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Glutamine Synthetase (GLUL) (NM_002065) Human Untagged Clone
CAT＃: SC118847
GLUL (untagged)-Human glutamate-ammonia ligase (GLUL), transcript variant 1
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLNS; GS; PIG43; PIG59 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002065 edited
GAATTCGGCACGAGGGCGGAGCGTGTGAGCAGTACTGCGGCCTCCTCTCCTCTCCTAACC TCGCTCTCGCGGCCTAGCTTTACCCGCCCGCCTGCTCGGCGACCAGAACACCTTCCACCA TGACCACCTCAGCAAGTTCCCACTTAAATAAAGGCATCAAGCAGGTGTACATGTCCCTGC CTCAGGGTGAGAAAGTCCAGGCCATGTATATCTGGATCGATGGTACTGGAGAAGGACTGC GCTGCAAGACCCGGACCCTGGACAGTGAGCCCAAGTGTGTGGAAGAGTTGCCTGAGTGGA ATTTCGATGGCTCTAGTACTTTACAGTCTGAGGGTTCCAACAGTGACATGTATCTCGTGC CTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGACCCTAACAAGCTGGTGTTATGTGAAG TTTTCAAGTACAATCGAAGGCCTGCAGAGACCAATTTGAGGCACACCTGTAAACGGATAA TGGACATGGTGAGCAACCAGCACCCCTGGTTTGGCATGGAGCAGGAGTATACCCTCATGG GGACAGATGGGCACCCCTTTGGTTGGCCTTCCAACGGCTTCCCAGGGCCCCAGGGTCCAT ATTACTGTGGTGTGGGAGCAGACAGAGCCTATGGCAGGGACATCGTGGAGGCCCATTACC GGGCCTGCTTGTATGCTGGAGTCAAGATTGCGGGGACTAATGCCGAGGTCATGCCTGCCC AGTGGGAATTTCAGATTGGACCTTGTGAAGGAATCAGCATGGGAGATCATCTCTGGGTGG CCCGTTTCATCTTGCATCGTGTGTGTGAAGACTTTGGAGTGATAGCAACCTTTGATCCTA AGCCCATTCCTGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAGCACCAAGGCCA TGCGGGAGGAGAATGGTCTGAAGTACATCGAGGAGGCCATTGAGAAACTAAGCAAGCGGC ACCAGTACCACATCCGTGCCTATGATCCCAAGGGAGGCCTGGACAATGCCCGACGTCTAA CTGGATTCCATGAAACCTCCAACATCAACGACTTTTCTGCTGGTGTAGCCAATCGTAGCG CCAGCATACGCATTCCCCGGACTGTTGGCCAGGAGAAGAAGGGTTACTTTGAAGATCGTC GCCCCTCTGCCAACTGCGACCCCTTTTCGGTGACAGAAGCCCTCATCCGCACGTGTCTTC TCAATGAAACCGGCGATGAGCCCTTCCAGTACAAAAATTAAGTGGACTAGACCTCCAGCT GTTGAGCCCCTCCTAGTTCTTCATCCCACTCCAACTCTTCCCCCTCTCCCAGTTGTCCCG ATTGTAACTCAAAGGGTGGAATATCAAGGTCGTTTTTTTCATTCCAAAAAAAAAAAAAAA AAAAAAAACTCGAC >OriGene 5' read for NM_002065 unedited
ATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGAGCGTGTGAGC AGTACTGCGGCCTCCTCTCCTCTCCTAACCTCGCTCTCGCGGCCTAGCTTTACCCGCCCG CCTGCTCGGCGACCAGAACACCTTCCACCATGACCACCTCAGCAAGTTCCCACTTAAATA AAGGCATCAAGCAGGTGTACATGTCCCTGCCTCAGGGTGAGAAAGTCCAGGCCATGTATA TCTGGATCGATGGTACTGGAGAAGGACTGCGCTGCAAGACCCGGACCCTGGACAGTGAGC CCAAGTGTGTGGAAGAGTTGCCTGAGTGGAATTTCGATGGCTCTAGTACTTTACAGTCTG AGGGTTCCAACAGTGACATGTATCTCGTGCCTGCTGCCATGTTTCGGGACCCCTTCCGTA AGGACCCTAACAAGCTGGTGTTATGTGAAGTTTTCAAGTACAATCGAAGGCCTGCAGAGA CCAATTTGAGGCACACCTGTAAACGGATAATGGACATGGTGAGCAACCAGCACCCCTGGT TTGGCATGGAGCAGGAGTATACCCTCATGGGGACAGATGGGCACCCCTTTGGTTGGCCTT CCAACGGCTTCCCAGGGCCCCAGGGTCCATATTACTGTGGTGTGGGAGCAGACAGAGCCT ATGGCAGGGACATCGTGGAGGCCCATTACCGGGCCTGCTTGTATGCTGGAGTCAAGATTG CGGNGAACTAATGCCGAGTCATGCCTGCCCAGTGGGAATTTCAGATTGGACCTTGTGAAG GAATCAGCATGGGAGATCATCTCTGGGTGGCCCGTTTCATCTTGCATCGTGTGTGTGAAG AACTTGGAGTGATAGCACCC >OriGene 3' read for NM_002065 unedited
GACCGCGGCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTGGAATGAAAAA AACGACCTTGATATTCCACCCTTTGAGTTACAATCGGGACAACTGGGAGAGGGGGAAGAG TTGGAGTGGGATGAAAAACTAGGAGGGGCTCAACAGCTGGAGGTCTAGTCCACTTAATTT TTGTACTGGAAGGGCTCATCGCCGGTTTCATTGAGAAGACACGTGCGGATGAGGGCTTCT GTCACCGAAAAGGGGTCGCAGTTGGCAGAGGGGCGACGATCTTCAAAGTAACCCTTCTTC TCCTGGCCAACAGTCCGGGGAATGCGTATGCTGGCGCTACCATTGGCTACACCAGCAGAA AAGTCGTTGATGTTGGAGGTTTCATGGAATCCAGTTAGACGTCGGGCATTGTCCAGGCCT CCCTTGGGATCATAGGCACGGATGTGGTACTGGTGCCGCTTGCTTAGTTTCTCAATGGCC TCCTCGATGTACTTCAGACCATTCTCCTCCCGCATGGCCTTGGTGCTGAAGTTGGTATGG CAGCCTGCACCATTCCAGTTCCCAGGAATGGGCTTAGGATCAAAGGTTGCTATCACTCCA AAGTCTTCACACACACGATGCAAGATGAAACGGGCCACCCAGAGATGATCTNCCATGCTG ATTCCTTCACAAGGTCCAATCTGAAATTCCACTGGGCAGGCATGACCTCCGCATTAGTCC CCGCAATCTTGACTCCAGCATACAAGCAAGCCCGGTAATGGGCCCTCCACGATGTCCCTG GCCTAGGCTCTGCCTGCTCCACACCCCAGTAATTGGACCCTGGGGCCCTGGAAGCCCCTC GGAGGCCACCCAAGGGGTGCCATCTGGCCCACTGAGGGTACTCCTGTCCTGCCAAACACG GGGGCCGGTGGCTACCTGTCCTTATCCGTTACGGGGGCCTAAATGGGCTTGAGGCCTCAT TGGCCTGAAACTTCCATACACCACTGGTAGGGCCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002065 |
| Insert Size | 1430 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002065.4, NP_002056.2 |
| RefSeq Size | 3499 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 2752 |
| UniProt ID | P15104 |
| Domains | gln-synt, gln-synt_N |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) represents the longest transcript. Both variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Glutamine Acts as a Neuroprotectant against DNA Damage, Beta-Amyloid and H2O2-Induced Stress
,null,
PLoS ONE
,PubMed ID 22413000
[GLUL]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204161 | GLUL (Myc-DDK-tagged)-Human glutamate-ammonia ligase (GLUL), transcript variant 1 |
CNY 3,656.00 |
|
| RC204161L1 | Lenti ORF clone of Human glutamate-ammonia ligase (GLUL), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204161L2 | Lenti ORF clone of Human glutamate-ammonia ligase (GLUL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204161L3 | Lenti ORF clone of Human glutamate-ammonia ligase (GLUL), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204161L4 | Lenti ORF clone of Human glutamate-ammonia ligase (GLUL), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG204161 | GLUL (tGFP-tagged) - Human glutamate-ammonia ligase (GLUL), transcript variant 1 |
CNY 5,256.00 |
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