EEF2 (NM_001961) Human Untagged Clone

CAT#: SC118904

EEF2 (untagged)-Human eukaryotic translation elongation factor 2 (EEF2)



  "NM_001961" in other vectors (6)

CNY 9,432.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-EEF2 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EEF-2; EF-2; EF2; SCA26
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001961 edited
CGCTTCTTTCGGTTCTACCTGGGAGAATCCACCGCCATCCGCCACCATGGTGAACTTCAC
GGTAGACCAGATCCGCGCCATCATGGACAAGAAGGCCAACATCCGCAACATGTCTGTCAT
CGCCCACGTGGACCATGGCAAGTCCACGCTGACAGACTCCCTGGTGTGCAAGGCGGGCAT
CATCGCCTCGGCCCGGGCCGGGGAGACACGCTTCACTGATACCCGGAAGGACGAGCAGGA
GCGTTGCATCACCATCAAGTCAACTGCCATCTCCCTCTTCTACGAGCTCTCGGAGAATGA
CTTGAACTTCATCAAGCAGAGCAAGGACGGTGCCGGCTTCCTCATCAACCTCATTGACTC
CCCCGGGCATGTCGACTTCTCCTCGGAGGTGACTGCTGCCCTCCGAGTCACCGATGGCGC
ATTGGTGGTGGTGGACTGCGTGTCAGGCGTGTGCGTGCAGACGGAGACAGTGCTGCGGCA
GGCCATTGCCGAGCGCATCAAGCCTGTGCTGATGATGAACAAGATGGACCGCGCCCTGCT
GGAGCTGCAGCTGGAGCCCGAGGAGCTCTACCAGACTTTCCAGCGCATCGTGGAGAACGT
GAACGTCATCATCTCCACCTACGGCGAGGGCGAGAGCGGCCCCATGGGCAACATCATGAT
CGATCCTGTCCTCGGTACCGTGGGCTTTGGGTCTGGCCTCCACGGGTGGGCCTTCACCCT
GAAGCAGTTTGCCGAGATGTATGTGGCCAAGTTCGCCGCCAAGGGGGAGGGCCAGTTGGG
GCCTGCCGAGCGGGCCAAGAAAGTAGAGGACATGATGAAGAAGCTGTGGGGTGACAGGTA
CTTTGACCCAGCCAACGGCAAGTTCAGCAAGTCAGCCACCAGCCCCGAAGGGAAGAAGCT
GCCACGCACCTTCTGCCAGCTGATCCTGGACCCCATCTTCAAGGTGTTTGATGCGATCAT
GAATTTCAAGAAAGAGGAGACAGCAAAACTGATAGAGAAACTGGACATCAAACTGGACAG
CGAGGACAAGGACAAAGAAGGCAAACCCCTGCTGAAGGCTGTGATGCGCCGCTGGCTGCC
TGCCGGAGACGCCTTGTTGCAGATGATCACCATCCACCTGCCCTCCCCTGTGACGGCCCA
GAAGTACCGCTGCGAGCTCCTGTACGAGGGGCCCCCGGACGACGAGGCTGCCATGGGCAT
TAAAAGCTGTGACCCCAAAGGCCCTCTTATGATGTATATTTCCAAAATGGTGCCAACCTC
CGACAAAGGTCGGTTCTACGCCTTTGGACGAGTCTTCTCGGGGCTGGTCTCCACTGGCCT
GAAGGTCAGGATCATGGGGCCCAACTATACCCCTGGGAAGAAGGAGGACCTCTACCTGAA
GCCAATCCAGAGAACAATCTTGATGATGGGCCGCTACGTGGAGCCCATCGAGGATGTGCC
TTGTGGGAACATTGTGGGCCTCGTGGGCGTGGACCAGTTCCTGGTGAAGACGGGCACCAT
CACCACCTTCGAGCACGCGCACAACATGCGGGTGATGAAGTTCAGCGTCAGCCCTGTTGT
CAGAGTGGCCGTGGAGGCCAAGAACCCGGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAA
