RFC2 (NM_002914) Human Untagged Clone
CAT#: SC118345
RFC2 (untagged)-Human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RFC40 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118345 sequence for NM_002914 edited (data generated by NextGen Sequencing)
ATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGTGGAGGCCCAGGACTCTGACCCT GCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCACTACGAACTGCCGTGGGTTGAA AAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAATGAAGACACCGTGAGCAGGCTA GAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCATCATTGCGGGCCCTCCAGGAACC GGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCTGCTGGGCCCAGCACTCAAAGAT GCCATGTTGGAACTCAATGCTTCAAATGACAGCATGACCGACGGAGCCCAGCAAGCCTTG AGGAGAACCATGGAAATCTACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGCTTCG GATAAGATCATCGAGCCCATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCTGACC GACGCCCAGATCCTCACCAGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTACACT GATGACGGCCTAGAAGCCATCATCTTCACGGCCCAGGGAGACATGAGGCAGGCGCTGAAC AACCTGCAGTCCACCTTCTCAGGATTTGGCTTCATTAACAGTGAGAACGTGTTCAAGGTC TGTGACGAGCCCCACCCACTGCTGGTAAAGGAGATGATCCAGCACTGTGTGAATGCCAAC ATTGACGAAGCCTACAAGATTCTTGCTCACTTGTGGCATCTGGGCTACTCACCAGAAGAT ATCATTGGCAACATCTTTCGAGTGTGTAAAACTTTCCAAATGGCAGAATACCTGAAACTG GAGTTTATCAAGGAAATTGGATACACTCACATGAAAATAGCGGAAGGAGTGAACTCTCTT TTGCAGATGGCAGGCCTCCTGGCAAGGCTGTGTCAGAAGACAATGGCCCCGGTGGCCAGT TAG Clone variation with respect to NM_002914.3 >OriGene 5' read for NM_002914 unedited
NNGGTCGGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCTCGGGT GGCGGGAAGAGGAGGCGCGAGAATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGT GGAGGCCCAGGACTCTGACCCTGCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCA CTACGAACTGCCGTGGGTTGAAAAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAA TGAAGACACCGTGAGCAGGCTAGAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCAT CATTGCGGGCCCTCCAGGAACCGGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCT GCTGGGCCCAGCACTCAAAGATGCCATGTTGGAACTCAATGCTTCAAATGACAGGGGCAT TGACGTTGTGAGGAATAAAATTAAAATGTTTGCTCAACAAAAAGTCACTCTTCCCAAAGG CCGACATAAGATCATCATTCTGGATGAAGCAGACAGCATGACCGACGGAGCCCAGCAAGC CTTGAGGAGAACCATGGAAATCTACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGC TTCGGATAAGATCATCGAGCCCATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCT GACCGACGCCCAGATCCTCACCAGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTA CACTGATGACGGCCTAGAAGCCATCATCTTCACGGCCCAGGGAGACATGANGCAGGCGCT GAACAACCTGCAGTCCACCTTCTCAGGATTTGGCTTCATTAACAGTGAGAACGTGNTCAA GGTCTGTGACGAGCCCCACCCACTGCTGGGTAAGGNAGAGATCCAGCACTGTGTGAATGC CACA >OriGene 3' read for NM_002914 unedited
TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAGTCACGGGAGT TTATTTATTTAATTTTTTTCCCCAGATGGAGACTCTGTCGCCCAGGCTGGAGTGCAATGG TGTGATCTTGGCTCACTGCAACCTCCACCTCCCGGGCTCAAGCGATTCTCCCGCCACAGC CTCCCGAGTAGCTGGGATTACAGGTGCCCGCCACCACACCCAGCTAATTTTTATATTTTT AGTAAAGACAGGGTTTCCCCATGTTGGCCAGGCTGGTCTTGAACTTCTGACCTCAGGTGA TCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCTACCGTGCCCGGCC AGCCACTGGAGTTTAAAGGACAGTCATGTTGGCTCCAGCCTAAGGCGGCATTTTCCCCCA TCAGAAAGCCGCGGCTCCTGTACCTCAGAATAGGGCACCTGTAAGTCAGTCAGTGAAGTC TCTGCTCTAACTGGCCACCGGGGCCATTGTCTTCTGACACAGCCTTGCCAGGAGGCCTGC CATCTGCCAAAGAGAGTTCACTCCTTCCGCTATTTTCATGTGAGTGTATCCAATTTCCCT TGATAACTCCAGTTTCAGGTATTCTGCCATTTGGAAAGTTTTACACACCTCGAAGATGTT GCCAATGATATCTTCTGGTGAGTAGCCCAGATGCCACAAGTGAGCACGAATCTTGTAGGC TTCGTCAATGGTGCCATTCACACAGTGGCTGGATCATCTCCTTTACCAGCAGTGGGTGGG GCTCGTCACAAGACCTTGAACACGTTTTCACTGGTAATGAAGCCAAATCCTGAAAAGGTG GACCTGCACGTTGTCCACCGCCTGTCTCATGTCTTCCTGGGCCGCGAAAATGAAGGCGTT CTAAGCCGTCATCAGTGTAAGGACCCCTCTTCTTTCTGGAAACATTCAATAAGCTGGGGG AGGAACTGGCCGTCNGCCCACNTTTGGTACCCGGAGACTGTCCAANGGCACTGAATGGCC TCCAGATCTTATCN |
Restriction Sites | NotI-NotI |
ACCN | NM_002914 |
Insert Size | 1630 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002914.3, NP_002905.2 |
RefSeq Size | 1613 bp |
RefSeq ORF | 963 bp |
Locus ID | 5982 |
UniProt ID | P35250 |
Domains | AAA, AAA |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | DNA replication, Mismatch repair, Nucleotide excision repair |
Gene Summary | This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201138 | RFC2 (Myc-DDK-tagged)-Human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2 |
CNY 2,400.00 |
|
RC201138L3 | Lenti ORF clone of Human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201138L4 | Lenti ORF clone of Human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG201138 | RFC2 (tGFP-tagged) - Human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2 |
CNY 4,000.00 |