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NCF4 (NM_000631) Human Untagged Clone
CAT＃: SC119769
NCF4 (untagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1
CNY 3,656.00
CNY 5,510.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGD3; NCF; P40PHOX; SH3PXD4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119769 sequence for NM_000631 edited (data generated by NextGen Sequencing)
ATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGATGATGTT GCCATCTCGGCCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTT TTCGTCATCGAGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGC CAGTTCCATGCTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAGAGCAGT GCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAACAGGAG ATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGCCTGCCG GTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCA GAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTG TCCCCACAGGGCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGAC TTCACTGGAAACAGCAAACTGGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTC AGTCGGATCAACAAAGACTGGCTGGAGGGCACTGTCCGGGGAGCCACGGGCATCTTCCCT CTCTCCTTCGTGAAGATCCTCAAAGACTTCCCTGAGGAGGACGACCCCACCAACTGGCTG CGTTGCTACTACTACGAAGACACCATCAGCACCATCAAGGACATCGCGGTGGAGGAAGAT CTCAGCAGCACTCCCCTATTGAAAGACCTGCTGGAGCTCACAAGGCGGGAGTTCCAGAGA GAGGACATAGCTCTGAATTACCGGGACGCTGAGGGGGATCTGGTTCGGCTGCTGTCGGAT GAGGACGTAGCGCTCATGGTGCGGCAGGCTCGTGGCCTCCCCTCCCAGAAGCGCCTCTTC CCCTGGAAGCTGCACATCACGCAGAAGGACAACTACAGGGTCTACAACACGATGCCATGA Clone variation with respect to NM_000631.4 >OriGene 5' read for NM_000631 unedited
AGGAATTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGCCCAAGGCAG CTCCTGGGGACTCCCAGACCACAGGCTGAGACGAGACGCAGGGTGGCTGGAGGAAGTGAG AGGTGAACTCAGCCTGGGACTGGCTGGGCGAGACTCTCCACCTGCTCCCTGGGACCATCG CCCACCATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGAT GATGTTGCCATCTCGGCCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCAC TTTGTTTTCGTCATCGAGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGC TACCGCCAGTTCCATGCTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAG AGCAGTGCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAA CAGGAGATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGC CTGCCGGTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTAT GACTCAGAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAG AGCGTGTCCCCACAGGGCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTA TTTGACTTCACTGGAAACAGCAAACTGGAGCTGAATTTAAAGCTGGAGATGTGATCTTCC TNCTCAGTCGNATCAACNAGACTGGCTGGAGGGCACTGTCCGGNNGAGCCACGGCATCTT CCCTCTCTTCTTTCGTGAAGAACTCAAAGACTTCCCTGAGAGGACGACCCCNACACTGGC TGCGNTGCTACTACTACGAAGACACATCAGCACATCAGGACATCGCGGTGGAGGAAGACT CACAGACTNCCCTATGAANGACTGCG >OriGene 3' read for NM_000631 unedited
TTTTTTTTTTTTTTTTTCCCCTTTCAAAGGAATGGTTTATTTAAATCAGATGTAAATAGT AGAAAGAGACAGATTACAAGCCCCACGGGTCCATTAGCCTCAAAAAAGGAAGTCTGCCCA TCCTCCCAGGTTTTCCTGGTCCCAATCTCCTCTGAGAGCTGAAGGTTTTTGCTGGTGTCC CCTCACTGCTCCAGGGACACCGTCAGCTCATGGCATCGTGTTGTAGACCCTGTAGTTGTC CTTCTGCGTGATGTGCAGCTTCCAGGGGAAGAGGCGCTTCTGGGAGGGGAGGCCACGAGC CTGCCGCACCATGAGCGCTACGTCCTCATCCGACAGCAGCCGAACCAGATCCCCCTCAGC GTCCCGGTAATTCAGAGCTATGTCCTCTCTCTGGAACTCCCGCCTTGTGAGCTCCAGCAG GTCTTTCAATAGGGGAGTGCTGCTGAGATCTTCCTCCACCGCGATGTCCTTGATGGTGCT GATGGTGTCTTCGTAGTAGTAGCAACGCAGCCAGTTGGTGGGGTCGTCCTCCTCAGGGAA GTCTTTGAGGATCTTCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000631 |
| Insert Size | 1460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000631.3, NP_000622.2 |
| RefSeq Size | 1386 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 4689 |
| UniProt ID | Q15080 |
| Domains | PB1, SH3, PX |
| Protein Pathways | Leukocyte transendothelial migration |
| Gene Summary | The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216094 | NCF4 (Myc-DDK-tagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1 |
CNY 3,656.00 |
|
| RC216094L1 | Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC216094L2 | Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216094L3 | Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216094L4 | Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG216094 | NCF4 (tGFP-tagged) - Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1 |
CNY 5,256.00 |
