SOAT 1 (SOAT1) (NM_003101) Human Untagged Clone
CAT#: SC118156
SOAT1 (untagged)-Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113
CNY 6,152.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118156 sequence for NM_003101 edited (data generated by NextGen Sequencing)
ATGGTGGGTGAAGAGAAGATGTCTCTAAGAAACCGGCTGTCAAAGTCCAGGGAAAATCCT GAGGAAGATGAAGACCAGAGAAACCCTGCAAAGGAGTCCCTAGAGACACCTAGTAATGGT CGAATTGACATAAAACAGTTGATAGCAAAGAAGATAAAGTTGACAGCAGAGGCAGAGGAA TTGAAGCCATTTTTTATGAAGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTC ATTGAAAAGTCAGCATCATTAGATAATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTT GAAGGAGAGAAAAACAACCATAGAGCGAAGGATTTGAGAGCACCTCCAGAACAAGGAAAG ATTTTTATTGCAAGGCGCTCTCTCTTAGATGAACTGCTTGAAGTGGACCACATCAGAACA ACATATCACATGTTTATTGCCCTCCTCATTCTCTTTATCCTCAGCACACTTGTAGTAGAT TACATTGATGAAGGAAGGCTGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAA TTTCCTACCGTTGTTTGGACCTGGTGGATCATGTTCCTGTCTACATTTTCAGTTCCCTAT TTTCTGTTTCAACATTGGGCCACTGGCTATAGCAAGAGTTCTCATCCGCTGATCCGTTCT CTCTTCCATGGCTTTCTTTTCATGATCTTCCAGATTGGAGTTCTAGGTTTTGGACCAACA TATGTTGTGTTAGCATATACACTGCCACCAGCTTCCCGGTTCATCATTATATTCGAGCAG ATTCGTTTTGTAATGAAGGCCCACTCATTTGTCAGAGAGAACGTGCCTCGGGTACTAAAT TCAGCTAAGGAGAAATCAAGCACTGTTCCAATACCTACAGTCAACCAGTATTTGTACTTC TTATTTGCTCCTACCCTTATCTACCGTGACAGCTATCCCAGGAATCCCACTGTAAGATGG GGTTATGTTGCTATGAAGTTTGCACAGGTCTTTGGTTGCTTTTTCTATGTGTACTACATC TTTGAAAGGCTTTGTGCCCCCTTGTTTCGGAATATCAAACAGGAGCCCTTCAGCGCTCGT GTTCTGGTCCTATGTGTATTTAACTCCATCTTGCCAGGTGTGCTGATTCTCTTCCTTACT TTTTTTGCCTTTTTGCACTGCTGGCTCAATGCCTTTGCTGAGATGTTACGCTTTGGTGAC AGGATGTTCTATAAGGATTGGTGGAACTCCACGTCATACTCCAACTATTATAGAACCTGG AATGTGGTGGTCCATGACTGGCTATATTACTATGCTTACAAGGACTTTCTCTGGTTTTTC TCCAAGAGATTCAAATCTGCTGCCATGTTAGCTGTCTTTGCTGTATCTGCTGTAGTACAC GAATATGCCTTGGCTGTTTGCTTGAGCTTTTTCTATCCCGTGCTGTTCGTGCTCTTCATG TTCTTTGGAATGGCTTTCAACTTCATTGTCAATGATAGTCGGAAAAAGCCGATTTGGAAT GTTCTGATGTGGACTTCTCTTTTCTTGGGCAATGGAGTCTTACTCTGCTTTTATTCTCAA GAATGGTATGCACGTCGGCACTGTCCTCTGAAAAATCCCACATTTTTGGATTATGTCCGG CCACGTTCCTGGACTTGTCGTTACGTGTTTTAG Clone variation with respect to NM_003101.4 422 t=>c;969 c=>t;1425 c=>g;1577 a=>g >OriGene 5' read for NM_003101 unedited
TTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCTGGGCGCCAGGAGAGC TTCCCGGAGTCGACCTTCCTGCTGGCTGCTCTGTGACCGCTTCCCGGCTCTGCCCTCTTG GCCGAAGTGCCCGCTGCCGGGCGCGGGCCTCAGACAATACAATGGTGGGTGAAGAGAAGA TGTCTCTAAGAAACCGGCTGTCAAAGTCCAGGGAAAATCCTGAGGAAGATGAAGACCAGA GAAACCCTGCAAAGGAGTCCCTAGAGACACCTAGTAATGGTCGAATTGACATAAAACAGT TGATAGCAAAGAAGATAAAGTTGACAGCAGAGGCAGAGGAATTGAAGCCATTTTTTATGA AGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTCATTGAAAAGTCAGCATCAT TAGATAATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTTGAAGGAGAGAAAAACAACC ATAGAGCGAAGGATTTGAGAGCACCTCCAGAACAAGGAAAGATTTTTATTGCAAGGCGCT CTCTCTTAGATGAACTGCTTGAAGTGGACCACATCAGAACAACATATCACATGTTTATTG CCCTCCTCATTCTCTTTATCCTCAGCACACTTGTAGTAGATTACATTGATGAAGGAAGGC TGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAATTTCCTACCGTTGTTTGGA CCTGGTGGATCATGTTCCTGTCTAATTTTCAGTTCCCTATTTTCTGTTTCACATTGGGCC ACTGGCTATAGNNCAGAGTCTCATCCGCTGATCCGTTCTCTCTTCATGGCTNTCTTTTCA TGATCTTCCANATGGNAGTTCTAGGTTNTGG >OriGene 3' read for NM_003101 unedited
AGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTCCTTCCCAAAAAAGTCATATTTATAGTATTTCTTAACAGACATAT AAACCTAAAAATTCCATTCTCTAAATTCAAAAAATTGGCTTGCTCATAACCAGGTCTCAG CGCAATCTGTCCCCAATATATTTAAAATGCAGTTATAAATTCAAAATAAAAATTCGTTTT AATTTTAAAAAAAAATGTCAAGAATTACAAGCCATATTAAAGCAAACTAGTTAGCAGGTT ATGATTCCATTACAATTACAATACTGTTGTGCAAAACATTACTGCGGGAACATGCAAAGT ACCTTTTTAAAAATAACTTCCCTTGAGTGTATTTGTTTCCGTTGAAGTTGGATTTAATGT TTGAAAATTACCTAAACTCATCTGGACCAGTGGTATCGTGAGTAAAATACGTGATTAAGC TGGATACTTGGGGTTCAAATTAAGTTGGGACTATTAAAAAACTCTACTTACCTTCTTGTG AGACTCAAACTGACTTTAAAACAATTCCAAACAATACCAAGAGAGCCTCTCAAGTGGCTG TTCCAAGTAACAACTATCATTTAAATAATTCATAAATCTTACTTTGAGTGGAAAGTTGAA CTACATGGTCTCTGATACCCCTTCCATGCTCAATCTTTACAATCATACCTCATACATACA AATAATTTCTACACACTTGTATTAGAAATGGGCCTCTTATAAAATGGAAGTTTTACCTAA TAGAGTACATTTCCCTTGAACCCAATCCATATTAATTTTTCCCCCCTAAAACATCCACCC TTAAAAGTTAACTGGACAATGCCGAACCCCGCTTCTGGGATCTTAGGATCCCTTAATATG AGGGGAGGGATTAAACTTAGGTACGAACTCCCATCTGCAAAAAATTA |
Restriction Sites | NotI-NotI |
ACCN | NM_003101 |
Insert Size | 4000 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003101.4, NP_003092.4 |
RefSeq Size | 3407 bp |
RefSeq ORF | 1653 bp |
Locus ID | 6646 |
UniProt ID | P35610 |
Domains | MBOAT |
Protein Families | Transmembrane |
Protein Pathways | Steroid biosynthesis |
Gene Summary | The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205774 | SOAT1 (Myc-DDK-tagged)-Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113 |
CNY 6,144.00 |
|
RC205774L1 | Lenti ORF clone of Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113, Myc-DDK-tagged |
CNY 8,544.00 |
|
RC205774L2 | Lenti ORF clone of Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113, mGFP tagged |
CNY 5,890.00 |
|
RC205774L3 | Lenti ORF clone of Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205774L4 | Lenti ORF clone of Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113, mGFP tagged |
CNY 5,890.00 |
|
RG205774 | SOAT1 (tGFP-tagged) - Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113 |
CNY 7,744.00 |