Nucleoside phosphorylase (PNP) (NM_000270) Human Untagged Clone
CAT#: SC120008
PNP (untagged)-Human purine nucleoside phosphorylase (PNP)
CNY 2,400.00
CNY 6,270.00
Product images
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | NP; PRO1837; PUNP |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC120008 sequence for NM_000270 edited (data generated by NextGen Sequencing)
ATGGAGAACGGATACACCTATGAAGATTATAAGAACACTGCAGAATGGCTTCTGTCTCAC ACTAAGCACCGACCTCAAGTTGCAATAATCTGTGGTTCTGGATTAGGAGGTCTGACTGAT AAATTAACTCAGGCCCAGATCTTTGACTACGGTGAAATCCCCAACTTTCCCCGAAGTACA GTGCCAGGTCATGCTGGCCGACTGGTGTTTGGGTTCCTGAATGGCAGGGCCTGTGTGATG ATGCAGGGCAGGTTCCACATGTATGAAGGGTACCCACTCTGGAAGGTGACATTCCCAGTG AGGGTTTTCCACCTTCTGGGTGTGGACACCCTGGTAGTCACCAATGCAGCAGGAGGGCTG AACCCCAAGTTTGAGGTTGGAGATATCATGCTGATCCGTGACCATATCAACCTACCTGGT TTCAGTGGTCAGAACCCTCTCAGAGGGCCCAATGATGAAAGGTTTGGAGATCGTTTCCCT GCCATGTCTGATGCCTACGACCGGACTATGAGGCAGAGGGCTCTCAGTACCTGGAAACAA ATGGGGGAGCAACGTGAGCTACAGGAAGGCACCTATGTGATGGTGGCAGGCCCCAGCTTT GAGACTGTGGCAGAATGTCGTGTGCTGCAGAAGCTGGGAGCAGACGCTGTTGGCATGAGT ACAGTACCAGAAGTTATCGTTGCACGGCACTGTGGACTTCGAGTCTTTGGCTTCTCACTC ATCACTAACAAGGTCATCATGGATTATGAAAGCCTGGAGAAGGCCAACCATGAAGAAGTC TTAGCAGCTGGCAAACAAGCTGCACAGAAATTGGAACAGTTTGTCTCCATTCTTATGGCC AGCATTCCACTCCCTGACAAAGCCAGTTGA Clone variation with respect to NM_000270.3 >OriGene 5' read for NM_000270 unedited
TTGTAACACGACTTTACTATANNGGCGGCCGCGNAATTCGGCACGAGGGACCCGGCAGCC TTGCTCAGTTCAGCATAGCGGAGCGGATCCGATCGGATCGGAGCGGATCGGAGCACACCG GAGCAGGCTCATCGAGAAGGCGTCTGCGAGACCATGGAGAACGGATACACCTATGAAGAT TATAAGAACACTGCAGAATGGCTTCTGTCTCACACTAAGCACCGACCTCAAGTTGCAATA ATCTGTGGTTCTGGATTAGGAGGTCTGACTGATAAATTAACTCAGGCCCAGATCTTTGAC TACGGTGAAATCCCCAACTTTCCCCGAAGTACAGTGCCAGGTCATGCTGGCCGACTGGTG TTTGGGTTCCTGAATGGCAGGGCCTGTGTGATGATGCAGGGCAGGTTCCACATGTATGAA GGGTACCCACTCTGGAAGGTGACATTCCCAGTGAGGGTTTTCCACCTTCTGGGTGTGGAC ACCCTGGTAGTCACCAATGCAGCAGGAGGGCTGAACCCCAAGTTTGAGGTTGGAGATATC ATGCTGATCCGTGACCATATCAACCTACCTGGTTTCAGTGGTCAGAACCCTCTCAGAGGG CCCAATGATGAAAGGTTTGGAGATCGTTTCCCTGCCATGTCTGATGCCTACGACCGGACT ATGAGGCAGAGGGCTCTCAGTACCTGGAAACAAATGGGGGAGCAACGTGAGCTACANGGA AGCACCTATGTGATGGTGGCAGGCCCCAGCTTTGAGACTGTGGCAGAATGTCGTGTGCTG CAAAAGCTGGGAGCAGACGCTGTTGGCATGAGTACAGTACCAGAAGTTATCCGTGCACGG CACTGTGGACCTCCAGTCTTTGGCCNCTCACTCATCACTAACAAGGGCTCATGGAT >OriGene 3' read for NM_000270 unedited
TTTTATGCCACGCGGCCCGCAATCTAATATCGAGTTTTTTTTTTTTTTTTTTATTTCATC TTTCTCTTTATCCTGACAACATTATGACATTCAATAAAATCCAAGAGGTATTGGATTACC CCCTTGCTTGGTCCCTGGTGATTTACATCCGGTACAACTGATCCTCTCCGAACGTATTAT TTCTAAGAGCTAGCACCAGCTGCTATGCAAAGCTCTAAGGGCCGAGCCAAATCCTGGGCA TCTCCACAGCATGTGCGGCAGGGCACGCGCCTTAGTTCTCGTGTAAGAAAGACCAGGAAC GAGAACAGCTTTATTCCGTACCGCTAATGTTCTTCTATGCTCACACTTGTTCTTCTAGCT TTGAGACAAGGACCCGGCCCCCCCTCGTCTTGCCATAAGACCGTGGGAAAGGCGAACGCA ATGACACCTCTCCTTTCTGCTCCACCTATTCCCCCACGCCTCGCACTCTTCTTCGCGCCC TAGACCGCCGCACCTTCCCGGTTCCCTGCGTGTATACGCCTCACTATCCCTTCGTTTCCC TTTTGATCTCCCATGCCGGCCACCCCCTCCCCACACGTGCACCCTTTGTCCCCATCCTGT CGCTCTGTTCCGCCATCGTCATACTTCTCCTGTTTGACCTTCCCCTCGTCCCCCCTCCTT TTCCATCTTCTTTCCCCCCTTACTTCCCCCACTTTCCCTTCTCCCGCCCTCCATACTCCT CCGTCCCCCTTCCTTTCCCCCCTTCCCCCCCTTTTTCCGCCTCCTTTTTTCCCCCCCTTT TTTCCCCGCGTTCCGCTTCCTCTCTTCCCCCTTTTTAACTCCCCCCTCTTTTTTCTTTCC ATACAGAGTATCCCTCCTCCTCCCCGTTCTTCCCTCTATTTCCGACTACTATTCCCCCCC CATCTCT |
Restriction Sites | NotI-NotI |
ACCN | NM_000270 |
Insert Size | 1530 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000270.1, NP_000261.1 |
RefSeq Size | 1418 bp |
RefSeq ORF | 870 bp |
Locus ID | 4860 |
UniProt ID | P00491 |
Domains | Mtap_PNP |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
Gene Summary | This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210759 | PNP (Myc-DDK-tagged)-Human purine nucleoside phosphorylase (PNP) |
CNY 2,400.00 |
|
RC210759L3 | Lenti ORF clone of Human purine nucleoside phosphorylase (PNP), Myc-DDK-tagged |
CNY 5,890.00 |
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RC210759L4 | Lenti ORF clone of Human purine nucleoside phosphorylase (PNP), mGFP tagged |
CNY 5,890.00 |
|
RG210759 | PNP (tGFP-tagged) - Human purine nucleoside phosphorylase (PNP) |
CNY 4,000.00 |
|
SC322018 | PNP (untagged)-Human purine nucleoside phosphorylase (PNP) |
CNY 2,400.00 |
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