RhoGAP (ARHGAP5) (NM_001173) Human Untagged Clone
CAT#: SC119374
ARHGAP5 (untagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2
CNY 10,280.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GFI2; p190-B; p190BRhoGAP; RhoGAP5 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001173, the custom clone sequence may differ by one or more nucleotides
ATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGACTCTCTGGGACTG AAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAATAGATTTGTACGCTCAAAAGCAGA TGAATATTATCCAGAGCATACTTCTGTGCTTAGCACCATTGACTTTGGAGGACGAGTAGTAAACAATGAT CACTTTTTGTACTGGGGTGACATAATACAAAATAGTGAAGATGGAGTAGAATGCAAAATTCATGTCATTG AACAAACAGAGTTCATTGATGACCAGACTTTCTTGCCTCATCGGAGTACGAATTTGCAACCATATATAAA ACGTGCAGCTGCATCTAAATTGCAGTCAGCAGAAAAACTAATGTACATTTGCACTGATCAGCTAGGCTTA GAACAAGACTTTGAACAGAAGCAAATGCCTGAAGGGAAGCTCAACGTAGATGGATTTTTATTATGCATTG ATGTAAGTCAAGGATGCAATAGGAAGTTTGATGATCAACTTAAATTTGTGAATAACCTTTTTGTCCAGTT ATCAAAATCAAAAAAACCTGTAATAATAGCAGCAACTAAATGTGATGAATGCGTGGATCATTATCTTAGA GAAGTTCAGGCATTTGCTTCAAATAAAAAGAACCTTCTTGTAGTGGAAACATCAGCACGATTTAATGTCA ACATTGAAACATGTTTTACTGCACTGGTACAAATGTTGGATAAAACTCGTAGCAAGCCTAAAATTATTCC CTATTTGGATGCTTATAAAACACAGAGACAACTTGTTGTCACAGCAACAGATAAGTTTGAAAAACTTGTG CAGACTGTGAGAGATTATCATGCAACTTGGAAAACTGTTAGTAATAAATTAAAAAATCATCCTGATTATG AAGAATACATCAACTTAGAGGGAACAAGAAAGGCCAGAAATACATTCTCAAAACATATAGAACAACTTAA ACAGGAACATATAAGAAAAAGGAGAGAAGAGTATATAAATACTTTACCAAGAGCTTTTAACACTCTTTTG CCAAATCTAGAAGAGATTGAACATTTGAATTGGTCAGAAGCTTTGAAGTTAATGGAAAAGAGAGCAGATT TCCAGTTATGTTTTGTGGTGCTAGAAAAAACTCCTTGGGATGAAACTGACCATATAGACAAAATTAATGA TAGGCGGATTCCATTTGACCTCCTGAGCACTTTAGAAGCTGAAAAAGTCTATCAGAACCATGTACAGCAT CTGATATCCGAGAAGAGGAGGGTGGAAATGAAGGAAAAATTCAAAAAGACTTTGGAAAAAATTCAATTCA TTTCACCAGGGCAGCCATGGGAGGAAGTTATGTGCTTTGTTATGGAGGATGAAGCCTACAAATATATCAC TGAGGCTGATAGCAAAGAGGTATATGGTAGGCATCAGCGAGAAATAGTTGAAAAAGCCAAAGAAGAGTTT CAAGAAATGCTTTTTGAGCATTCTGAACTTTTTTATGATTTAGATCTTAATGCAACACCTAGTTCAGATA AAATGAGTGAAATTCATACAGTTCTGAGTGAAGAACCTAGATATAAAGCTTTACAGAAACTTGCACCTGA TAGGGAATCCCTTCTACTTAAGCATATAGGATTTGTTTATCATCCCACTAAAGAAACATGTCTTAGTGGC CAAAATTGTACAGACATTAAAGTGGAGCAGTTACTTGCTAGTAGTCTTTTACAGTTGGATCATGGCCGCT TAAGATTATATCACGATAGTACCAATATAGATAAAGTTAACCTTTTTATTTTAGGGAAGGATGGCCTTGC