SOAT 1 (SOAT1) (NM_003101) Human Untagged Clone

CAT#: SC118156

SOAT1 (untagged)-Human sterol O-acyltransferase 1 (SOAT1), nuclear gene encoding mitochondrial protein, transcript variant 688113



  "NM_003101" in other vectors (6)

CNY 6,152.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
SOAT1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118156 sequence for NM_003101 edited (data generated by NextGen Sequencing)
ATGGTGGGTGAAGAGAAGATGTCTCTAAGAAACCGGCTGTCAAAGTCCAGGGAAAATCCT
GAGGAAGATGAAGACCAGAGAAACCCTGCAAAGGAGTCCCTAGAGACACCTAGTAATGGT
CGAATTGACATAAAACAGTTGATAGCAAAGAAGATAAAGTTGACAGCAGAGGCAGAGGAA
TTGAAGCCATTTTTTATGAAGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTC
ATTGAAAAGTCAGCATCATTAGATAATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTT
GAAGGAGAGAAAAACAACCATAGAGCGAAGGATTTGAGAGCACCTCCAGAACAAGGAAAG
ATTTTTATTGCAAGGCGCTCTCTCTTAGATGAACTGCTTGAAGTGGACCACATCAGAACA
ACATATCACATGTTTATTGCCCTCCTCATTCTCTTTATCCTCAGCACACTTGTAGTAGAT
TACATTGATGAAGGAAGGCTGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAA
TTTCCTACCGTTGTTTGGACCTGGTGGATCATGTTCCTGTCTACATTTTCAGTTCCCTAT
TTTCTGTTTCAACATTGGGCCACTGGCTATAGCAAGAGTTCTCATCCGCTGATCCGTTCT
CTCTTCCATGGCTTTCTTTTCATGATCTTCCAGATTGGAGTTCTAGGTTTTGGACCAACA
TATGTTGTGTTAGCATATACACTGCCACCAGCTTCCCGGTTCATCATTATATTCGAGCAG
ATTCGTTTTGTAATGAAGGCCCACTCATTTGTCAGAGAGAACGTGCCTCGGGTACTAAAT
TCAGCTAAGGAGAAATCAAGCACTGTTCCAATACCTACAGTCAACCAGTATTTGTACTTC
TTATTTGCTCCTACCCTTATCTACCGTGACAGCTATCCCAGGAATCCCACTGTAAGATGG
GGTTATGTTGCTATGAAGTTTGCACAGGTCTTTGGTTGCTTTTTCTATGTGTACTACATC
TTTGAAAGGCTTTGTGCCCCCTTGTTTCGGAATATCAAACAGGAGCCCTTCAGCGCTCGT
GTTCTGGTCCTATGTGTATTTAACTCCATCTTGCCAGGTGTGCTGATTCTCTTCCTTACT
TTTTTTGCCTTTTTGCACTGCTGGCTCAATGCCTTTGCTGAGATGTTACGCTTTGGTGAC
AGGATGTTCTATAAGGATTGGTGGAACTCCACGTCATACTCCAACTATTATAGAACCTGG
AATGTGGTGGTCCATGACTGGCTATATTACTATGCTTACAAGGACTTTCTCTGGTTTTTC
TCCAAGAGATTCAAATCTGCTGCCATGTTAGCTGTCTTTGCTGTATCTGCTGTAGTACAC
GAATATGCCTTGGCTGTTTGCTTGAGCTTTTTCTATCCCGTGCTGTTCGTGCTCTTCATG
TTCTTTGGAATGGCTTTCAACTTCATTGTCAATGATAGTCGGAAAAAGCCGATTTGGAAT
GTTCTGATGTGGACTTCTCTTTTCTTGGGCAATGGAGTCTTACTCTGCTTTTATTCTCAA
GAATGGTATGCACGTCGGCACTGTCCTCTGAAAAATCCCACATTTTTGGATTATGTCCGG
CCACGTTCCTGGACTTGTCGTTACGTGTTTTAG

