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NPY1R (NM_000909) Human Untagged Clone
CAT＃: SC119604
NPY1R (untagged)-Human neuropeptide Y receptor Y1 (NPY1R)
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NPY1-R; NPYR |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119604 sequence for NM_000909 edited (data generated by NextGen Sequencing)
ATGAATTCAACATTATTTTCCCAGGTTGAAAATCATTCAGTCCACTCTAATTTCTCAGAG AAGAATGCCCAGCTTCTGGCTTTTGAAAATGATGATTGTCATCTGCCCTTGGCCATGATA TTTACCTTAGCTCTTGCTTATGGAGCTGTGATCATTCTTGGTGTCTCTGGAAACCTGGCC TTGATCATAATCATCTTGAAACAAAAGGAGATGAGAAATGTTACCAACATCCTGATTGTG AACCTTTCCTTCTCAGACTTGCTTGTTGCCATCATGTGTCTCCCCTTTACATTTGTCTAC ACATTAATGGACCACTGGGTCTTTGGTGAGGCGATGTGTAAGTTGAATCCTTTTGTGCAA TGTGTTTCAATCACTGTGTCCATTTTCTCTCTGGTTCTCATTGCTGTGGAACGACATCAG CTGATAATCAACCCTCGAGGGTGGAGACCAAATAATAGACATGCTTATGTAGGTATTGCT GTGATTTGGGTCCTTGCTGTGGCTTCTTCTTTGCCTTTCCTGATCTACCAAGTAATGACT GATGAGCCGTTCCAAAATGTAACACTTGATGCGTACAAAGACAAATACGTGTGCTTTGAT CAATTTCCATCGGACTCTCATAGGTTGTCTTATACCACTCTCCTCTTGGTGCTGCAGTAT TTTGGTCCACTTTGTTTTATATTTATTTGCTACTTCAAGATATATATACGCCTAAAAAGG AGAAACAACATGATGGACAAGATGAGAGACAATAAGTACAGGTCCAGTGAAACCAAAAGA ATCAATATCATGCTGCTCTCCATTGTGGTAGCATTTGCAGTCTGCTGGCTCCCTCTTACC ATCTTTAACACTGTGTTTGATTGGAATCATCAGATCATTGCTACCTGCAACCACAATCTG TTATTCCTGCTCTGCCACCTCACAGCAATGATATCCACTTGTGTCAACCCCATATTTTAT GGGTTCCTGAACAAAAACTTCCAGAGAGACTTGCAGTTCTTCTTCAACTTTTGTGATTTC CGGTCTCGGGATGATGATTATGAAACAATAGCCATGTCCACGATGCACACAGATGTTTCC AAAACTTCTTTGAAGCAAGCAAGCCCAGTCGCATTTAAAAAAATCAACAACAATGATGAT AATGAAAAAATCTGA Clone variation with respect to NM_000909.5 >OriGene 5' read for NM_000909 unedited
CCCAATTACNATTTGTATACGACTTTTATAGGCGGCCGCGAATCGCACGAGGGAGGTCTG TGTCGAATAATGTGTGGCTTCTGTTGGATTGCTTTTCTTTCCAAAATTCCTAGGCAATGC TTCCCCGAGGTGTGCACCTTTGTGAGGTGTTTGTGGGGTTGGGGGAGCTTCAGGCGCTAC TCGCGGGACGCCGTCACGTGATCCGGGATGAGGTGGAGTTCGGCTTTAAGGAGGCGTCTC TTCCTAGCTTCATCAATCTTTAGGATCTGAGCAGGAGAAATACCAGCGGATCTTCCCCAC TCTGCTCCCTTCCATTCCCACCCTTCCTTCTTTAATAAGCAGGAGCGAAAAAGACAAATT CCAAAGAGGATTGTTCAGTTCAAGGGAATGAAGAATTCAGAATAATTTTGGTAAATGGAT TCCAATATGGGGAATAAGAATAAGCTGAACAGTTGACCTGCTTTGAAGAAACATACTGTC CATTTGTCTAAAATAATCTATAACAACCAAACCAATCAAAATGAATTCAACATTATTTTC CCAGGTTGAAAATCATTCAGTCCACTCTAATTTCTCAGAGAAGAATGCCCAGCTTCTGGC TTTTGAAAATGATGATTGTCATCTGCCCTTGGCCATGATATTTACCTTAGCTCTTGCTTA TGGAGCTGTGATCATTCTTGGTGTCTCTGGCAACCTGGCCTTGATCATAATCATCTTGAA ACTAAAGAGATGAGAAATGTTACCAACATCCTGATTGTGAACCTTTCCTTCTCAGACTTG CTTGGTGCCATCATGTGTCTCCCCTATACATTTGTCTACCATTAATGGACCACTGGGTCT TTGGTGAGGCGATGTGTAAGTTGAAACCCTTTGTGCATGTGTTTCAACACTGGGGCCATT TCTCTCTGGTCTCATGCTGCC >OriGene 3' read for NM_000909 unedited
