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Cytokeratin 8 (KRT8) (NM_002273) Human Untagged Clone
CAT＃: SC118727
KRT8 (untagged)-Human keratin 8 (KRT8)
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CARD2; CK-8; CK8; CYK8; K2C8; K8; KO |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118727 sequence for NM_002273 edited (data generated by NextGen Sequencing)
ATGTCCATCAGGGTGACCCAGAAGTCCTACAAGGTGTCCACCTCTGGCCCCCGGGCCTTC AGCAGCCGCTCCTACACGAGTGGGCCCGGTTCCCGCATCAGCTCCTCGAGCTTCTCCCGA GTGGGCAGCAGCAACTTTCGCGGTGGCCTGGGCGGCGGCTATGGTGGGGCCAGCGGCATG GGAGGCATCACCGCAGTTACGGTCAACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTG GACCCCAACATCCAGGCCGTGCGCACCCAGGAGAAGGAGCAGATCAAGACCCTCAACAAC AAGTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAGCAGAACAAGATGCTGGAG ACCAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGCAACATGGACAACATGTTC GAGAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAG CTGGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAG GATGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGTCCTCATCAAGAAGGATGTG GATGAAGCTTACATGAACAAGGTAGAGCTGGAGTCTCGCCTGGAAGGGCTGACCGACGAG ATCAACTTCCTCAGGCAGCTGTATGAAGAGGAGATCCGGGAGCTGCAGTCCCAGATCTCG GACACATCTGTGGTGCTGTCCATGGACAACAGCCGCTCCCTGGACATGGACAGCATCATT GCTGAGGTCAAGGCACAGTACGAGGATATTGCCAACCGCAGCCGGGCTGAGGCTGAGAGC ATGTACCAGATCAAGTATGAGGAGCTGCAGAGCCTGGCTGGGAAGCACGGGGATGACCTG CGGCGCACAAAGACTGAGATCTCTGAGATGAACCGGAACATCAGCCGGCTCCAGGCTGAG ATTGAGGGCCTCAAAGGCCAGAGGGCTTCCCTGGAGGCCGCCATTGCAGATGCCGAGCAG CGTGGAGAGCTGGCCATTAAGGATGCCAACGCCAAGTTGTCCGAGCTGGAGGCCGCCCTG CAGCGGGCCAAGCAGGACATGGCGCGGCAGCTGCGTGAGTACCAGGAGCTGATGAACGTC AAGCTGGCCCTGGACATCGAGATCGCCACCTACAGGAAGCTGCTGGAGGGCGAGGAGAGC CGGCTGGAGTCTGGGATGCAGAACATGAGTATTCATACGAAGACCACCAGCGGCTATGCA GGTGGTCTGAGCTCGGCCTATGGGGGCCTCACAAGCCCCGGCCTCAGCTACAGCCTGGGC TCCAGCTTTGGCTCTGGCGCGGGCTCCAGCTCCTTCAGCCGCACCAGCTCCTCCAGGGCC GTGGTTGTGAAGAAGATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTC CTGCCCAAGTGA Clone variation with respect to NM_002273.3 681 a=>g >OriGene 5' read for NM_002273 unedited
ACGGTCACGATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTTCTC CGCTCCTTCTAGGATCTCCGCCTGGTTCGGCCCGCCTGCCTCCACTCCTGCCTCCACCAT GTCCATCAGGGTGACCCAGAAGTCCTACAAGGTGTCCACCTCTGGCCCCCGGGCCTTCAG CAGCCGCTCCTACACGAGTGGGCCCGGTTCCCGCATCAGCTCCTCGAGCTTCTCCCGAGT GGGCAGCAGCAACTTTCGCGGTGGCCTGGGCGGCGGCTATGGTGGGGCCAGCGGCATGGG AGGCATCACCGCAGTTACGGTCAACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTGGA CCCCAACATCCAGGCCGTGCGCACCCAGGAGAAGGAGCAGATCAAGACCCTCAACAACAA GTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAGCAGAACAAGATGCTGGAGAC CAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGCAACATGGACAACATGTTCGA GAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAGCT GGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAGGA TGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGNCCTCATCAAGAAAGATGTGGA TGAAGCCTACATGAACAAGTAANAGCTGGAGTCTCGCCTGGAAGGCTGACCGACGAGATC ACTTCCTCCAGCAGCTGTATGAAGAAGAGATCCGGGNAGCTGCAGTCCCAGATCTCGGCA CATCTGTGGTGCTGTCCATGGACACAGCCGTTCCCTGGCATGGACAGCTCATTGCTTAGG TAAGCCCAA >OriGene 3' read for NM_002273 unedited
AGAGTCGAGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTCGACATTGGCAGAGCTAGCTGA GGTTTTATTTTGGACCAAAAAAAAAAAGCAATTGAATTGTTTTGTACCTGGAGGCATGGG CAAGGGGGGTCCCCAGGTAGTAAACTCCCCAGGTGGGCTGAGGGCTAAGGCTGAACCTCA GGTGGGTCTCCTGTTCCCAGTGCTACCCTGCATAGCGGCCTCCTTCCCAGGCTCTGGGGC AACGCAAGAGGGGTAGGCTGGGAGGGGCTGCCCCAACTGTTCACTTGGGCAGGACGTCAG AGGACTCAGACACCAGCTTCCCATCACGTGTCTCGATCTTCTTTACAACCACGGCCCTGG AGGAACTTGTGCGGCTGAAAGAGCTGGACCCCCCGCCAAAGCCAAAGCTGGACCCCAAGC TGTACCTGAAGCCCGGGCTTTTGAGGCCCCCATAGGCCGAGCTTAGACCACCTGCTTAGC CCGTGGGGGCATCCACTCTAAAACCTCTGCACCTGCCAACACACTACCAGCGAGCTCCTA ACACCGACACACCTCCATTTAGGCCGCCTTTTTTTCTTCCCCGCCCCATTTCCCCTTCCC CCTCTCGCTCCCCTCGTCTCCGCCTTCTCCCCCTTACCATCTCCCCCGTCCCCTCACCCC GTCTTCCCCCCCCCCGCTTCCCCTCCTTCTTTTTTCCCCTCTTCTCTCTCATTTACCACA CTCGTTTTCATTCCAATCTTCCGCCGACCACATATGCCAACCCTCATCTACACATTCCCC TACCGCCCGTCCATGCCCATCTCCACCCCCTCGTCGCCTCCCCTCCTCTTCCCCCTTTCA CATTCATGTCTTCTCCCGCTCGCGTCATTTCTCCCATTCTATCGCCTTCTCCCACCCCCT CTATCTTTCCTTTACCCTTGTTTCCTCCCCTCTCTCTGTTTCCGTTTTCTGCCCCTCCTC CCATACCGCGCTCGCGCTTTGCCGTCGCATATTCCACTGTTTCCCTCCTCCTCCCCATAC TCTTATACTCCCCCTCACCCTCCTCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002273 |
| Insert Size | 1880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002273.2, NP_002264.1 |
| RefSeq Size | 1788 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 3856 |
| UniProt ID | P05787 |
| Domains | filament |
| Protein Families | Druggable Genome |
| Gene Summary | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation in a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform 2. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209570 | KRT8 (Myc-DDK-tagged)-Human keratin 8 (KRT8) |
CNY 3,656.00 |
|
| RC209570L1 | Lenti ORF clone of Human keratin 8 (KRT8), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209570L2 | Lenti ORF clone of Human keratin 8 (KRT8), mGFP tagged |
CNY 5,890.00 |
|
| RC209570L3 | Lenti ORF clone of Human keratin 8 (KRT8), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209570L4 | Lenti ORF clone of Human keratin 8 (KRT8), mGFP tagged |
CNY 5,890.00 |
|
| RG209570 | KRT8 (tGFP-tagged) - Human keratin 8 (KRT8) |
CNY 5,256.00 |
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