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Bak (BAK1) (NM_001188) Human Untagged Clone
CAT＃: SC119385
BAK1 (untagged)-Human BCL2-antagonist/killer 1 (BAK1)
CNY 3,600.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BAK; BAK-LIKE; BCL2L7; CDN1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC119385 representing NM_001188.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCGGCCGCGAATTCGGCACGAGGCACAGGGACAAGTAAAGGCTACATCCAGATGCCGGGAATGCACTGA CGCCCATTCCTGGAAACTGGGCTCCCACTCAGCCCCTGGGAGCAGCAGCCGCCAGCCCCTCGGGACCTC CATCTCCACCCTGCTGAGCCACCCGGGTTGGGCCAGGATCCCGGCAGGCTGATCCCGTCCTCCACTGAG ACCTGAAAAATGGCTTCGGGGCAAGGCCCAGGTCCTCCCAGGCAGGAGTGCGGAGAGCCTGCCCTGCCC TCTGCTTCTGAGGAGCAGGTAGCCCAGGACACAGAGGAGGTTTTCCGCAGCTACGTTTTTTACCGCCAT CAGCAGGAACAGGAGGCTGAAGGGGTGGCTGCCCCTGCCGACCCAGAGATGGTCACCTTACCTCTGCAA CCTAGCAGCACCATGGGGCAGGTGGGACGGCAGCTCGCCATCATCGGGGACGACATCAACCGACGCTAT GACTCAGAGTTCCAGACCATGTTGCAGCACCTGCAGCCCACGGCAGAGAATGCCTATGAGTACTTCACC AAGATTGCCACCAGCCTGTTTGAGAGTGGCATCAATTGGGGCCGTGTGGTGGCTCTTCTGGGCTTCGGC TACCGTCTGGCCCTACACGTCTACCAGCATGGCCTGACTGGCTTCCTAGGCCAGGTGACCCGCTTCGTG GTCGACTTCATGCTGCATCACTGCATTGCCCGGTGGATTGCACAGAGGGGTGGCTGGGTGGCAGCCCTG AACTTGGGCAATGGTCCCATCCTGAACGTGCTGGTGGTTCTGGGTGTGGTTCTGTTGGGCCAGTTTGTG GTACGAAGATTCTTCAAATCATGACTCCCAAGGGTGCCCTTTGGGGTCCCGGTTCAGACCCCTGCCTGG ACTTAAGCGAAGTCTTTGCCTTCTCTGCTCCCTTGCAGGGGTCCCCCCTCAAGAGTACAGAAGCTTTAG CAAGTGTGCACTCCAGCTTCGGAGGGCCCCTGCGTGGGGGCCAGTCAGGCTGCAGAGGCACCTCAACAT TGCATGGTGCTAGTGGGCCCTCTCTCTGGGCCCAGGGGCTGTGGCCGTCTCCTCCCTCAGCTCTCTGGG ACCTCCTTAGCCCTGTCTGCTAGGCGCTGGGGAGACTGATAACTTGGGGAGGCAAGAGACTGGGAGCCA CTTCTCCCCAGAAAGTGTTTAACGGTTTTAGCTTTTTATAATACCCTTGTGAGAGCCCATTCCCACCAT TCTACCTGAGGCCAGGACGTCTGGGGTGTGGGGATTGGTGGGTCTATGTTCCCCAGGATTCAGCTATTC TGGAAGATCAGCACCCTAAGAGATGGGACTAGGACCTGAGCCTGGTCCTGGCCGTCCCTAAGCATGTGT CCCAGGAGCAGGACCTACTAGGAGAGGGGGGCCAAGGTCCTGCTCAACTCTACCCCTGCTCCCATTCCT CCCTCCGGCCATACTGCCTTTGCAGTTGGACTCTCAGGGATTCTGGGCTTGGGGTGTGGGGTGGGGTGG AGTCGCAGACCAGAGCTGTCTGAACTCACGTGTCAGAAGCCTCCAAGCCTGCCTCCCAAGGTCCTCTCA GTTCTCTCCCTTCCTCTCTCCTTATAGACACTTGCTCCCAACCCATTCACTACAGGTGAAGGCTCTCAC CCCCATCCCTGGGGGCCTTGGGTGAGTGGCCTGCTAAGGCTCCTCCTTGCCCAGACTACAGGGCTTAGG ACTTGGTTTGTTATTTCAGGGAAAAGGAGTAGGGAGTTCATCTGGAGGGTTCTAAGTGGGAGAAGGACT ATCAACACCACTAGGAATCCCAGAGGTGGGATCCTCCCTCATGGCTCTGGCACAGTGTAATCCAGGGGT GTAGATGGGGGAACTGTGAATACTTGAACTCTGTTCCCCCACCCTCCATGCTCCTCACCTGTCTAGGTC TCCTCAGGGTGGGGGGTGACAGTGCCTTCTCTATTGGGCACAGCCTAGGGTCTTGGGGGTCAGGGGGGA GAAGTTCTTGATTCAGCCAAATGCAGGGAGGGGAGGCAGATGGAGCCCATAGGCCACCCCCTATCCTCT GAGTGTTTGGAAATAAACTGTGCAATCCCCTCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGC CGC >OriGene 5' read for NM_001188 unedited
GGTTATAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCACAGG GACAAGTAAAGGCTACATCCAGATGCCGGGAATGCACTGACGCCCATTCCTGGAAACTGG GCTCCCACTCAGCCCCTGGGAGCAGCAGCCGCCAGCCCCTCGGGACCTCCATCTCCACCC TGCTGAGCCACCCGGGTTGGGCCAGGATCCCGGCAGGCTGATCCCGTCCTCCACTGAGAC CTGAAAAATGGCTTCGGGGCAAGGCCCAGGTCCTCCCAGGCAGGAGTGCGGAGAGCCTGC CCTGCCCTCTGCTTCTGAGGAGCAGGTAGCCCAGGACACAGAGGAGGTTTTCCGCAGCTA CGTTTTTTACCGCCATCAGCAGGAACAGGAGGCTGAAGGGGTGGCTGCCCCTGCCGACCC AGAGATGGTCACCTTACCTCTGCAACCTAGCAGCACCATGGGGCAGGTGGGACGGCAGCT