United States
Germany
Japan
United Kingdom
China
DGKA (NM_001345) Human Untagged Clone
CAT＃: SC119317
DGKA (untagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3
CNY 5,392.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAGK; DAGK1; DGK-alpha |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119317 sequence for NM_001345 edited (data generated by NextGen Sequencing)
ATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATACATG GAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGATGGCT AAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAATCTAT CTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGAGACT GGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGTGGTGTGTCTCAATGATGTTTCC TGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCTTCAAG CTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTATCCTA CAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGATTCTT CAGGAGATGATGAAAGAGATTGACTATGATGGCAGTGGCTCTGTCTCTCAAGCTGAGTGG GTCCGGGCTGGGGCCACCACCGTGCCACTGCTAGTGCTGCTGGGTCTGGAGATGACTCTG AAGGACGACGGACAGCACATGTGGAGGCCCAAGAGGTTCCCCAGACCAGTCTACTGCAAT CTGTGCGAGTCAAGCATTGGTCTTGGCAAACAGGGACTGAGCTGTAACCTCTGTAAGTAC ACTGTTCACGACCAGTGTGCCATGAAAGCCCTGCCTTGTGAAGTCAGCACCTATGCCAAG TCTCGGAAGGACATTGGTGTCCAATCACATGTGTGGGTGCGAGGAGGCTGTGAGTCCGGG CGCTGCGACCGCTGTCAGAAAAAGATCCGGATCTACCACAGTCTGACCGGGCTGCATTGT GTATGGTGCCACCTAGAGATCCACGATGACTGCCTGCAAGCGGTGGGCCATGAGTGTGAC NNNNNNCTGCTCCGGGATCACATCCTGCCTCCATCTTCCATCTATCCCAGTGTCCTGGCC TCTGGACCGGATCGTAAAAATAGCAAAACAAGCCAGAAGACCATGGATGATTTAAATTTG AGCACCTCTGAGGCTCTGCGGATTGACCCTGTTCCTAACACCCACCCACTTCTCGTCTTT GTCAATCCTAAGAGTGGCGGGAAGCAGGGNNNNAGGGTGCTCTGGAAGTTCCAGTATATA TTAAACCCTCGACAGGTGTTCAACCTCCTAAAGGATGGTCCTGAGATAGGGCTCCGATTA TTCAAGGATGTTCCTGATAGCCGGATTTTGGTGTGTGGTGGAGACGGCACAGTAGGCTGG ATTCTAGAGACCATTGACAAAGCTAACTTGCCAGTTTTGCCTCCTGTTGCTGTGTTGCCC CTGGGTACTGGAAATGATCTGGCTCGATGCCTAAGATGGGGAGGAGGTTATGAAGGACAG AATCTGGCAAAGATCCTCAAGGATTTAGAGATGAGTAAAGTGGTACATATGGATCGATGG TCTGTGGAGGTGATACCTCAACAAACTGAAGAAAAAAGTGACCCAGTCCCCTTTCAAATC ATCAATAACTACTTCTCTATTGGCGTGGATGCCTCTATTGCTCATCGATTCCACATCATG CGAGAGAAATATCCGGAGAAGTTCAACAGCAGAATGAAGAACAAGCTATGGTACTTCGAA TTTGCCACATCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAGGAGTCTTTGACAGTT GAGATCTGTGGGAAACCGCTGGATCTGAGCAACCTGTCCCTAGAAGGCATCGCAGTGCTA AACATCCCTAGCATGCATGGTGGCTCCAACCTCTGGGGTGATACCAGGAGACCCCATGGG GATATCTATGGGATCAACCAGGCCTTAGGTGCTACAGCTAAAGTCATCACCGACCCTGAT ATCCTGAAAACCTGTGTACCAGACCTAAGTGACAAGAGACTGGAAGTGGTTGGGCTGGAG GGTGCAATTGAGATGGGCCAAATCTATACCAAGCTCAAGAATGCTGGACGTCGGCTGGCC AAGTGCTCTGAGATCACCTTCCACACCACAAAAACCCTTCCCATGCAAATTGACGGAGAA CCCTGGATGCAGACGCCCTGTACAATCAAGATCACCCACAAGAACCAGATGCCCATGCTC ATGGGCCCACCCCCCCGCTCCACCAATTTCTTTGGCTTCTTGAGCTAA Clone variation with respect to NM_001345.4 961 t=>n;962 g=>n;963 t=>n;964 g=>n;965 g=>n;966 g=>n;1170 g=>n;1171 c=>n;1172 a=>n;1173 a=>n >OriGene 5' read for NM_001345 unedited
GTAATTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGAGTGAGTCCC TAGGCCTCCATCTCTCTCCCTTGCTGTACCACCTTCACCACCATCCATGCGACCCCAAGA GCCTTAATGACTCTAGAAGAGACTCCAGGCAGGGGAAGCTGAAAGGACCTTTCACTCCCT ACTTTTGGCCAGGGCCTTCTGTGCCACCTGCCAAGACCAGCAGGCCTACCCTCTGAAGAG GTCCAAGCAACGGAAGTACTACTACGAAGCTGCCTTTCTGGCCATCCTTGAGAAAAATAG ACAGATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATA CATGGAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGAT GGCTAAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAAT CTATCTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGA GACTGGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGGTGGTGTGTCTCAATGATG TTTCCTGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCT TCAAGCTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTA TCCTACAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGA TTCTTCAGGAGATGATGAAGAGATGCTATGATGGCAGGGGCTCTGTTCTCAACTGGAGGG GTCCGGGCTGGGGCCACACCGTGCCCTGCTATGCTT >OriGene 3' read for NM_001345 unedited
GGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGATCCCAAGGGGGAAACA CAAGTCATTTTATTCAGATGAGGCACTTTCAATGTATGTGTTTTGGGGTGTGTGTATGTG TGTGCGCATGGGGAACAGTGAGGTGGTGCAGGATAATAAATACTGTGAGGGTGAAGGAAC ACTCCCCCAAGCTGGCAGGAGTATGTGGCAGCAATGTACAGAGCCTCGGGAGTAGAGTCC AGGGACAGGGAGGTGGGTTCAAGGCTGGCTTGGAGGCCAAGGGTGTCCCCCTTAGCTCAA GAAGCCAAAAAAATTGGTGGAGCGGGGGGGTGGGCCCATGAGCATGGGCATCTGGTTCTT GTGGGTGATCTTGATTGTACAGGGCGTCTGCATCCAGGGTTCTCCGTCAATTTGCATGGG AAGGGTTTTTGTGGTGTGGAAGGTGATCTCAGAGCACTTGGCCAGCCGACGTCCAGCATT CTTGAGCTTGGTATAGATTTGGCCCATCTCAATTGCACCCTCCAGCCCAACCACTTCCAG TCTTTTGTTACTTACGTCTGGTACACAGGTTTTCAGGATATCACGGTCGGTGATGACTTT AACTGAAGCACCTAACGGCTGGTTGATCCCATATATATCCCCCATGGCGCGCTCCTGGTA TCACCTCCTAAACGCCGGAGCCCCCATGCGATTGTTAGGGATGGTTACGACCTGCGAACG CCTCTCCAGGGACAGGGTGCCCAAACCCCCCGGTTTCCCCACACATCTCCCTCCCCCAAC ACTTCCTTCGTTCTTTTATATGTCGGAAACGAACCCTGTTACCATCCCCAAACANGCGAA GACATANATAGTGTTCTTTATTTCCTTCCTCTATCTTTGTGGGAAATCGGTGTGGAATTG GGGGAGAGAGGGGCGCCCCGGCCGCGCCCTCCCATGACATTTCACTCCACCTTCTTCCGC CCCCCCCATTCGCCCTCCGTCTTCCCCTCCGCCCTCCCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001345 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001345.4, NP_001336.2 |
| RefSeq Size | 2756 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 1606 |
| UniProt ID | P23743 |
| Domains | DAGKa, DAGKc, EFh, DAG_PE-bind |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222395 | DGKA (Myc-DDK-tagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3 |
CNY 5,384.00 |
|
| RC222395L1 | Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, Myc-DDK-tagged |
CNY 7,784.00 |
|
| RC222395L2 | Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC222395L3 | Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222395L4 | Lenti ORF clone of Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3, mGFP tagged |
CNY 7,784.00 |
|
| RG222395 | DGKA (tGFP-tagged) - Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3 |
CNY 6,984.00 |
