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Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) (NM_000414) Human Untagged Clone
CAT＃: SC119874
HSD17B4 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2
CNY 5,400.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DBP; MFE-2; MFP-2; MPF-2; PRLTS1; SDR8C1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119874 sequence for NM_000414 edited (data generated by NextGen Sequencing)
ATGGGCTCACCGCTGAGGTTCGACGGGCGGGTGGTACTGGTCACCGGCGCGGGGGCAGGA TTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAAGAGGAGCGTTAGTTGTTGTGAATGAT TTGGGAGGGGACTTCAAAGGAGTTGGTAAAGGCTCCTTAGCTGCTGATAAGGTTGTTGAA GAAATAAGAAGGAGAGGTGGAAAAGCAGTGGCCAACTATGATTCAGTGGAAGAAGGAGAG AAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAAGAATAGATGTTGTGGTCAACAATGCT GGAATTCTGAGGGATCGTTCCTTTGCTAGGATAAGTGATGAAGACTGGGATATAATCCAC AGAGTTCATTTGCGGGGTTCATTCCAAGTGACACGGGCAGCATGGGAACACATGAAGAAA CAGAAGTATGGAAGGATTATTATGACTTCATCAGCTTCAGGAATATATGGCAACTTTGGC CAGGCCAATTATAGTGCTGCAAAGTTGGGTCTTCTGGGCCTTGCAAATTCTCTTGCAATT GAAGGCAGGAAAAGCAACATTCATTGTAACACCATTGCTCCTAATGCGGGATCACGGATG ACTCAGACAGTTATGCCTGAAGATCTTGTGGAAGCCCTGAAGCCAGAGTATGTGGCACCT CTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGGCTTGTTTGAGGTTGGA GCAGGATGGATTGGAAAATTACGCTGGGAGCGGACTCTTGGAGCTATTGTAAGACAAAAG AATCACCCAATGACTCCTGAGGCAGTCAAGGCTAACTGGAAGAAGATCTGTGACTTTGAG AATGCCAGCAAGCCTCAGAGTATCCAAGAATCAACTGGCAGTATAATTGAAGTTCTGAGT AAAATAGATTCAGAAGGAGGAGTTTCAGCAAATCATACTAGTCGTGCAACGTCTACAGCA ACATCAGGATTTGCTGGAGCTATTGGCCAGAAACTCCCTCCATTTTCTTATGCTTATACG GAACTGGAAGCTATTATGTATGCCCTTGGAGTGGGAGCGTCAATCAAGGATCCAAAAGAT TTGAAATTTATTTATGAAGGAAGTTCTGATTTCTCCTGTTTGCCCACCTTCGGAGTTATC ATAGGTCAGAAATCTATGATGGGTGGAGGATTAGCAGAAATTCCTGGACTTTCAATCAAC TTTGCAAAGGTTCTTCATGGAGAGCAGTACTTAGAGTTATATAAACCACTTCCCAGAGCA GGAAAATTAAAATGTGAAGCAGTTGTTGCTGATGTCCTAGATAAAGGATCCGGTGTAGTG ATTATTATGGATGTCTATTCTTATTCTGAGAAGGAACTTATATGCCACAATCAGTTCTCT CTCTTTCTTGTTGGCTCTGGAGGCTTTGGTGGAAAACGGACATCAGACAAAGTCAAGGTA GCTGTAGCCATACCTAATAGACCTCCTGATGCTGTACTTACAGATACCACCTCTCTTAAT CAGGCTGCTTTGTACCGCCTCAGTGGAGACTGGAATCCCTTACACATTGATCCTAACTTT GCTAGTCTAGCAGGTTTTGACAAGCCCATATTACATGGATTATGTACATTTGGATTTTCT GCCAGGCGTGTGTTACAGCAGTTTGCAGATAATGATGTGTCAAGATTCAAGGCAATTAAG GCTCGTTTTGCAAAACCAGTATATCCAGGACAAACTCTACAAACTGAGATGTGGAAGGAA GGAAACAGAATTCATTTTCAAACCAAGGTCCAAGAAACTGGAGACATTGTCATTTCAAAT GCATATGTGGATCTTGCACCAACATCTGGTACTTCAGCTAAGACACCCTCTGAGGGCGGG AAGCTTCAGAGTACCTTTGTATTTGAGGAAATAGGACGCCGCCTAAAGGATATTGGGCCT GAGGTGGTGAAGAAAGTAAATGCTGTATTTGAGTGGCATATAACCAAAGGCGGAAATATT GGGGCTAAGTGGACTATTGACCTGAAAAGTGGTTCTGGAAAAGTGTACCAAGGCCCTGCA AAAGGTGCTGCTGATACAACAATCATACTTTCAGATGAAGATTTCATGGAGGTGGTCCTG GGCAAGCTTGACCCTCAGAAGGCATTCTTTAGTGGCAGGCTGAAGGCCAGAGGGAACATC ATGCTGAGCCAGAAACTTCAGATGATTCTTAAAGACTACGCCAAGCTCTGA Clone variation with respect to NM_000414.3 >OriGene 5' read for NM_000414 unedited
NGGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCTTGTTCG TGTGTGTGTCGTTGCAGGCCTTATTCATGGGCTCACCGCTGAGGTTCGACGGGCGGGTGG TACTGGTCACCGGCGCGGGGGCAGGATTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAA GAGGAGCGTTAGTTGTTGTGAATGATTTGGGAGGGGACTTCAAAGGAGTTGGTAAAGGCT CCTTAGCTGCTGATAAGGTTGTTGAAGAAATAAGAAGGAGAGGTGGAAAAGCAGTGGCCA ACTATGATTCAGTGGAAGAAGGAGAGAAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAA GAATAGATGTTGTGGTCAACAATGCTGGAATTCTGAGGGATCGTTCCTTTGCTAGGATAA GTGATGAAGACTGGGATATAATCCACAGAGTTCATTTGCGGGGTTCATTCCAAGTGACAC GGGCAGCATGGGAACACATGAAGAAACAGAAGTATGGAAGGATTATTATGACTTCATCAG CTTCAGGAATATATGGCAACTTTGGCCAGGCCAATTATAGTGCTGCAAAGTTGGGTCTTC TGGGCCTTGCAAATTCTCTTGCAATTGAAGGCAGGAAAAGCAACATTCATTGTAACACCA TTGCTCCTAATGCGGGATCACGGATGACTCAGACAGTTATGCCTGAAGATCTTGTGGAAG CCCTGAAGCCAGAGTATGTGGCACCTCTTGTCCTTTGGCTNTGTCACGAGAAGTGTGAGG AGAATGGTGCCTTGTTTGAGGTTGGAGCCAGATGGATTGGAAATTACGCTNGGAGCGACT CTTGNAGCTATTGTAGACAAAGATCACCCCATGACTCCTGAGCAGTCAAGGCTACTGGAG AA >OriGene 3' read for NM_000414 unedited
GAACGCGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAAGTATCAAAGG CCTTTATTGATTTAATTTTCCTTTCAACTTATCAATGCTATCACAAATTCTAAGGAAACA GGACTTGGGCAAAATTTTTTATTATGAAGATACAGATCTAAGAACAGAAGGATAATTATG TGCTAGCTTATATATAGTTCCTTGTAAAAATAATGATACATGAAAAATTAGTAGAAAATG AAAATCTGCAGTTATCTGATATCTGAAAATGTTAAGCAATTTCCCCTGCATCTTAGTTCT AATCACTTTTGCAGAATAATCAAGCATATTTGGGTGAAGAGAGTATTTAATGATTCCATT TTTATTAATAGTGTAGTGTGCCCTTCAGAGCTTGGCGTAGTCTTTAAGAATCATCTGAAG TTTCTGGCTCAGCATGATGTTCCCTCTGGCCTTCAGCCTGCCACTAAAGAATGCCTTCTG AGGGTCAAGCTTGCCCAGGACCACCTCCATGAAATCTTCATCTGAAAGTATGATTGTTGT ATCAGCAGCACCTTTTGCAGGGCCTTGGTACACTTTTCCAGAACCACTTTTCAAGTCAAT AGTCCACTTAGCCCCAATATTTCCGCCTTTGGTTATATGCCACTCAAATACAGCATTTAC TTTTTTCACCACCTCATGCCCAATATCCTTTAAGCGGCGCCCCTATTCCTCAAATACAAA AGTACTCTGAAACTTCCCCGCCTCAGAAGGTGTCTTAACTGGAGACCAGAAGTTGGGGCA AGATCCCCATATGCATTTTGAATGACAATGTTTCCCACTCTCTTGAACCTTGGCTGGAAA AAGAATTCTGTTTCCTTCCTTACACTTCTAATTTGAAAAAGCTGCCCTGGATATATGGTT TTGACAACCAGCCTTATTGGCACTGATCTGGACACCTATTTTCGGAACTGCTGTACACAC GCTGGTAAAATCACATGGCATAACCCGTCAATGGGCTTGACAAACGGGCACTAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000414 |
| Insert Size | 2590 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000414.1, NP_000405.1 |
| RefSeq Size | 2593 bp |
| RefSeq ORF | 2211 bp |
| Locus ID | 3295 |
| UniProt ID | P51659 |
| Domains | adh_short, MaoC_dehydratas, SCP2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Primary bile acid biosynthesis |
| Gene Summary | The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200460 | HSD17B4 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2 |
CNY 5,392.00 |
|
| RC200460L3 | Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2, Myc-DDK-tagged |
CNY 8,170.00 |
|
| RC200460L4 | Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2, mGFP tagged |
CNY 7,792.00 |
|
| RG200460 | HSD17B4 (tGFP-tagged) - Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2 |
CNY 6,992.00 |
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