EEF2 (NM_001961) Human Untagged Clone
CAT#: SC118904
EEF2 (untagged)-Human eukaryotic translation elongation factor 2 (EEF2)
CNY 9,432.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | EEF-2; EF-2; EF2; SCA26 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_001961 edited
CGCTTCTTTCGGTTCTACCTGGGAGAATCCACCGCCATCCGCCACCATGGTGAACTTCAC GGTAGACCAGATCCGCGCCATCATGGACAAGAAGGCCAACATCCGCAACATGTCTGTCAT CGCCCACGTGGACCATGGCAAGTCCACGCTGACAGACTCCCTGGTGTGCAAGGCGGGCAT CATCGCCTCGGCCCGGGCCGGGGAGACACGCTTCACTGATACCCGGAAGGACGAGCAGGA GCGTTGCATCACCATCAAGTCAACTGCCATCTCCCTCTTCTACGAGCTCTCGGAGAATGA CTTGAACTTCATCAAGCAGAGCAAGGACGGTGCCGGCTTCCTCATCAACCTCATTGACTC CCCCGGGCATGTCGACTTCTCCTCGGAGGTGACTGCTGCCCTCCGAGTCACCGATGGCGC ATTGGTGGTGGTGGACTGCGTGTCAGGCGTGTGCGTGCAGACGGAGACAGTGCTGCGGCA GGCCATTGCCGAGCGCATCAAGCCTGTGCTGATGATGAACAAGATGGACCGCGCCCTGCT GGAGCTGCAGCTGGAGCCCGAGGAGCTCTACCAGACTTTCCAGCGCATCGTGGAGAACGT GAACGTCATCATCTCCACCTACGGCGAGGGCGAGAGCGGCCCCATGGGCAACATCATGAT CGATCCTGTCCTCGGTACCGTGGGCTTTGGGTCTGGCCTCCACGGGTGGGCCTTCACCCT GAAGCAGTTTGCCGAGATGTATGTGGCCAAGTTCGCCGCCAAGGGGGAGGGCCAGTTGGG GCCTGCCGAGCGGGCCAAGAAAGTAGAGGACATGATGAAGAAGCTGTGGGGTGACAGGTA CTTTGACCCAGCCAACGGCAAGTTCAGCAAGTCAGCCACCAGCCCCGAAGGGAAGAAGCT GCCACGCACCTTCTGCCAGCTGATCCTGGACCCCATCTTCAAGGTGTTTGATGCGATCAT GAATTTCAAGAAAGAGGAGACAGCAAAACTGATAGAGAAACTGGACATCAAACTGGACAG CGAGGACAAGGACAAAGAAGGCAAACCCCTGCTGAAGGCTGTGATGCGCCGCTGGCTGCC TGCCGGAGACGCCTTGTTGCAGATGATCACCATCCACCTGCCCTCCCCTGTGACGGCCCA GAAGTACCGCTGCGAGCTCCTGTACGAGGGGCCCCCGGACGACGAGGCTGCCATGGGCAT TAAAAGCTGTGACCCCAAAGGCCCTCTTATGATGTATATTTCCAAAATGGTGCCAACCTC CGACAAAGGTCGGTTCTACGCCTTTGGACGAGTCTTCTCGGGGCTGGTCTCCACTGGCCT GAAGGTCAGGATCATGGGGCCCAACTATACCCCTGGGAAGAAGGAGGACCTCTACCTGAA GCCAATCCAGAGAACAATCTTGATGATGGGCCGCTACGTGGAGCCCATCGAGGATGTGCC TTGTGGGAACATTGTGGGCCTCGTGGGCGTGGACCAGTTCCTGGTGAAGACGGGCACCAT CACCACCTTCGAGCACGCGCACAACATGCGGGTGATGAAGTTCAGCGTCAGCCCTGTTGT CAGAGTGGCCGTGGAGGCCAAGAACCCGGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAA GCGGCTGGCCAAGTCCGACCCCATGGTGCAGTGCATCATCGAGGAGTCGGGAGAGCATAT CATCGCGGGCGCCGGCGAGCTGCACCTGGAGATCTGCCTGAAGGACCTGGAGGAGGACCA CGCCTGCATCCCCATCAAGAAATCTGACCCGGTCGTCTCGTACCGCGAGACGGTCAGTGA AGAGTCGAACGTGCTCTGCCTCTCCAAGTCCCCCAACAAGCACAACCGGCTGTACATGAA GGCGCGGCCCTTCCCCGACGGCCTGGCCGAGGACATCGATAAAGGCGAGGTGTCCGCCCG TCAGGAGCTCAAGCAGCGGGCGCGCTACCTGGCCGAGAAGTACGAGTGGGACGTGGCTGA