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ME1 (NM_002395) Human Untagged Clone
CAT＃: SC118663
ME1 (untagged)-Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1)
CNY 6,400.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HUMNDME; MES |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118663 sequence for NM_002395 edited (data generated by NextGen Sequencing)
ATGGAGCCCGAAGCCCCCCGTCGCCGCCACACCCATCAGCGCGGCTACCTGCTGACACGG AACCCTCACCTCAACAAGGACTTGGCCTTTACCCTGGAAGAGAGACAGCAATTGAACATT CATGGATTGTTGCCACCTTCCTTCAACAGTCAGGAGATCCAGGTTCTTAGAGTAGTAAAA AATTTCGAGCATCTGAACTCTGACTTTGACAGGTATCTTCTCTTAATGGATCTCCAAGAT AGAAATGAAAAACTCTTTTATAGAGTGCTGACATCTGACATTGAGAAATTCATGCCTATT GTTTATACTCCCACTGTGGGTCTGGCTTGCCAACAATATAGTTTGGTGTTTCGGAAGCCA AGAGGTCTCTTTATTACTATCCACGATCGAGGGCATATTGCTTCAGTTCTCAATGCATGG CCAGAAGATGTCATCAAGGCCATTGTGGTGACTGATGGAGAGCGTATTCTTGGCTTGGGA GACCTTGGCTGTAATGGAATGGGCATCCCTGTGGGTAAATTGGCTCTATATACAGCTTGC GGAGGGATGAATCCTCAAGAATGTCTGCCTGTCATTCTGGATGTGGGAACCGAAAATGAG GAGTTACTTAAAGATCCACTCTACATTGGACTACGGCAGAGAAGAGTAAGAGGTTCTGAA TATGATGATTTTTTGGACGAATTCATGGAGGCAGTTTCTTCCAAGTATGGCATGAATTGC CTTATTCAGTTTGAAGATTTTGCCAATGTGAATGCATTTCGTCTCCTGAACAAGTATCGA AACCAGTATTGCACATTCAATGATGATATTCAAGGAACAGCATCTGTTGCAGTTGCAGGT CTCCTTGCAGCTCTTCGAATAACCAAGAACAAACTGTCTGATCAAACAATACTATTCCAA GGAGCTGGAGAGGCTGCCCTAGGGATTGCACACCTGATTGTGATGGCCTTGGAAAAAGAA GGTTTACCAAAAGAGAAAGCCATCAAAAAGATATGGCTGGTTGATTCAAAAGGATTAATA GTTAAGGGACGTGCTTCCTTAACACAAGAGAAAGAGAAGTTTGCCCATGAACATGAAGAA ATGAAGAACCTAGAAGCCATTGTTCAAGAAATAAAACCAACTGCCCTCATAGGAGTTGCT GCAATTGGTGGTGCATTCTCAGAACAAATTCTCAAAGATATGGCTGCCTTCAATGAACGG CCTATTATTTTTGCTTTGAGTAATCCAACTAGCAAAGCAGAATGTTCTGCAGAGCAGTGC TACAAAATAACCAAGGGACGTGCAATTTTTGCCAGTGGCAGTCCTTTTGATCCAGTCACT CTTCCAAATGGACAGACCCTATATCCTGGCCAAGGCAACAATTCCTATGTGTTCCCTGGA GTTGCTCTTGGTGTTGTGGCGTGTGGATTGAGGCAGATCACAGATAATATTTTCCTCACT ACTGCTGAGGTTATAGCTCAGCAAGTGTCAGATAAACACTTGGAAGAGGGTCGGCTTTAT CCTCCTTTGAATACCATTAGAGATGTTTCTCTGAAAATTGCAGAAAAGATTGTGAAAGAT GCATACCAAGAAAAGACAGCCACAGTTTATCCTGAACCGCAAAACAAAGAAGCATTTGTC CGCTCCCAGATGTATAGTACTGATTATGACCAGATTCTACCTGATTGTTATTCTTGGCCT GAAGAGGTGCAGAAAATACAGACCAAAGTTGACCAGTAG Clone variation with respect to NM_002395.4 >OriGene 5' read for NM_002395 unedited
AACATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCGCCGCCAC AGCTGCAGTCAGCACCGTCACCCCAGCAGCATCCGCCGCCTGCACCGCGCGTGCGGCCCG CCCCGGCCTGACCCCGCCGCCGAACCCGGCGCCAGCCATGGAGCCCGAAGCCCCCCGTCG CCGCCACACCCATCAGCGCGGCTACCTGCTGACACGGAACCCTCACCTCAACAAGGACTT GGCCTTTACCCTGGAAGAGAGACAGCAATTGAACATTCATGGATTGTTGCCACCTTCCTT CAACAGTCAGGAGATCCAGGTTCTTAGAGTAGTAAAAAATTTCGAGCATCTGAACTCTGA CTTTGACAGGTATCTTCTCTTAATGGATCTCCAAGATAGAAATGAAAAACTCTTTTATAG AGTGCTGACATCTGACATTGAGAAATTCATGCCTATTGTTTATACTCCCACTGTGGGTCT GGCTTGCCAACAATATAGTTTGGTGTTTCGGAAGCCAAGAGGTCTCTTTATTACTATCCA CGATCGAGGGCATATTGCTTCAGTTCTCAATGCATGGCCAGAAGATGTCATCAAGGCCAT TGTGGTGACTGATGGAGAGCGTATTCTTGGCTTGGGAGACCTTGGCTGTAATGGAATGGG CATCCCTGTGGGTAAATTGGCTCTATATACAGCTTGCGGAGGGATGAATCCTCAAGATGT CTGCCTGTCATTCTGGATGTGGGAACCGAAATGANGAGTTACTTNAAGACCACTCTACAT TGGACTACNGCAGAGAAGAAGTAGAAGTTCTGATATGATGATTNTTTGGACGAATTCATG GAGGCAGTTTCTTCCAGTATGGCATGAATTGCCTTATTCAGNTTGAAGAATTNTGCCAAT GTGAATGGCATTTCGTCTCCCTGAACAGTATCGAAAACAGTATTGCACATTNCATG >OriGene 3' read for NM_002395 unedited
CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGTTTTAAT GTTGGTTATGTCAATTTATTAGTAATTTTGGTCTTCCCATTAGTGTCCTGTATAAAAATG ATGAGTTTCTCCGTAGTACGTACAACAGCTTTTAAAGAGAACGTAGGCAGAATAATTCAG ACAGAAACCATAAATAACCAGAAGGCAAAGTGAAGCAAAATTGTCTCATGTGATGTTTAT CCCAATTTATATCGATATTCTTCTATCAATTTTTAGACTGTTAAAGTAAAATCTTAATCT AAGTGTCTTATGAATCATTATAAAAGATTCCAACCTTTAAAAATTATGAAAGGTTTAAAG ACCTCATTAATAGAGTTAGAAATGTTTGCTATTATCCTACTGGTCAACTTTGGTCTGTAT TTTCTGCACCTCTTCAGGCCAAGAATAACAATCAGGTAGAATCTGGTCATAATCAGTACT ATACATCTGGGAGCGGACAAATGCTTCTTTGTTTTGCGGTTCAGGATAAACTGTGGCTGT CTTTTCTTGGTATGCATCTTTCACAATCTTTTCTGCAATTTTCAGAGAAACATCTCTAAT GGTATTCAAAGGAGGATAAAGCCGACCCTCTTCCAAGTGTTTATCTGACACTTGCTGAGC TATAACCTCAGCAGTAGTGAGGAAAATATTATCTGTGATCTGCCTCAATCCACACGCCAC AACACCAAGAGCAACTCCAGGGAACACATANGAATTGGTGCCTTGGCCAGNATATAGGGT CTGTCCATTTGGAAGATGACTGGATCAAAGGACTGCCACTGGCAAAATTGCACGTCCCTT GGGTATTTGTAGCACTGCTCTGCAAACATTCTGTTTGCTAGNTGGATACTCAAACAAAAT AATAGCCGTCATTGAAGGCACCATACTTTTGGAATTGTCTGGGAAGCCCACCATGCGCAC TCTTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002395 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002395.2, NP_002386.1 |
| RefSeq Size | 2212 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 4199 |
| UniProt ID | P48163 |
| Domains | malic |
| Protein Pathways | Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism |
| Gene Summary | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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The HMGB1 protein induces a metabolic type of tumour cell death by blocking aerobic respiration
,Gdynia, G;Sauer, SW;Kopitz, J;Fuchs, D;Duglova, K;Ruppert, T;Miller, M;Pahl, J;Cerwenka, A;Enders, M;Mairbäurl, H;Kaminski, MM;Penzel, R;Zhang, C;Fuller, JC;Wade, RC;Benner, A;Chang-Claude, J;Brenner, H;Hoffmeister, M;Zentgraf, H;Schirmacher, P;Roth, W;,
Nat Commun
,PubMed ID 26948869
[ME1]
|
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Enhanced Gastrointestinal Expression of Cytosolic Malic Enzyme (ME1) Induces Intestinal and Liver Lipogenic Gene Expression and Intestinal Cell Proliferation in Mice
,Al-Dwairi, A;Brown, AR;Pabona, JM;Van, TH;Hamdan, H;Mercado, CP;Quick, CM;Wight, PA;Simmen, RC;Simmen, FA;,
PLoS ONE
,PubMed ID 25402228
[ME1]
|
|
Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion
,Emma Heart, Gary W. Cline, Leon P. Collis, Rebecca L. Pongratz, Joshua P. Gray, and Peter J. S. Smith,
Am J Physiol Endocrinol Metab, Jun 2009; 296: E1354 - E1362
[ME1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206820 | ME1 (Myc-DDK-tagged)-Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1) |
CNY 6,384.00 |
|
| RC206820L1 | Lenti ORF clone of Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1), Myc-DDK-tagged |
CNY 8,784.00 |
|
| RC206820L2 | Lenti ORF clone of Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1), mGFP tagged |
CNY 5,890.00 |
|
| RC206820L3 | Lenti ORF clone of Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1), Myc-DDK-tagged |
CNY 8,784.00 |
|
| RC206820L4 | Lenti ORF clone of Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1), mGFP tagged |
CNY 8,784.00 |
|
| RG206820 | ME1 (tGFP-tagged) - Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1) |
CNY 7,984.00 |
|
| SC320569 | ME1 (untagged)-Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1) |
CNY 6,400.00 |
