RAD1 (NM_002853) Human Untagged Clone

CAT#: SC118373

RAD1 (untagged)-Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1



  "NM_002853" in other vectors (6)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


RAD1 Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HRAD1; REC1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118373 sequence for NM_002853 edited (data generated by NextGen Sequencing)
ATGCCCCTTCTGACCCAACAGATCCAAGACGAGGATGATCAGTACAGCCTTGTGGCCAGC
CTTGACAACGTTAGGAATCTCTCCACTATCTTGAAAGCTATTCATTTCCGAGAACATGCC
ACGTGTTTCGCAACTAAAAATGGTATCAAAGTAACAGTGGAAAATGCAAAGTGTGTGCAA
GCAAATGCTTTTATTCAGGCTGGAATATTTCAGGAGTTTAAAGTTCAGGAAGAGTCTGTT
ACTTTTCGAATTAATTTAACTGTCCTTTTAGACTGTTTATCTATTTTTGGATCAAGTCCT
ATGCCAGGGACTTTAACTGCACTTCGAATGTGTTACCAAGGTTATGGTTACCCTTTGATG
CTGTTCCTGGAAGAAGGAGGAGTGGTGACAGTCTGCAAAATCAATACACAGGAACCTGAG
GAGACCCTGGACTTTGATTTCTGCAGCACCAATGTTATTAATAAAATTATTCTGCAGTCA
GAGGGGCTCCGTGAAGCATTTTCTGAATTGGATATGACGAGTGAAGTCCTACAAATTACC
ATGTCTCCTGACAAGCCTTATTTCAGGTTATCTACTTTTGGAAATGCAGGAAGTTCCCAC
CTTGACTATCCCAAAGATTCTGATTTGATGGAAGCATTTCATTGTAATCAGACCCAAGTC
AACAGATACAAGATTTCCTTACTGAAACCCTCTACAAAGGCATTAGTCCTATCTTGTAAG
GTATCTATTCGGACAGATAACAGAGGCTTCCTTTCATTACAGTATATGATTAGAAATGAA
GATGGACAAATATGTTTTGTGGAATATTACTGCTGCCCTGATGAAGAAGTTCCTGAATCT
GAGTCTTGA

Clone variation with respect to NM_002853.3
>OriGene 5' read for NM_002853 unedited
GGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTCCTTC
CGGTCTCGGTGGCGCGGCACGCGCGGCTCTCTAGGCCTCCTTCAGCTCTGTGGTGACGGT
GGCCGAGGTGGAGGGCCGGTCTGAAGAGTGGCGGGACTGGCTTCACTTCCTCCGCGGTTC
CTCGGAGCCGCCTCGCTCCTCTTCAGGGACTTTGCTGAGAAGGGCTCTCGGGCGTCCAGA
CCCCACCGCAAAGGTGTTTGGCGATCCGCCGAGAAGTTGTTGGCCCCAGGAGCATCCCTC
GGGGCCGAATGCGCAGTGGACGATGCCCCTTCTGACCCAACAGATCCAAGACGAGGATGA
TCAGTACAGCCTTGTGGCCAGCCTTGACAACGTTAGGAATCTCTCCACTATCTTGAAAGC
TATTCATTTCCGAGAACATGCCACGTGTTTCGCAACTAAAAATGGTATCAAAGTAACAGT
GGAAAATGCAAAGTGTGTGCAAGCAAATGCTTTTATTCAGGGGCATGGTATGGTGGCTCA
CACCTGTATTTCCAGCTGCTCAGGAGACCGAGGCTGAAGCACGAAGATCGCTTGAGCTCA
GGCATTAAAGACCAGCCTGGGCTGGAATATTTCAGGAGTTTAAAGTTCAGGAAGAGTCTG
TTACTTTTCGAATTAATTTAACTGTCCTTTTAGACTGTTTATCTATTTTTGGATCAAGTC
CTATGCCAGGGACTTTAACTGCACTTCGAATGTGTTACCAAGGTTATGGTTACCCTTTGA
TGCTGTTCCTGGGAAGAGGGAGAGTGGTGACAGTCTGCAAAATCAATACACACGAACCTG
AGGAGACCCTGGACTTTGATTTCTGCAGCACCAATGTTATTAATAAAAATATCCTGCAGT
CAGAGGGGCTCCGTGAAGCATTTCTGAT
Restriction Sites NotI-NotI     
ACCN NM_002853
Insert Size 2620 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002853.2, NP_002844.1
RefSeq Size 1776 bp
RefSeq ORF 849 bp
Locus ID 5810
UniProt ID O60671
Domains Rad1
Protein Families Druggable Genome
Gene Summary This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]
Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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