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MLH1 (NM_000249) Human Untagged Clone
CAT＃: SC119993
MLH1 (untagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1
CNY 5,544.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119993 sequence for NM_000249 edited (data generated by NextGen Sequencing)
ATGTCGTTCGTGGCAGGGGTTATTCGGCGGCTGGACGAGACAGTGGTGAACCGCATCGCG GCGGGGGAAGTTATCCAGCGGCCAGCTAATGCTATCAAAGAGATGATTGAGAACTGTTTA GATGCAAAATCCACAAGTATTCAAGTGATTGTTAAAGAGGGAGGCCTGAAGTTGATTCAG ATCCAAGACAATGGCACCGGGATCAGGAAAGAAGATCTGGATATTGTATGTGAAAGGTTC ACTACTAGTAAACTGCAGTCCTTTGAGGATTTAGCCAGTATTTCTACCTATGGCTTTCGA GGTGAGGCTTTGGCCAGCATAAGCCATGTGGCTCATGTTACTATTACAACGAAAACAGCT GATGGAAAGTGTGCATACAGAGCAAGTTACTCAGATGGAAAACTGAAAGCCCCTCCTAAA CCATGTGCTGGCAATCAAGGGACCCAGATCACGGTGGAGGACCTTTTTTACAACATAGCC ACGAGGAGAAAAGCTTTAAAAAATCCAAGTGAAGAATATGGGAAAATTTTGGAAGTTGTT GGCAGGTATTCAGTACACAATGCAGGCATTAGTTTCTCAGTTAAAAAACAAGGAGAGACA GTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGACAATATTCGCTCCGTCTTT GGAAATGCTGTTAGTCGAGAACTGATAGAAATTGGATGTGAGGATAAAACCCTAGCCTTC AAAATGAATGGTTACATATCCAATGCAAACTACTCAGTGAAGAAGTGCATCTTCTTACTC TTCATCAACCATCGTCTGGTAGAATCAACTTCCTTGAGAAAAGCCATAGAAACAGTGTAT GCAGCCTATTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTAGAAATCAGTCCC CAGAATGTGGATGTTAATGTGCACCCCACAAAGCATGAAGTTCACTTCCTGCACGAGGAG AGCATCCTGGAGCGGGTGCAGCAGCACATCGAGAGCAAGCTCCTGGGCTCCAATTCCTCC AGGATGTACTTCACCCAGACTTTGCTACCAGGACTTGCTGGCCCCTCTGGGGAGATGGTT AAATCCACAACAAGTCTGACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCC CACCAGATGGTTCGTACAGATTCCCGGGAACAGAAGCTTGATGCATTTCTGCAGCCTCTG AGCAAACCCCTGTCCAGTCAGCCCCAGGCCATTGTCACAGAGGATAAGACAGATATTTCT AGTGGCAGGGCTAGGCAGCAAGATGAGGAGATGCTTGAACTCCCAGCCCCTGCTGAAGTG GCTGCCAAAAATCAGAGCTTGGAGGGGGATACAACAAAGGGGACTTCAGAAATGTCAGAG AAGAGAGGACCTACTTCCAGCAACCCCAGAAAGAGACATCGGGAAGATTCTGATGTGGAA ATGGTGGAAGATGATTCCCGAAAGGAAATGACTGCAGCTTGTACCCCCCGGAGAAGGATC ATTAACCTCACTAGTGTTTTGAGTCTCCAGGAAGAAATTAATGAGCAGGGACATGAGGTT CTCCGGGAGATGTTGCATAACCACTCCTTCGTGGGCTGTGTGAATCCTCAGTGGGCCTTG GCACAGCATCAAACCAAGTTATACCTTCTCAACACCACCAAGCTTAGTGAAGAACTGTTC TACCAGATACTCATTTATGATTTTGCCAATTTTGGTGTTCTCAGGTTATCGGAGCCAGCA CCGCTCTTTGACCTTGCCATGCTTGCCTTAGATAGTCCAGAGAGTGGCTGGACAGAGGAA GATGGTCCCAAAGAAGGACTTGCTGAATACATTGTTGAGTTTCTGAAGAAGAAGGCTGAG ATGCTTGCAGACTATTTCTCTTTGGAAATTGATGAGGAAGGGAACCTGATTGGATTACCC CTTCTGATTGACAACTATGTGCCCCCTTTGGAGGGACTGCCTATCTTCATTCTTCGACTA GCCACTGAGGTGAATTGGGACGAAGAAAAGGAATGTTTTGAAAGCCTCAGTAAAGAATGC GCTATGTTCTATTCCATCCGGAAGCAGTACATATCTGAGGAGTCGACCCTCTCAGGCCAG CAGAGTGAAGTGCCTGGCTCCATTCCAAACTCCTGGAAGTGGACTGTGGAACACATTGTC TATAAAGCCTTGCGCTCACACATTCTGCCTCCTAAACATTTCACAGAAGATGGAAATATC CTGCAGCTTGCTAACCTGCCTGATCTATACAAAGTCTTTGAGAGGTGTTAA Clone variation with respect to NM_000249.3 655 a=>g >OriGene 5' read for NM_000249 unedited
GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCAAATGTCGTTCGTGGCAGGG GTTATTCGGCGGCTGGACGAGACAGTGGTGAACCGCATCGCGGCGGGGGAAGTTATCCAG CGGCCAGCTAATGCTATCAAAGAGATGATTGAGAACTGTTTAGATGCAAAATCCACAAGT ATTCAAGTGATTGTTAAAGAGGGAGGCCTGAAGTTGATTCAGATCCAAGACAATGGCACC GGGATCAGGAAAGAAGATCTGGATATTGTATGTGAAAGGTTCACTACTAGTAAACTGCAG TCCTTTGAGGATTTAGCCAGTATTTCTACCTATGGCTTTCGAGGTGAGGCTTTGGCCAGC ATAAGCCATGTGGCTCATGTTACTATTACAACGAAAACAGCTGATGGAAAGTGTGCATAC AGAGCAAGTTACTCAGATGGAAAACTGAAAGCCCCTCCTAAACCATGTGCTGGCAATCAA GGGACCCAGATCACGGTGGAGGACCTTTTTTACAACATAGCCACGAGGAGAAAAGCTNTA AAAAATCCAAGTGAAGAATATGGGAAAATTTTGGAAGTTGTTGGCAGGTATTCAGTACAC AATGCANGCATTAGTTTCTCAGTTAANAAACAAGGAGAGACAGTAGCTGATGTTANGACA CTACCNCATGCCTNCACCGTGGGACATATTCGCTCCGTCTTTGGAAATGCTGNTAGTCGA GAACTGATAGAAATGGNATGTGANNGATAAACCCNTAGCCTTCAAATGAATGGNTACATA TCCATGCANACTACTCAGTGAAGAAGTGCATCTTTCTACTCTTCATCACCATCGTCTGGT AGAATCACTTNCTTGAAAAAGCATAGAACAGGTATGCAGCCTATTGCCAAAACACCACCC ATTCTGTACTCATTAGAATCATCCCAGATGTGATGTATGTGCCCCACAAGCTGAGTCACT TCTGACAGAGACTCTGGACGGTG >OriGene 3' read for NM_000249 unedited
ACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTAAGACACATCTATTTATTT ATAATCAATCCACTGTGTATAAAGGAATACTATCAGAAGGCAAGTATAAGTCTTAAGTGC TACCAACACTTATGTTGGTACACTTTGTATATCACACTTTGATACAACACTTTGTATCGG AATACAGAGAAAGAAGAACACATCCCACAGTGCATAAATAACCATATTTAACACCTCTCA AAGACTTTGTATAGATCAGGCAGGTTAGCAAGCTGCAGGATATTTCCATCTTCTGTGAAA TGTTTAAGAGGCAGAATGTGTGAGCGCAAGGCTTTATAGACAATGTGTTCCACAGTCCAC TTCCAGGAGTTTGGAATGGAGCCAGGCACTTCACTCTGCTGGCCTGAGAGGGTCGACTCC TCAGATATGTACTGCTTCCGGATGGAATAGAACATAGCGCATTCTTTACTGAGGCTTTCA AAACATTCCTTTTCTTCGTCCCAATTCACCTCAGTGGCTAGTCGAAGAATGAATATAGGC AGTCCCTCCAAAGGGGGCACATAGTTGTCAATCAGAAGGGGTAATCCAATCAGGTTCCCT TCCTCATCAATTTCCAAAGAAGAATAGTCTGCAAGCCTCCCAACCTTCTTCTTCAGAAAC TAACAATGTATTCACCAAGTCCTTCTTTTGGACCATCTTCCTCTGGCCAGCCACTCCTTG GATTTTTAAGGCACCCTGGCAGGGCAAAAAGCGAGCTGGCTCCGTTACCCGAGAACCCAA ATCGGGAAATCCTAATGACCTCGGCACAAACCCCTCCCCACCTGGGGGGGTGAAAAGAAT ACTGGTTCAGCTGTGCCAGGCCCCTGGTTCACCTCCCCGAGGTGGGTTTACACTTTTCGA AAACTAGTACCCGCCATTCTTTCCCGGCACTCTCCCCTCGGGCTTTGTCTCCCGTGGGCG CATCTT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_000249 |
| Insert Size | 2600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000249.2, NP_000240.1 |
| RefSeq Size | 2524 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 4292 |
| UniProt ID | P40692 |
| Domains | DNA_mis_repair, HATPase_c |
| Protein Families | Druggable Genome |
| Protein Pathways | Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer |
| Gene Summary | The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201607 | MLH1 (Myc-DDK-tagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1 |
CNY 5,536.00 |
|
| RC201607L1 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, Myc-DDK-tagged |
CNY 7,936.00 |
|
| RC201607L2 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC201607L3 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201607L4 | Lenti ORF clone of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, mGFP tagged |
CNY 7,936.00 |
|
| RG201607 | MLH1 (tGFP-tagged) - Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1 |
CNY 7,136.00 |
|
| SC323792 | MLH1 (untagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1 |
CNY 5,544.00 |
