CHML (NM_001821) Human Untagged Clone
CAT#: SC119005
CHML (untagged)-Human choroideremia-like (Rab escort protein 2) (CHML)
CNY 7,336.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | REP2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC119005 sequence for NM_001821 edited (data generated by NextGen Sequencing)
ATGGCGGACAATCTTCCCACAGAGTTTGATGTGGTTATAATAGGGACAGGTTTGCCCGAA TCCATCCTTGCAGCTGCATGTTCAAGAAGTGGTCAGAGGGTTCTGCATATTGATTCAAGA AGCTACTATGGAGGAAACTGGGCTAGTTTCAGCTTTTCAGGATTGCTATCCTGGTTGAAG GAGTATCAGCAAAACAATGACATTGGGGAAGAAAGTACTGTTGTATGGCAGGACCTGATC CATGAAACAGAAGAAGCCATCACTCTTCGCAAGAAGGATGAAACTATTCAACACACAGAA GCTTTTTGCTACGCCAGTCAGGATATGGAGGACAACGTTGAAGAGATTGGTGCTCTGCAG AAAAATCCTTCTTTGGGGGTGTCTAATACCTTCACTGAAGTTCTGGATTCTGCATTACCT GAAGAAAGCCAGTTATCGTATTTTAATAGCGACGAAATGCCTGCAAAACACACTCAGAAA AGTGATACAGAGATTTCACTAGAAGTAACTGATGTAGAGGAATCAGTGGAGAAGGAAAAG TATTGTGGAGATAAAACTTGTATGCACACAGTTTCAGATAAAGATGGAGATAAAGATGAA AGCAAATCTACAGTAGAAGATAAGGCCGATGAACCAATTAGAAATAGGATTACTTACTCT CAAATAGTTAAAGAAGGCAGGAGGTTTAATATTGATTTGGTGTCAAAACTGCTGTATTCT CAAGGATTGCTAATTGATCTTTTAATCAAATCAGATGTTAGTCGTTATGTAGAATTTAAA AATGTCACTAGGATTCTTGCATTTCGGGAAGGAAAGGTAGAACAAGTTCCTTGTTCCAGA GCAGATGTCTTTAATAGCAAGGAACTCACCATGGTTGAAAAGAGGATGCTAATGAAATTT CTCACATTTTGTTTAGAGTATGAACAACATCCTGATGAATACCAAGCTTTCAGGCAGTGT TCATTTTCAGAATACTTAAAAACTAAAAAACTAACTCCCAACCTTCAACATTTTGTACTG CACTCAATTGCAATGACATCAGAATCATCTTGCACTACAATAGATGGTCTTAACGCAACT AAAAACTTCCTTCAGTGTCTCGGACGGTTTGGCAACACCCCCTTTTTATTTCCCTTGTAT GGCCAAGGAGAAATTCCCCAGGGTTTCTGTAGGATGTGTGCAGTTTTTGGTGGAATCTAT TGTCTTCGTCATAAAGTACAATGCTTTGTAGTCGACAAAGAATCTGGACGATGTAAAGCA ATTATAGATCACTTTGGTCAAAGAATAAATGCTAAATATTTTATTGTGGAAGACAGTTAC CTTTCTGAGGAAACATGCTCAAATGTGCAGTATAAGCAGATCTCTAGGGCAGTACTCATT ACAGATCAGTCTATACTAAAGACAGATTTAGATCAGCAGACTTCCATTCTGATAGTTCCT CCAGCAGAGCCAGGAGCTTGTGCTGTACGGGTCACAGAATTATGTTCTTCAACCATGACA TGCATGAAGGACACCTATCTGGTACATTTGACATGTTCATCTTCTAAAACAGCAAGAGAA GACTTAGAATCAGTGGTGAAGAAATTATTCACTCCGTATACTGAAACAGAAATAAACGAG GAAGAACTTACAAAGCCAAGACTCTTGTGGGCTCTTTATTTTAATATGAGAGATTCCTCG GGAATCAGCAGAAGCTCGTATAATGGCTTGCCTTCCAATGTTTATGTCTGCTCTGGGCCT GACTGTGGCCTGGGAAATGAGCATGCTGTCAAGCAAGCTGAAACACTTTTCCAGGAGATC TTTCCAACTGAAGAATTCTGCCCTCCACCTCCAAATCCAGAAGACATTATCTTTGATGGT GATGATAAGCAGCCAGAGGCTCCTGGAACCAATAATGTAGTAATGGCCAAACTAGAATCC TCTGAGGAAAGCAAAAACCTAGAAAGCCCAGAGAAGCACCTTCAAAATTAG Clone variation with respect to NM_001821.3 >OriGene 5' read for NM_001821 unedited
AGCATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGGCGCTGCTG CTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTGCTGGTGCTCGTCCTCTACTAC AAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTGGTCAACATCAGCCTCAGCGAC CTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTGTCCTGCCTGAGGAACGGCTGG GTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGCGGCAGCCTCTTCGCAGTTGTC ACCAGACGCTGTTACTTCCTAAAATGGCGGACAATCTTCCCACAGAGTTTGATGTGGTTA TAATAGGGACAGGTTTGCCCGAATCCATCCTTGCAGCTGCATGTTCAAGAAGTGGTCAGA GGGTTCTGCATATTGATTCAAGAAGCTACTATGGAGGAAACTGGGCTAGTTTCAGCTTTT CAGGATTGCTATCCTGGTTGAAGGAGTATCAGCAAAACAATGACATTGGGGAAGAAAGTA CTGTTGTATGGCAGGACCTGATCCATGAAACAGAAGAAGCCATCACTCTTCGCAAGAAGG ATGAAACTATTCAACACACAGAAGCTTTTTGCTACGCCAGTCAGGATATGGAGGACAACG TTGAAGAGATTGGTGCTCTGCAGAAAAATCCTTCTTTGGGGGTGTCTAATACCTTCACTG AAGTTCTGGATTCTGCATTACCTGAAGAAAGCCAGTTATCGTATTTTAATAGCGACGAAA TGCCTGCANAACACACTCAGAAAAGTGATACAGNNAGATTCACTAGAGTAACTGATGTAG AGGAATCAGTGGAGAAGGGGAAAGTATGTGGAGATAAAAACTGTATGCACACAGTNTCAG ATAAGATGGAGATAAGATGAAGCAATCTACGTAGAAGATAGGCCGATGAACCATAGAAAT AGATACTACTCTCAT >OriGene 3' read for NM_001821 unedited
TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAACGATTATAGACAAATGTATTTTATTAT CATATTGGCATTTGTATTAGTTGCTATTCCTACCACCATGTCTATACATTTATATTATAT GCAAAAGATCCACATAATTTACCATTTGTGTATGTTTCACTATTCGAACAGGCCGCAGCA ACTGTAACAGCTGCAAAAGTAATTAAAATATTACTTGGGTATTACTAATTGTTCTGTAGA CAAAGATAAAATCTGAAGATCATTAGCAAATACATTACCTTTAAGCTGAGTTGCTAATAT GGAACCCTTACATATATACCCAATACTAAAATAAAGCTAACTCTGTTCTAAGATTCTGGG TCATATAGCCCATTCTAAACAAAACGTTCAATACTCAATAAATCACTCATTTGATAGGTT AACAAAGATATTCAATGCCTGAATGTGTGAGAGTTAAAGACACCATTCTGCATAGCATCC ATCATATATAACCCACTTCTAATTTACTGATATGGGAAAACCGTCCTTTAAAATGAGAAA TCGTGATGCCATGGAAGAGGTCCAAACAGTATCTGCAGATTGCTCTTTGTTATTTTGAAG GTGCTTCTCTGGATTTACTAGCATTTTGTTTATCTCGAAGGATTCAACTTGGACATTACT ACATTTATGGCTCCCAGCACCCTCTGCCGGTTATCTTCCCATACAACATATGCCCTCGGA TTTGCGAGCGGAGGGAAAACATTTAACTGCAAAGACTTCTGCAAATGGCATAATTTGTTG CCATCGCCCTTTTTCAGCCCCCTCCGCCTTACCTTATACCCTTGAAGGGCACTCTTTCAC CTTTGCGGTCCCCGGGATTTCTTCCTACAATACCCCCAAATCCGGTCTGAAAAATCCCGA TTTTTCGCCGCATCGGCGGAATCTCCTCCCCCGCCTAACATTCTTTGTTTTAACAAAATC CCTGCCCCACGTGCTCTTC |
Restriction Sites | NotI-NotI |
ACCN | NM_001821 |
Insert Size | 2820 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001821.2, NP_001812.2 |
RefSeq Size | 7070 bp |
RefSeq ORF | 1971 bp |
Locus ID | 1122 |
UniProt ID | P26374 |
Domains | GDI |
Gene Summary | The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008] |
Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218675 | CHML (Myc-DDK-tagged)-Human choroideremia-like (Rab escort protein 2) (CHML) |
CNY 7,328.00 |
|
RC218675L3 | Lenti-ORF clone of CHML (Myc-DDK-tagged)-Human choroideremia-like (Rab escort protein 2) (CHML) |
CNY 6,460.00 |
|
RC218675L4 | Lenti-ORF clone of CHML (mGFP-tagged)-Human choroideremia-like (Rab escort protein 2) (CHML) |
CNY 6,460.00 |
|
RG218675 | CHML (tGFP-tagged) - Human choroideremia-like (Rab escort protein 2) (CHML) |
CNY 8,928.00 |