GGCCCGCAAGATCTGGTGCTTTGGGCCCGACGGCACCGGCCCCAACATCCTCACCGACAT CACCAAGGGTGTGCAGTACCTCAACGAGATCAAGGACAGTGTGGTGGCCGGCTTCCAGTG GGCCACCAAGGAGGGCGCACTGTGTGAGGAGAACATGCGGGGTGTGCGCTTCGACGTCCA CGACGTCACCCTGCACGCCGACGCCATCCACCGCGGAGGGGGCCAGATCATCCCCACAGC ACGGCGCTGCCTCTATGCCAGTGTGCTGACCGCCCAGCCACGCCTCATGGAGCCCATCTA CCTTGTGGAGATCCAGTGTCCAGAGCAGGTGGTCGGTGGCATCTACGGGGTTTTGAACAG GAAGCGGGGCCACGTGTTCGAGGAGTCCCAGGTGGCCGGCACCCCCATGTTTGTGGTCAA GGCCTATCTGCCCGTCAACGAGTCCTTTGGCTTCACCGCTGACCTGAGGTCCAACACGGG CGGCCAGGCGTTCCCCCAGTGTGTGTTTGACCACTGGCAGATCCTGCCCGGAGACCCCTT CGACAACAGCAGCTGCCCCAGCCAGGTGGTGGCGGAGACCCGCAAGCGCAAGGGCCTGAA AGAAGGCATCCCTGCCCTGGACAACTTCCTGGACAAATTGTAGGCGGCCCTTCCTGCAGC GCCTGCCGCCCCGGGGACTCGCAGCACCCACAGCACCACGTCCTCGAATTCTCAGACGAC ACCTGGAGACTGTCCCGACACAGCGACGCTCCCCTGAGAGGTTTCTGGGGCCCGCTGCGT GCCATCACTCAACCATAACACTTGATGCCGTTTCTTTCAATATTTATTTCCAGAGTCCGG AGGCAGCAGACACGCCCTCTTAGTAGGGACTTAATGGGCCGGTCGGGGAGGGGGAGGCGG GATGGGACACCCAACACTTTTTCCATTTCTTCAGAGGGAAACTCAGATGTCCAAACTAAT TTTAACAAACGCATTAAGAGGTTTATTTGGGTACATGGCCCGCAGTGGCTTTTGCCCCAG AAAGGGGAAAGGAACACGCGGGTAGATGATTTCTAGCAGGCAGGAAGTCCTGTGCGGTGT CACCATGAGCACCTCCAGCTGTACTAGTGCCATTGGAATAATAAATTTGATAAGGTGGTG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_001961 |
Insert Size | 3200 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001961.2. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001961.2, NP_001952.1 |
RefSeq Size | 3148 bp |
RefSeq ORF | 2577 bp |
Locus ID | 1938 |
UniProt ID | P13639 |
Domains | EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV |
Gene Summary | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212420 | EEF2 (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 2 (EEF2) |
CNY 9,424.00 |
|
RC212420L1 | Lenti ORF clone of Human eukaryotic translation elongation factor 2 (EEF2), Myc-DDK-tagged |
CNY 11,824.00 |
|
RC212420L2 | Lenti ORF clone of Human eukaryotic translation elongation factor 2 (EEF2), mGFP tagged |
CNY 9,030.00 |
|
RC212420L3 | Lenti ORF clone of Human eukaryotic translation elongation factor 2 (EEF2), Myc-DDK-tagged |
CNY 9,030.00 |
|
RC212420L4 | Lenti ORF clone of Human eukaryotic translation elongation factor 2 (EEF2), mGFP tagged |
CNY 11,824.00 |
|
RG212420 | EEF2 (tGFP-tagged) - Human eukaryotic translation elongation factor 2 (EEF2) |
CNY 11,024.00 |