RhoGAP (ARHGAP5) (NM_001173) Human Untagged Clone

CAT#: SC119374

ARHGAP5 (untagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2



  "NM_001173" in other vectors (4)

CNY 10,280.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ARHGAP5 mouse monoclonal antibody,clone OTI9H2
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GFI2; p190-B; p190BRhoGAP; RhoGAP5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001173, the custom clone sequence may differ by one or more nucleotides


ATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGACTCTCTGGGACTG
AAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAATAGATTTGTACGCTCAAAAGCAGA
TGAATATTATCCAGAGCATACTTCTGTGCTTAGCACCATTGACTTTGGAGGACGAGTAGTAAACAATGAT
CACTTTTTGTACTGGGGTGACATAATACAAAATAGTGAAGATGGAGTAGAATGCAAAATTCATGTCATTG
AACAAACAGAGTTCATTGATGACCAGACTTTCTTGCCTCATCGGAGTACGAATTTGCAACCATATATAAA
ACGTGCAGCTGCATCTAAATTGCAGTCAGCAGAAAAACTAATGTACATTTGCACTGATCAGCTAGGCTTA
GAACAAGACTTTGAACAGAAGCAAATGCCTGAAGGGAAGCTCAACGTAGATGGATTTTTATTATGCATTG
ATGTAAGTCAAGGATGCAATAGGAAGTTTGATGATCAACTTAAATTTGTGAATAACCTTTTTGTCCAGTT
ATCAAAATCAAAAAAACCTGTAATAATAGCAGCAACTAAATGTGATGAATGCGTGGATCATTATCTTAGA
GAAGTTCAGGCATTTGCTTCAAATAAAAAGAACCTTCTTGTAGTGGAAACATCAGCACGATTTAATGTCA
ACATTGAAACATGTTTTACTGCACTGGTACAAATGTTGGATAAAACTCGTAGCAAGCCTAAAATTATTCC
CTATTTGGATGCTTATAAAACACAGAGACAACTTGTTGTCACAGCAACAGATAAGTTTGAAAAACTTGTG
CAGACTGTGAGAGATTATCATGCAACTTGGAAAACTGTTAGTAATAAATTAAAAAATCATCCTGATTATG
AAGAATACATCAACTTAGAGGGAACAAGAAAGGCCAGAAATACATTCTCAAAACATATAGAACAACTTAA
ACAGGAACATATAAGAAAAAGGAGAGAAGAGTATATAAATACTTTACCAAGAGCTTTTAACACTCTTTTG
CCAAATCTAGAAGAGATTGAACATTTGAATTGGTCAGAAGCTTTGAAGTTAATGGAAAAGAGAGCAGATT
TCCAGTTATGTTTTGTGGTGCTAGAAAAAACTCCTTGGGATGAAACTGACCATATAGACAAAATTAATGA
TAGGCGGATTCCATTTGACCTCCTGAGCACTTTAGAAGCTGAAAAAGTCTATCAGAACCATGTACAGCAT
CTGATATCCGAGAAGAGGAGGGTGGAAATGAAGGAAAAATTCAAAAAGACTTTGGAAAAAATTCAATTCA
TTTCACCAGGGCAGCCATGGGAGGAAGTTATGTGCTTTGTTATGGAGGATGAAGCCTACAAATATATCAC
TGAGGCTGATAGCAAAGAGGTATATGGTAGGCATCAGCGAGAAATAGTTGAAAAAGCCAAAGAAGAGTTT
CAAGAAATGCTTTTTGAGCATTCTGAACTTTTTTATGATTTAGATCTTAATGCAACACCTAGTTCAGATA
AAATGAGTGAAATTCATACAGTTCTGAGTGAAGAACCTAGATATAAAGCTTTACAGAAACTTGCACCTGA
TAGGGAATCCCTTCTACTTAAGCATATAGGATTTGTTTATCATCCCACTAAAGAAACATGTCTTAGTGGC
CAAAATTGTACAGACATTAAAGTGGAGCAGTTACTTGCTAGTAGTCTTTTACAGTTGGATCATGGCCGCT
TAAGATTATATCACGATAGTACCAATATAGATAAAGTTAACCTTTTTATTTTAGGGAAGGATGGCCTTGC
CCAAGAACTAGCAAATGAGATAAGGACACAATCCACTGATGATGAGTATGCCTTAGATGGAAAAATTTAT
GAACTTGATCTTCGGCCGGTTGATGCCAAATCGCCTTACTTTTTGAGTCAGTTATGGACTGCCGCCTTTA
AACCACATGGGTGCTTCTGTGTATTTAATTCCATTGAGTCATTGAGTTTTATTGGGGAATTTATTGGGAA
