HLCS (NM_000411) Human Untagged Clone

Name: HLCS (NM_000411) Human Untagged Clone
SKU: SC119915
Price: 5320.00

CAT＃: SC119915

HLCS (untagged)-Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS)

Datasheet

SDS

"NM_000411" in other vectors (6)

CNY 5,320.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

TA504422

HLCS mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC119915 sequence for NM_000411 edited (data generated by NextGen Sequencing) ATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCAC TTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATC CTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCTGAGCAGGACGGTATGGAGCAT GTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCC TCTGGGAGTGAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCAC CTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAGAACAGCACCATTGAGTCAGTC AAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAG AGTGTTGCTGATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAG GCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAGGAAGCCCTCGGCCGGTTCCAC GAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTG CTGGAGGACAGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCT ACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTCATGGCCTATCTTTCTCAGGGA GGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAG GGTGCACTGCACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGYGAGGTG AAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAAGGCCCCGTCCGGCTCAGCCCC GGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCT TTTGGAACTCGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGC TCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTCAAGTCAAGCAATTTTAGAAGA TACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTT CCTGCCTTAACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATG CAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATAAAATCCGGCCAGCTCTCTCTT AGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTG ACCAACATGGAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTG CAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAAGTGACCCCCACAACGATGCGT CTCCTGGATGGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCC CGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTGGCTGAGCCCTGTGGGATGTGCT CTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTT GTCCAGCATCTGATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAG GATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTACAGTGACCTCATGAAGATC GGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGT GGATTTAATGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATAC AATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGATTATCTCATCGCCAGAGTC GTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTT CCCCTTTATTACCGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAG GGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGGCTTCCTCCAGGTTCACCAGGAG GGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTC ATCCTCCCCAAACGGCGGTAA Clone variation with respect to NM_000411.6 834 c=>y 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_000411 unedited GTTCACATTCGGCACCAGGACGGGGCTGGGGCGCGGGCAGGATTTGGGGCTGCGCCGAGG GCGTCCCGACCTGGCCCTTTGCCACGGTAGGTTCCGGCTCCCATGCCTCCGCTGCCATCT GCGCTGTCCTTGGCGACTACGCCTTCACCCCCCTGGGTGCGTTTCAGCGGTCCCGGAGCC TGGCGGGAGCGGGGCCAGGAGCGCTCCGCGGCCGGTATCAGGCCTGCAGTCCATTGAAGA CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGC ATTTGTGACGGAGAGCATTTGGGTACAAAGTGAGAATTTACAGAGATCATCCTCTTCAGA AACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGAT CCCTTTTAGTGCTCCCAGTAACCTTCCAAGATTGTCAAGTGGTCAGACTGTTGTTTGCCA TTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCC CAAAAGATTGTGTCGGTGCACTTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTC TCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGACCCC AAACCAAGGAGAGCAGTGCCTCTGGGAGTGACCTGCTGGGACAGTGACAGGGAAGGGGGC CCCGTTGAGCATATCACCCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_000411 unedited GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACAAATGAATTGGAG GAAAAGAAAATTCACCTACAACTCTAAATTAGATTTCCAGATGCATGGGCACGGACAGGC AGCCGCGTCTCGGGGACGCCCGGCATTACCGCCGTTTGGGGAGGATGAGGTTTCTCAGCA TGTCGAAGGAGTTGCCGTCCGGGTGCACAGTCACAACCTCGCCGCCCTCCTGGTGAACCT GGAGGAAGCCAGAATCGTCCAGGCCAACGATGGACACCTTTGGTCCCTCTGCGCTGCCCA GATGGACTTGCTGACCACTGTGGACCCAGTATCGGTAATAAAGGGGAAGGACGCTGTTGG GCCCTTTGTCCTGAAACTCTTTGATCAGTTTCTCCAGCACAGTCACGACTCTGGCGATGA GATAATCGGCTCTTAAGGGCTTCAGTTCTGCCTTGTGTTGTTTATTGTATTCTGTGATGA GGTCGTTGATGCAAATGGTAGGGTTACTGTTAGTCACATTAAATCCACAGCCAATAAGTA TATAAAATGTTTCTCCCATGAGTGTTGAGTTAACCAGAACTCCGCCGATCTTCATGAGGT CACTGGTATAAATATCGTTGGGCCACTTCACTCGTAAGTTGATATCCTGATACTNCGGAA TGGACCTCACTGCTTCCACGACAGCCACGGGACATCAGATGCTGGACAAACGGGATCCTC TGTCCCAGCTGGGATCTCATTGGGATGGAGATGAGCANAGTNAAAAGAGCCATTCCACAG GGCTTAACCACCATTCCCTCCCCGTCCTTTTGCCTCGGTCTGCCGGGCCGGGATCCTTAT TAAACCATTTCTGCGGGGTTTGAAACATCACCCATCCCAGAACCCTTCTTGGGGGGGTAC TTTGGAAAAAATATACTTTCCCACTGCTGGGCTGCATATTA
Restriction Sites	NotI-NotI
ACCN	NM_000411
Insert Size	3100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_000411.4, NP_000402.3
RefSeq Size	5991 bp
RefSeq ORF	2181 bp
Locus ID	3141
UniProt ID	P50747
Domains	BPL_C, BPL_LipA_LipB
Protein Pathways	Biotin metabolism, Metabolic pathways
Gene Summary	This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011] Transcript Variant: This variant (1) is the shortest transcript. Variants 1, 2 and 3 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Distinct Amino Termini of Two Human HCS Isoforms Influence Biotin Acceptor Substrate Recognition ,Maria Ingaramo and Dorothy Beckett, J. Biol. Chem., Nov 2009; 284: 30862 - 30870 [HLCS]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC209197	HLCS (Myc-DDK-tagged)-Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,320.00
RC209197L1	Lenti ORF clone of Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,720.00
RC209197L2	Lenti ORF clone of Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC209197L3	Lenti ORF clone of Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC209197L4	Lenti ORF clone of Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG209197	HLCS (tGFP-tagged) - Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,920.00

其它HLCS产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies