Thymidine Kinase 1 (TK1) (NM_003258) Human Untagged Clone

CAT#: SC118084

TK1 (untagged)-Human thymidine kinase 1, soluble (TK1)



  "NM_003258" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal TK (Ab-13) antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms TK2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118084 sequence for NM_003258 edited (data generated by NextGen Sequencing)
ATGAGCTGCATTAACCTGCCCACTGTGCTGCCCGGCTCCCCCAGCAAGACCCGGGGGCAG
ATCCAGGTGATTCTCGGGCCGATGTTCTCAGGAAAAAGCACAGAGTTGATGAGACGCGTC
CGTCGCTTCCAGATTGCTCAGTACAAGTGCCTGGTGATCAAGTATGCCAAAGACACTCGC
TACAGCAGCAGCTTCTGCACACATGACCGGAACACCATGGAGGCGCTGCCCGCCTGCCTG
CTCCGAGACGTGGCCCAGGAGGCCCTGGGCGTGGCTGTCATAGGCATCGACGAGGGGCAG
TTTTTCCCTGACATCGTGGAGTTCTGCGAGGCCATGGCCAACGCCGGGAAGACCGTAATT
GTGGCTGCACTGGATGGGACCTTCCAGAGGAAGCCATTTGGGGCCATCCTGAACCTGGTG
CCGCTGGCCGAGAGCGTGGTGAAGCTGACGGCGGTGTGCATGGAGTGCTTCCGGGAAGCC
GCCTATACCAAGAGGCTCGGCACAGAGAAGGAGGTCGAGGTGATTGGGGGAGCAGACAAG
TACCACTCCGTGTGTCGGCTCTGCTACTTCAAGAAGGCCTCAGGCCAGCCTGCCGGGCCG
GACAACAAAGAGAACTGCCCAGTGCCAGGAAAGCCAGGGGAAGCCGTGGCTGCCAGGAAG
CTCTTTGCCCCACAGCAGATTCTGCAATGCAGCCCTGCCAACTGA

Clone variation with respect to NM_003258.4
33 t=>c;225 a=>g
>OriGene 5' read for NM_003258 unedited
CACACCACGGCCCACNACCCCCTTCCACCCGCCTTGTTCACATTTGTATACGACTCACTA
TAGGCGGCCGCGNATATCGCACGAGCGGGAGCGCAATGAGCTGCATTAACCTGCCCATGT
GCTGCCCGGCTCCCCCAGCAAGACCCGGGGGCAGATCCAGGTGATTCTCGGGCCGATGTT
CTCAGGAAAAAGCACAGAGTTGATGAGACGCGTCCGTCGCTTCCAGATTGCTCAGTACAA
GTGCCTGGTGATCAAGTATGCCAAAGACACTCGCTACAGCAGCAGCTTCTGCACACATGA
CCGGAACACCATGGAGGCGCTGCCCGCCTGCCTGCTCCGAGACGTGGCCCAGGAGGCCCT
GGGCGTGGCTGTCATAGGCATCGACGAGGGGCAGTTTTTCCCTGACATCGTGGAGTTCTG
CGAGGCCATGGCCAACGCCGGGAAGACCGTAATTGTGGCTGCACTGGATGGGACCTTCCA
GAGGAAGCCATTTGGGGCCATCCTGAACCTGGTGCCGCTGGCCGAGAGCGTGGTGAAGCT
GACGGCGGTGTGCATGGAGTGCTTCCGGGAAGCCGCCTATACCAAGAGGCTCGGCACAGA
GAAGGAGGTCGAGGTGATTGNGGGAGCAGACAAGTACCACTCCGTGTGTCGGCTCTGCTA
CTTCAAGAAAGCCTCAGGCCAGCCTGCCGGGCCGGACAACANAGAAACTGCCCAGTGCCA
GGAAAGCCAGGGGAAGCCGTGGCTGCCAGAAAGCTCTTTGCCCCACAGCAGATTCTGCAA
TGCAGCCCTGTCCACTGAGGGACCTGCGATGGCCGNCCGCTCCCTTCCTGCCACTGGCGN
CTACTGGACCCTGCCCTGCATGCTTGCCAGCCACTCCCAGAAGAAGTCGGGAAGG
>OriGene 3' read for NM_003258 unedited
GGGTTGCTATGGAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCCCCCC
GGGAATTTTCAATTATTTAATTTCAAAACCTACAGCAAAGGGGGGGCCCCGGCCCTTCCC
CCCTTAAAAAAAAACCCCCGGGGATCCAATTCCCAAAAGGGTGGGGCCCCCCCATTTTGG
GAAAAAATGCTTTTTTTCCTGGGGAAACCCTCCCCAAAGGTGGGAAGGGTTTTGACCCCA
AAAGGAAACAAAAGGGGGGGAACCCCGCCCCCCCCCGGGGGGGGGGGGGACCCCCTCCCA
GGCAAAAGGGCCTAACCCTCATGGGGGGCCAAGGCTAAAAGGGGCTGGCCCAGGCTTCCC
AGGGAAAAAAAAAACCCCCCTTTGAAACCCCCAAAGGGGAAAAACCAGGCTGATTTCAAC
AGGGGGGGGCTTTTCCCCCCACCCCAAGGAGAGGGAGGGGGCCAAATCCCAGGCCACCAA
GGGGGGCCGGTTCCACCCGGGGCAATCTTCCCCCCCCCTGAAAGGGAACCTTTAAACAAA
CCATTGGGTAAAAAAAAAAGGAACTTGCCCCGGGGGGGGCCCCCCCACAAGAGAAATTCT
CCAAAGGCCAAGGTGTGGGTATCTTCCCGCCTCCCAATTTTTTTTGGAAGGGCTGGGCAC
ATGCAGGGGAACCTCCCATTAGCCGCAATTGCAAAAAGAGACGGGGCCCCCCTCGAAGGT
CCCTTATTTGGGAGGGCTCCTTTTAAAAAATTGGGTGGGGGAAAAAAATTTCTGGGGGCC
CCGGGTTTCCCTGGTTTTTTGGGCGGGGGGAAGTTTTTTTTTTTTTCCGCCCCGAAGGTG
GGCTTAAG
Restriction Sites NotI-NotI     
ACCN NM_003258
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003258.1, NP_003249.1
RefSeq Size 1421 bp
RefSeq ORF 705 bp
Locus ID 7083
UniProt ID P04183
Domains TK
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
Gene Summary The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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