EXT1 (NM_000127) Human Untagged Clone

CAT#: SC120098

EXT1 (untagged)-Human exostosin 1 (EXT1)



  "NM_000127" in other vectors (7)

CNY 5,472.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
EXT1 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EXT; LGCR; LGS; TRPS2; TTV
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC120098 sequence for NM_000127 edited (data generated by NextGen Sequencing)
ATGCAGGCCAAAAAACGCTATTTCATCCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTG
TTTTATTTCGGAGGCTTGCAGTTTAGGGCATCGAGGAGCCACAGCCGGAGAGAAGAACAC
AGCGGTAGGAATGGCTTGCACCACCCCAGTCCGGATCATTTCTGGCCCCGCTTCCCGGAC
GCTCTGCGCCCCTTCGTTCCTTGGGATCAATTGGAAAACGAGGATTCCAGCGTGCACATT
TCCCCCCGGCAGAAGCGAGATGCCAACTCCAGCATCTACAAAGGCAAGAAGTGCCGCATG
GAGTCCTGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTACGTATACCCA
CAGCAAAAAGGGGAGAAAATCGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGC
TCCAGGTTCTACACCTCGGACCCCAGCCAGGCGTGCCTCTTTGTCCTGAGTCTGGATACT
TTAGACAGAGACCAGTTGTCACCTCAGTATGTGCACAATTTGAGATCCAAAGTGCAGAGT
CTCCACTTGTGGAACAATGGTAGGAATCATTTAATTTTTAATTTATATTCCGGCACTTGG
CCTGACTACACCGAGGACGTGGGGTTTGACATCGGCCAGGCGATGCTGGCCAAAGCCAGC
ATCAGTACTGAAAACTTCCGACCCAACTTTGATGTTTCTATTCCCCTCTTTTCTAAGGAT
CATCCCAGGACAGGAGGGGAGAGGGGGTTTTTGAAGTTCAACACCATCCCTCCTCTCAGG
AAGTACATGCTGGTATTCAAGGGGAAGAGGTACCTGACAGGGATAGGATCAGACACCAGG
AATGCCTTATATCACGTCCATAACGGGGAGGACGTTGTGCTCCTCACCACCTGCAAGCAT
GGCAAAGACTGGCAAAAGCACAAGGATTCTCGCTGTGACAGAGACAACACCGAGTATGAG
AAGTATGATTATCGGGAAATGCTGCACAATGCCACTTTCTGTCTGGTTCCTCGTGGTCGC
AGGCTTGGGTCCTTCAGATTCCTGGAGGCTTTGCAGGCTGCCTGTGTCCCTGTGATGCTC
AGCAATGGATGGGAGTTGCCATTCTCTGAAGTGATTAATTGGAACCAAGCTGCCGTCATA
GGCGATGAGAGATTGTTATTACAGATTCCTTCTACAATCAGGTCTATTCATCAGGATAAA
ATCCTAGCACTTAGACAGCAGACACAATTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAG
AAGATTGTATTAACTACACTAGAGATTATTCAGGACAGAATATTCAAGCACATATCACGT
AACAGTTTAATATGGAACAAACATCCTGGAGGATTGTTCGTACTACCACAGTATTCATCT
TATCTGGGAGATTTTCCTTACTACTATGCTAATTTAGGTTTAAAGCCCCCCTCCAAATTC
ACTGCAGTCATCCATGCGGTGACCCCCCTGGTCTCTCAGTCCCAGCCAGTGTTGAAGCTT
CTCGTGGCTGCAGCCAAGTCCCAGTACTGTGCCCAGATCATAGTTCTATGGAATTGTGAC
AAGCCCCTACCAGCCAAACACCGCTGGCCTGCCACTGCTGTGCCTGTCGTCGTCATTGAA
GGAGAGAGCAAGGTTATGAGCAGCCGTTTTCTGCCCTACGACAACATCATCACAGACGCC
GTGCTCAGCCTTGACGAGGACACGGTGCTTTCAACAACAGAGGTGGATTTCGCCTTCACA
GTGTGGCAGAGCTTCCCTGAGAGGATTGTGGGGTACCCCGCGCGCAGCCACTTCTGGGAT
AACTCTAAGGAGCGGTGGGGATACACATCAAAGTGGACGAACGACTACTCCATGGTGTTG
ACAGGAGCTGCTATTTACCACAAATATTATCACTACCTATACTCCCATTACCTGCCAGCC
AGCCTGAAGAACATGGTGGACCAATTGGCCAATTGTGAGGACATTCTCATGAACTTCCTG
GTGTCTGCTGTGACAAAATTGCCTCCAATCAAAGTGACCCAGAAGAAGCAGTATAAGGAG
ACAATGATGGGACAGACTTCTCGGGCTTCCCGTTGGGCTGACCCTGACCACTTTGCCCAG
CGACAGAGCTGCATGAATACGTTTGCCAGCTGGTTTGGCTACATGCCGCTGATCCACTCT
CAGATGAGGCTCGACCCCGTCCTCTTTAAAGACCAGGTCTCTATTTTGAGGAAGAAATAC
CGAGACATTGAGCGACTTTGA

