAGATCGAGGAGTTCCTGCGGCGGCTGCT
GAAGCGGCCGGAGCTGGAGGAGATCTTCCATCAGTACTCGGGCGAGGACCGCGTGCTGAG
TGCCCCTGAGCTGCTGGAGTTCCTGGAGGACCAGGGCGAGGAGGGCGCCACACTGGCCCG
CGCCCAGCAGCTCATTCAGACCTATGAGCTCAACGAGACAGCCAAGCAGCATGAGCTGAT
GACACTGGATGGCTTCATGATGTACCTGTTGTCGCCGGAGGGGGCTGCCTTGGACAACAC
CCACACGTGTGTGTTCCAGGACATGAACCAGCCCCTTGCCCACTACTTCATCTCTTCCTC
CCACAACACCTATCTGACTGACTCCCAGATCGGGGGGCCCAGCAGCACCGAGGCCTATGT
TAGGGCCTTTGCCCAGGGATGCCGCTGCGTGGAGCTGGACTGCTGGGAGGGGCCAGGAGG
GGAGCCCGTCATCTATCATGGCCATACCCTCACCTCCAAGATTCTCTTCCGGGACGTGGT
CCAAGCCGTGCGCGACCATGCCTTCACGCTGTCCCCTTACCCTGTCATCCTATCCCTGGA
GAACCACTGCGGGCTGGAGCAGCAGGCTGCCATGGCCCGCCACCTCTGCACCATCCTGGG
GGACATGCTGGTGACACAGGCGCTGGACTCCCCAAATCCCGAGGAGCTGCCATCCCCAGA
GCAGCTGAAGGGCCGGGTCCTGGTGAAGGGAAAGAAGCTGCCCGCTGCTCGGAGCGAGGA
TGGCCGGGCTCTGTCGGATCGGGAGGAGGAGGAGGAGGATGACGAGGAGGAAGAAGAGGA
GGTGGAGGCTGCAGCGCAGAGGCGGCTGGCCAAGCAGATCTCCCCGGAGCTGTCGGCCCT
GGCTGTGTACTGCCACGCCACCCGCCTGCGGACCCTGCACCCTGCCCCCAACGCCCCACA
ACCCTGCCAGGTCAGCTCCCTCAGCGAGCGCAAAGCCAAGAAACTCATTCGGGAGGCAGG
GAACAGCTTTGTCAGGCACAATGCCCGCCAGCTGACCCGCGTGTACCCGCTGGGGCTGCG
GATGAACTCAGCCAACTACAGTCCCCAGGAGATGTGGAACTCGGGCTGTCAGCTGGTGGC
CTTGAACTTCCAGACGCCAGGCTACGAGATGGACCTCAATGCCGGGCGCTTCCTAGTCAA
TGGGCAGTGTGGCTACGTCCTAAAACCTGCCTGCCTGCGGCAACCTGACTCGACCTTTGA
CCCCGAGTACCCAGGACCTCCCAGAACCACTCTCAGCATCCAGGTGCTGACTGCACAGCA
GCTGCCCAAGCTGAATGCCGAGAAGCCACACTCCATTGTGGACCCCCTGGTGCGCATTGA
GATCCATGGGGTGCCCGCAGACTGTGCCCGGCAGGAGACTGACTACGTGCTCAACAATGG
CTTCAACCCCCGCTGGGGGCAGACCCTGCAGTTCCAGCTGCGGGCTCCGGAGCTGGCACT
GGTCCGGTTTGTGGTGGAAGATTATGACGCCACCTCCCCCAATGACTTTGTGGGCCAGTT
TACACTGCCTCTTAGCAGCCTAAAGCAAGGGTACCGCCACATACACCTGCTTTCCAAGGA
CGGGGCCTCACTGTCACCAGCCACGCTCTTCATCCAAATCCGCATCCAGCGCTCCTGAGG
GCCCACCTCACTCGCCTTGGGGTTCTGCGAGTGCCAGTCCACATCCCCTGCAGAGCCCTC
TCCTCCTCTGGAGTCAGGTGGTGGGAGTACCAGCCCCCCAGCCCACCCACTTGGCCCACT
CAGCCCATTCACCAGGCGCTGGTCTCACCTGGGTGCTGAGGGCTGCCTGGGCCCCTCCTG
AAGAACAGAAAGGTGTTCATGTGACTTCAGTGAGCTCCAACCCTGGGGCCCTGAGATGGC
CCCAGCTCCTCTTGTCCTCAGCCCACCCCTCATTGTGACTTATGAGGAGCAAGCCTGTTG
CTGCCAGGAGACTTGGGGAGCAGGACACTTGTGGGCCCTCAGTTCCCCTCTGTCCTCCCG
TGGGCCATCCCAGCCTCCTTCCCCCAGAGGAGCGCAGTCACTCCACTTGGCCCCGACCCC
GAGCTTAGCCCCTAAGCCCTCCTTTACCCCAGGCCTTCCTGGACTCCTCCCTCCAGCTCC
GGAACCTGAGCTCCCCTTCCCTTCTCAAAGCAAGAAGGGAGCGCTGAGGCATGAAGCCCT
GGGGAAACTGGCAGTAGGTTTTGGTTTTTATTTTTTGAGACAGGGTCTCGCTCCGTCGCC
CAGGCTGGAGTGCAATGTTGCAATCATGGCTCACTGCAGCTTTGAACTCCCAGGCTCAAG
CGATCCTCCCATCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACAGGCCACCACACCTG
GCTAATGTTTAAATTTTATGTAGAGATGGCGGGGGGGGCGGGGGTCTCCCTATGTTGCCC
AGGCTGGTCTCGAGCTCCTGACCTCAAGTGATCCTCCTGCCCCAGCATTCCAAAGTGCTG
GGATTACAGGTGTGAGCCACTGCCTGGCCTGGCCGTAGGTTTGTAACTGTTTCATAGAAG
AGCCCTGGAGAAGACAGTAGAATGAGCCTATCTAGTTTAAAAAATAAAGAAGCACGTTGA
TTTCAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_133373
Insert Size 3400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_133373.2
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133373.2, NP_588614.1
RefSeq Size 3445 bp
RefSeq ORF 2370 bp
Locus ID 113026
UniProt ID Q8N3E9
Domains C2, PI-PLC-X, PI-PLC-Y, PH
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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