CD43 (SPN) (NM_003123) Human Untagged Clone
CAT#: SC118170
SPN (untagged)-Human sialophorin (SPN), transcript variant 2
CNY 3,656.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CD43; GALGP; GPL115; LSN |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118170 sequence for NM_003123 edited (data generated by NextGen Sequencing)
ATGGCCACGCTTCTCCTTCTCCTTGGGGTGCTGGTGGTAAGCCCAGACGCTCTGGGGAGC ACAACAGCAGTGCAGACACCCACCTCCGGAGAGCCTTTGGTCTCTACTAGCGAGCCCCTG AGCTCAAAGATGTACACCACTTCAATAACAAGTGACCCTAAGGCCGACAGCACTGGGGAC CAGACCTCAGCCCTACCTCCCTCAACTTCCATCAATGAGGGATCCCCTCTTTGGACTTCC ATTGGTGCCAGCACTGGTTCCCCTTTACCTGAGCCAACAACCTACCAGGAAGTTTCCATC AAGATGTCATCAGTGCCCCAGGAAACCCCTCATGCAACCAGTCATCCTGCTGTTCCCATA ACAGCAAACTCTCTAGGATCCCACACCGTGACAGGTGGAACCATAACAACGAACTCTCCA GAAACCTCCAGTAGGACCAGTGGAGCCCCTGTTACCACGGCAGCTAGCTCTCTGGAGACC TCCAGAGGCACCTCTGGACCCCCTCTTACCATGGCAACTGTCTCTCTGGAGACTTCCAAA GGCACCTCTGGACCCCCTGTTACCATGGCAACTGACTCTCTGGAGACCTCCACTGGGACC ACTGGACCCCCTGTTACCATGACAACTGGCTCTCTGGAGCCCTCCAGCGGGGCCAGTGGA CCCCAGGTCTCTAGCGTAAAACTATCTACAATGATGTCTCCAACGACCTCCACCAACGCA AGCACTGTGCCCTTCCGGAACCCAGATGAGAACTCACGAGGCATGCTGCCAGTGGCTGTG CTTGTGGCCCTGCTGGCGGTCATAGTCCTCGTGGCTCTGCTCCTGCTGTGGCGCCGGCGG CAGAAGCGGCGGACTGGGGCCCTCGTGCTGAGCAGAGGCGGCAAGCGTAACGGGGTGGTG GACGCCTGGGCTGGGCCAGCCCAGGTCCCTGAGGAGGGGGCCGTGACAGTGACCGTGGGA GGGTCCGGGGGCGACAAGGGCTCTGGGTTCCCCGATGGGGAGGGGTCTAGCCGTCGGCCC ACGCTCACCACTTTCTTTGGCAGACGGAAGTCTCGCCAGGGCTCCCTGGCGATGGAGGAG CTGAAGTCTGGGTCAGGCCCCAGCCTCAAAGGGGAGGAGGAGCCACTGGTGGCCAGTGAG GATGGGGCTGTGGACGCCCCAGCTCCTGATGAGCCCGAAGGGGGAGACGGGGCTGCCCCT TAA Clone variation with respect to NM_003123.3 >OriGene 5' read for NM_003123 unedited
NGGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGACCTGGC CCCAGTGCTGCGTCCTTATCAGCCGAGCCGGTCCCAGCTCTTGCTCCTGCCTGTTTGCCT GGAAATGGCCACGCTTCTCCTTCTCCTTGGGGTGCTGGTGGTAAGCCCAGACGCTCTGGG GAGCACAACAGCAGTGCAGACACCCACCTCCGGAGAGCCTTTGGTCTCTACTAGCGAGCC CCTGAGCTCAAAGATGTACACCACTTCAATAACAAGTGACCCTAAGGCCGACAGCACTGG GGACCAGACCTCAGCCCTACCTCCCTCAACTTCCATCAATGAGGGATCCCCTCTTTGGAC TTCCATTGGTGCCAGCACTGGTTCCCCTTTACCTGAGCCAACAACCTACCAGGAAGTTTC CATCAAGATGTCATCAGTGCCCCAGGAAACCCCTCATGCAACCAGTCATCCTGCTGTTCC CATAACAGCAAACTCTCTAGGATCCCACACCGTGACAGGTGGAACCATAACAACGAACTC TCCAGAAACCTCCAGTAGGACCAGTGGAGCCCCTGTTACCACGGCAGCTAGCTCTCTGGA GACCTCCAGAGGCACCTCTGGACCCCCTCTTACCATGGCAACTGTCTCTCTGGAGACTTC CAAAGGCACCTCTGGACCCCCTGTTACCATGGCAACTGACTCTCTGGAGACCTCCACTGG GACCACTGGACCCNCTGTTACCATGACAACTGGCTCTCTGGAGCCCTCCAGCGGGGCCAG TGGACCCCAGGTCTCTAGCGTAAAACTATCTACAATGATGTCTCCAACGACCTNCACCAA CGCAAGCACTGTGCCCTTCCCGAACCCAGATGAGAACTCACGAGGCATGCTGCCAGTGGC TGTGCTTGTGGCCCCTGCTGCNGTCATAGTCCT >OriGene 3' read for NM_003123 unedited
CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGAGAAAAAGGGGTGGATGAG GAGGGTTTATTCATTCAGTCCACAGACGTTGAGAGGTGCCAGCCCTGCCCTGCCCAGGCG CTAGTTGTGGGTGCTGGGTGAGGATGGCAGCAGGAAATCCAGGGCTGAGAAGCCTCATGA GGATTCAGAGTGAAGAGGTGGGTGAGGAGGCAAGGCGCGGAGGCTCATGTCTGTAATCTC AGCACTTTGGGAGGCTGAGGCAGGTAGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTA GCCAACATGGTGAAACCCCATCTCTACCAAAAATACAAAAAAGCAGCTGGGCATGGTGGT GGGTGCCTGTAATCTCAGTTACTCGGGAAGCTGAGGCAGGAGAATCGCCTGAACTTAGGA GGCAGAGGTTGCAGTGAACTGAGATCGTGCATTGCACTCCAGCCTGGGCGACAAAGCGAA ACTCTGTCTCAAAAGAAAAAAAAAAAGGAGATTCGGGTGAGGATCAGGGTGCCGGGTGGA AACATGGGAGCGAGGGGGCAGTGGGATGGTGAGAGGGAAGGTGCTGGAGGCTGGCTGAGA TTCCGGCCTCCAGCCTCACTATTCACCGACACTTAAGGGGCAGCCCCGTCTCCCCCTTCG GGCTCATCAGGAGCTGGGGCGTCCACAGCCCCATTCTCACTGGCCACCAGTGGCTCCTCC TCCCCTTTGAGGCTGGGGCCTGACCCAGACTTCAGCTCCNTCATCGCCAGGGAGCCCCTG GCGAGACTTCCGTCTGCCAAAGAAAGTGGTGAGCGTTGGCCGACGGCTAGAACCCTCCCC ATCGGGCAACCCATAGCCCTTGTCGCCCCCGGCACCTCCCCGGTCACTTGTACGGCCCCT TCTTAGGACCTCGGGGTTGGCCCGCCCCAGTCGTTCCCCACCCGTTACGCTTGCCCCCTT GCCCATACCAGGCCCCA |
Restriction Sites | NotI-NotI |
ACCN | NM_003123 |
Insert Size | 1880 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003123.2, NP_003114.1 |
RefSeq Size | 2288 bp |
RefSeq ORF | 1203 bp |
Locus ID | 6693 |
UniProt ID | P16150 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Cell adhesion molecules (CAMs) |
Gene Summary | This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Mass Spectrometry-Based Identification Of The Tumor Antigen UN1 as the Transmembrane CD43 Sialoglycoprotein
,Annamaria de Laurentiis, Marco Gaspari, Camillo Palmieri, Cristina Falcone, Enrico Iaccino, Giuseppe Fiume, Ornella Massa, Mariorosario Masullo,
Mol. Cell. Proteomics, May 2011; 10: M111.007898
[SPN]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204195 | SPN (Myc-DDK-tagged)-Human sialophorin (SPN), transcript variant 2 |
CNY 3,656.00 |
|
RC204195L1 | Lenti ORF clone of Human sialophorin (SPN), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204195L2 | Lenti ORF clone of Human sialophorin (SPN), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC204195L3 | Lenti ORF clone of Human sialophorin (SPN), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204195L4 | Lenti ORF clone of Human sialophorin (SPN), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG204195 | SPN (tGFP-tagged) - Human sialophorin (SPN), transcript variant 2 |
CNY 5,256.00 |
|
SC320913 | SPN (untagged)-Human sialophorin (SPN), transcript variant 2 |
CNY 3,656.00 |