United States
Germany
Japan
United Kingdom
China
GCLM (NM_002061) Human Untagged Clone
CAT＃: SC118877
GCLM (untagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLCLR |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118877 sequence for NM_002061 edited (data generated by NextGen Sequencing)
ATGGGCACCGACAGCCGCGCGGCCAAGGCGCTCCTGGCGCGGGCCCGCACCCTGCACCTG CAGACGGGGAACCTGCTGAACTGGGGCCGCCTGCGGAAGAAGTGCCCGTCCACGCACAGC GAGGAGCTTCATGATTGTATCCAAAAAACCTTGAATGAATGGAGTTCCCAAATCAACCCA GATTTGGTCAGGGAGTTTCCAGATGTCTTGGAATGCACTGTATCTCATGCAGTAGAAAAG ATAAATCCTGATGAAAGAGAAGAAATGAAAGTTTCTGCAAAACTGTTCATTGTAGAATCA AACTCTTCATCATCAACTAGAAGTGCAGTTGACATGGCCTGTTCAGTCCTTGGAGTTGCA CAGCTGGATTCTGTGATCATTGCTTCACCTCCTATTGAAGATGGAGTTAATCTTTCCTTG GAGCATTTACAGCCTTACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAAAAGATTGTT GCCATAGGTACCTCTGATCTAGACAAAACACAGTTGGAACAGCTGTATCAGTGGGCACAG GTAAAACCAAATAGTAACCAAGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTG ACTGCATTTGCTAAACAATTTGACATACAGCTGTTGACTCACAATGATCCAAAAGAACTG CTTTCTGAAGCAAGTTTCCAAGAAGCTCTTCAGGAAAGCATTCCTGACATTCAAGCGCAC GAGTGGGTGCCGCTGTGGCTACTGCGGTATTCGGTCATTGTGAAAAGTAGAGGAATTATC AAATCAAAAGGCTACATTTTACAAGCTAAAAGAAGGGGTTCTTAA Clone variation with respect to NM_002061.2 >OriGene 5' read for NM_002061 unedited
GGTTCAAATTTGTATACGACTCATATAGGCGGCCGCCGCGCGGCCGCCTGCCTGCCCGCC CGCCCGCAGCCCCTTCGCTGCGCGGCCCCTGGGCGGCCGCTGCCATGGGCACCGACAGCC GCGCGGCCAAGGCGCTCCTGGCGCGGGCCCGCACCCTGCACCTGCAGACGGGGAACCTGC TGAACTGGGGCCGCCTGCGGAAGAAGTGCCCGTCCACGCACAGCGAGGAGCTTCATGATT GTATCCAAAAAACCTTGAATGAATGGAGTTCCCAAATCAACCCAGATTTGGTCAGGGAGT TTCCAGATGTCTTGGAATGCACTGTATCTCATGCAGTAGAAAAGATAAATCCTGATGAAA GAGAAGAAATGAAAGTTTCTGCAAAACTGTTCATTGTAGAATCAAACTCTTCATCATCAA CTAGAAGTGCAGTTGACATGGCCTGTTCAGTCCTTGGAGTTGCACAGCTGGATTCTGTGA TCATTGCTTCACCTCCTATTGAAGATGGAGTTAATCTTTCCTTGGAGCATTTACAGCCTT ACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAAAAGATTGTTGCCATAGGTACCTCTG ATCTAGACAAAACACAGTTGGAACAGCTGTATCAGTGGGCACAGGTAAAACCAAATAGTA ACCAAGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCATTTGCTAAAC AATTTGACATACAGCTGTTGACTCACAATGATCCAAAAGAACTGCTTTCTGAAGCAAGTT CCAAGAAGCTCTTCAGAAAGCATTCCTGACATTCAGCGCACGAANTGGGTGCCGCTGTGG CTACTGGCGTATTTCGGTCATTGNGGAAAGTNNAGGAATATCAATCAAAGGCTACTTNTA CAGCTAAGAAAGGGGTCTNAACTGACTAGAGCATACTACTTGGAATNCCTCCATAGANAA A >OriGene 3' read for NM_002061 unedited
GGGGGCGGGNTGTCCTTCTCCCCCCCGGCAGATTTGAACCCGGCCGCATCTTATGATCGA TTTTTTTTTTTTTTTTTTGAAAAGGAGTCTCGCTTTGTCGCCCAGGCTGGAGTGCAGTGA TGTGATCTCGGCTCACTGTAACCTCCGCCTCCCGGGTTCAAGNGATTNTCCTGCCTTAGC CTCCCAAGTACCTGATATTACAAGCGAGTGCCACCACGCCCAGCCAATTTTTGTATTTTA GCCACAGATGCGGCCTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCGCGATC TGCCCACCTCCGCCCCACAAAGTGCTGGTATTACAGGTGCCAGCCACTGCGCCCGCACGA AATACAACAAATTTTCAAAAGAAAGATTTCACACTATTCAGCGTTACCACACCCCAATTT ATACACATAACGATACTTCATAGCGCACATCACCAGCTCATACCCTATCTATTCGCCAAT GTTGACGCCCCGAAAACCAATGCACCAATTAACTTACCTGACTTTTACATTTTTATTCCA CCTACAAACCTCTCACTCCTACCACTACTCCCCCTACAACACCCCACACCATTATCTTAA AACTCGTTCCACCTCTATAAAGTACTGACCCGCAATCAAGCAATTCTTTTTCACCCCCCC CATCTGGCCCTTAAACCCCTCCACAACCACCCCACCACACGGGGGAAAAATAAACCTACC ACACTTTCACTAAATAAGAATCTAAATTCCTACAATCTAATTGCCCCCTACCTTTTTCTC CAACGCTCTCTACGAATTCACAAACATCGCAACAAACCATTTTAATCTTCCCTCCACGCC TCTTATTCACCACTCACCCCAAACCAACACCCCTTCCATGTGCATCAACATATCATTATT AACCAAATNCCGGATATGCACCGTCCCATAGCACTCTCCTCCCCTTTACCACAACACTCC CTACAACTACTACCTTTTCATAAGAGCTGTCTAAGTCCTCTCTCCTCCAAANATAATAAC TCTTCGCCTATCTTCTCTCTCTCCCATCACGCCACCACTATCACACAATCAGATCGCGAC CTCAAACAATCTNAAAGTGTCCAATCTATATCACACACTCTTCATCAACTCTCTTAACCA TAATACACATCTNTCGGAATATACATCACACCTATCCCTCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002061 |
| Insert Size | 2630 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002061.2, NP_002052.1 |
| RefSeq Size | 3074 bp |
| RefSeq ORF | 825 bp |
| Locus ID | 2730 |
| UniProt ID | P48507 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glutathione metabolism, Metabolic pathways |
| Gene Summary | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207224 | GCLM (Myc-DDK-tagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM) |
CNY 3,600.00 |
|
| RC207224L1 | Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC207224L2 | Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), mGFP tagged |
CNY 5,890.00 |
|
| RC207224L3 | Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207224L4 | Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), mGFP tagged |
CNY 6,000.00 |
|
| RG207224 | GCLM (tGFP-tagged) - Human glutamate-cysteine ligase, modifier subunit (GCLM) |
CNY 5,200.00 |