ACAATATATGCATCTTTAATTAAAATGTNCACGCCCTCTTTAAAATCAAACACATGTAAT ACTTTATTCTGTATAATACATTGAAGTATGTCTTGTTCTTTAAAATATAGTTTCTCCAAA TGATTCTGAGGCTCAATCATGATTACAAGATACATGAAATTAGGCACATTAACATGACAC AACTATTCAGTAATCTTTACAGTGCATTATTTATAAGACGCCACAAGTCACACAGTTTAT ACAAGTTAAAATGGGCTATATGAAGTATCTGCAGTTTTTGTGTATTTTTCCCTGCTAGGT AAAGTGAATATTCCTTTAACATTGTACCTTGGTGTTACTTCCTTTGAAAGATGGACATTG AAAGTTAAAAATCAGTCACTATATAGGCACTGCTGTATGGAAAACGCATTTTGTGTTTCT ACAAATTGTAAGCGGAAATGCCTTTTGAGTACATATTTATGCCAGAAAGCTTGACAAACA CTGAACAGTCTGTAAAAAAAATGGAAAATCTCTTGACCATCAATTCAAATAAATATAATT TAACTATTTGCAGCAATACTAATTTACTTTATTAAATTAATTACTCTAAAAAGCTCTTGT TGTCAAGTACCAAAATGGTTTTGAAGTTAAGGAGCTGCTTTCTCTCTTCATTCCTGTGGG TGCCCCAATTCTCCATAATATTTGCAGCTGAGAAGTATGTCTTTTTATTCATTCTTAAAT TTTCATCTAAGTGACCATCAGTTGGGAGCAAATTAAATCCTCCCTCAGGTGAAAAAAATA AAAAACAAACAATAACCACCAAAAAACAGAAACCTCAAACCACTTCTGAATGACTTCAGA CTATCTTTCGGCTCCTGAATGCTGCCTTTGCATACTGTTACTATTGGAACACACTAAAAT GATGCCCTATCATAAGTAAGGATGGCCCAATCGTTGACATTCAATCAATCTAATCAATCA GATGACCCATCTATGACCGTTGGATGGAAGTCCTCTGAGTAGCTAATGAATACTTTAGAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000909 |
| Insert Size | 3370 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000909.4, NP_000900.1 |
| RefSeq Size | 2775 bp |
| RefSeq ORF | 1155 bp |
| Locus ID | 4886 |
| UniProt ID | P25929 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Gene Summary | This gene belongs to the G-protein-coupled receptor superfamily. The encoded transmembrane protein mediates the function of neuropeptide Y (NPY), a neurotransmitter, and peptide YY (PYY), a gastrointestinal hormone. The encoded receptor undergoes fast agonist-induced internalization through clathrin-coated pits and is subsequently recycled back to the cell membrane. Activation of Y1 receptors may result in mobilization of intracellular calcium and inhibition of adenylate cyclase activity. [provided by RefSeq, Aug 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mechanisms by which the orexigen NPY regulates anorexigenic a-MSH and TRH
,Nicole E. Cyr, Anika M. Toorie, Jennifer S. Steger, Matthew M. Sochat, Samantha Hyner, Mario Perello, Ronald Stuart, and Eduardo A. Nillni,
Am J Physiol Endocrinol Metab, Mar 2013; 304: E640 - E650.
[NPY1R]
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Other Versions
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| RC206057 | NPY1R (Myc-DDK-tagged)-Human neuropeptide Y receptor Y1 (NPY1R) |
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| RC206057L1 | Lenti ORF clone of Human neuropeptide Y receptor Y1 (NPY1R), Myc-DDK-tagged |
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| RC206057L2 | Lenti ORF clone of Human neuropeptide Y receptor Y1 (NPY1R), mGFP tagged |
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| RC206057L3 | Lenti ORF clone of Human neuropeptide Y receptor Y1 (NPY1R), Myc-DDK-tagged |
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| RC206057L4 | Lenti ORF clone of Human neuropeptide Y receptor Y1 (NPY1R), mGFP tagged |
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| RG206057 | NPY1R (tGFP-tagged) - Human neuropeptide Y receptor Y1 (NPY1R) |
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