AATAAGAACTGAAGCTTCTCAGATCAGAAAAGATAAATACATGGCTAATCTTCCATTTACATTAATTCTG
GCTAATCAGAGAGATTCCATTAGTAAGAATCTACCAATTCTCAGGCACCAAGGGCAGCAGTTGGCAAACA
AGTTGCAATGTCCTTTTGTAGATGTACCTGCTGGTACATATCCTCGTAAATTTAATGAAACCCAAATAAA
GCAAGCTCTCAGAGGAGTATTGGAATCAGTTAAACACAATTTGGATGTGGTGAGCCCAATTCCTGCCAAT
AAGGACTTATCAGAAGCTGACTTGAGAATTGTCATGTGCGCCATGTGTGGAGATCCATTTAGTGTGGATC
TTATTCTTTCACCCTTCCTTGATTCTCATTCTTGCAGTGCTGCTCAAGCTGGACAGAATAATTCCCTAAT
GCTTGATAAAATCATTGGTGAAAAAAGGAGGCGAATACAGATCACAATATTATCATACCACTCTTCAATT
GGAGTAAGAAAAGATGAACTAGTTCATGGGTATATATTAGTTTACTCTGCAAAACGGAAAGCTTCGATGG
GAATGCTTCGAGCATTTCTATCAGAAGTTCAAGACACCATTCCTGTACAGCTGGTGGCAGTTACTGACAG
CCAAGCAGATTTTTTTGAAAATGAGGCTATCAAAGAGTTAATGACTGAAGGAGAACACATTGCAACTGAG
ATCACTGCTAAATTTACAGCACTGTATTCTTTATCTCAGTATCATCGGCAAACTGAGGTCTTTACTCTGT
TTTTTAGTGATGTTCTAGAGAAAAAAAATATGATAGAAAATTCTTATTTGTCTGATAATACAAGGGAATC
AACCCATCAAAGTGAAGATGTTTTTCTACCATCTCCCAGAGACTGTTTTCCCTATAATAACTACCCTGAT
TCAGATGATGACACAGAAGCACCACCTCCTTATAGTCCAATTGGGGATGATGTACAGTTGCTTCCAACAC
CTAGTGACCGTTCCAGATATAGATTAGATTTGGAAGGAAATGAGTATCCTATTCATAGTACCCCAAACTG
TCATGACCATGAACGCAACCATAAAGTGCCTCCACCTATTAAACCTAAACCAGTTGTACCTAAGACAAAT
GTGAAAAAACTCGATCCAAACCTTTTAAAAACAATTGAAGCTGGTATTGGTAAAAATCCAAGAAAGCAGA
CTTCCCGGGTGCCTTTGGCACATCCTGAAGATATGGATCCTTCAGATAACTATGCGGAACCCATTGATAC
AATTTTCAAACAGAAGGGCTATTCTGATGAGATTTATGTTGTCCCAGATGATAGTCAAAATCGTATTAAA
ATTCGAAACTCATTTGTAAATAACACCCAAGGAGATGAAGAAAATGGGTTTTCTGATAGAACCTCAAAAA
GTCATGGGGAACGGAGGCCTTCAAAATACAAATATAAATCTAAAACCTTGTTTAGTAAAGCCAAGTCATA
CTATAGAAGAACACATTCAGATGCCAGTGATGATGAGGCTTTCACCACTTCTAAAACAAAAAGAAAAGGA
AGACATCGTGGAAGTGAAGAAGATCCACTTCTTTCTCCTGTTGAAACTTGGAAAGGTGGTATTGATAATC
CTGCAATCACTTCTGACCAGGAGTTAGATGATAAGAAGATGAAGAAGAAAACCCACAAAGTGAAAGAAGA
TAAAAAGAAAAAGAAAACTAAGAACTTCAATCCACCAACACGTAGAAATTGGGAAAGTAATTACTTTGGG
ATGCCCCTCCAGGATCTGGTTACAGCTGAGAAGCCCATACCACTATTTGTTGAGAAATGTGTGGAATTTA
TTGAAGATACAGGGTTATGTACCGAAGGACTCTACCGTGTCAGCGGGAATAAAACTGACCAAGACAATAT
TCAAAAGCAGTTTGATCAAGATCATAATATCAATCTAGTGTCAATGGAAGTAACAGTAAATGCTGTAGCT
GGAGCCCTTAAAGCTTTCTTTGCAGATCTGCCAGATCCTTTAATTCCATATTCTCTTCATCCAGAACTAT
TGGAAGCAGCAAAAATCCCGGATAAAACAGAACGTCTTCATGCCTTGAAAGAAATTGTTAAGAAATTTCA
TCCTGTAAACTATGATGTATTCAGATACGTGATAACACATCTAAACAGGGTTAGTCAGCAACATAAAATC
AACCTAATGACAGCAGACAACTTATCCATCTGTTTTTGGCCAACCTTGATGAGACCTGATTTTGAAAATC
GAGAGTTTCTGTCTACTACTAAGATTCATCAATCTGTTGTTGAAACATTCATTCAGCAGTGTCAGTTTTT
CTTTTACAATGGAGAAATTGTAGAAACGACAAACATTGTGGCTCCTCCACCACCTTCAAACCCAGGACAG
TTGGTGGAACCAATGGTGCCACTTCAGTTGCCGCCACCATTGCAACCTCAGCTGATACAACCACAATTAC
AAACGGATCCTCTTGGTATTATATGA