Clone variation with respect to NM_003101.4
422 t=>c;969 c=>t;1425 c=>g;1577 a=>g
>OriGene 5' read for NM_003101 unedited
TTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCTGGGCGCCAGGAGAGC
TTCCCGGAGTCGACCTTCCTGCTGGCTGCTCTGTGACCGCTTCCCGGCTCTGCCCTCTTG
GCCGAAGTGCCCGCTGCCGGGCGCGGGCCTCAGACAATACAATGGTGGGTGAAGAGAAGA
TGTCTCTAAGAAACCGGCTGTCAAAGTCCAGGGAAAATCCTGAGGAAGATGAAGACCAGA
GAAACCCTGCAAAGGAGTCCCTAGAGACACCTAGTAATGGTCGAATTGACATAAAACAGT
TGATAGCAAAGAAGATAAAGTTGACAGCAGAGGCAGAGGAATTGAAGCCATTTTTTATGA
AGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTCATTGAAAAGTCAGCATCAT
TAGATAATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTTGAAGGAGAGAAAAACAACC
ATAGAGCGAAGGATTTGAGAGCACCTCCAGAACAAGGAAAGATTTTTATTGCAAGGCGCT
CTCTCTTAGATGAACTGCTTGAAGTGGACCACATCAGAACAACATATCACATGTTTATTG
CCCTCCTCATTCTCTTTATCCTCAGCACACTTGTAGTAGATTACATTGATGAAGGAAGGC
TGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAATTTCCTACCGTTGTTTGGA
CCTGGTGGATCATGTTCCTGTCTAATTTTCAGTTCCCTATTTTCTGTTTCACATTGGGCC
ACTGGCTATAGNNCAGAGTCTCATCCGCTGATCCGTTCTCTCTTCATGGCTNTCTTTTCA
TGATCTTCCANATGGNAGTTCTAGGTTNTGG
>OriGene 3' read for NM_003101 unedited
AGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTCCTTCCCAAAAAAGTCATATTTATAGTATTTCTTAACAGACATAT
AAACCTAAAAATTCCATTCTCTAAATTCAAAAAATTGGCTTGCTCATAACCAGGTCTCAG
CGCAATCTGTCCCCAATATATTTAAAATGCAGTTATAAATTCAAAATAAAAATTCGTTTT
AATTTTAAAAAAAAATGTCAAGAATTACAAGCCATATTAAAGCAAACTAGTTAGCAGGTT
ATGATTCCATTACAATTACAATACTGTTGTGCAAAACATTACTGCGGGAACATGCAAAGT
ACCTTTTTAAAAATAACTTCCCTTGAGTGTATTTGTTTCCGTTGAAGTTGGATTTAATGT
TTGAAAATTACCTAAACTCATCTGGACCAGTGGTATCGTGAGTAAAATACGTGATTAAGC
TGGATACTTGGGGTTCAAATTAAGTTGGGACTATTAAAAAACTCTACTTACCTTCTTGTG
AGACTCAAACTGACTTTAAAACAATTCCAAACAATACCAAGAGAGCCTCTCAAGTGGCTG
TTCCAAGTAACAACTATCATTTAAATAATTCATAAATCTTACTTTGAGTGGAAAGTTGAA
CTACATGGTCTCTGATACCCCTTCCATGCTCAATCTTTACAATCATACCTCATACATACA
AATAATTTCTACACACTTGTATTAGAAATGGGCCTCTTATAAAATGGAAGTTTTACCTAA
TAGAGTACATTTCCCTTGAACCCAATCCATATTAATTTTTCCCCCCTAAAACATCCACCC
TTAAAAGTTAACTGGACAATGCCGAACCCCGCTTCTGGGATCTTAGGATCCCTTAATATG
AGGGGAGGGATTAAACTTAGGTACGAACTCCCATCTGCAAAAAATTA
Restriction Sites NotI-NotI     
ACCN NM_003101
Insert Size 4000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003101.4, NP_003092.4
RefSeq Size 3407 bp
RefSeq ORF 1653 bp
Locus ID 6646
UniProt ID P35610
Domains MBOAT
Protein Families Transmembrane
Protein Pathways Steroid biosynthesis
Gene Summary The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]
Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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