RFC4 (NM_002916) Human Untagged Clone

CAT#: SC118346

RFC4 (untagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1



  "NM_002916" in other vectors (7)

CNY 3,656.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
RFC4 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms A1; RFC37
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118346 sequence for NM_002916 edited (data generated by NextGen Sequencing)
ATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGA
GGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGG
GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA
GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT
GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT
TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA
GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG
TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG
GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT
AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG
CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC
AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA
GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA
GTGATTACAGACATTGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC
TGTCAGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGT
CATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCT
GATAAACAGAAGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGAT
GGTGCTGATGAACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCT
CAGAATTGTTAA

Clone variation with respect to NM_002916.3
798 c=>t
>OriGene 5' read for NM_002916 unedited
TTTGTATACGACTCACTATGGGCGGCNCGCGAATTCGCACGAGGGACTGTGGGGACATCA
GTGATCGTAAGTCTCCTGGGCCCGTTATTCTCAGATTAGGTGACGGAGCTAAGACTTCGA
GACCATCTCGTCCTTTTTGTATCGCGGAAACCTGAGGAACGAGCCGGCGGCGGTGACCTG
CACGAGAAGCCAGGCTAACTGGGTGAAGTACCATGCAAGCATTTCTTAAAGGTACATCCA
TCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCG
GAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGTGTGG
ATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATCTTTAGAAGGAGCAG
ATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAAAAACATCCACTATTTTGG
CAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAAGAGTTCTTGAGTTAAATG
CATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAA
CTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATTCTGG
ATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACGTACCATGGAGAAGG
AGTCGAAAACCACCCCGATCTGTCTTATCTGTAACTATGTCAGTCGAATAATTGAACCCC
TGACCTCTAGATGTTCAAAATTCCGCCTTCAGGCTCTGTCAGATAAATTCAACAGCAGCG
ATTACTAGACATTGCCCAGAAGGAAATGTCAAAATAGGATGAGGGATAGCTATCTTGGTT
AGGGTCGAGAGACTTAGAAAGCCTTCATTCTTCG
>OriGene 3' read for NM_002916 unedited
TTTGGACGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTGGGCATTTTATTTTTATTACAACTTCATTATTTACAAAACCCCCCATCCAGA
TATATTCACGTTAACAATTCTGAGATAACTGCTGCATCACAGTTGCACAAAGGCTGATGA
GTTGCAAATGTTCATCAGCACCATCTGCTAGGCATTTGTCAACTTCCTACCAGAAAAATT
TAAGAAATTAGCATCCTTCAGGTAGTTAAATGTTCCATTGACATGTGCAAAGTACCTACT
TACGGCAAGTTTTTCTGGGATAATAAACTTCTGTTTATCAAATAAGTTATTTTCTACAAC
CACATCATGGAGTTGATTGACCAGCTGAGTTGCTGCATGACCCTCATCTATTAAATCCTA
TAATAAAAAAAACTTTTGGTATGATGACTTAATATTCCTTTCCCCAAAGTTAGTAAGCTG
ACTTTACCTTGACCACAGCTTCTAATTTGTCAAAAGAGCCACTCTGACAGGCAGCAAATA
CTCCATCAATTTTCTCAGCTGGTATTACCTAAGTAATTGAATGTTCGGTATTAAAGATGT
TTCTAGGTGAGACTAGACATACACTCTGCCAACTGGCCCCCGTGCCCCCAGTCATTTCTG
TTCCACACCTAAGTTCCATACTTTTCTGGGAAGGCTTTGGGAGAACGGAAGGGCCAGAGT
TACTCCAGCCACCACTAAGCAACCAAGTGCTAACAACTGTGCTTTTAAAGCCCTTTTATG
GCCTTGTGAACTTCCAAGTTTGGGCACACATGGATTATAGCCTGNTCTGGTCAGGGGATG
AAGATTAAGTTTTCCCCCTAGCGCTTTACGATTCCTCAAACAGTGGCCAGATCACCACAA
GACTGATTNCTTAAAGGGNAGTGCCTTCTCTCATCTCTCCTGTAAGATGCACTATGAGAT
GGNTTAAAGTCCCA
Restriction Sites NotI-NotI     
ACCN NM_002916
Insert Size 2580 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002916.3, NP_002907.1
RefSeq Size 1427 bp
RefSeq ORF 1092 bp
Locus ID 5984
UniProt ID P35249
Domains AAA, AAA
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways DNA replication, Mismatch repair, Nucleotide excision repair
Gene Summary The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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