NDUFS4 (NM_002495) Human Untagged Clone

CAT#: SC118622

NDUFS4 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) (NDUFS4), nuclear gene encoding mitochondrial protein



  "NM_002495" in other vectors (7)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-NDUFS4 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AQDQ; CI-18; CI-18 kDa; CI-AQDQ; MC1DN1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118622 sequence for NM_002495 edited (data generated by NextGen Sequencing)
ATGGCGGCGGTCTCAATGTCAGTGGTACTGAGGCAGACGTTGTGGCGGAGAAGGGCAGTG
GCTGTAGCTGCCCTTTCCGTTTCCAGGGTTCCGACCAGGTCGTTGAGGACTTCCACATGG
AGATTGGCACAGGACCAGACTCAAGACACACAACTCATAACAGTTGATGAANNNNTGGAT
ATCACTACTTTAACTGGCGTTCCAGAAGAGCATATAAAAACTAGAAAAGTCAGGATCTTT
GTTCCTGCTCGCAATAACATGCAGTCTGGAGTAAACAACACAAAGAAATGGAAGATGGAG
TTTGATACCAGGGAGCGATGGGAAAATCCTTTGATGGGTTGGGCATCAACGGCTGATCCC
TTATCCAACATGGTTCTAACCTTCAGTACTAAAGAAGATGCAGTTTCCTTTGCAGAAAAA
AATGGATGGAGCTATGACATTGAAGAGAGGAAGGTTCCAAAACCCAAGTCCAAGTCTTAT
GGTGCAAACTTTTCTTGGAACAAAAGAACAAGAGTATCCACAAAATAG

Clone variation with respect to NM_002495.2
12 g=>c;172 a=>n;173 a=>n;174 a=>n;175 t=>n;198 a=>c;312 a=>g
>OriGene 5' read for NM_002495 unedited
TCAAATTTTGTATACGACNACTATAGGGCGGCCGCGAATTCGCACGAGGAGCAAGATGGC
GGCGGTCTCAATGTCAGTGGTACTGAGGCAGACGTTGTGGCGGAGAAGGGCAGTGGCTGT
AGCTGCCCTTTCCGTTTCCAGGGTTCCGACCAGGTCGTTGAGGACTTCCACATGGAGATT
GGCACAGGACCAGACTCAAGACACACAACTCATAACAGTTGATGAAAAATTGGTTTATCT
TGTACCACTGTGTTTTACATTCTGTGGTCTAGCCACACCAAGCCTGGAACTTGCTACTCT
TTTTCCTGCTTCAAGGCATTTGGAGTTCCTGTTCACTCTACCTGGATGCATTTTAACTAA
TCCTGAGGACTTCATCCGGTCACCATCTACCTATTCTTCAAATGGATATCACTACTTTAA
CTGGCGTTCCAGAAGAGCATATAAAAACTAGAAAAGTCAGGATCTTTGTTCCTGCTCGCA
ATAACATGCAGTCTGGAGTAAACAACACAAAGAAATGGAAGATGGAGTTTGATACCAGGG
AGCGATGGGAAAATCCTTTGATGGGTTGGGCATCAACGGCTGATCCCTTATCCAACATGG
TTCTAACCTTCAGTACTAAAGAAGATGCAGTTTCCTTTGCAGAAAAAAATGGATGGAGCT
ATGACATTGAAGAGAGGAAGGTTCCAAAACCCAAGTCCAAGTCTTATGGTGCAAACTTTT
CTTGGAACAAAAGAACCAGAGTATCCACAAAATAGGTTGGCACTGACTATATCTCTGCTT
GACTGTGAAATAAGTCAGCTATGCAGTATTTATAGTCCATGTATAATAAATACATCTCTT
AATCTCCTAATANNATGGNACCTTTNAACTACANNAAAAAAAAAAAAAAACTCGACTCTA
GATTGGGGNCCGCGTCATAGA
>OriGene 3' read for NM_002495 unedited
CGACGCTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAGTTTAAAGGTCCAATTTATT
AGGAGATTAAGAGATGTATTTATTATACATGGACTATAAATACTGCATAGCTGACTTTAT
TCACAGTCAAGCAGAGATATAGTCAGTGCCAACCTATTTTGTGGATACTCTTGTTCTTTT
GTTCCAAGAAAAGTTTGCACCATAAGACTTGGACTTGGGTTTTGGAACCTTCCTCTCTTC
AATGTCATAGCTCCATCCATTTTTTTCTGCAAAGGAAACTGCATCTTCTTTAGTACTGAA
GGTTAGAACCATGTTGGATAAGGGATCAGCCGTTGATGCCCAACCCATCAAAGGATTTTC
CCATCGCTCCCTGGTATCAAACTCCATCTTACATTTCTTTGTGTTGCTTACTCCAGACTG
CATGTTATTGCGAGCAGGAACAAAGATCCTGACTTTTCTAGTTTTTATATGCTCTTCTGG
AACGCCAGCTAAAGTAGTGATATGCATTTGAAGAATAGGCACATGGCGACCGGATGAAGT
CCTCAGGATTAGTTAAAATGCATCCAGGTACAGTGAACAGGAACTCCAAATGCCTTGAAG
CACGAAAAAGAGTACCAAGTCCCAGGCTTGGTGTGGCTAGACCACAGAATGTAAAAACAG
TGGTACAAGATAAACCAATTTTTCATCAACCTGTCTGAATTGCGTGTTTTGAGTCTGGTC
CTGGGCCATCTTCATGGGGAAATTCTCACCACCTGTCCGGAACCCGGAAACGAAAGGCAG
TACAGCCCTGCCCTTTTCGCCCAAAGTTGCCCTATCCCCGGACTTGAACCCCGCCTTTTG
CTCTGTGCCGAATCCGGCCGCCTTATGAGTCCATACAAATTGACGTATTAAAAGCTTGTT
TTTAAACCTCCACGAACCCTACGTCATTGTGTAGGGGGGGGTTACACATTGGAAAAG
Restriction Sites NotI-NotI     
ACCN NM_002495
Insert Size 820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002495.1, NP_002486.1
RefSeq Size 668 bp
RefSeq ORF 528 bp
Locus ID 4724
UniProt ID O43181
Domains ETC_CI_21
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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