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GUCY1A1 (NM_000856) Human Untagged Clone
CAT＃: SC119658
GUCY1A3 (untagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1
CNY 5,064.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3; GUCA3; GUCSA3; GUCY1A3; MYMY6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119658 sequence for NM_000856 edited (data generated by NextGen Sequencing)
ATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTTCTCCTTACTG GCACCAGGTCAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTCAGAGAGCTGC AAAGCAACCGTGCCCATCTGTCAAGACATTCCTGAGAAGAACATACAAGAAAGTCTTCCT CAAAGAAAAACCAGTCGGAGCCGAGTCTATCTTCACACTTTGGCAGAGAGTATTTGCAAA CTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCACTTCAGAGAACATTGGCAAAGCAC AAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAAAAAACAATTGCAGAG CAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGGTGAAGAGGTT TTTAAAATATGTTACGAGGAAGATGAAAACATCCTTGGGGTGGTTGGAGGCACCCTTAAA GATTTTTTAAACAGCTTCAGTACCCTTCTGAAACAGAGCAGCCATTGCCAAGAAGCAGGA AAAAGGGGCAGGCTTGAGGACGCCTCCATTCTATGCCTGGATAAGGAGGATGATTTTCTA CATGTTTACTACTTCTTCCCTAAGAGAACCACCTCCCTGATTCTTCCCGGCATCATAAAG GCAGCTGCTCACGTATTATATGAAACGGAAGTGGAAGTGTCGTTAATGCCTCCCTGCTTC CATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTGTTGTACTCCGTTCACATGAAA AGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTGGTGATTCCCACATCG CTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGATATGACAATTCTGCAA TTTGGCAATGGCATCAGAAGGCTGATGAACAGGAGAGACTTTCAAGGAAAGCCTAATTTT GAAGAATACTTTGAAATTCTGACTCCAAAAATCAACCAGACGTTTAGCGGGATCATGACT ATGTTGAATATGCAGTTTGTTGTACGAGTGAGGAGATGGGACAACTCTGTGAAGAAATCT TCAAGGGTTATGGACCTCAAAGGCCAAATGATCTACATTGTTGAATCCAGTGCAATCTTG TTTTTGGGGTCACCCTGTGTGGACAGATTAGAAGATTTTACAGGACGAGGGCTCTACCTC TCAGACATCCCAATTCACAATGCACTGAGGGATGTGGTCTTAATAGGGGAACAAGCCCGA GCTCAAGATGGCCTGAAGAAGAGGCTGGGGAAGCTGAAGGCTACCCTTGAGCAAGCCCAC CAAGCCCTGGAGGAGGAGAAGAAAAAGACAGTAGACCTTCTGTGCTCCATATTTCCCTGT GAGGTTGCTCAGCAGCTGTGGCAAGGGCAAGTTGTGCAAGCCAAGAAGTTCAGTAATGTC ACCATGCTCTTCTCAGACATCGTTGGGTTCACTGCCATCTGCTCCCAGTGCTCACCGCTG CAGGTCATCACCATGCTCAATGCACTGTACACTCGCTTCGACCAGCAGTGTGGAGAGCTG GATGTCTACAAGGTGGAGACCATTGGCGATGCCTATTGTGTAGCTGGGGGATTACACAAA GAGAGTGATACTCATGCTGTTCAGATAGCGCTGATGGCCCTGAAGATGATGGAGCTCTCT GATGAAGTTATGTCTCCCCATGGAGAACCTATCAAGATGCGAATTGGACTGCACTCTGGA TCAGTTTTTGCTGGCGTCGTTGGAGTTAAAATGCCCCGTTACTGTCTTTTTGGAAACAAT GTCACTCTGGCTAACAAATTTGAGTCCTGCAGTGTACCACGAAAAATCAATGTCAGCCCA ACAACTTACAGATTACTCAAAGACTGTCCTGGTTTCGTGTTTACCCCTCGATCAAGGGAG GAACTTCCACCAAACTTCCCTAGTGAAATCCCCGGAATCTGCCATTTTCTGGATGCTTAC CAACAAGGAACAAACTCAAAACCATGCTTCCAAAAGAAAGATGTGGAAGATGGCAATGCC AATTTTTTAGGCAAAGCATCAGGAATAGATTAG Clone variation with respect to NM_000856.4 1074 a=>g >OriGene 5' read for NM_000856 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGGGAGACACAGA CACAGAGAGACAAAGGCAAGGAGGACTGTCTGGGAGCCACGCGGGCGATACAGTTTCCGA GGCACGCCGCGTCCCGCCTAGCCTGTTGAACAGGTAGACATGAGCGACCCAAGCTGCGGA