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BARD1 (NM_000465) Human Untagged Clone
CAT＃: SC119847
BARD1 (untagged)-Human BRCA1 associated RING domain 1 (BARD1)
CNY 5,696.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000465 edited
GAATTCGGCACGAGGCTTGGCCGGTTTCGAGTCGCTGACCTGCAGCTTCCCTGTGGTTTC CCGAGGCCTCCTTGCTTCCCGCTCTCCGAGGAGCCTTTCATCCGAAGGCGGGACGATGCC GGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGATCCGCTCCGGGAACGAGCCTCGTTC CGCGCCCGCCATGGAACCGGATGGTCGCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGA CCGCCTGGAGAAGCTGCTGCGCTGCTCGCGTTGTACTAACATTCTGAGAGAGCCTGTGTG TTTAGGAGGATGTGAGCACATCTTCTGTAGTAATTGTGTAAGTGACTGCATTGGAACTGG ATGTCCAGTGTGTTACACCCCGGCCTGGATACAAGACTTGAAGATAAATAGACAACTGGA CAGCATGATTCAACTTTGTAGTAAGCTTCGAAATTTGCTACATGACAATGAGCTGTCAGA TTTGAAAGAAGATAAACCTAGGAAAAGTTTGTTTAATGATGCAGGAAACAAGAAGAATTC AATTAAAATGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCTTC AGTGCAAACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATGAATT TGTTTCCCCAAGTCCTCCTGCAGATGTTTCTGAGAGGGCTAAAAAGGCTTCTGCAAGATC TGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAATCAACCAAAAATGGAATTTAGAGGC AGAAAAAGAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTT CTGTAGCCAACCATCTGTTATCTCCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGC AAGTGGCTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTACCATT GGCTGAGCAAATAGAGTCTCCAGACACTAAGAGCAGGAATGAAGTAGTGACTCCTGAGAA GGTCTGCAAAAATTATCTTACATCTAAGAAATCTTTGCCATTAGAAAATAATGGAAAACG TGGCCATCACAATAGACTTTCCAGTCCCATTTCTAAGAGATGTAGAACCAGCATTCTGAG CACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATATACCATTGCCTGAATG TTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACATCAGGGAGCAAAAACAGTAA CATGTCCGATGAATTCATTAGTCTTTCACCAGGTACACCACCTTCTACATTAAGTAGTTC AAGTTACAGGCGAGTGATGTCTAGTCCCTCAGCAATGAAGCTGTTGCCCAATATGGCTGT GAAAAGAAATCATAGAGGAGAGACTTTGCTCCATATTGCTTCTATTAAGGGCGACATACC TTCTGTTGAATACCTTTTACAAAATGGAAGTGATCCAAATGTTAAAGACCATGCTGGATG GACACCATTGCATGAAGCTTGCAATCATGGGCACCTGAAGGTAGTGGAATTATTGCTCCA GCATAAGGCATTGGTGAACACCACCGGGTATCAAAATGACTCACCACTTCACGATGCAGC CAAGAATGGGCACGTGGATATAGTCAAGCTGTTACTTTCCTATGGAGCCTCCAGAAATGC TGTTAATATATTTGGTCTGCGGCCTGTCGATTATACAGATGATGAAAGTATGAAATCGCT ATTGCTGCTACCAGAGAAGAATGAATCATCCTCAGCTAGCCACTGCTCAGTAATGAACAC TGGGCAGCGTAGGGATGGACCTCTTGTACTTATAGGCAGTGGGCTGTCTTCAGAACAACA GAAAATGCTCAGTGAGCTTGCAGTAATTCTTAAGGCTAAAAAATATACTGAGTTTGACAG TACAGTAACTCATGTTGTTGTTCCTGGTGATGCAGTTCAAAGTACCTTGAAGTGTATGCT TGGGATTCTCAATGGATGCTGGATTCTAAAATTTGAATGGGTAAAAGCATGTCTACGAAG AAAAGTATGTGAACAGGAAGAAAAGTATGAAATTCCTGAAGGTCCACGCAGAAGCAGGCT CAACAGAGAACAGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTATTTGTGGGGAAC CTTCAAACACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCAGGTGGGGGCCAGAT CCTCAGTAGAAAGCCCAAGCCAGACAGTGACGTGACTCAGACCATCAATACAGTCGCATA CCATGCGAGACCCGATTCTGATCAGCGCTTCTGCACACAGTATATCATCTATGAAGATTT GTGTAATTATCACCCAGAGAGGGTTCGGCAGGGCAAAGTCTGGAAGGCTCCTTCGAGCTG GTTTATAGACTGTGTGATGTCCTTTGAGTTGCTTCCTCTTGACAGCTGAATATTATACCA GATGAACATTTCAAATTGAATTTGCACGGTTTGTGAGAGCCCAGTCATTGTACTGTTTTT AATGTTCACATTTTTACAAATAGGTAGAGTCATTCATATTTGTCTTTGAATCAAAAAAAA AAAAAAAAAACTCGAC >OriGene 5' read for NM_000465 unedited
GGAATTTGTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGCTTGGCCGGTTTC GAGTCGCTGACCTGCAGCTTCCCTGTGGTTTCCCGAGGCCTCCTTGCTTCCCGCTCTCCG AGGAGCCTTTCATCCGAAGGCGGGACGATGCCGGATAATCGGCAGCCCGAGAACCGGCAG CCGAGGATCCGCTCCGGGAACGAGCCTCGTTCCGCGCCCGCCATGGAACCGGATGGTCGC GGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTGCTGCGCTGCTCG CGTTGTACTAACATTCTGAGAGAGCCTGTGTGTTTAGGAGGATGTGAGCACATCTTCTGT AGTAATTGTGTAAGTGACTGCATTGGAACTGGATGTCCAGTGTGTTACACCCCGGCCTGG ATACAAGACTTGAAGATAAATAGACAACTGGACAGCATGATTCAACTTTGTAGTAAGCTT CGAAATTTGCTACATGACAATGAGCTGTCAGATTTGAAAGAAGATAAACCTAGGAAAAGT TTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTTAGCCCTCGAAGT AAGAAAGTCAGATATGTTGTGAGTAAAGCTTCAGTGCAAACCCAGCCTGCAATAAAAAAA GATGCAAGTGCTCAGCAAGACTCATATGAATTTGTTTCCCCAAGTCCTCCTGCAGATGTT TCTGAGAGGGCTAAAAAGGCTTCTGNCAGATCTGGNAAAAAGCANAAAAAGAAACTTTAG CTGAAATCAACAAAAATGGAATTTAGAGGCAGAAAAAGAGATGGTGAATTTGACTCCCAA GAGAAATCTAGCAAAGCTGGTATCCTTCTGTAGCCACCATCTGTTATCTCCAGTCCTCAG ATAAATGGTGAATAGACTACTAGCAGTGGCTCCTGACA >OriGene 3' read for NM_000465 unedited
CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGATTCAAANACAAATATGAAT GACTCTACCTATTTGTAAAAATGTGAACATTAAAAACAGTACAATGACTGGGCTCTCACA AACCGTGCAAATTCAATTTGAAATGTTCATCTGGTATAATATTCAGCTGTCAAGAGGAAG CAACTCAAAGGACATCACACAGTCTATAAACCAGCTCGAAGGAGCCTTCCAGACTTTGCC CTGCCGAACCCTCTCTGGGTGATAATTACACAAATCTTCATAGATGATATACTGTGTGCA GAAGCGCTGATCAGAATCGGGTCTCGCATGGTATGCGACTGTATTGATGGTCTGAGTCAC GTCACTGTCTGGCTTGGGCTTTCTACTGAGGATCTGGCCCCCACCTGCAGTGACGAGCTT AATAAGGTTGTCCTTTGGATGGTGTTTGAAGGTTCCCCACAAATAGAAGTAGCATCCATC AAACAGCTTTGGCAACAGCTGTTCTCTGTTGAGCCTGCTTCTGCGTGGACCTTCAGGAAT TTCATACTTTTCTTCCTGTTCACATACTTTTCTTCGTAGACATGCTTTTACCCATTCAAA TTTTAGAATCCAGCATCCATTGAGAATCCCAAGCATACACTTCAAGGTACTTTGAACTGC ATCACCAGGAACAACAACATGAGTTACTGTACTGTCAAACTCAGTATATTTTTTAGCCTT AAGAATTACTGNCAGCTCACTGAGCATTTTCTGTTGTTCTGAAGACAGCCCACTGCCTAT AAGTACAAGAGGTCCATCCCTACGCTGCCCAGTGTTCATTACTGAACAGTGGCTAGCTGA AGATGATTCATTCTTCTCCTGGTAGCAGCAATAGCCGATTCATACCTTTATCATCTGTAT AAACGACAGCCGCAAACCAATATATAACAGCATTCCTGGAGCTCCATAGAAAGAAAGCTT GCTTTTTCAGTGCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000465 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000465.1, NP_000456.1 |
| RefSeq Size | 2530 bp |
| RefSeq ORF | 2334 bp |
| Locus ID | 580 |
| UniProt ID | Q99728 |
| Domains | BRCT, ANK |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Transcriptional Autoregulation by BRCA1
,Adriana De Siervi, Paola De Luca, Jung S. Byun, Li Jun Di, Temesgen Fufa, Cynthia M. Haggerty, Elba Vazquez, Cristian Moiola, Dan L. Longo, and Kevin Gardner,
Cancer Res., Jan 2010; 70: 532 - 542
[BARD1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211511 | BARD1 (Myc-DDK-tagged)-Human BRCA1 associated RING domain 1 (BARD1) |
CNY 8,536.00 |
|
| RC211511L1 | Lenti ORF clone of Human BRCA1 associated RING domain 1 (BARD1), Myc-DDK-tagged |
CNY 10,936.00 |
|
| RC211511L2 | Lenti ORF clone of Human BRCA1 associated RING domain 1 (BARD1), mGFP tagged |
CNY 5,890.00 |
|
| RC211511L3 | Lenti ORF clone of Human BRCA1 associated RING domain 1 (BARD1), Myc-DDK-tagged |
CNY 10,936.00 |
|
| RC211511L4 | Lenti ORF clone of Human BRCA1 associated RING domain 1 (BARD1), mGFP tagged |
CNY 10,936.00 |
|
| RG211511 | BARD1 (tGFP-tagged) - Human BRCA1 associated RING domain 1 (BARD1) |
CNY 10,136.00 |
