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PHKA1 (NM_002637) Human Untagged Clone
CAT＃: SC118481
PHKA1 (untagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1
CNY 8,520.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHKA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002637 edited
TGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAAT ACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAGCGGTCGTTGACCTGTTGGC GGCGGCCGGGCGGGAGGCTGTGGGCCGCGGCGGTCTCCCGGGGTTGAGGGGAGGATGCAG GAGTCACACGCGGAGGCCGGACTGGACTAAGGGGGCAGGAGCGGCGCCTGGGGCGCTGCG GCGGGGCTCTAGACCGCGGGAAGCCAGTGCTAGCGCTGGCGCGTGGTGGGCCCCTGAGGC CGCCGGAGTCCGGAGAGGGACCGAAGGCTCCGGTGGCACCCGGGCTATTTCTCAGAGGAC AATTAGTAACGTGTCGCCATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTAC GCTCGACTGGTGCAACAGACCATCCTGTGCCATCAGAATCCAGTGACTGGCTTGCTTCCA GCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAATGTGTACAGCATCTTGGCTGTG TGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGATAAGGCAAAGGCC TATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCAGA CAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCA AAGTACAACACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAG TTGGATGCTACCTCTGTGTACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCAT ATCATCCACAGCCTAGATGAAGTCAATTTCATACAGAACCTTGTGTTTTACATTGAAGCT GCATATAAAACTGCTGACTTCGGGATATGGGAACGTGGAGACAAGACCAACCAAGGGATC TCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGGCAGCCCTGGAAGCATTAGATGAA CTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATGAA GTACAGCACTGCCAGTCTATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAG GTTGATGCTAGTCTACTCTCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAG TTGGTGGAGCTCACAAAACAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGT CGCTTTCTACGAGATGGATATAAAACTCCTAAAGAGGATCCCAATCGTCTGTACTATGAA CCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGTGAATGGCCATTGTTCTGGACATAC TTTATTCTTGATGGGGTCTTCAGTGGCAATGCAGAACAGGTTCAAGAATATAAAGAGGCT CTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACAGT GTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCC ATGGGGAAATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCA GAGGGATTTTTAGCCCCTGGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCG AAGCCCGATGTTGTGGTTCAAGTCTCCATTCTAGCTGAAACAGAAGAAATCAAGACCATT TTGAAGGACAAGGGAATTTACGTGGAGACCATTGCTGAGGTATACCCCATCAGAGTACAA CCAGCTCGTATTCTCAGCCACATTTATTCCAGCCTAGGATGCAACAATAGAATGAAACTC AGTGGACGACCCTACAGACACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTCGG AAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTG GACAACAAGATGATAGTGGAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGG CGGATGACAGGCCAGCCCACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGAT GGAACAAGCTTGAATTCAAGTATCCTGGCAGCACTCCGAAAAATGCAAGATGGGTATTTT GGTGGGGCAAGGGTTCAAACAGGTAAATTGTCAGAGTTTTTGACAACATCTTGTTGCACA CACTTGAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAAGATTATGATGAC AACTATGATTACCTGGAATCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATGCT CGCTGTGGTGATGAAGTTGCTCGTTATTTAGATCACCTTTTGGCGCACACTGCTCCCCAT CCTAAACTAGCCCCTACCTCACAGAAGGGAGGGCTAGATCGGTTCCAAGCTGCTGTGCAA ACAACCTGCGACTTAATGTCCTTGGTGACCAAGGCCAAGGAACTGCATGTACAGAATGTT CACATGTATCTTCCTACGAAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGAT TCACCTCATCCCCGACAGGAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCT AGAGACCAGTCTGGGGAGGTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCA AGCTTACAGGAACAAGCTGATATCCTCTATATGCTGTATACTATGAAAGGACCTGACTGG AACACTGAATTGTATAATGAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTAT GGCAAAGTGGGAGAAATTCGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGG AAGAAAGTGGAAGCACTTGATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTG ACAGTAGGACTTCCTCCAGAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAG GCGCTCACTCAGCTGATAGATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACA CAGGAAATAATGGTATATCTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAA ATGTTTCGACTTCGAATTGGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACTCC CTTCGATGCTCAGCTGAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATG AAGAATCTCCTGCATCACATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGT CCCACTGATTCAAATGTCAGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCA ACCAAAACAGAACGAACTGGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCACCTGGA ACCTCTATGACTCCAAGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAA GATAGTCGTCAAGGTCAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTT CCAGTTGGATTTTATCAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTT GAAGGGTTTGTCCTTCCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTC TCTGTTCATGTGGAGTCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTG GTTGAAGCCATCCTTGTCCTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGC ATCATTGCTGTGGAAAAAATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGAAA ACCCTTGGCGCAGATGATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTCTT CTGTATGACAGTGCACCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCC GCCACCTACGTGCAGGAGTTCCTGCCCCACAGCATCTGTGCCATGCAATGAGGGCTTTGG TTCCTGGCTTCTGGGAGCCTTTTGACAGCTGGTCCCTGCCTCGGTTGATTGTGCATGGAA CTAAAATGTTATTGCCTAATCACTCCAACCCTGCCCCTTTCTGTCCCATCCTTCCCAAGA AGAGAGAACTTTTTCGATAAACTAACTACTGTAGAAGAAGTGAACACTTACCTGGAGGCT CACCTTGCAGAACCAGTGACAATCTTATGAGTATAATGAACACTCAGCCAGGCCTGTCAT GATTGGCTTTATTTCTTTCATCATTCATAAAAGTTTGCATGTGTTTTTATTCTCTAGATC TGTTACCAATATAGTTTTCTAACTCCTGTTTGGGGAGCAAGTGTTAATAATAACTTATTC CTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTG AACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCT >OriGene 5' read for NM_002637 unedited
GTAATTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACCAGGCAGCGGTCGT TGACCTGTTGGCGGCGGCCGGGCGGGAGGCTGTGGGCCGCGGCGGTCTCCCGGGGTTGAG GGGAGGATGCAGGAGTCACACGCGGAGGCCGGACTGGACTAAGGGGGCAGGAGCGGCGCC TGGGGCGCTGCGGCGGGGCTCTAGACCGCGGGAAGCCAGTGCTAGCGCTGGCGCGTGGTG GGCCCCTGAGGCCGCCGGAGTCCGGAGAGGGACCGAAGGCTCCGGTGGCACCCGGGCTAT TTCTCAGAGGACAATTAGTAACGTGTCGCCATGAGGAGCCGGAGTAACTCCGGGGTCCGG CTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTGTGCCATCAGAATCCAGTGACT GGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAATGTGTACAGC ATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGAT AAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCAC TGCATGATCAGACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGAT AGCCTNCATGCAAAGTACAACACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGG GGACACCTGCAGTTGGATGCTACCTCTGTGTACCTGCTCTTCTTAGCCCAAATGACTGCC TCAGGACTCCATATCATCCACAGCCTAGATGAAGTCAATTTCATACAGGACCCTTGTGTT TTACATTGAAGCTGCATATAAAACTGCTGACTNCGGATATGGGAACGTGGAGACAAGACC ACCCAGGGATCTCANAGATGAGCCAGTTCAGTTGGATGGCAAAGCAGCCCTGAAGCATAG ATN >OriGene 3' read for NM_002637 unedited
NNNNTTTTACTCTGNACCGCGGNNCCGCATTTNANGATCGNGTTTTTTTTTTTTTTTTTT AGGNATAAGTTATTATTAACACTTGCTCCCCAAACAGGATTAGAAAACTATATTGGTAAC AGATCTAGAGAATAAAAACACATGCAAACTTTTATGAATGATGAAAGAAATAAAGCCAAT CATGACAGGCCTGGCTGAGTGTTCATTATACTCATAAGATTGTCACTGGTTCTGCAAGGT GAGCCTCCAGGTAAGTGTTCACTTCTTCTACAGTAGTTAGTTTATCGAAAAAGTTCTCTC TTCTTGGGAAGGATGGGACAGAAAGGGGCAGGGTTGGAGTGATTAGGCAATAACATTTTA GTTCCATGCACAATCAACCGAGGCAGGGACCAGCTGTCAAAAGGCTCCCAGAAGCCAGGA ACCAAAGCCCTCATTGCATGGCACAGATGCTGTGGGGCAGGAACTCCTGCACGTAGGTGG CGGCTGCCTTGGAGAGGTAGGTCATGGTGCCAAACCTGCCACTGGGTGCACTGTCATACA GAAGAGTACAGATGCCAGATGCGGGATCCTTTGCCAACATGGTATCATCTGCGCCAAGGG TTTTCTGTTCTTGAAGGAACAAGTCATTGGCAATATGCACTATTTTTTNCACAGCAATGA TGCTTCCGATGCTATGAATTTCAATATCTGCCAGCATGGTGAGGACAAGGATGGCTTCTA CCACCAGCTGACGGAACTCTTGCTGAAGTCACGATTCAGGACAGACTCCACATGAACAGA GAAATTAATCTCACCTGGAGTTATTTCTTTAGTGGTAGAGGAAAGCAAGGCAAACCCCTT AAAAATAAATCCCGGGACACTTTTGCAAAATTTCCCTACTTTTGGCAAAAACCCCCCGGA ACTTTTTTAGTGTCCCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002637 |
| Insert Size | 4710 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002637.1, NP_002628.1 |
| RefSeq Size | 4215 bp |
| RefSeq ORF | 3672 bp |
| Locus ID | 5255 |
| UniProt ID | P46020 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Insulin signaling pathway |
| Gene Summary | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211553 | PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 3,744.00 |
|
| RC211553L3 | Lenti-ORF clone of PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 14,730.00 |
|
| RC211553L4 | Lenti-ORF clone of PHKA1 (mGFP-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 14,730.00 |
|
| RG211553 | PHKA1 (tGFP-tagged) - Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1 |
CNY 5,344.00 |
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