CGCCATCATCGGGGACGACATCAACCGACGCTATGACTCAGAGTTCCAGACCATGTTGCA GCACCTGCAGCCCACGGCAGAGAATGCCTATGAGTACTTCACCAAGATTGCCACCAGCCT GTTTGAGAGTGGCATCAATTGGGGCCGTGTGGTGGCTCTTCTGGGCTTCGGCTACCGTCT GGCCCTACACGTCTACCAGCATGCCCTGACTGGCTTCCTANGCCANGTGACCCGCTTCGT GGTCGACTTCATGCTGCATCACTGCATTGCCCGGTGGATTGCACACAGGGCTGCCTGGGT GGCAGCCCTGAACTTTGGCCATGGTCCCATCCTGAACGTGCTGGTGGNTCCTGGGGTGGG TCTTGTGGGCCCAGTTGTGTTACGAAAAATCTCAAACATGACTCCCAAGGTGCCCTTTGG GGTCCCGGTCAGACCCCA >OriGene 3' read for NM_001188 unedited
ATGGCCGCGGCCCGCATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAGGGGATTGCACA GTTTATTTCCAAACACTCAGNAGATAGGGGGTGGCCTATGGGCTCCATCTGCCTCCCCTC CCTGCATTTGGCTGAATCAAGAACTTCTCCCCCCTGACCCCCAAGACCCTAGGCTGTGCC CAATAGAGAAGGCACTGTCACCCCCCACCCTGAGGAGACCTAGACAGGTGAGGAGCATGG AGGGTGGGGGAACAGAGTTCAAGTATTCACAGTTCCCCCATCTACACCCCTGGATTACAC TGTGCCAGAGCCATGAGGGAGGATCCCACCTCTGGGATTCCTAGTGGCGTTGATAGTCCT TCTCCCACTTAGAACCCTCCAGATGAACTCCCTACTCCTTTTCCCTGAAATAACAAACCA AGTCCTAAGCCCTGTAGTCTGGGCAAGGAGGAGCCTTAGCAGGCCACTCACCCAAGGCCC CCAGGGATGGGGGTGAGAGCCCTTCACCTGTAGTGAATGGGTTGGGAGCAAGTGTCTATA AGGAGAGAGGAAGGGAGAGAACTGACAGGACCTTGGGAGGCAGGCTTGGAGGCTTCTGAC ACGTGAGTTCAGACAGCTCTGGTCTGCGACTTCACCCCACCCACACCCCAAGCCCAGAAT CCCTGAGAGTTCAACTGCAAAGGCCATATGGCCGGAGGGAGGAATGGCAGCCAGGGTAAA GTTGACCAGGACCTTGATCACCCTTTACTAAAGGACCTGCTCCTGGGCACATGCTTAGGG ACGCCCAGCACCAGCCTCAGCTCCTAGACCCATCTCTTAGGGGCTGATCTTACAAAAACC TGAAATCTGGGGAACATAAACCCCCCATTTCCGACCCCCCGAAGACCTGGACCCAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001188 |
| Insert Size | 2142 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001188.2 |
| RefSeq Size | 2165 bp |
| RefSeq ORF | 2142 bp |
| Locus ID | 578 |
| UniProt ID | Q16611 |
| Domains | Bcl-2 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transmembrane |
| MW | 77.1 kDa |
| Gene Summary | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Notch4 signaling confers susceptibility to TRAIL induced apoptosis in breast cancer cells
,Naik, S;MacFarlane, M;Sarin, A;,
J. Cell. Biochem.
,PubMed ID 25704336
[BAK1]
|
|
The C-terminal domain (CTD) in linker histones antagonizes anti-apoptotic proteins to modulate apoptotic outcomes at the mitochondrion
,Garg, M;Ramdas, N;Vijayalakshmi, M;Shivashankar, GV;Sarin, A;,
Cell Death Dis, Feb 2014.
,PubMed ID 24525734
[BAK1]
|
|
Sorafenib Sensitizes (–)-Gossypol-Induced Growth Suppression in Androgen-Independent Prostate Cancer Cells via Mcl-1 Inhibition and Bak Activation
,Jiqin Lian, Zhenhong Ni, Xufang Dai, Chang Su, Amber Rae Smith, Liang Xu, and Fengtian He,
Mol. Cancer Ther., Feb 2012; 11: 416 - 426.
[BAK1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201216 | BAK1 (Myc-DDK-tagged)-Human BCL2-antagonist/killer 1 (BAK1) |
CNY 3,600.00 |
|
| RC201216L1 | Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201216L2 | Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), mGFP tagged |
CNY 5,890.00 |
|
| RC201216L3 | Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201216L4 | Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), mGFP tagged |
CNY 5,890.00 |
|
| RG201216 | BAK1 (tGFP-tagged) - Human BCL2-antagonist/killer 1 (BAK1) |
CNY 5,200.00 |
