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MEKK3 (MAP3K3) (NM_002401) Human Untagged Clone
CAT＃: SC118669
MAP3K3 (untagged)-Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2
CNY 4,672.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAPKKK3; MEKK3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002401 edited
ATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTCCAGATGAAC CGACGTCACCGGATGCCTGGATATGAGACCATGAAGAACAAAGACACAGGTCACTCAAAT AGGCAGAGTGACGTCAGAATCAAGTTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTC AGCCGGCCTGTGAAATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCT CTTGATCTACATTACATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAAGATGATCTT GATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGCTG TTGTCCCAGGACAGAAACCATAACAGTTCCTCTCCCCACTCTGGGGTGTCCAGACAGGTG CGGATCAAGGCTTCCCAGTCCGCAGGGGATATAAATACTATCTACCAGCCCCCCGAGCCC AGAAGCAGGCACCTCTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGC TATGTTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACCAGCATCAACAGT GAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTGAGCAGTGCA GAAAATTCCTTGTCTGGAAGCTGCCAATCCTTGGACAGGTCAGCAGACAGCCCATCCTTC CGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCTGACAACAGACAGGAATACTCAGAT CGGGAAACTCAGCTTTATGACAAAGGGGTCAAAGGTGGAACCTACCCCCGGCGCTACCAC GTGTCTGTGCACCACAAGGACTACAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGT CATCAAGGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGGCGAG AACATGGGTCTGGCTGTGCAATACCTGGACCCCCGTGGGCGCCTGCGGAGTGCGGACAGC GAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGTCTCCCAGTGCCCCCATC AACTGGCGCCGGGGAAAGCTCCTGGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTAT GACGTGGACACGGGACGTGAACTTGCTTCCAAGCAGGTCCAATTTGATCCAGACAGTCCT GAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAG CATGAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCGCGCTGAGAAGACCCTGACC ATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGTTGAAGGCTTACGGTGCT CTGACAGAGAGCGTGACCCGAAAGTACACGCGGCAGATCCTGGAGGGCATGTCCTACCTG CACAGCAACATGATTGTTCACCGGGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCT GGGAATGTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTATGTCG GGGACGGGCATGCGCTCCGTCACTGGCACACCCTACTGGATGAGCCCTGAGGTGATCAGC GGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCTGCACTGTGGTGGAGATG CTGACAGAGAAACCACCGTGGGCAGAGTATGAAGCTATGGCCGCCATCTTCAAGATTGCC ACCCAGCCCACCAATCCTCAGCTGCCCTCCCACATCTCTGAACATGTCCGGGACTTCCTG AGGCGCATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACACCAC TTTGCACAGCTCATGTACTGA >OriGene 5' read for NM_002401 unedited
AGGGTACAGTTGTTTACCACATACTAGAGGCGGAAAAACCTTCGGCACCAGGCCCCCCGC GTTTAGCCAGGCCCGCTGCCGTCCCCGCCGCCCGGGCCCCCGGCATGCAGCCCCGGCTGG GAGGTGACACTCACGGACCTTAGCCACCGCCGCCGCCATCGCCACCATGGACGAACAGGA GGCATTGAACTCAATCATGAACGATCTGGTGGTTTTCCAGATGAACCGACGTCACCGGAT GCCTGGATATGAGACCATGAAGAACAAAGACACAGGTCACTCAAATAGGCAGAGTGACGT CAGAATCAAGTTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTCAGCCGGCCTGTGAA ATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTCTTGATCTACATTA CATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAAGATGATCTTGATAAAGCAATTGA CATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGCTGTTGTCCCAGGACAG AAACCATAACAGTTCCTCTCCCCACTCTGGGGTGTCCAGACAGGTGCGGATCAAGGCTTC CCAGTCCGCAGGGGATATAAATACTATCTACCAGCCCCCCGAGCCCAGAAGCAGGCACCT CTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATGTTCCTGAGCG GCAGCAGCACATTGCCCCGCAGGGGTCCTACACCAGCATCAACAGTGAGGGGGAGTTCAT CCCCTAGACCAGCGAGCAGTGCTTGCTGGATCCCCTGACCAGTGCAGAAAAATCCTTGTC TGGAAGCTGCCAAATCTTGGACAAGTCACCAGACAGCCCATCCCTCCGGAAATCACGATG TCCCGTGCCCAG >OriGene 3' read for NM_002401 unedited
GGGGGGGNNNNNNNNNNNCNNNTTTNTNNTNNNNNNNNNATTACTTGAACCCGGCCGCAT ACTANGATCGGTTTTTTTTTTTTTTTTTTTCCTTTAATGACAACTCCCAGGGAACTGGGT GTTGAGAGGGAGGAGCTCTAGCAATAGGCACATTTTGGAGGGGAGGGGCCGGAAGAAAGA CCAGCGCCCAGGCTGAGGTAAATATGGCCAACTCTAAGCTGCTTGGAGATGCTGTTTCCC CAACCCCTGCTGCCATTCCCCCTCCAGGGGAGGAGCTACAAGGGAAAGGGGCTGAGACCT CAAGGGGATGGGCGAGGCTGGCTGAGGAAGAACAGAGCTGGCGGTTTTGGACACAGCTGG TGGTAGCATATGGGCTTTCCCTTCTACCAGATGGAGTGGGCTCTGGCCTAGCAGGTCACG ACTCAGGCAGCCTGAGACACCTCCTCGGGCTCTCTGGGATCAGGGCACTGGCCAGGCCAG ATGCCCCCACCGTGTCGTCCATCTCCCCTTCTGCTCCAGTGGGGAACAAAGGCTGGGCCA GGAGCGCAAAGGCCACAACCTTCAGCTCTTTTCAATGCAGCCTAGGCCCAAGCCCAAAGC AGGGGTACTCAGAGCCCAGAGGGCACACACAGTCTCCAGTTTAGAAACTGGGTACAGCAT AAGAGTGACTCCAACTTGTAAAACAGAGACAACTGCATNTGACAAACTGGATCCCCCCTC AGTGGGCAGCAGTTCACATCGCGGGCCCCTGCCCTGGANAGGCAGGGTCATGGGCACAAG CAGGACAAGGCTCTNCTTTCCCTTGATAGGCAGCATGACATTNGCTCATCCATCTTTGGA AAAGGACANGACTGGCAAACCCACCTGGGACTCTAGGGACGGGAACCTGGGCCTCTGCTG CCACTGCTACGAGTGCTGGCCTTTCCTCAGAGCTGTGGGACCTCATCCAGCCAGAGATGC CTGTTGGTCTNCAAACATGNGAAGGAAAACAATTACCCCTTGAATGAAATAACACTTTTA TC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002401 |
| Insert Size | 3460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002401.3, NP_002392.2 |
| RefSeq Size | 4750 bp |
| RefSeq ORF | 1881 bp |
| Locus ID | 4215 |
| UniProt ID | Q99759 |
| Domains | PB1, pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway |
| Gene Summary | This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210317 | MAP3K3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2 |
CNY 4,664.00 |
|
| RC210317L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2, Myc-DDK-tagged |
CNY 7,064.00 |
|
| RC210317L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC210317L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2, Myc-DDK-tagged |
CNY 7,064.00 |
|
| RC210317L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2, mGFP tagged |
CNY 7,064.00 |
|
| RG210317 | MAP3K3 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2 |
CNY 6,264.00 |
|
| SC323554 | MAP3K3 (untagged)-Kinase deficient mutant (K422M) of Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2 |
CNY 4,672.00 |