GCGGCTGGCCAAGTCCGACCCCATGGTGCAGTGCATCATCGAGGAGTCGGGAGAGCATAT
CATCGCGGGCGCCGGCGAGCTGCACCTGGAGATCTGCCTGAAGGACCTGGAGGAGGACCA
CGCCTGCATCCCCATCAAGAAATCTGACCCGGTCGTCTCGTACCGCGAGACGGTCAGTGA
AGAGTCGAACGTGCTCTGCCTCTCCAAGTCCCCCAACAAGCACAACCGGCTGTACATGAA
GGCGCGGCCCTTCCCCGACGGCCTGGCCGAGGACATCGATAAAGGCGAGGTGTCCGCCCG
TCAGGAGCTCAAGCAGCGGGCGCGCTACCTGGCCGAGAAGTACGAGTGGGACGTGGCTGA
GGCCCGCAAGATCTGGTGCTTTGGGCCCGACGGCACCGGCCCCAACATCCTCACCGACAT
CACCAAGGGTGTGCAGTACCTCAACGAGATCAAGGACAGTGTGGTGGCCGGCTTCCAGTG
GGCCACCAAGGAGGGCGCACTGTGTGAGGAGAACATGCGGGGTGTGCGCTTCGACGTCCA
CGACGTCACCCTGCACGCCGACGCCATCCACCGCGGAGGGGGCCAGATCATCCCCACAGC
ACGGCGCTGCCTCTATGCCAGTGTGCTGACCGCCCAGCCACGCCTCATGGAGCCCATCTA
CCTTGTGGAGATCCAGTGTCCAGAGCAGGTGGTCGGTGGCATCTACGGGGTTTTGAACAG
GAAGCGGGGCCACGTGTTCGAGGAGTCCCAGGTGGCCGGCACCCCCATGTTTGTGGTCAA
GGCCTATCTGCCCGTCAACGAGTCCTTTGGCTTCACCGCTGACCTGAGGTCCAACACGGG
CGGCCAGGCGTTCCCCCAGTGTGTGTTTGACCACTGGCAGATCCTGCCCGGAGACCCCTT
CGACAACAGCAGCTGCCCCAGCCAGGTGGTGGCGGAGACCCGCAAGCGCAAGGGCCTGAA
AGAAGGCATCCCTGCCCTGGACAACTTCCTGGACAAATTGTAGGCGGCCCTTCCTGCAGC
GCCTGCCGCCCCGGGGACTCGCAGCACCCACAGCACCACGTCCTCGAATTCTCAGACGAC
ACCTGGAGACTGTCCCGACACAGCGACGCTCCCCTGAGAGGTTTCTGGGGCCCGCTGCGT
GCCATCACTCAACCATAACACTTGATGCCGTTTCTTTCAATATTTATTTCCAGAGTCCGG
AGGCAGCAGACACGCCCTCTTAGTAGGGACTTAATGGGCCGGTCGGGGAGGGGGAGGCGG
GATGGGACACCCAACACTTTTTCCATTTCTTCAGAGGGAAACTCAGATGTCCAAACTAAT
TTTAACAAACGCATTAAGAGGTTTATTTGGGTACATGGCCCGCAGTGGCTTTTGCCCCAG
AAAGGGGAAAGGAACACGCGGGTAGATGATTTCTAGCAGGCAGGAAGTCCTGTGCGGTGT
CACCATGAGCACCTCCAGCTGTACTAGTGCCATTGGAATAATAAATTTGATAAGGTGGTG
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_001961
Insert Size 3200 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001961.2.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001961.2, NP_001952.1
RefSeq Size 3148 bp
RefSeq ORF 2577 bp
Locus ID 1938
UniProt ID P13639
Domains EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV
Gene Summary This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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