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GFPT1 (NM_002056) Human Untagged Clone
CAT＃: SC118874
GFPT1 (untagged)-Human glutamine--fructose-6-phosphate transaminase 1 (GFPT1)
CNY 5,000.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1L; MSLG |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118874 sequence for NM_002056 edited (data generated by NextGen Sequencing)
ATGTGTGGTATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCTG GAGACCCTAATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATTCTGCTGGTGTG GGATTTGATGGAGGCAATGATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATT AAGAAGAAAGGAAAAGTTAAGGCACTGGATGAAGAAGTTCACAAGCAACAAGATATGGAT TTGGATATAGAATTTGATGTACACCTTGGAATAGCTCATACCCGTTGGGCAACACATGGA GAACCCAGTCCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAATAATGAATTTATCGTT ATTCACAATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGGAAAGCAAAGGC TATGACTTCGAATCTGAAACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTAT GACAATCGGGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAACAA TTGGAAGGTGCTTTTGCACTTGTGTTTAAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGC ACAAGGCGAGGTAGCCCTCTGTTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGAT CACATTCCTATACTCTACAGAACAGGCAAAGACAAGAAAGGAAGCTGCAATCTCTCTCGT GTGGACAGCACAACCTGCCTTTTCCCGGTGGAAGAAAAAGCAGTGGAGTATTACTTTGCT TCTGATGCAAGTGCTGTCATAGAACACACCAATCGCGTCATCTTTCTGGAAGATGATGAT GTTGCAGCAGTAGTGGATGGACGTCTTTCTATCCATCGAATTAAACGAACTGCAGGAGAT CACCCCGGACGAGCTGTGCAAACACTCCAGATGGAACTCCAGCAGATCATGAAGGGCAAC TTCAGTTCATTTATGCAGAAGGAAATATTTGAGCAGCCAGAGTCTGTCGTGAACACAATG AGAGGAAGAGTCAACTTTGATGACTATACTGTGAATTTGGGTGGTTTGAAGGATCACATA AAGGAGATCCAGAGATGCCGGCGTTTGATTCTTATTGCTTGTGGAACAAGTTACCATGCT GGTGTAGCAACACGTCAAGTTCTTGAGGAGCTGACTGAGTTGCCTGTGATGGTGGAACTA GCAAGTGACTTCCTGGACAGAAACACACCAGTCTTTCGAGATGATGTTTGCTTTTTCCTT AGTCAATCAGGTGAGACAGCAGATACTTTGATGGGTCTTCGTTACTGTAAGGAGAGAGGA GCTTTAACTGTGGGGATCACAAACACAGTTGGCAGTTCCATATCACGGGAGACAGATTGT GGAGTTCATATTAATGCTGGTCCTGAGATTGGTGTGGCCAGTACAAAGGCTTATACCAGC CAGTTTGTATCCCTTGTGATGTTTGCCCTTATGATGTGTGATGATCGGATCTCCATGCAA GAAAGACGCAAAGAGATCATGCTTGGATTGAAACGGCTGCCTGATTTGATTAAGGAAGTA CTGAGCATGGATGACGAAATTCAGAAACTAGCAACAGAACTTTATCATCAGAAGTCAGTT CTGATAATGGGACGAGGCTATCATTATGCTACTTGTCTTGAAGGGGCACTGAAAATCAAA GAAATTACTTATATGCACTCTGAAGGCATCCTTGCTGGTGAATTGAAACATGGCCCTCTG GCTTTGGTGGATAAATTGATGCCTGTGATCATGATCATCATGAGAGATCACACTTATGCC AAGTGTCAGAATGCTCTTCAGCAAGTGGTTGCTCGGCAGGGGCGGCCTGTGGTAATTTGT GATAAGGAGGATACTGAGACCATTAAGAACACAAAAAGAACGATCAAGGTGCCCCACTCA GTGGACTGCTTGCAGGGCATTCTCAGCGTGATCCCTTTACAGTTGCTGGCTTTCCACCTT GCTGTGCTGAGAGGCTATGATGTTGATTTCCCACGGAATCTTGCCAAATCTGTGACTGTA GAGTGA Clone variation with respect to NM_002056.3 >OriGene 5' read for NM_002056 unedited
CACTCGGNGATTTGTNAAACCGACTTACTNATAGGNNCGGCCGCGCATTCGGCACGAGGC GGAGCCCGGGAGGCGGAGAAGGCTGTCGTTGCCTTGGCCGTCGCATCCCCGAGGGAGTCG TGTCGGCGCCACCCCGGCCCCCGAGCCCGCAGATTGCCCACCGAAGCTCGTGTGTGCACC CCCGATCCCGCCAGCCACTCGCCCCTGGCCTCGCGGGCCGTGTCTCCGGCATCATGTGTG GTATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCTGGAGACCC TAATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATTCTGCTGGTGTGGGATTTG ATGGAGGCAATGATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATTAAGAAGA AAGGAAAAGTTAAGGCACTGGATGAAGAAGTTCACAAGCAACAAGATATGGATTTGGATA TAGAATTTGATGTACACCTTGGAATAGCTCATACCCGTTGGGCAACACATGGAGAACCCA GTCCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAATAATGAATTTATCGTTATTCACA ATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGGAAAGCAAAGGCTATGACT TCGAATCTGANACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTATGACAATC GGGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAACAATTGGAAG GTGCTTTTGCACTTGTGTTTAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGCACAAGGCG AGGTAGCCCTCTGTTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGATCACATTCC TATACTCTACAGAACAGGCAAAGACAGA >OriGene 3' read for NM_002056 unedited