CCAAGAACTAGCAAATGAGATAAGGACACAATCCACTGATGATGAGTATGCCTTAGATGGAAAAATTTAT GAACTTGATCTTCGGCCGGTTGATGCCAAATCGCCTTACTTTTTGAGTCAGTTATGGACTGCCGCCTTTA AACCACATGGGTGCTTCTGTGTATTTAATTCCATTGAGTCATTGAGTTTTATTGGGGAATTTATTGGGAA AATAAGAACTGAAGCTTCTCAGATCAGAAAAGATAAATACATGGCTAATCTTCCATTTACATTAATTCTG GCTAATCAGAGAGATTCCATTAGTAAGAATCTACCAATTCTCAGGCACCAAGGGCAGCAGTTGGCAAACA AGTTGCAATGTCCTTTTGTAGATGTACCTGCTGGTACATATCCTCGTAAATTTAATGAAACCCAAATAAA GCAAGCTCTCAGAGGAGTATTGGAATCAGTTAAACACAATTTGGATGTGGTGAGCCCAATTCCTGCCAAT AAGGACTTATCAGAAGCTGACTTGAGAATTGTCATGTGCGCCATGTGTGGAGATCCATTTAGTGTGGATC TTATTCTTTCACCCTTCCTTGATTCTCATTCTTGCAGTGCTGCTCAAGCTGGACAGAATAATTCCCTAAT GCTTGATAAAATCATTGGTGAAAAAAGGAGGCGAATACAGATCACAATATTATCATACCACTCTTCAATT GGAGTAAGAAAAGATGAACTAGTTCATGGGTATATATTAGTTTACTCTGCAAAACGGAAAGCTTCGATGG GAATGCTTCGAGCATTTCTATCAGAAGTTCAAGACACCATTCCTGTACAGCTGGTGGCAGTTACTGACAG CCAAGCAGATTTTTTTGAAAATGAGGCTATCAAAGAGTTAATGACTGAAGGAGAACACATTGCAACTGAG ATCACTGCTAAATTTACAGCACTGTATTCTTTATCTCAGTATCATCGGCAAACTGAGGTCTTTACTCTGT TTTTTAGTGATGTTCTAGAGAAAAAAAATATGATAGAAAATTCTTATTTGTCTGATAATACAAGGGAATC AACCCATCAAAGTGAAGATGTTTTTCTACCATCTCCCAGAGACTGTTTTCCCTATAATAACTACCCTGAT TCAGATGATGACACAGAAGCACCACCTCCTTATAGTCCAATTGGGGATGATGTACAGTTGCTTCCAACAC CTAGTGACCGTTCCAGATATAGATTAGATTTGGAAGGAAATGAGTATCCTATTCATAGTACCCCAAACTG TCATGACCATGAACGCAACCATAAAGTGCCTCCACCTATTAAACCTAAACCAGTTGTACCTAAGACAAAT GTGAAAAAACTCGATCCAAACCTTTTAAAAACAATTGAAGCTGGTATTGGTAAAAATCCAAGAAAGCAGA CTTCCCGGGTGCCTTTGGCACATCCTGAAGATATGGATCCTTCAGATAACTATGCGGAACCCATTGATAC AATTTTCAAACAGAAGGGCTATTCTGATGAGATTTATGTTGTCCCAGATGATAGTCAAAATCGTATTAAA ATTCGAAACTCATTTGTAAATAACACCCAAGGAGATGAAGAAAATGGGTTTTCTGATAGAACCTCAAAAA GTCATGGGGAACGGAGGCCTTCAAAATACAAATATAAATCTAAAACCTTGTTTAGTAAAGCCAAGTCATA CTATAGAAGAACACATTCAGATGCCAGTGATGATGAGGCTTTCACCACTTCTAAAACAAAAAGAAAAGGA AGACATCGTGGAAGTGAAGAAGATCCACTTCTTTCTCCTGTTGAAACTTGGAAAGGTGGTATTGATAATC CTGCAATCACTTCTGACCAGGAGTTAGATGATAAGAAGATGAAGAAGAAAACCCACAAAGTGAAAGAAGA TAAAAAGAAAAAGAAAACTAAGAACTTCAATCCACCAACACGTAGAAATTGGGAAAGTAATTACTTTGGG ATGCCCCTCCAGGATCTGGTTACAGCTGAGAAGCCCATACCACTATTTGTTGAGAAATGTGTGGAATTTA TTGAAGATACAGGGTTATGTACCGAAGGACTCTACCGTGTCAGCGGGAATAAAACTGACCAAGACAATAT TCAAAAGCAGTTTGATCAAGATCATAATATCAATCTAGTGTCAATGGAAGTAACAGTAAATGCTGTAGCT