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SRD5A2 (NM_000348) Human Untagged Clone
CAT＃: SC119922
SRD5A2 (untagged)-Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2)
CNY 2,400.00
CNY 3,990.00
| Cited in 7 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000348 edited
CGGCACGAGGCAGCGGCCACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAG AGCCCAGTGCTGGCAGGCAGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTC GCGAAGCCCTCCGGCTACGGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTG CCAGCCCGCGCCGCCTGGTTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATC CTCGCCCGGCAGCCCCTCTCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTC TGCCTACATTACTTCCACAGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTAT CCAGCTATACTCATTCTCAGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGC TACTATCTGATTTACTGTGCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGC TTGGGTGTCTTCTTATTTATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTG CGCCAGCTCAGGAAGCCTGGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACG TATGTTTCTGGAGCCAATTTCCTCGGTGAGATCATTGAATGGATCGGCTATGCCCTGGCC ACTTGGTCCCTCCCAGCACTTGCATTTGCATTTTTCTCACTTTGTTTCCTTGGGCTGCGA GCTTTTCACCACCATAGGTTCTACCTCAAGATGTTTGAGGACTACCCCAAATCTCGGAAA GCCCTTATTCCATTCATCTTTTAAAGGAACCAAATTAAAAAGGAGCAGAGCTCCCACAAT GCTGATGAAAACTGTCAAGCTGCTGAAACTGTAATTTTCATGATATAATAGTCCCGTATA TATGTAATAGTAGGTCTCCTGGCGTTCTGCCAGCTGGCCTGGGGATTCTGAGTGGTGTCT GCTTAGAGTTTACTCCTACCCTTCCAGGGACCCCTATCCTGATCCCCAACTGAAGCTTCA AAAAGCCACTTTTCCAAATGGCGACAGTTGCTTCTTAGCTATTGCTCTGAGAAAGTACAA ACTTCTCCTATGTCTTTCACCGGGCAATCCAAGTACATGTGGCTTCATACCCACTCCCTG TCAATGCAA >OriGene 5' read for NM_000348 unedited
TCGCATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCAGCGGCC ACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAGAGCCCAGTGCTGGCAGGC AGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTCGCGAAGCCCTCCGGCTAC GGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTGCCAGCCCGCGCCGCCTGG TTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATCCTCGCCCGGCAGCCCCTC TCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTCTGCCTACATTACTTCCAC AGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTATCCAGCTATACTCATTCTC AGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGCTACTATCTGATTTACTGT GCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGCTTGGGTGTCTTCTTATTT ATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTGCGCCAGCTCAGGAAGCCT GGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACGTATGTTTCTGGAGCCAAT TTCCTCGGTGAGATCATTGAATGGNATCGCTATGCCCTGGCCACNTGGTCCCTCCNAGCA CTTGCATTTGCATNTTTCTCACTTTTGTTTCTTGGGCTGCGAGCTTTTCACCACCATAAG TTCTACCTCAAGATGTTGAGGGACTACCCAATCTCGAAAGCCCCTATNCATTCATCTTTA AGGAACCANTTAAAAAGAGCAGACTCCNACATGCTGATGAAACTGTCAGCTGCTGAACTG GNATTTCATGAATATAGTCCGTTATATGTAAC >OriGene 3' read for NM_000348 unedited
GCCGCAATCTAGAGTCGAGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGGA TTAAAAGGTTTTTAATGAACAACAAAAACACTTATTTATATGATGGCAATTGGCATTTTT CAGTAATCTACATCATCCTAAAACCTTTAAAGTTTCCTATGGGACTTATATAGGGAGTTT CTGGGCTTAGTCCCACTGGGGCTCATTTGTCAAAACAGTTGGCTAAATATTGACAATGCA TTCCGCAAACATAGGCCTGAAAAAACAAATATTTCCATGTATTCCAATTACAAGCGTTCG GCCCCTTCCTTAAAGAGTCCATATCTCAGCACTCATGCTGGGGGGACCCCTTCACAAAAG TTGGCAAACTCAAAGGCTTACTACCTACGTAGTTCCAGTTCTGGGGTGGAAATGTGGGCT CTGTATGTTCAAATCCTCCAACTTTTTAAAGGAACCTAAAATGCTAGAGTTTTATGAAGG AATCTCCTGATTTTAAAGTTTTTGAACCTTTGGAAAAAAAAAAAAACCTTTTGGTCTGAC CCAAACCAACCAGGTTTTTTGCCAAAACGTGCCCCTTTGTGTGCAATTTTCAGCCTCTGC CTGCAGGTCCTTTGAGGAAGGCATTCAGGCCGCCCTTAGGAAACACCTTCTTGACACGGC CCGAAAATTCAAGGAAAACCTCCTTAAAGGAAATTTTTTTGGGGCTCTCGCGTCCCTCAT TTGGCGGGGACCTCGGGGGGGGGCAACACCCCTGCCACCCTATCATATACCCTCCCGCCC TACCCCCACCATTTTCGGCCTCCGCCCCCCCCCCCCACACACCTACGTTCCCCCCGCCTT TGCAAATTGTTTCCTTCCCCGCGGACCCCCACAGCCATTCGCGTCCCTCCCTTTTCCACA CACCCCACGTTAGTCCTTTTTGTCTCCGCGTCCATATATTGCCCGTCCAACTATCCGTCA CTATTATTGGCCTCCGGTCCCCCCCTGATTACATATTCTCTTATCACAACCTGCGTCGCC CTCACGAGGGTCGTTGTTGTCGCTTAGCCAACTCCATTTAGGTGCTACTGATAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000348 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000348.2, NP_000339.2 |
| RefSeq Size | 2464 bp |
| RefSeq ORF | 765 bp |
| Locus ID | 6716 |
| UniProt ID | P31213 |
| Domains | Steroid_dh |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Androgen and estrogen metabolism, Prostate cancer |
| Gene Summary | This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mutational analysis of SRD5A2: From gene to functional kinetics in individuals with steroid 5α-reductase 2 deficiency
,Ramos, L;Vilchis, F;Chávez, B;Mares, L;,
J. Steroid Biochem. Mol. Biol.
,PubMed ID 32380235
[SRD5A2]
|
|
Hamster SRD5A3 lacks steroid 5a-reductase activity in vitro
,Chávez, B;Ramos, L;García-Becerra, R;Vilchis, F;,
Steroids
,PubMed ID 25498908
[SRD5A2]
|
|
46,XY disorder of sexual development resulting from a novel monoallelic mutation (p.Ser31Phe) in the steroid 5α-reductase type-2 (SRD5A2) gene
,null,
Molecular Genetics & Genomic Medicine
,PubMed ID 25077171
[SRD5A2]
|
|
46, XY disorder of sexual development resulting from a novel monoallelic mutation (p. Ser31Phe) in the steroid 5a-reductase type-2 (SRD5A2) gene
,Chávez, B;Ramos, L;Gómez, R;,
Molecular Genetics & Genomic Medicine
[SRD5A2]
|
|
Molecular analysis of the SRD5A2 in 46,XY subjects with incomplete virilization: the P212R substitution of the steroid 5alpha-reductase 2 may constitute an ancestral founder mutation in Mexican patients.
,null,
Journal of andrology
,PubMed ID 20019388
[SRD5A2]
|
|
Molecular Analysis of the SRD5A2 in 46,XY Subjects with Incomplete Virilization: the P212R substitution of the Steroid 5{alpha}-Reductase-2 May Constitute an Ancestral Founder Mutation in Mexican Patients
,Felipe Vilchis, Luis Ramos, Juan Pablo Mendez, Socorro Benavides, Patricia Canto, and Betha Chavez,
J Androl, Dec 2009; 10.2164/jandrol.109.009407
[SRD5A2]
|
|
Novel compound heterozygous mutations in the SRD5A2 gene from 46,XY infants with ambiguous external genitalia.
,null,
Journal of human genetics
,PubMed ID 18350250
[SRD5A2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213025 | SRD5A2 (Myc-DDK-tagged)-Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2) |
CNY 2,400.00 |
|
| RC213025L1 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC213025L2 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), mGFP tagged |
CNY 5,890.00 |
|
| RC213025L3 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213025L4 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), mGFP tagged |
CNY 5,890.00 |
|
| RG213025 | SRD5A2 (tGFP-tagged) - Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2) |
CNY 4,000.00 |
