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CDC20 (NM_001255) Human Untagged Clone
CAT＃: SC119337
CDC20 (untagged)-Human cell division cycle 20 homolog (S. cerevisiae) (CDC20)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bA276H19.3; CDC20A; p55CDC |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119337 sequence for NM_001255 edited (data generated by NextGen Sequencing)
ATGGCACAGTTCGCGTTCGAGAGTGACCTGCACTCGCTGCTTCAGCTGGATGCACCCATC CCCAATGCACCCCCTGCGCGCTGGCAGCGCAAAGCCAAGGAAGCCGCAGGCCCGGCCCCC TCACCCATGCGGGCCGCCAACCGATCCCACAGCGCCGGCAGGACTCCGGGCCGAACTCCT GGCAAATCCAGTTCCAAGGTTCAGACCACTCCTAGCAAACCTGGCGGTGACCGCTATATC CCCCATCGCAGTGCTGCCCAGATGGAGGTGGCCAGCTTCCTCCTGAGCAAGGAGAACCAG CCTGAAAACAGCCAGACGCCCACCAAGAAGGAACATCAGAAAGCCTGGGCTTTGAACCTG AACGGTTTTGATGTAGAGGAAGCCAAGATCCTTCGGCTCAGTGGAAAACCACAAAATGCG CCAGAGGGTTATCAGAACAGACTGAAAGTACTCTACAGCCAAAAGGCCACTCCTGGCTCC AGCCGGAAGACCTGCCGTTACATTCCTTCCCTGCCAGACCGTATCCTGGATGCGCCTGAA ATCCGAAATGACTATTACCTGAACCTTGTGGATTGGAGTTCTGGGAATGTACTGGCCGTG GCACTGGACAACAGTGTGTACCTGTGGAGTGCAAGCTCTGGTGACATCCTGCAGCTTTTG CAAATGGAGCAGCCTGGGGAATATATATCCTCTGTGGCCTGGATCAAAGAGGGCAACTAC TTGGCTGTGGGCACCAGCAGTGCTGAGGTGCAGCTATGGGATGTGCAGCAGCAGAAACGG CTTCGAAATATGACCAGTCACTCTGCCCGAGTGGGCTCCCTAAGCTGGAACAGCTATATC CTGTCCAGTGGTTCACGTTCTGGCCACATCCACCACCATGATGTTCGGGTAGCAGAACAC CATGTGGCCACACTGAGTGGCCACAGCCAGGAAGTGTGTGGGCTGCGCTGGGCCCCAGAT GGACGACATTTGGCCAGTGGTGGTAATGATAACTTGGTCAATGTGTGGCCTAGTGCTCCT GGAGAGGGTGGCTGGGTTCCTCTGCAGACATTCACCCAGCATCAAGGGGCTGTCAAGGCC GTAGCATGGTGTCCCTGGCAGTCCAATGTCCTGGCAACAGGAGGGGGCACCAGTGATCGA CACATTCGCATCTGGAATGTGTGCTCTGGGGCCTGTCTGAGTGCCGTGGATGCCCATTCC CAGGTGTGCTCCATCCTCTGGTCTCCCCATTACAAGGAGCTCATCTCAGGCCATGGCTTT GCACAGAACCAGCTAGTTATTTGGAAGTACCCAACCATGGCCAAGGTGGCTGAACTCAAA GGTCACACATCCCGGGTCCTGAGTCTGACCATGAGCCCAGATGGGGCCACAGTGGCATCC GCAGCAGCAGATGAGACCCTGAGGCTATGGCGCTGTTTTGAGTTGGACCCTGCGCGGCGG CGGGAGCGGGAGAAGGCCAGTGCAGCCAAAAGCAGCCTCATCCACCAAGGCATCCGCTGA Clone variation with respect to NM_001255.2 >OriGene 5' read for NM_001255 unedited
NGTCAACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGATTTTG TGGCCGGCCAGAGCGAAGGGTCCCTTTCTGTCCCCTGAGCACCGTCGCCTCCTTTCCTCC GGGCTCCGTAGGCACCAACTGCAAGGACCCCTCCCCCTGCGGGCGCTCCCATGGCACAGT TCGCGTTCGAGAGTGACCTGCACTCGCTGCTTCAGCTGGATGCACCCATCCCCAATGCAC CCCCTGCGCGCTGGCAGCGCAAAGCCAAGGAAGCCGCAGGCCCGGCCCCCTCACCCATGC GGGCCGCCAACCGATCCCACAGCGCCGGCAGGACTCCGGGCCGAACTCCTGGCAAATCCA GTTCCAAGGTTCAGACCACTCCTAGCAAACCTGGCGGTGACCGCTATATCCCCCATCGCA GTGCTGCCCAGATGGAGGTGGCCAGCTTCCTCCTGAGCAAGGAGAACCAGCCTGAAAACA GCCAGACGCCCACCAAGAAGGAACATCAGAAAGCCTGGGCTTTGAACCTGAACGGTTTTG ATGTAGAGGAAGCCAAGATCCTTCGGCTCAGTGGAAAACCACAAAATGCGCCAGAGGGTT ATCAGAACAGACTGAAAGTACTCTACAGCCAAAAGGCCACTCCTGGCTCCAGCCGGAAGA CCTGCCGTTACATTCCTTCCCTGCCAGACCGTATCCTGGATGCGCCTGAAATCCGAAATG ACTATTACCTGAACCTTGTGGNATTGGAGTTCNTGGGAATGTACTGGGCCGTGGCACTGG ACAACAGTGTGTACCTGTGGAGTGCAAGCTCTGGTGACATCCTGCAGCTTTTGCAATGGA GCAGCCTGGGGATATATATNCTCTGTGGCCTGGATCAAAGAGGGNCACTACTTGCTGTGG CACCANNCAGTGCTGAGTGCACTATGGGAAGTG >OriGene 3' read for NM_001255 unedited
NTTTTGCTCTGGACCGGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTAAA AAAAAAACATGAAGGGAGACATGACTTTATTAGAAAAAAAAAAACAACTGAGGTGATGGG TTGGTCTTCAGCGGATGCCTTGGTGGATGAGGCTGCTTTTGGCTGCACTGGCCTTCTCCC GCTCCCGCCGCCGCGCAGGGTCCAACTCAAAACAGCGCCATAGCCTCAGGGTCTCATCTG CTGCTGCGGATGCCACTGTGGCCCCATCTGGGCTCATGGTCAGACTCAGGACCCGGGATG TGTGACCTTTGAGTTCAGCCACCTTGGCCATGGTTGGGTACTTCCAAATAACTAGCTGGT TCTGTGCAAAGCCATGGCCTGAGATGAGCTCCTTGTAATGGGGAGACCAGAGGATGGAGC ACACCTGGGAATGGGCATCCACGGCACTCAGACAGGCCCCAGAGCACACATTCCAGATGC GAATGTGTCGATCACTGGTGCCCCCTCCTGTTGCCAGGACATTGGACTGCCAGGGACACC ATGCTACGGCCTTGACAGCCCCTTGATGCTGGGTGAATGTCTGCAGAGGAACCCAGCCAC CCTCTCCAGGAGCACTAGGCCACACATTGACCAAGTTATCATTACCACCACTGGCCAAAT GTCGTCCATCTGGGGCCCAGCGCAGCCCACACACTTNCTGGNCTGTGNGCCACTCAGTGT GGCCACATGGNTGTTCTGCTACCCGACATCATGGTGGNTGGGATGTGGCCCAGACGTGAA CCACTGGACAGATATTAGCTGNTCCAGCTTAGGNAGCCCCACTCGNCAGAGTGACTGGTN CATATTCNNAAGCCCGTTCTGCTGCTGCACATCCCATAGCTGCACCTAGCACTTGCTGGT GCCACAGNNCNNAGTAGTGCCTTCTTGGATCCAGCACAGAGGTATATATATTCCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001255 |
| Insert Size | 960 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001255.1, NP_001246.1 |
| RefSeq Size | 1686 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 991 |
| UniProt ID | Q12834 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Ubiquitin mediated proteolysis |
| Gene Summary | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200911 | CDC20 (Myc-DDK-tagged)-Human cell division cycle 20 homolog (S. cerevisiae) (CDC20) |
CNY 3,656.00 |
|
| RC200911L1 | Lenti ORF clone of Human cell division cycle 20 homolog (S. cerevisiae) (CDC20), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200911L2 | Lenti ORF clone of Human cell division cycle 20 homolog (S. cerevisiae) (CDC20), mGFP tagged |
CNY 6,056.00 |
|
| RC200911L3 | Lenti ORF clone of Human cell division cycle 20 homolog (S. cerevisiae) (CDC20), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200911L4 | Lenti ORF clone of Human cell division cycle 20 homolog (S. cerevisiae) (CDC20), mGFP tagged |
CNY 6,056.00 |
|
| RG200911 | CDC20 (tGFP-tagged) - Human cell division cycle 20 homolog (S. cerevisiae) (CDC20) |
CNY 5,256.00 |
|
| SC320629 | CDC20 (untagged)-Human cell division cycle 20 homolog (S. cerevisiae) (CDC20) |
CNY 5,488.00 |