GGAGCCCTTAAAGCTTTCTTTGCAGATCTGCCAGATCCTTTAATTCCATATTCTCTTCATCCAGAACTAT TGGAAGCAGCAAAAATCCCGGATAAAACAGAACGTCTTCATGCCTTGAAAGAAATTGTTAAGAAATTTCA TCCTGTAAACTATGATGTATTCAGATACGTGATAACACATCTAAACAGGGTTAGTCAGCAACATAAAATC AACCTAATGACAGCAGACAACTTATCCATCTGTTTTTGGCCAACCTTGATGAGACCTGATTTTGAAAATC GAGAGTTTCTGTCTACTACTAAGATTCATCAATCTGTTGTTGAAACATTCATTCAGCAGTGTCAGTTTTT CTTTTACAATGGAGAAATTGTAGAAACGACAAACATTGTGGCTCCTCCACCACCTTCAAACCCAGGACAG TTGGTGGAACCAATGGTGCCACTTCAGTTGCCGCCACCATTGCAACCTCAGCTGATACAACCACAATTAC AAACGGATCCTCTTGGTATTATATGA >OriGene 5' read for NM_001173 unedited
CGGCCGGCGATTCGGCACCAGGAGAGTGGAGGAGGAGGCCGCGGCGGCGGGAGCGGTCCC CAGGAATGTCGCTGCCGCCGCCACCGCCGGGGCCGCTGCCGTTGAGGAGGAGACGGAGGA GACCGACGTTGTTAGGAAGATGATCCCTATGATCTTGAAGATGTTTCTGCACAGAAATGA GGGAAATACAAAGAACCAAATACAGTTCTGAAATTTGGGATCTGTATTTTGAGATGATTT TATTTTCAGAATGAGAAGCATATCTGGTTACCTTTATGAATGTAGAGACATGAGAAGAGA GTTATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGA CTCTCTGGGACTGAAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAAT AGATTTGTACGCTCAAAAGCAGATGAATATTATCCAGAGCATACTTCTGTGCTTAGCACC ATTGACTCTGGAGGACGAGTATCAAACAATGATCACTTTTTGTCTGGGCGTGCATAATAC AAAATAGTGAAGATGGAGTACAATGCAACATCATGTCATTGAACAACAGACGTCATTGAT GACCACACTTTCTTGCCTATCCGGATACGAATTTGAACCATATATAAAACGTGCAGTCGC ATCTAATCGCACCAGCATAAAACTTATGAACTTTGCACTGATCACCC |
Restriction Sites | NotI-NotI |
ACCN | NM_001173 |
Insert Size | 5000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001173.2, NP_001164.2 |
RefSeq Size | 9601 bp |
RefSeq ORF | 4506 bp |
Locus ID | 394 |
UniProt ID | Q13017 |
Domains | RhoGAP, FF |
Protein Pathways | Focal adhesion, Leukocyte transendothelial migration |
Gene Summary | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is one aa shorter compared to isoform a. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217131 | ARHGAP5 (Myc-DDK-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 10,272.00 |
|
RC217131L3 | Lenti-ORF clone of ARHGAP5 (Myc-DDK-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 16,910.00 |
|
RC217131L4 | Lenti-ORF clone of ARHGAP5 (mGFP-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 16,910.00 |
|
RG217131 | ARHGAP5 (tGFP-tagged) - Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 11,872.00 |