EIF5A (NM_001970) Human Untagged Clone

CAT#: SC118910

EIF5A (untagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant B



  "NM_001970" in other vectors (6)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit anti-EIF5A Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms eIF-4D; EIF-5A; EIF5A1; eIF5AI
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118910 sequence for NM_001970 edited (data generated by NextGen Sequencing)
ATGGCAGATGACTTGGACTTCGAGACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATG
CAGTGCTCAGCATTACGTAAGAATGGCTTTGTGGTGCTCAAAGGCCGGCCATGTAAGATC
GTCGAGATGTCTACTTCGAAGACTGGCAAGCACGGCCACGCCAAGGTCCATCTGGTTGGT
ATTGACATCTTTACTGGGAAGAAATATGAAGATATCTGCCCGTCAACTCATAATATGGAT
GTCCCCAACATCAAAAGGAATGACTTCCAGCTGATTGGCATCCAGGATGGGTACCTATCA
CTGCTCCAGGACAGCGGGGAGGTACGAGAGGACCTTCGTCTCCCTGAGGGAGACCTTGGC
AAGGAGATTGAGCAGAAGTACGACTGTGGAGAAGAGATCCTGATCACGGTGCTGTCTGCC
ATGACAGAGGAGGCAGCTGTTGCAATCAAGGCCATGGCAAAATAA

Clone variation with respect to NM_001970.4
>OriGene 5' read for NM_001970 unedited
GGTGTACGAATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCGGTA
GAGGCGGCGGCGGCGGCGGCAGCGGGCTCGGAGGCAGCGGTTGGGCTCGCGGCGAGCGGA
CGGGGTCGAGTCAGTGCGTTCGCGCGAGTTGGAATCGAAGCCTCTTAAAATGGCAGATGA
CTTGGACTTCGAGACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATGCAGTGCTCAGC
ATTACGTAAGAATGGCTTTGTGGTGCTCAAAGGCCGGCCATGTAAGATCGTCGAGATGTC
TACTTCGAAGACTGGCAAGCACGGCCACGCCAAGGTCCATCTGGTTGGTATTGACATCTT
TACTGGGAAGAAATATGAAGATATCTGCCCGTCAACTCATAATATGGATGTCCCCAACAT
CAAAAGGAATGACTTCCAGCTGATTGGCATCCAGGATGGGTACCTATCACTGCTCCAGGA
CAGCGGGGAGGTACGAGAGGACCTTCGTCTCCCTGAGGGAGACCTTGGCAAGGAGATTGA
GCAGAAGTACGACTGTGGAGAAGAGATCCTGATCACGGTGCTGTCTGCCATGACAGAGGA
GGCAGCTGTTGCAATCAAGGCCATGGCAAAATAACTGGCTCCCAGGGTGGCGGTGGTGGC
AGCAGTGATCCTCTGAACCTGCAGAGGCCCCCTCCCCGAGCCTGGCCTGGCTCTGGCCCG
GTCCTAAGCTGGACTCCTCCTACACAATTTATTTGACGTTNTATTTTGGTTTTCCCCACC
CCCTCATCTGTCGNGGAGCCCCTGCCCTCCACCTAGCTCCTTGGCCAGNAGCGAGCGAAG
CTGTGCCCTTGGTGAAGCTGCCCTCCTCTTCTCCCTNACACTAAGCCCTGGTGGGAAAGG
GGGGNGGGGGCNCCCCCCNC
>OriGene 3' read for NM_001970 unedited
AGCTTGTNGTTCTATTCATGNTTGATTTTAACAAATGGTGTCGGGGNANANAGCCCGCAG
GGAAGGGTAAAGCCCATGGGGGCAGGGCCCTCCCAGATGCCTGAGGAGGGGGCAGGTCCC
CTCCCCTCTCCTCCTCTTCCCTCCCCATCTAAAGGGGTTTGGGGAGAGACACAGGCAGGC
GAGGGGGCTGGTCCCCAGTCTGTTGGGGTGGTGCTTATGGTAAAGGGCTATGGGCAACAG
GGGACCAGACCAGGGATGAGTGGGGAGGGCACAAGGACCATTTGCCAGAATCCACCGCTT
TCTGATTCCAGATTGAATTAAAAAAAAAAAAAAAAAAAAAAATTGACTAACCCACAAGCA
GCACCCACCCCCTTCTCCCCCACCAGGGCTGTACTGTGAGGGGAGAAGAGGAGGGCAGCT
TCACCAAGGCCACAGCTTCGCTCGCTTCTGGCCAAGGGAGCTANGTGAAGGGCCAGGGCT
CCCCCACAGATTGACGGGGTGGGGAAAACCAAACTCAACTTTCNATAAATTGTGNTNGAG
GAGTCCAGTTTATGACCGCGCCATACCTCAGCCACAGCTCGGGAAGGGGGCCTCTCCTGC
TTCAAACGGTCACTGNTGCCAACTCCACCACTCTAGCTCCAGTTTTTTTCGCTAGCCCTC
CCCGCACCCCTACCCTTTCCGCAGTGGAACACCTATATTTAACCTGTCCCTTCTCAATCG
CCTCTCTAGACTCAATTGCTTTCCCAAGTTCCCCTCACGGTAATCAAGTTCCTCCCCCCC
TCTCTTTCTTTCCACAAAGTCCGTCCCATCTTCTCTTCCACCCCTTTTCATAATCNCCTC
CCCATGTCCCNACCCCCGACNTTCTCCACTACCCTCTTTCCCACATTTCGTTAATCTCCC
CTTTAACGCCCCTTCTTCTCCTCCAAACTTTTTCCCTACCNTAACATCTTGTATCCN
Restriction Sites NotI-NotI     
ACCN NM_001970
Insert Size 6680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001970.3, NP_001961.1
RefSeq Size 1290 bp
RefSeq ORF 465 bp
Locus ID 1984
UniProt ID P63241
Domains eIF-5a, KOW
Gene Summary mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (B) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon compared to variant A. The encoded isoform (B) has a shorter N-terminus compared to isoform A. Variants B, C, and D encode the same isoform.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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