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PC1/3 (PCSK1) (NM_000439) Human Untagged Clone
CAT＃: SC119892
PCSK1 (untagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1
CNY 8,280.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BMIQ12; NEC1; PC1; PC3; SPC3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119892 sequence for NM_000439 edited (data generated by NextGen Sequencing)
ATGGAGCGAAGAGCCTGGAGTCTGCAGTGCACTGCTTTCGTCCTCTTTTGCGCTTGGTGT GCACTGAACAGTGCAAAAGCGAAAAGGCAATTTGTCAATGAATGGGCAGCGGAGATCCCC GGGGGCCCGGAAGCAGCCTCGGCCATCGCCGAGGAGCTGGGCTATGACCTTTTGGGTCAG ATTGGTTCACTTGAAAATCACTACTTATTCAAACATAAAAACCACCCCAGAAGGTCTCGA AGGAGTGCCTTTCATATCACTAAGAGATTATCTGATGATGATCGTGTGATATGGGCTGAA CAACAGTATGAAAAAGAAAGAAGTAAACGTTCAGCTCTAAGGGACTCAGCACTAAATCTC TTCAATGATCCCATGTGGAATCAGCAATGGTACTTGCAAGATACCAGGATGACGGCAGCC CTGCCCAAGCTGGACCTTCATGTGATACCTGTTTGGCAAAAAGGCATTACGGGCAAAGGA GTTGTTATCACCGTACTGGATGATGGTTTGGAGTGGAATCACACGGACATTTATGCCAAC TATGATCCAGAGGCTAGCTATGATTTTAATGATAATGACCATGATCCATTTCCCCGATAT GATCCCACAAACGAGAACAAACACGGGACCAGATGTGCAGGAGAAATTGCCATGCAAGCA AATAATCACAAATGCGGGGTTGGAGTTGCATACAATTCCAAAGTTGGAGGCATAAGAATG CTGGATGGCATTGTGACGGATGCTATTGAGGCCAGTTCAATTGGATTCAATCCTGGACAC GTGGATATTTACAGTGCAAGCTGGGGCCCTAATGATGATGGGAAAACTGTGGAGGGGCCT GGCCGGCTAGCCCAGAAGGCTTTTGAATATGGTGTCAAACAGGGGAGACAGGGGAAGGGG TCCATCTTCGTCTGGGCTTCGGGAAACGGGGGGCGTCAGGGAGATAATTGTGACTGTGAT GGCTACACAGACAGCATCTACACCATCTCCATCAGCAGTGCCTCCCAGCAAGGCCTATCC CCCTGGTACGCTGAGAAGTGCTCCTCCACACTGGCCACCTCTTACAGCAGCGGAGATTAC ACCGACCAGAGAATCACGAGCGCTGACCTGCACAATGACTGCACGGAGACGCACACAGGC ACCTCGGCCTCTGCACCTCTGGCTGCTGGCATCTTCGCTCTGGCCCTGGAAGCAAACCCA AATCTCACCTGGCGAGATATGCAGCACCTGGTTGTCTGGACCTCTGAGTATGACCCGCTG GCCAATAACCCTGGATGGAAAAAGAATGGAGCAGGCTTGATGGTGAATAGTCGATTTGGA TTTGGCTTGCTAAATGCCAAAGCTCTGGTGGATTTAGCTGACCCCAGGACCTGGAGGAGC GTGCCTGAGAAGAAAGAGTGTGTTGTAAAGGACAATGACTTTGAGCCCAGAGCCCTGAAA GCTAATGGAGAAGTTATCATTGAAATTCCAACAAGAGCTTGTGAAGGACAAGAAAATGCT ATCAAGTCCCTGGAGCATGTACAATTTGAAGCAACAATTGAATATTCCCGAAGAGGAGAC CTTCATGTCACACTTACTTCTGCTGCTGGAACTAGCACTGTGCTCTTGGCTGAAAGAGAA CGGGATACATCTCCTAATGGCTTTAAGAATTGGGACTTCATGTCTGTTCACACATGGGGA GAGAACCCTATAGGTACTTGGACTTTGAGAATTACAGACATGTCTGGAAGAATTCAAAAT GAAGGAAGAATTGTGAACTGGAAGCTGATTTTGCACGGGACCTCTTCTCAGCCAGAGCAT ATGAAGCAGCCTCGTGTGTACACGTCCTACAACACTGTTCAGAATGACAGAAGAGGGGTG GAGAAGATGGTGGATCCAGGGGAGGAGCAGCCCACACAAGAGAACCCTAAGGAGAACACC CTGGTGTCCAAAAGCCCCAGCAGCAGCAGCGTAGGGGGCCGGAGGGATGAGTTGGAGGAG GGAGCCCCTTCCCAGGCCATGCTGCGACTCCTGCAAAGTGCTTTCAGTAAAAACTCACCG CCAAAGCAATCACCAAAGAAGTCCCCAAGTGCAAAGCTCAACATCCCTTATGAAAACTTC TACGAAGCCCTGGAAAAGCTGAACAAACCTTCCCAGCTTAAAGACTCTGAAGACAGTCTG TATAATGACTATGTTGATGTTTTTTATAACACTAAACCTTACAAGCACAGAGACGACCGG CTGCTTCAAGCTCTGGTGGACATTCTGAATGAGGAAAATTAA Clone variation with respect to NM_000439.4 >OriGene 5' read for NM_000439 unedited
GGGTTCGGATATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGAAGC GCTTCACTGAGCGCTCGCCGCCGCCCAGCCTCTCCTCTCGCGCCTCCTAGCTCTTCGCAG AGCAACCAGGAGCCAGGAGTGGTCCAGAGCCCGAGGGTGGGAAGGGGGAGTCTGTCTGGC TTTTCTCCTATCTTGCTTCTTTTTCCTCTTCCCTTCCCACTCTTGTTCAAGCGAGTGTGT GAGCTATGGAGCGAAGAGCCTGGAGTCTGCAGTGCACTGCTTTCGTCCTCTTTTGCGCTT GGTGTGCACTGAACAGTGCAAAAGCGAAAAGGCAATTTGTCAATGAATGGGCAGCGGAGA TCCCCGGGGGCCCGGAAGCAGCCTCGGCCATCGCCGAGGAGCTGGGCTATGACCTTTTGG GTCAGATTGGTTCACTTGAAAATCACTACTTATTCAAACATAAAAACCACCCCAGAAGGT CTCGAAGGAGTGCCTTTCATATCACTAAGAGATTATCTGATGATGATCGTGTGATATGGG CTGAACAACAGTATGAAAAAGAAAGAAGTAAACGTTCAGCTCTAAGGGACTCAGCACTAA ATCTCTTCAATGATCCCATGTGGAATCAGCAATGGTACTTGCAAGATACCAGGATGACGG CAGCCCTGCCCAAGCTGGACCTTCATGTGATACCTGTTTGGCAAAAAGGCATTACGGGCA AAGGAGTTGTTATCACCGTACTGGATGATGGTTTGGAGTGGAATCACACGGACATTTATG CCAACTATGATCCAGAGGCTAGCTATNGATTTAATGGATATGACCATGATCCATTTCCCC GATATGATCCCACAAACGAGAACANACACGGGACCAGATGTGCAGGNAGAAATGCCATGN NCAGCAATAATCACAATGCG >OriGene 3' read for NM_000439 unedited
NTTAGCTATGGCCCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTGGG AAAGTGGTATATATCATTTCTCCCAACATTTATTTTGTGGCTGAGAAAGGAGACAGGTAT AGAAAACTTTCAGAAGGAGAAACAGAGGAAAGACCAGGCATGCAGCACGAGTCATAACCA CCTCTGGATCCACGGACGCCTCTGGCACCACAGACACAGTGCACCAGGCAAAGAATGTTC GAAAATTGCTCAAACCAGAGCATTCAGATTTCAAGATCTGTTTCTAGATCTAAATAATCC AGCTTTTGACCAACATGACCAGATTTTGACTACAGGAATGAGTGGCACTTTGGAATAGAA AGATCTAGGGAATGAGTCTTACAGTGAACTCCTTCATTAGGCCTACCAAATACTTTCTTG GCATCAAAATTTGAAGAACCACATGGACAAGACAGGGAACAAGCTACAGAAACAGTTTCT GACTCCTTCTCATTCTTTGCAGATTATGTTTTCAAATTAATTGATATCCCAGGTTTTGCC TTTTCTTTTGAGAATTGAAATGGGCTCTAATGCAGTGTTCTAATGTAGTGTAAGAGCTTT TTGTCAACTGTGACTCCAGAAAGAACAAAACACTTCACTTGTGCAGACAGGAAAGATGTG TTTTGAAATACCTATGATCAATTCTGGAAGTTGAACTTCCTGCTTGAGCTCATCCCCTTC ACATGTACAGTTTAGGGAGAAAAAGAAAAGGTGCCACAAAGGATATTATAAGCATAAGAA TTCATGACANNAACACCACTTCAGACACAGGCAANATCGCAGGGTAAGGAAGAGCATGAA TATTTCCACTTGGGACCCACACTTATTTTAATTTNCTCATTCAGAATGTCACCAGAGCTT GAGCAGNNCGTCGTCTCTTGCTTGTAAGGTTTAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000439 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000439.3, NP_000430.3 |
| RefSeq Size | 5054 bp |
| RefSeq ORF | 2262 bp |
| Locus ID | 5122 |
| UniProt ID | P29120 |
| Domains | Peptidase_S8, P |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| Gene Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221711 | PCSK1 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1 |
CNY 8,272.00 |
|
| RC221711L1 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221711L2 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC221711L3 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1, Myc-DDK-tagged |
CNY 10,672.00 |
|
| RC221711L4 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1, mGFP tagged |
CNY 10,672.00 |
|
| RG221711 | PCSK1 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 1 |
CNY 9,872.00 |
