CHML (NM_001821) Human Untagged Clone

CAT#: SC119005

CHML (untagged)-Human choroideremia-like (Rab escort protein 2) (CHML)



  "NM_001821" in other vectors (4)

CNY 7,336.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit polyclonal anti-CHML antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms REP2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119005 sequence for NM_001821 edited (data generated by NextGen Sequencing)
ATGGCGGACAATCTTCCCACAGAGTTTGATGTGGTTATAATAGGGACAGGTTTGCCCGAA
TCCATCCTTGCAGCTGCATGTTCAAGAAGTGGTCAGAGGGTTCTGCATATTGATTCAAGA
AGCTACTATGGAGGAAACTGGGCTAGTTTCAGCTTTTCAGGATTGCTATCCTGGTTGAAG
GAGTATCAGCAAAACAATGACATTGGGGAAGAAAGTACTGTTGTATGGCAGGACCTGATC
CATGAAACAGAAGAAGCCATCACTCTTCGCAAGAAGGATGAAACTATTCAACACACAGAA
GCTTTTTGCTACGCCAGTCAGGATATGGAGGACAACGTTGAAGAGATTGGTGCTCTGCAG
AAAAATCCTTCTTTGGGGGTGTCTAATACCTTCACTGAAGTTCTGGATTCTGCATTACCT
GAAGAAAGCCAGTTATCGTATTTTAATAGCGACGAAATGCCTGCAAAACACACTCAGAAA
AGTGATACAGAGATTTCACTAGAAGTAACTGATGTAGAGGAATCAGTGGAGAAGGAAAAG
TATTGTGGAGATAAAACTTGTATGCACACAGTTTCAGATAAAGATGGAGATAAAGATGAA
AGCAAATCTACAGTAGAAGATAAGGCCGATGAACCAATTAGAAATAGGATTACTTACTCT
CAAATAGTTAAAGAAGGCAGGAGGTTTAATATTGATTTGGTGTCAAAACTGCTGTATTCT
CAAGGATTGCTAATTGATCTTTTAATCAAATCAGATGTTAGTCGTTATGTAGAATTTAAA
AATGTCACTAGGATTCTTGCATTTCGGGAAGGAAAGGTAGAACAAGTTCCTTGTTCCAGA
GCAGATGTCTTTAATAGCAAGGAACTCACCATGGTTGAAAAGAGGATGCTAATGAAATTT
CTCACATTTTGTTTAGAGTATGAACAACATCCTGATGAATACCAAGCTTTCAGGCAGTGT
TCATTTTCAGAATACTTAAAAACTAAAAAACTAACTCCCAACCTTCAACATTTTGTACTG
CACTCAATTGCAATGACATCAGAATCATCTTGCACTACAATAGATGGTCTTAACGCAACT
AAAAACTTCCTTCAGTGTCTCGGACGGTTTGGCAACACCCCCTTTTTATTTCCCTTGTAT
GGCCAAGGAGAAATTCCCCAGGGTTTCTGTAGGATGTGTGCAGTTTTTGGTGGAATCTAT
TGTCTTCGTCATAAAGTACAATGCTTTGTAGTCGACAAAGAATCTGGACGATGTAAAGCA
ATTATAGATCACTTTGGTCAAAGAATAAATGCTAAATATTTTATTGTGGAAGACAGTTAC
CTTTCTGAGGAAACATGCTCAAATGTGCAGTATAAGCAGATCTCTAGGGCAGTACTCATT
ACAGATCAGTCTATACTAAAGACAGATTTAGATCAGCAGACTTCCATTCTGATAGTTCCT
CCAGCAGAGCCAGGAGCTTGTGCTGTACGGGTCACAGAATTATGTTCTTCAACCATGACA
TGCATGAAGGACACCTATCTGGTACATTTGACATGTTCATCTTCTAAAACAGCAAGAGAA
GACTTAGAATCAGTGGTGAAGAAATTATTCACTCCGTATACTGAAACAGAAATAAACGAG
GAAGAACTTACAAAGCCAAGACTCTTGTGGGCTCTTTATTTTAATATGAGAGATTCCTCG
GGAATCAGCAGAAGCTCGTATAATGGCTTGCCTTCCAATGTTTATGTCTGCTCTGGGCCT
GACTGTGGCCTGGGAAATGAGCATGCTGTCAAGCAAGCTGAAACACTTTTCCAGGAGATC
TTTCCAACTGAAGAATTCTGCCCTCCACCTCCAAATCCAGAAGACATTATCTTTGATGGT
GATGATAAGCAGCCAGAGGCTCCTGGAACCAATAATGTAGTAATGGCCAAACTAGAATCC
TCTGAGGAAAGCAAAAACCTAGAAAGCCCAGAGAAGCACCTTCAAAATTAG