>OriGene 5' read for NM_001173 unedited
CGGCCGGCGATTCGGCACCAGGAGAGTGGAGGAGGAGGCCGCGGCGGCGGGAGCGGTCCC
CAGGAATGTCGCTGCCGCCGCCACCGCCGGGGCCGCTGCCGTTGAGGAGGAGACGGAGGA
GACCGACGTTGTTAGGAAGATGATCCCTATGATCTTGAAGATGTTTCTGCACAGAAATGA
GGGAAATACAAAGAACCAAATACAGTTCTGAAATTTGGGATCTGTATTTTGAGATGATTT
TATTTTCAGAATGAGAAGCATATCTGGTTACCTTTATGAATGTAGAGACATGAGAAGAGA
GTTATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGA
CTCTCTGGGACTGAAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAAT
AGATTTGTACGCTCAAAAGCAGATGAATATTATCCAGAGCATACTTCTGTGCTTAGCACC
ATTGACTCTGGAGGACGAGTATCAAACAATGATCACTTTTTGTCTGGGCGTGCATAATAC
AAAATAGTGAAGATGGAGTACAATGCAACATCATGTCATTGAACAACAGACGTCATTGAT
GACCACACTTTCTTGCCTATCCGGATACGAATTTGAACCATATATAAAACGTGCAGTCGC
ATCTAATCGCACCAGCATAAAACTTATGAACTTTGCACTGATCACCC
Restriction Sites NotI-NotI     
ACCN NM_001173
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001173.2, NP_001164.2
RefSeq Size 9601 bp
RefSeq ORF 4506 bp
Locus ID 394
UniProt ID Q13017
Domains RhoGAP, FF
Protein Pathways Focal adhesion, Leukocyte transendothelial migration
Gene Summary Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is one aa shorter compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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