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ADAM10 (NM_001110) Human Untagged Clone
CAT＃: SC119437
ADAM10 (untagged)-Human ADAM metallopeptidase domain 10 (ADAM10)
CNY 8,232.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AD10; AD18; CD156c; CDw156; HsT18717; kuz; MADM; RAK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_001110 edited
GCGAATTCGGCACGAGGCCGTTTCTCCTGCCAGGGGAGGTCCCGGCTTCCCGTGGAGGCT CCGGACCAAGCCCCTTCAGCTTCTCCCTCCGGATCGATGTGCTGCTGTTAACCCGTGAGG AGGCGGCGGCGGCGGCAGCGGCAGCGGAAGATGGTGTTGCTGAGAGTGTTAATTCTGCTC CTCTCCTGGGCGGCGGGGATGGGAGGTCAGTATGGGAATCCTTTAAATAAATATATCAGA CATTATGAAGGATTATCTTACAATGTGGATTCATTACACCAAAAACACCAGCGTGCCAAA AGAGCAGTCTCACATGAAGACCAATTTTTACGTCTAGATTTCCATGCCCATGGAAGACAT TTCAACCTACGAATGAAGAGGGACACTTCCCTTTTCAGTGATGAATTTAAAGTAGAAACA TCAAATAAAGTACTTGATTATGATACCTCTCATATTTACACTGGACATATTTATGGTGAA GAAGGAAGTTTTAGCCATGGGTCTGTTATTGATGGAAGATTTGAAGGATTCATCCAGACT CGTGGTGGCACATTTTATGTTGAGCCAGCAGAGAGATATATTAAAGACCGAACTCTGCCA TTTCACTCTGTCATTTATCATGAAGATGATATTAACTATCCCCATAAATACGGTCCTCAG GGGGGCTGTGCAGATCATTCAGTATTTGAAAGAATGAGGAAATACCAGATGACTGGTGTA GAGGAAGTAACACAGATACCTCAAGAAGAACATGCTGCTAATGGTCCAGAACTTCTGAGG AAAAAACGTACAACTTCAGCTGAAAAAAATACTTGTCAGCTTTATATTCAGACTGATCAT TTGTTCTTTAAATATTACGGAACACGAGAAGCTGTGATTGCCCAGATATCCAGTCATGTT AAAGCGATTGATACAATTTACCAGACCACAGACTTCTCCGGAATCCGTAACATCAGTTTC ATGGTGAAACGCATAAGAATCAATACAACTGCTGATGAGAAGGACCCTACAAATCCTTTC CGTTTCCCAAATATTGGTGTGGAGAAGTTTCTGGAATTGAATTCTGAGCAGAATCATGAT GACTACTGTTTGGCCTATGTCTTCACAGACCGAGATTTTGATGATGGCGTACTTGGTCTG GCTTGGGTTGGAGCACCTTCAGGAAGCTCTGGAGGAATATGTGAAAAAAGTAAACTCTAT TCAGATGGTAAGAAGAAGTCCTTAAACACTGGAATTATTACTGTTCAGAACTATGGGTCT CATGTACCTCCCAAAGTCTCTCACATTACTTTTGCTCACGAAGTTGGACATAACTTTGGA TCCCCACATGATTCTGGAACAGAGTGCACACCAGGAGAATCTAAGAATTTGGGTCAAAAA GAAAATGGCAATTACATCATGTATGCAAGAGCAACATCTGGGGACAAACTTAACAACAAT AAATTCTCACTCTGTAGTATTAGAAATATAAGCCAAGTTCTTGAGAAGAAGAGAAACAAC TGTTTTGTTGAATCTGGCCAACCTATTTGTGGAAATGGAATGGTAGAACAAGGTGAAGAA TGTGATTGTGGCTATAGTGACCAGTGTAAAGATGAATGCTGCTTCGATGCAAATCAACCA GAGGGAAGAAAATGCAAACTGAAACCTGGGAAACAGTGCAGTCCAAGTCAAGGTCCTTGT TGTACAGCACAGTGTGCATTCAAGTCAAAGTCTGAGAAGTGTCGGGATGATTCAGACTGT GCAAGGGAAGGAATATGTAATGGCTTCACAGCTCTCTGCCCAGCATCTGACCCTAAACCA AACTTCACAGACTGTAATAGGCATACACAAGTGTGCATTAATGGGCAATGTGCAGGTTCT ATCTGTGAGAAATATGGCTTAGAGGAGTGTACGTGTGCCAGTTCTGATGGCAAAGATGAT AAAGAATTATGCCATGTATGCTGTATGAAGAAAATGGACCCATCAACTTGTGCCAGTACA GGGTCTGTGCAGTGGAGTAGGCACTTCAGTGGTCGAACCATCACCCTGCAACCTGGATCC CCTTGCAACGATTTTAGAGGTTACTGTGATGTTTTCATGCGGTGCAGATTAGTAGATGCT GATGGTCCTCTAGCTAGGCTTAAAAAAGCAATTTTTAGTCCAGAGCTCTATGAAAACATT GCTGAATGGATTGTGGCTCATTGGTGGGCAGTATTACTTATGGGAATTGCTCTGATCATG CTAATGGCTGGATTTATTAAGATATGCAGTGTTCATACTCCAAGTAGTAATCCAAAGTTG CCTCCTCCTAAACCACTTCCAGGCACTTTAAAGAGGAGGAGACCTCCACAGCCCATTCAG CAACCCCAGCGTCAGCGGCCCCGAGAGAGTTATCAAATGGGACACATGAGACGCTAACTG CAGCTTTTGCCTTGGTTCTTCCTAGTGCCTACAATGGGAAAACTTCACTCCAAAGAGAAA CCTATTAAGTCATCATCTCCAAACTAAACCCTCACAAGTAACAGTTGAAGAAAAAATGGC AAGAGATCATATCCTCAGACCAGGTGGAATTACTTAAATTTTAAAGCCTGAAAATTCCAA TTTGGGGGTGGGAGGTGGAAAAGGAACCCAATTTTCTTATGAACAGATATTTTTAACTTA ATGGCACAAAGTCTTAGAATATTATTATGTGCCCCGTGTTCCCTGTTCTTCGTTGCTGCA TTTTCTTCACTTGCAGGCAAACTTGGCTCTCAATAAACTTTTACCACAAATTGAAATAAA TATATTTTTTTCAACTGCC >OriGene 5' read for NM_001110 unedited
NGGTTCAAATTTCGGATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCGTTTC TCCTGCCAGGGAGGTCCCGGCTTCCCGTGGAGGCTCCGGACCAAGCCCCTTCAGCTTCTC CCTCCGGATCGATGTGCTGCTGTTAACCCGTGAGGAGGCGGCGGCGGCGGCAGCGGCAGC GGAAGATGGTGTTGCTGAGAGTGTTAATTCTGCTCCTCTCCTGGGCGGCGGGGATGGGAG GTCAGTATGGGAATCCTTTAAATAAATATATCAGACATTATGAAGGATTATCTTACAATG TGGATTCATTACACCAAAAACACCAGCGTGCCAAAAGAGCAGTCTCACATGAAGACCAAT TTTTACGTCTAGATTTCCATGCCCATGGAAGACATTTCAACCTACGAATGAAGAGGGACA CTTCCCTTTTCAGTGATGAATTTAAAGTAGAAACATCAAATAAAGTACTTGATTATGATA CCTCTCATATTTACACTGGACATATTTATGGTGAAGAAGGAAGTTTTAGCCATGGGTCTG TTATTGATGGAAGATTTGAAGGATTCATCCAGACTCGTGGTGGCACATTTTATGTTGAGC CAGCAGAGAGATATATTAAAGACCGAACTCTGCCATTTCACTCTGTCATTTATCATGAAG ATGATATTAACTATCCCCATAAATACGGTCCTCANGGGGGCTGTGCAGATCATTCAGTAT TTGAAAGAATGAGGAAATACCAGATGACTGGTGTAGAGGAAAGTACACAGATACCTCAAG AAGACATGCTGCNTATGGTCCAGAACTTNCTGAGAAAAACGTACACTTCAGCTGAAAAAA TACTTGTCAGCTNTATATTCAGACTGATCATTTGTTCCTTTAATATTACGGGACACCGAA A |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001110 |
| Insert Size | 4760 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001110.2, NP_001101.1 |
| RefSeq Size | 3927 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 102 |
| UniProt ID | O14672 |
| Domains | Reprolysin, DISIN |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Protein Pathways | Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection |
| Gene Summary | Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Modification of proteolytic activity matrix analysis (PrAMA) to measure ADAM10 and ADAM17 sheddase activities in cell and tissue lysates
,Yoneyama, T;Gorry, M;Miller, MA;Gaither-Davis, A;Lin, Y;Moss, ML;Griffith, LG;Lauffenburger, DA;Stabile, LP;Herman, JG;Vujanovic, NL;,
J Cancer
,PubMed ID 29187866
[ADAM10]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215457 | ADAM10 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 10 (ADAM10) |
CNY 8,216.00 |
|
| RC215457L1 | Lenti-ORF clone of ADAM10 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 10 (ADAM10) |
CNY 7,700.00 |
|
| RC215457L2 | Lenti-ORF clone of ADAM10 (mGFP-tagged)-Human ADAM metallopeptidase domain 10 (ADAM10) |
CNY 7,700.00 |
|
| RC215457L3 | Lenti-ORF clone of ADAM10 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 10 (ADAM10) |
CNY 7,700.00 |
|
| RC215457L4 | Lenti-ORF clone of ADAM10 (mGFP-tagged)-Human ADAM metallopeptidase domain 10 (ADAM10) |
CNY 10,616.00 |
|
| RG215457 | ADAM10 (tGFP-tagged) - Human ADAM metallopeptidase domain 10 (ADAM10) |
CNY 9,816.00 |