Clone variation with respect to NM_001821.3
>OriGene 5' read for NM_001821 unedited
AGCATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGGCGCTGCTG
CTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTGCTGGTGCTCGTCCTCTACTAC
AAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTGGTCAACATCAGCCTCAGCGAC
CTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTGTCCTGCCTGAGGAACGGCTGG
GTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGCGGCAGCCTCTTCGCAGTTGTC
ACCAGACGCTGTTACTTCCTAAAATGGCGGACAATCTTCCCACAGAGTTTGATGTGGTTA
TAATAGGGACAGGTTTGCCCGAATCCATCCTTGCAGCTGCATGTTCAAGAAGTGGTCAGA
GGGTTCTGCATATTGATTCAAGAAGCTACTATGGAGGAAACTGGGCTAGTTTCAGCTTTT
CAGGATTGCTATCCTGGTTGAAGGAGTATCAGCAAAACAATGACATTGGGGAAGAAAGTA
CTGTTGTATGGCAGGACCTGATCCATGAAACAGAAGAAGCCATCACTCTTCGCAAGAAGG
ATGAAACTATTCAACACACAGAAGCTTTTTGCTACGCCAGTCAGGATATGGAGGACAACG
TTGAAGAGATTGGTGCTCTGCAGAAAAATCCTTCTTTGGGGGTGTCTAATACCTTCACTG
AAGTTCTGGATTCTGCATTACCTGAAGAAAGCCAGTTATCGTATTTTAATAGCGACGAAA
TGCCTGCANAACACACTCAGAAAAGTGATACAGNNAGATTCACTAGAGTAACTGATGTAG
AGGAATCAGTGGAGAAGGGGAAAGTATGTGGAGATAAAAACTGTATGCACACAGTNTCAG
ATAAGATGGAGATAAGATGAAGCAATCTACGTAGAAGATAGGCCGATGAACCATAGAAAT
AGATACTACTCTCAT
>OriGene 3' read for NM_001821 unedited
TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAACGATTATAGACAAATGTATTTTATTAT
CATATTGGCATTTGTATTAGTTGCTATTCCTACCACCATGTCTATACATTTATATTATAT
GCAAAAGATCCACATAATTTACCATTTGTGTATGTTTCACTATTCGAACAGGCCGCAGCA
ACTGTAACAGCTGCAAAAGTAATTAAAATATTACTTGGGTATTACTAATTGTTCTGTAGA
CAAAGATAAAATCTGAAGATCATTAGCAAATACATTACCTTTAAGCTGAGTTGCTAATAT
GGAACCCTTACATATATACCCAATACTAAAATAAAGCTAACTCTGTTCTAAGATTCTGGG
TCATATAGCCCATTCTAAACAAAACGTTCAATACTCAATAAATCACTCATTTGATAGGTT
AACAAAGATATTCAATGCCTGAATGTGTGAGAGTTAAAGACACCATTCTGCATAGCATCC
ATCATATATAACCCACTTCTAATTTACTGATATGGGAAAACCGTCCTTTAAAATGAGAAA
TCGTGATGCCATGGAAGAGGTCCAAACAGTATCTGCAGATTGCTCTTTGTTATTTTGAAG
GTGCTTCTCTGGATTTACTAGCATTTTGTTTATCTCGAAGGATTCAACTTGGACATTACT
ACATTTATGGCTCCCAGCACCCTCTGCCGGTTATCTTCCCATACAACATATGCCCTCGGA
TTTGCGAGCGGAGGGAAAACATTTAACTGCAAAGACTTCTGCAAATGGCATAATTTGTTG
CCATCGCCCTTTTTCAGCCCCCTCCGCCTTACCTTATACCCTTGAAGGGCACTCTTTCAC
CTTTGCGGTCCCCGGGATTTCTTCCTACAATACCCCCAAATCCGGTCTGAAAAATCCCGA
TTTTTCGCCGCATCGGCGGAATCTCCTCCCCCGCCTAACATTCTTTGTTTTAACAAAATC
CCTGCCCCACGTGCTCTTC
Restriction Sites NotI-NotI     
ACCN NM_001821
Insert Size 2820 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001821.2, NP_001812.2
RefSeq Size 7070 bp
RefSeq ORF 1971 bp
Locus ID 1122
UniProt ID P26374
Domains GDI
Gene Summary The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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