TTTGCGAGGCGCGCCCTGGAGCTGCTAGAGATCCGGAAGCACAGCCCCGAGGTGTGCGAA GCCACCAATCTCATATAAGAACTACAGCTCATCAGGAGGAGATCGCAGCAGGGTAAGAGA CACCAACACCATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTT CTCCTTACTGGCACCAGGTCAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTC AGAGAGCTGCAAAGCAACCGTGCCCATCTGTCAAGACATTCCTGAGAAGAACATACAAGA AAGTCTTCCTCAAAGAAAAACCAGTCGGAGCCGAGTCTATCTTCACACTTTGGCAGAGAG TATTTGCAAACTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCACTTCAGAGAACATT GGCAAAGCACAAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAAAAAAC AATTGCAGAGCAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGG TGAAAGAGTTTTTAAAATATGTTACGAGGAAGATGAAAACATCTTGGGGGTGTNTGCAGG CACCCTTAAAGATTTTNTAAACAGCTTCAGTACCCTTCTGAACAGAGCAGCCATTGCCAA GAAGCAGAAAAGGGGCAGCTTGAGGACGCCTCATTCTATGCTGATAGGNGG >OriGene 3' read for NM_000856 unedited
TTTAAAAAAANNNTTTCTTATGNACCGCGGNCCCGCATTTTANGATCGAGTTTTTTTTTT TTTTTTTGAAAAGCTGAAGTACTGAAGTGAGTACATTTTGTCATGTTAAACAGGAGAAAG ATTGTGACTTGGCTCCTGAAATTTTAATACTGCTTGTTAGCCATCTACAATCCCTAAAGT GCTTTCAGAGGCTCTACACATCTTCAATGAGTCAAACCCCAAAGACTTATAAATAGGTAT ATAGGTTGCTAATCTATTCCTGATGCTTTGCCTAAAAAATTGGCATTGCCATCTTCCACA TCTTTCTTTTGGAAGCATGGTTTTGAGTTTGTTCCTTGTTGGTAAGCATCCAGAAAATGG CAGATTCCGGGGATTTCACTANGGAAGTTTGGTGGAAGTTCCTCCCTTGATCGAGGNGGT AACACGANACCAGGACAGTCTTTGAGTAATCTGTAAAGTGTTGGGCTGACATTGATTTTT CGTGGTACACTGCANGACTCAAATTTGTAGCCAGAGTGACATTGNNTCCAAAAGACAGTA ACGGNGCATTTACTNCAACGACGCCAGCAAAACTGATCCAGAGTGCAGTCCAATTCGCAT CTTGATAGTNCTCCATGGNGAGACATAACTTCATAGAAAGCTCCATCATCTTTAGGGCCA TCANCGCTATCTGAAAACATGAGTTCACTCTCTTGGGTAATTCCCCAGCTTCCCATAGGC ATTGCCAATGGTCTCCACCTTGTGAAATCCAGCTTTCCACTGNTGGTCAAACGAGGGCCC AGGCCTTGACCTGGGGATGATTCAACGGGTAACCCTTGGACCAATGCCTTGGACCCACAA TTCTGAAAAAACTGGGGACCTTCTGAATTTTGGGTTGCCACATGCCTTTGCCAATTGTTG CCCCCCTCCGGAAAATTGGGCCCAAAGGTCCTGTGTTTTTTTTCCCTCCAAGCTGGGGGG TTTGCAGGGGCCCTAATTCCCACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000856 |
| Insert Size | 2570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000856.2, NP_000847.2 |
| RefSeq Size | 3015 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 2982 |
| UniProt ID | Q02108 |
| Domains | CYCc |
| Protein Families | Druggable Genome |
| Protein Pathways | Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction |
| Gene Summary | Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A). Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206063 | GUCY1A3 (Myc-DDK-tagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1 |
CNY 5,056.00 |
|
| RC206063L1 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, Myc-DDK-tagged |
CNY 7,456.00 |
|
| RC206063L2 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206063L3 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206063L4 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG206063 | GUCY1A3 (tGFP-tagged) - Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1 |
CNY 6,656.00 |
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