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RAD17 (NM_133338) Human Untagged Clone
CAT＃: SC120501
RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 1
CNY 4,912.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCYC; HRAD17; R24L; RAD17SP; RAD24 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_133338, the custom clone sequence may differ by one or more nucleotides
ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTACTA TTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAAAG CAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAATTCA AAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGCTG TGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAACA GGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTATCA AAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGATGATTTCA AGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTTTTCAAAGA GTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACTGATAAGAAG ATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGA AGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGAGATAATAATCA AAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTCAACCCTGTGGCA CCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAATGGAGGAAAAATTA CTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATCAGAAGTGCAATAAA CAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAAGGAATGTCTTTAAAA TCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTTGAAAATCAAGAGGTCC AAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAAATTCTATATTGTAAAAG AGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAATATGAACGGGATACATTA CTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTATTTAATTTATATCTTCACC AAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGA TATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGCACATCTATAGCTACGAGAGGT GTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGAGGATCAAGTTTTCGACCCTTGC ACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTTCC TGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTCCA ATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGGAA GATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACCCTGACAGCGGAGATGAAGCCCA GCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCAAGCCACTGTGCCGGAAACCTGG TCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAG GAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGTGATGGGACATAG >OriGene 5' read for NM_133338 unedited
GTAGTATATAGGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGACGAA ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGC GTCTCTACTATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAAT GGGCCTTCTACATTAGAAAGCAGCAGATTTCCAGCGTAGAAAAAGAGGAAATCTATCTTC CTTAGAACAGATTTATGGTTTAGAAAATTCAAAAGAATATCTGTCTGAAAATGAACCATG GGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGA AGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAACAGGGTGGATC TATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTATC AAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAA AGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTC TCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAAT GCTTGGAGATGATCTGAGAACTGATAAGAAAGATATTCTGGTTGAAGATTACCTAACCAG TTNTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGAAGTATGTGNAGATTGGTCGA TGTCCTCTTNATATTATAATCTCCGACAGTCTCAGTGGAGATATAATCAAAGGTATTGTT TCCAAAGAAAATCAGAAGATGTCTATCTCAAATATAGTTTCACCCTGTGCACCNACATAT GATGAAATNNCTATCGATATGACTATGAGCTACAGATGGAGAAAATTCTGTCCTGCAAAC T >OriGene 3' read for NM_133338 unedited
NGTATTCTATGNACCGCGCCGCTTTTNANGANCGNATTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTAAATTCAACATTTTAATTGGGATATAACTTATGTAAATACTTTCCA AAAATATCTAAAATATTCCACTGTACAACACATCAAAACTTTTTGAAAAGCGGGAGACAA TAAAGTGTAAATAAGGCACACAGTTTACACTCTTAATTGCATCCTAAACTGTTGAATAGT TGATATTTACAACCCCCCAATAACGTTTTTGCATCCTGAATTAATCCTTCACATCTTGAT ATTTGGCATAATGTTCAAACACACTTTATACACCCTGACCGCTCCTTAAACCACTCACAG ATTTAATAAATCCCGAAAAAATTAAAAAATTTGAAGGCTTGATCTTCTATTTACTTCAAA ATGACAAAAAAGTAGGTTTCTTGTGATGAAATACTCCCAGAATGAAGAATTAACAAACTG TTGTGTAATTCATCAAAAAAGCATATTAACTCTGCTGAAGTACCACTGAATGAAACAAAA ATGAAGCTGTGAAGTACCAATCTGATTAGCAGGCTGGCTTCTATGTCCCATCACTCTCGA AGCCTTCTATTATTATGTTTTCTTCCATGTCTCCTTGGGCTGAAAAGGGCTGGGGCTGGC TAGCAGGCAGTTCACTGGCACTATTCTGACTCAAAGGAAGAGACCAGGTTTCCGGCACAG TGGCTTGAGTGGGTTCACCCAGAGATTCCTCTGCAGAATGTCCTCCATTAAGCTGGGCTT CATCTCCGCTGTCAGGGTCTATCATTCCATGTTCCCTGTCAGTCAGGGCTTCCATTTTCA ATCTTCCAAAGTGTCGCTTCAGAGGAGGCTTCCAATATCTTGGATAAAAGAAATCTGACC TTGAATTCTCATTGGAATGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_133338 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133338.1, NP_579916.1 |
| RefSeq Size | 3076 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 5884 |
| UniProt ID | O75943 |
| Domains | Rad17 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) encodes the predominant isoform (1). Variants 1, 5, 6, 7, 8 and 9 encode the same isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220222 | RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 1 |
CNY 4,912.00 |
|
| RC220222L3 | Lenti-ORF clone of RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 1 |
CNY 5,890.00 |
|
| RC220222L4 | Lenti-ORF clone of RAD17 (mGFP-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 1 |
CNY 5,890.00 |
|
| RG220222 | RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 1 |
CNY 4,370.00 |