Clone variation with respect to NM_000127.2
1065 c=>t
>OriGene 5' read for NM_000127 unedited
GGGTCANNATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCACTAGCC
GGCTGAACCCGGGATCGGCTGACTTGTTGGAACCGGAGTGCTCTGCACGGAGAGTGGTGG
ATGAGTTGAAGTTGCCTTCCCGGGGCTCATTTTCCACGCTGCCGAGAGGAATCCGAGAGG
CAAGGCAATCACTTCGTCTTGCCATTGATTGGGTATCGGGAGCTTTTTTTTTCTCCTCTC
TCTCTTTCTTTTCCTCCGTCTTGTTGCATGCAAGAAAATTACAGTCCGCTGCTCGCCCGC
CCTGGGTGCGAGATATTCAGCCCCGCTCTCTCCCGTGCATTGTGCAACCCAAAGATGAAA
GACCGAAGGGGAGAAAGTTAAAGAAATCGCCCACATGCGCTGGATCAGTCCACGGCTTGG
GGAAAGGCATCCAGAGAAGGTGGGAGCGGAGAGTTTGAAGTCTTTACAGGCGGGAAGATG
GCGGACTGGAGCTGAAAGTGTTGATTGGGAAACTTGGGTGATTCTTGTGTTTATTTACAA
TCCTCTTGACCCAGGCAGGACACATGCAGGCCAAAAAACGCTATTTCATCCTGCTCTCAG
CTGGCTCTTGTCTCGCCCTTTTGTTTTATTTCGGAGGCTTGCAGTTTAAGGCATCGAGGA
GCCACAGCCGGAGAGAAGAACACAGCGGTAGGAATGGCTTGCACCACCCCAGTCCGGATC
ATTTCTGGCCCCGCTTCCCGGACGCTCTGCGCCCCTTCGTTCCTTGGGATCAATTGGAAA
ACGAGGATTCCAGCGTGCACATTTCCCCCCGGCAGAAGCGAGATGCCAACTCCAGCATCT
ACAAAGGGCAGAAGTGCCGCATGGAGTCCTGCTTCGATTTCACCTTTTGCAGAAANNACG
CTCAAGTCTACGTATACCCACAGCAAAGGGGAAG
>OriGene 3' read for NM_000127 unedited
NNGGTCNTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAA
CCCCCCCAATGAAAAACGATTATCATATATATCTATCTCAAACATAGAGATTTCAGTTAG
AACTGCCTACCTGGAAGTCGTAATACTTTCTTTCATGCAGCGACATTTTAGAGATAAAAG
TTCAAGTGGATTTGAAGATTCGAAGTGGAAAACAATTTGCACTGGCAAAGTTGAATAATG
AAGTTACAACTTATGCTCAGTTATTTCTTGATAATGGGTAGAGTGACTTATTGTGTTTTC
CACGAAGTTTGAGCTTTTGAAACTGCAGGGCACCCTACATGGCCATGACAATGATGTCTG
TGGGAAAAGGAATAGCAGCTTTGAAATATTCACAACCAACACAATTTTGATTAAACAAAG
AACTCTGGTTTTTAATAGTTTTGATCATTAAAAAAGTTTAAACCTGCATAGCAATCATTT
CAAAAATAATTATTTAATGTTCCATAATTAAACTGTACACAACCTAGTCTTGGGACACAG
AAGCCAGTGAGGTGAGTTTGGAGCAGTCTGGTGCAGTCCCAGGAGCCAGGAGTTGAGTTC
TCATTGGCCTTTTTTTTTTTGTCATTCTGCTCATCTAAGTTTTTGGATAGTTGGCACAAT
CTGGCTCTGCTGATGAGTGGATCTGCACTGGGAAGAGAGAGCAGCTTGACCCCCATCCCT
TCTTGCTTTCCCTCCCCACTCAGCCGGATCCTCAAGTCGCTCAATGTCTCGGTATTTCTT
CCTCAAATANAACCTGGTCTTAAAGAGGACCGGGGCGAGCCTTATCTGAAATGGATCAGC
GGCTGTACCCAACCAGCTGGCAACGTTTTATGCAACTTGGCCCTGGGCAAGGGCCAGGGT
CANCCCACGGGAACCCCGAAAGCTGTCCATCTTG
Restriction Sites NotI-NotI     
ACCN NM_000127
Insert Size 3460 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000127.2, NP_000118.2
RefSeq Size 3376 bp
RefSeq ORF 2241 bp
Locus ID 2131
UniProt ID Q16394
Domains Exostosin
Protein Families Druggable Genome, Transmembrane
Protein Pathways Heparan sulfate biosynthesis, Metabolic pathways
Gene Summary This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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