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SRD5A1 (NM_001047) Human Untagged Clone
CAT＃: SC119479
SRD5A1 (untagged)-Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | S5AR 1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001047, the custom clone sequence may differ by one or more nucleotides
ATGGCAACGGCGACGGGGGTGGCGGAGGAGCGCCTGCTGGCCGCGCTCGCCTACCTGCAGTGCGCCGTGG GCTGCGCGGTCTTCGCGCGCAATCGTCAGACGAACTCAGTGTACGGCCGCCACGCGCTGCCCAGCCACAG GCTCCGAGTGCCGGCGCGGGCCGCCTGGGTGGTGCAGGAGCTGCCCTCGCTGGCCCTGCCGCTCTACCAG TACGCCAGCGAGTCCGCCCCGCGTCTCCGCAGCGCGCCCAACTGCATCCTCCTGGCCATGTTCCTCGTCC ACTACGGGCATCGGTGCTTAATTTACCCATTTCTGATGCGAGGAGGAAAGCCTATGCCACTGTTGGCGTG TACAATGGCGATTATGTTCTGTACCTGTAACGGCTATTTGCAAAGCAGATACTTGAGCCATTGTGCAGTG TATGCTGATGACTGGGTAACAGATCCCCGTTTTCTAATAGGTTTTGGCTTGTGGTTAACGGGCATGTTGA TAAACATCCATTCAGATCATATCCTAAGGAATCTCAGAAAACCAGGAGATACTGGATACAAAATACCAAG GGGAGGCTTATTTGAATACGTAACTGCAGCCAACTATTTTGGAGAAATCATGGAGTGGTGTGGCTATGCC CTGGCCAGCTGGTCTGTCCAAGGCGCGGCTTTTGCTTTCTTCACGTTTTGTTTTTTATCTGGTAGAGCAA AAGAGCATCATGAGTGGTACCTCCGGAAATTTGAAGAGTATCCAAAGTTCAGAAAAATTATAATTCCATT TTTGTTTTAA >OriGene 5' read for NM_001047 unedited
GGGCACCATTTGTATACGACTCACTATAGGCGGCCCGCGATTCGGCACGAGGCTTGAGAA CCCTTTCTGCAGATTCCCGGCAGTGCGGGACTCCGGTAGCCGCCCCTCCGGTAGCCGCCC CTCCTGCCCCCGCGCCGACGACCTAATGTTGCCCGCCGCGGCCTCTGGGGCATGGAGCAC GCTGCCCAGCCCTGGCGATGGCAACGGCCACGGGGGCTGGCGGCAGCAGCGCCTGCTGGC CGCGCTCGCCTACCTGCAGTGCGCCGCGGGCTGCGCGGTCTTCGCGCGGAATCGTCATAC GAACTCAGAGTACGGACGACACGCGCTGTCCAGACACAGGCTCCGAGCGCCGGCGCGGTC CGCCTGGGTGGTGCAGGAGCTGCCCTCCCTGGCCCTGCCGCTCTACCAATACGCCAGCGA GATCGTCCCGAGTATCCGCAGCGCGCCCAACTGCATCCTACTGGCCATGTTCCTCGTCCA CTACAGGCATAGGCGCTTAATTTACACATTTCTGATGCGAGGAGGAAAGCCTATGCCACT GCTGGCGTGTACAACGGCGAATATGCTCTGTACCTGTAACGGCTATTTCCCAAGCAGATA CTTGAGCCATTGTGCAGCGCATGCTGACGACTGGGTAACAGATCCCCCGCTTCTAATAAG TCTTGGCTTGTGGTTAACGGGCATGTTGATACACATCCATTCAGATCATATCCTAAGGAA TCTCAGAAAACCAGGAGATACTGGATACACAATACCAAGGGGNAGGCTTTATTTGATACG TCACTGCAGCCCACTATTTTGGGAGAATCATGGAGTGGGTGTGGGTATGCCCCTGCCCAG CTGGTCTGCCCAAGGCGCGGGTTTTGCTTTCTTTACGAATGGTTTTTATCTGGTCAAGCA AAAGACATCATGAGTGGGACCTT >OriGene 3' read for NM_001047 unedited
CGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAT GGCAAAGTCTATTTATTATGTTGTTAAAGACAACTGAAACAAATGGCATTTAAAAACTCC AGATGAAATGATAAGGCAAAGCAATGCCAGATGAATTCATCAAGATAAAAAGCAGTATGG CAATGTTTTTATCAAAGTAAAATTCAAGTCATGAAGCATTCAACAGGAAGATTATTTTAT ATTGATAACAGGTACAGGCTATGAGGGGAAAGCTTTGCAAAGCAACAACAGCAAAACAGA AAGCAAAAACTATTAAAAGGAAGATTACCTATGGAAAAAAAAAAAGGCTGTAAAAAATTT TAACAATTATTATGTTTCAATCAACAAAGAACAAACAGGATAAATCTTAAAAAGTGGTCT TGTTATTGTTACAAAAAGTGATCATTCTTTACACTACAAGGGAAAACTCAAAATCTACAA AAGAGAAATTTCACAGACAACAATCTCTACTATATCCATACGTGGGGAACAGAACAAAAA AACTAGGTCTAGACTAGAAGACAGAGAGAAACCATGTCAGACCAGAGCACATTGATGGCT CACGGTGAGTGACCCATGGCCTAAAGGTGCCTGTTTAGGGCCCAGGAGCCACTCACACAG GCAGAGCAGCTTACTCCGTATGAACCACCACCAGCACCCCTCCCTGCCCGGTGATGTCAC CCGGTGGGCTGGGCTGGAACAGTCCAGATGCCTTTGCCTCACCTGGAAAGGCCACGAGGA GCTTCCTCCTCTTCCCCAAGTTTCCACTTACCACAGCACCTGACACAATGTGGNCAAGTT TCCCCCGGGCATGACTGCACATGGCTACCCCAACCTTATGGCTCGAGAGAGCCTCTAAAC GTATTCACCCTTAGGGGNAGGAACAACCTGCATTGACCTGAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001047 |
| Insert Size | 2380 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001047.1, NP_001038.1 |
| RefSeq Size | 2285 bp |
| RefSeq ORF | 780 bp |
| Locus ID | 6715 |
| UniProt ID | P18405 |
| Domains | Steroid_dh |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Androgen and estrogen metabolism |
| Gene Summary | Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202747 | SRD5A1 (Myc-DDK-tagged)-Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1) |
CNY 3,600.00 |
|
| RC202747L1 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202747L2 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1), mGFP tagged |
CNY 5,890.00 |
|
| RC202747L3 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202747L4 | Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1), mGFP tagged |
CNY 6,000.00 |
|
| RG202747 | SRD5A1 (tGFP-tagged) - Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (SRD5A1) |
CNY 5,200.00 |