GGGGGGGTGGTTTNACAANNAAAATATAAAACCNGGGTGTTCTGTTNATTTNATAANCGT TCTGTGTTGTNGATTTAAANAACAGTAAAAATTGTCATTCAGCAGTCATTTTAAAAAATA AAAAACTACAGATACAAGGAAATAAAAAACACTTTTAGGAGATGAAAACACAAAGTAGTA GGATACCCAACATGTACACATCCCATCTTCAAATTTAAAATCATATTGTCAGTTGTCCAA AGCAGCTTGAATTTAAAGTTTGTGCTATAAAATTGTGCAAATATGTTAAGGATTGAGACC CACCAATGCACTACTGTAATATTTCGCTTCCTAAATTTCTTCCACCTACAGATAATAGAC AACAAGTCTGAGAAACTAAGGCTAACCAAACTTAGATATAAATCCTACCAATAAAATTTT TCAGTTTTAAGTTTTACAGTTTGATTTAAAAACAAAACAGAAACAAATTTCAAAATAAAT CACATCTTCTCTTAAAACTTGGCAAACCCTTCCCTAACTGTCCAAGTATGAGCATACACT GCCACTGGCTTTAGATACTCCAATTAAATGCACTACTCTTTCACTGGTCTGAATGAAGTA TGGTGAAACAAGTACCAATTTTTTAAAACATATTGTCTTTTTAAATTGAAGGGGAGGGGT CCAAAAATGGACAACCCAAATTTTTTTAAAAAAAAAAAAAGCTTAAAAATTTTTTTTGAT AACAAATTCCCAAATTGAAATTCCTTTTTTTTAAAAAGTTTTCCAACAATTTTCTGGGGA AAAATTAAAAGGGGGTTCTTTCAACAAAAAACAAAATTTTTTGGGGGGAATAAATTTAAC ACGGGGTGTTTTAAAAAAAGGGGTGGTCTTCAAAAAAAAGGCTCTTGAGGGGGGGGTTTT TTTTAAAAAAAGGGGTTTAATAAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002056 |
| Insert Size | 3460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002056.1, NP_002047.1 |
| RefSeq Size | 8649 bp |
| RefSeq ORF | 2046 bp |
| Locus ID | 2673 |
| UniProt ID | Q06210 |
| Domains | GATase_2, SIS |
| Protein Families | Protease |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Amino sugar and nucleotide sugar metabolism, Metabolic pathways |
| Gene Summary | This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hyaluronan production regulates metabolic and cancer stem-like properties of breast cancer cells via hexosamine biosynthetic pathway-coupled HIF-1 signaling
,Chanmee, T;Ontong, P;Izumikawa, T;Higashide, M;Mochizuki, N;Chokchaitaweesuk, C;Khansai, M;Nakajima, K;Kakizaki, I;Kongtawelert, P;Taniguchi, N;Itano, N;,
J. Biol. Chem.
,PubMed ID 27758869
[GFPT1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207225 | Myc-DDK-tagged ORF clone of Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1) as transfection-ready DNA |
CNY 7,480.00 |
|
| RC207225L1 | Lenti-ORF clone of Myc-DDK-tagged ORF clone of Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1) as transfection-ready DNA |
CNY 9,880.00 |
|
| RC207225L2 | Lenti-ORF clone of mGFP-tagged ORF clone of Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1) as transfection-ready DNA |
CNY 9,880.00 |
|
| RC207225L3 | Lenti-ORF clone of Myc-DDK-tagged ORF clone of Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1) as transfection-ready DNA |
CNY 6,940.00 |
|
| RC207225L4 | Lenti-ORF clone of mGFP-tagged ORF clone of Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1) as transfection-ready DNA |
CNY 9,880.00 |
|
| RG207225 | GFPT1 (tGFP-tagged) - Human glutamine--fructose-6-phosphate transaminase 1 (GFPT1) |
CNY 5,510.00 |
|
| SC333281 | GFPT1 (untagged) - Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2 |
CNY 6,560.00 |
