RFC4 (NM_002916) Human Untagged Clone
CAT#: SC118346
RFC4 (untagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | A1; RFC37 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118346 sequence for NM_002916 edited (data generated by NextGen Sequencing)
ATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGA GGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGG GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA GTGATTACAGACATTGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC TGTCAGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGT CATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCT GATAAACAGAAGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGAT GGTGCTGATGAACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCT CAGAATTGTTAA Clone variation with respect to NM_002916.3 798 c=>t >OriGene 5' read for NM_002916 unedited
TTTGTATACGACTCACTATGGGCGGCNCGCGAATTCGCACGAGGGACTGTGGGGACATCA GTGATCGTAAGTCTCCTGGGCCCGTTATTCTCAGATTAGGTGACGGAGCTAAGACTTCGA GACCATCTCGTCCTTTTTGTATCGCGGAAACCTGAGGAACGAGCCGGCGGCGGTGACCTG CACGAGAAGCCAGGCTAACTGGGTGAAGTACCATGCAAGCATTTCTTAAAGGTACATCCA TCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCG GAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGTGTGG ATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATCTTTAGAAGGAGCAG ATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAAAAACATCCACTATTTTGG CAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAAGAGTTCTTGAGTTAAATG CATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAA CTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATTCTGG ATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACGTACCATGGAGAAGG AGTCGAAAACCACCCCGATCTGTCTTATCTGTAACTATGTCAGTCGAATAATTGAACCCC TGACCTCTAGATGTTCAAAATTCCGCCTTCAGGCTCTGTCAGATAAATTCAACAGCAGCG ATTACTAGACATTGCCCAGAAGGAAATGTCAAAATAGGATGAGGGATAGCTATCTTGGTT AGGGTCGAGAGACTTAGAAAGCCTTCATTCTTCG >OriGene 3' read for NM_002916 unedited
TTTGGACGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTGGGCATTTTATTTTTATTACAACTTCATTATTTACAAAACCCCCCATCCAGA TATATTCACGTTAACAATTCTGAGATAACTGCTGCATCACAGTTGCACAAAGGCTGATGA GTTGCAAATGTTCATCAGCACCATCTGCTAGGCATTTGTCAACTTCCTACCAGAAAAATT TAAGAAATTAGCATCCTTCAGGTAGTTAAATGTTCCATTGACATGTGCAAAGTACCTACT TACGGCAAGTTTTTCTGGGATAATAAACTTCTGTTTATCAAATAAGTTATTTTCTACAAC CACATCATGGAGTTGATTGACCAGCTGAGTTGCTGCATGACCCTCATCTATTAAATCCTA TAATAAAAAAAACTTTTGGTATGATGACTTAATATTCCTTTCCCCAAAGTTAGTAAGCTG ACTTTACCTTGACCACAGCTTCTAATTTGTCAAAAGAGCCACTCTGACAGGCAGCAAATA CTCCATCAATTTTCTCAGCTGGTATTACCTAAGTAATTGAATGTTCGGTATTAAAGATGT TTCTAGGTGAGACTAGACATACACTCTGCCAACTGGCCCCCGTGCCCCCAGTCATTTCTG TTCCACACCTAAGTTCCATACTTTTCTGGGAAGGCTTTGGGAGAACGGAAGGGCCAGAGT TACTCCAGCCACCACTAAGCAACCAAGTGCTAACAACTGTGCTTTTAAAGCCCTTTTATG GCCTTGTGAACTTCCAAGTTTGGGCACACATGGATTATAGCCTGNTCTGGTCAGGGGATG AAGATTAAGTTTTCCCCCTAGCGCTTTACGATTCCTCAAACAGTGGCCAGATCACCACAA GACTGATTNCTTAAAGGGNAGTGCCTTCTCTCATCTCTCCTGTAAGATGCACTATGAGAT GGNTTAAAGTCCCA |
Restriction Sites | NotI-NotI |
ACCN | NM_002916 |
Insert Size | 2580 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002916.3, NP_002907.1 |
RefSeq Size | 1427 bp |
RefSeq ORF | 1092 bp |
Locus ID | 5984 |
UniProt ID | P35249 |
Domains | AAA, AAA |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | DNA replication, Mismatch repair, Nucleotide excision repair |
Gene Summary | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200426 | RFC4 (Myc-DDK-tagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1 |
CNY 3,656.00 |
|
RC200426L1 | Lenti ORF clone of Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC200426L2 | Lenti ORF clone of Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC200426L3 | Lenti ORF clone of Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200426L4 | Lenti ORF clone of Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG200426 | RFC4 (tGFP-tagged) - Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1 |
CNY 5,256.00 |
|
SC320866 | RFC4 (untagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1 |
CNY 3,656.00 |