PSMD1 (NM_002807) Human Untagged Clone
CAT#: SC118428
PSMD1 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1
CNY 6,984.00
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | P112; Rpn2; S1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118428 sequence for NM_002807 edited (data generated by NextGen Sequencing)
ATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACCACAGCTTAAG GAATTTGCACTACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGAAATTTCCGAG TCCGTAGACAAAATAGAGGTTTTATACGAAGATGAAGGTTTCCGGAGTCGGCAGTTTGCA GCCTTAGTGGCATCTAAAGTATTTTATCACCTGGGGGCTTTTGAGGAGTCTCTGAATTAT GCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACTCTGAATATGTGGAAACTATT ATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGATTTGCCTGAA GGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAATGTTCCAGCGA TGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGACACGAAGACTG GACGTCTTTGAAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTTAGCTTATAGC CTTAAGCTCTGCATGTCTTTAATGCAGAATAAACAGTTTCGGAATAAAGTACTAAGAGTT CTAGTTAAAATCTACATGAACTTGGAGAAACCTGATTTCATCAATGTTTGTCAGTGCTTA ATTTTCTTAGATGATCCTCAGGCTGTGAGTGATATCTTAGAGAAACTGGTAAAGGAAGAC AACCTCCTGATGGCATATCAGATTTGTTTTGATTTGTATGAAAGTGCTAGCCAGCAGTTT TTGTCATCTGTAATCCAGAATCTTCGAACTGTTGGCACCCCTATTGCTTCTGTGCCTGGA TCCACTAATACGGGTACTGTTCCGGGATCAGAGAAAGACAGTGACTCGATGGAAACAGAA GAAAAGACAAGCAGTGCATTTGTAGGAAAGACACCAGAAGCCAGTCCAGAGCCTAAGGAC CAGACTTTGAAAATGATTAAAATTTTAAGTGGTGAAATGGCTATTGAGTTACATCTGCAG TTCTTAATACGAAACAATAATACAGACCTCATGATTCTAAAAAACACAAAGGATGCAGTA CGGAATTCTGTATGTCATACTGCAACCGTTATAGCAAACTCTTTTATGCACTGTGGGACA ACCAGTGACCAGTTTCTTAGAGATAATTTGGAATGGTTAGCCAGAGCCACTAACTGGGCA AAATTTACTGCTACAGCCAGTTTGGGTGTAATTCATAAGGGTCATGAAAAAGAAGCATTA CAGTTAATGGCAACATACCTTCCCAAGGATACTTCTTCAGGATCAGCCTATCAGGAAGGT GGAGGTCTCTATGCACTAGGTCTTATTCATGCCAATCATGGTGGTGATATAATTGACTAT CTGCTTAATCAGCTTAAGAACGCCAGCAATGATATCGTTAGACACGGTGGCAGTCTGGGC CTTGGTTTGGCAGCCATGGGAACTGCACGTCAAGATGTTTATGATTTGCTAAAAACAAAC CTTTATCAGGATGATGCAGTAACAGGGGAAGCAGCTGGCCTGGCCCTAGGTTTGGTTATG TTGGGCTCTAAAAATGCTCAGGCTATTGAGGACATGGTTGGTTATGCACAAGAAACTCAA CATGAGAAGATTCTGCGTGGTCTTGCAGTTGGCATAGCTTTAGTAATGTATGGGAGGATG GAAGAGGCTGATGCTCTCATTGAATCTCTCTGTCGTGACAAGGACCCAATTCTTCGAAGG TCTGGAATGTATACTGTAGCCATGGCTTATTGTGGCTCTGGTAACAACAAAGCAATTCGA CGCCTGCTACATGTTGCTGTAAGTGATGTTAATGATGATGTCAGGAGGGCAGCAGTAGAA TCACTTGGGTTCATTCTATTCAGAACCCCTGAACAGTGCCCAAGTGTTGTCTCTTTGTTG TCAGAGAGTTACAACCCTCATGTGCGCTACGGAGCTGCAATGGCCTTGGGGATATGCTGT GCTGGTACAGGAAACAAGGAAGCCATTAATTTGCTAGAACCAATGACAAACGACCCCGTG AACTACGTGAGGCAAGGGGCACTCATAGCTTCAGCTCTCATCATGATCCAGCAGACTGAA ATCACTTGTCCAAAGGTGAATCAGTTCAGACAGCTGTATTCCAAAGTCATCAATGATAAG TATGATGATGTCATGGCCAAGTTTGGCGCTATTCTGGCCCAGGGCATACTGGATGCAGGT GGTCATAATGTCACAATCTCCTTGCAGTCCAGGACTGGGCATACTCATATGCCTTCTGTG GTTGGCGTCCTTGTATTTACCCAGTTTTGGTTCTGGTTTCCTCTTTCACACTTCCTGTCA TTGGCTTATACCCCTACCTGTGTCATTGGCCTTAACAAGGACTTAAAGATGCCGAAAGTT CAGTATAAATCGAACTGTAAACCATCCACATTTGCATATCCTGCCCCTCTGGAAGTACCA AAAGAAAAAGAAAAGGAAAAGGTTTCTACTGCTGTATTATCTATAACTGCCAAGGCTAAA AAGAAGGAAAAAGAAAAGGAAAAAAAGGAGGAGGAGAAAATGGAAGTGGATGAGGCAGAG AAAAAGGAGGAAAAAGAGAAGAAAAAAGAACCTGAGCCAAACTTCCAGTTATTGGATAAC CCAGCCCGAGTTATGCCTGCCCAGCTTAAGGTCCTAACCATGCCGGAGACCTGTAGATAC CAGCCTTTCAAACCACTCTCTATTGGAGGCATCATCATTCTGAAGGATACCAGTGAAGAC ATTGAGGAGCTGGTGGAACCTGTGGCAGCACATGGCCCAAAAATCGAGGAGGAGGAACAA GAGCCAGAACCCCCAGAACCATTTGAGTATATTGATGATTAA Clone variation with respect to NM_002807.3 1297 c=>t;2161 c=>t >OriGene 5' read for NM_002807 unedited
GGGTTCAAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCCTG AGGAGGCGACTGACTGAGCAGCGCACCCGGGGAGCAAGGAGGCGCGGTGAACTGAGCGGC CCCTGAGCTGACAGATACACTGCGCAGCTGGAACGGCGAGCGAGCCGACGGGCGAGTGAG GGGCGCAGCCATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACC ACAGCTTAAGGAATTTGCACTACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGA AATTTCCGAGTCCGTAGACAAAATAGAGGTTTTATACGAAGATGAAGGTTTCCGGAGTCG GCAGTTTGCAGCCTTAGTGGCATCTAAAGTATTTTATCACCTGGGGGCTTTTGAGGAGTC TCTGAATTATGCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACTCTGAATATGT GGAAACTATTATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGA TTTGCCTGAAGGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAAT GTTCCAGCGATGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGAC ACGAAGACTGGACGTCTTTGAAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTT AGCTTATAGCCTTAAGCTCTGCATGTCTTTAATGCAGAATANACAGTTTCGGAATAAAGT ACTAAGAGTNCTAGTTAAAATCTACATGAACTTGGAGAAACCTGATTTCATCAATGTTTG TCAGTGCTTAAATTTCTTAGATGATCCCTCAGCTGTGAGTGATATCTTAGAGAAACTGGT NAAGGAGAN >OriGene 3' read for NM_002807 unedited
AATCTCTGNCCGCGGCCGCATTCTANAGTCGGTTTTTTTTTTTTTTTTTTGTCCATCTGG AGATTTTATATTTATTTGAAAACCGGGGGGGGGGAAAGAGCACACTCAACAGTCATATAT CTTCTCTTCAACCAAAGACTTGGAGACAGGGTTCTTCAGGTCAGTGCAGAGGCAACGATA CATGCATTGAATAGCAGGTCTCGGCATGAATTTCCTCATATTCATTCCCAATATGAGCCT GGACAGTGGGCTTCAGATAAGCATGTGAGATCCTCTGGCCCTTAATCATCAATATACTCA AATGGTTCTGGGGGTTCTGGCTCTTGTTCCTCCTCCTCGATTTTTGGGCCATGTGCTGCC ACAGGTTCCACCAGCTCCTCAATGTCTTCACTGGTATCCTTCAGAATGATGATGCCTCCA ATACAGAGTGGTTTGAAAGGCTGGTATCTACAGGTCTCCGGCATGGTTAGGACCTTAAGC TGGGCAGGCATAACTCGGGCTGGGTTATTCAATAACTGGAAGTTTGGCTCAGGCTCTTTC TTCTTCTCTTTTTCCTCCTTTTTCTCTGCCTCATTCACTTCCATTTTCTCCTCCTCCTTT TTTTCCTTTTTTTTTTCCTTCTTTCTTACCTTGGCCGTTATAGATAATACAGCAGTAGAA ACCTTTTCCCTTTTTTTTTCTTCTGGTCCTTCCACACGGGCCAGATATGCAAAAGTGGCT GGTTTACACGTTCATCCATATCGACCTTTCCGCACTCTTTAAGCACTTTGCAAGGGCCAC TGACCAGGTAGGGGTATAAGCCCCACGCCCGCAATGCCGAAAGAAGAAACCCGACCCCTA ACTGGGTTACAACCAAGGCGCCCACCCCCAAAAGGCATATGCAATTGCCCCATNTCTGGA ATGCCCCGGAATTGTGACATTACGCACACCCGCATCCATTC |
Restriction Sites | NotI-NotI |
ACCN | NM_002807 |
Insert Size | 3200 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002807.2, NP_002798.2 |
RefSeq Size | 3227 bp |
RefSeq ORF | 2862 bp |
Locus ID | 5707 |
UniProt ID | Q99460 |
Domains | PC_rep |
Protein Pathways | Proteasome |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. There is evidence that this proteasome and its subunits interact with viral proteins, including those of coronaviruses. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Aug 2020] Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1). |
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Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210486 | PSMD1 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1 |
CNY 6,976.00 |
|
RC210486L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, Myc-DDK-tagged |
CNY 9,376.00 |
|
RC210486L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
RC210486L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
RC210486L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
RG210486 | PSMD1 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1 |
CNY 8,576.00 |