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UBE2D3 (NM_003340) Human Untagged Clone
CAT＃: SC118047
UBE2D3 (untagged)-Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1
CNY 1,200.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E2(17)KB3; UBC4/5; UBCH5C |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118047 sequence for NM_003340 edited (data generated by NextGen Sequencing)
ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCAGCACAA TGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATGGGACCT AATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACAGACTAC CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT AATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACAATTTCT AAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCCCTAGTG CCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCTCGGGAA TGGACTCAGAAGTATGCCATGTGA Clone variation with respect to NM_003340.5 >OriGene 5' read for NM_003340 unedited
GTAATACGACTTTCTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTAGTAAGATTGTGC TGTTCCGAGAAGGAGAAGGAAAAGGGCTTGACACGTATTCACTCGGCCCCGGACGTGGGA AGCAAGCCGTCTGGCTTCGGCCTCACATCGGTCCTGTGCTCGGGACGGCGGCGTTGGCGG ACTGATCCGCGGCGGTGAAGAGGCGCCTGTGTCTGGCAGAGCTGGTGTGAGACGAGACAA TCCTGCCCCGCCGCCGGGATAATCAAGAGTTTTGGCCGGACCTTTGAGCATACACCGAGA GAGTGAGGAGCCAGACGACAAGCACACACTATGGCGCTGAAACGGATTAATAAGGAACTT AGTGATTTGGCCCGTGACCCTCCAGCACAATGTTCTGCAGGTCCAGTTGGGGATGATATG TTTCATTGGCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGGCGGTGTATTC TTTTTGACAATTCATTTTCCTACAGACTACCCCTTCAAACCACCTAAGGTTGCATTTACA ACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTCGATATTCTAAGA TCACAGTGGTCGCCTGCTNTAACAATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTA TGTGATCCAAACCCAGATGACCCCCTAGTGCCAGAGATTGCACGGATCTATTAAACAGAC AGAGATAAGTACCACAGAATATCTCGGGAATGGACTCANNAGTATGCCATGTGATGCTAC CTTAAGTCAGNAATACCTGCATATAGCTGGNATAAACTTTAATACTGGTCCTTTNNTGAT TNTCTATCCGGCTGCTCCCTATCAGACTCATCTTTNTAAATNTATTTTTGGTTACCTCCT CCATTCATCCATGCTATTGC >OriGene 3' read for NM_003340 unedited
CCTTCAGCTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAT AGCATTGACTTTTATTCATTCATGTTAAGTCACCTGAAATGATATCTGTTGCATTTAAAT GCTCATTAATGTAAATGGCTGAACAAATCCATTTCAATGGATACTCAATATCCCACATAG ATAAGTCACTTCAACACAGCCTCATGACAACTCCCACACCAAAACAGTACTTTATTTTGC AAATTTTGACCCATTATTACTCCCATGTTGTTTTTTTTCAGCTGTCAAGTGTACAAGGTG AAGAGAAATTTCAGTTAGACAGGGGCTGCACCTGGAAAATAAATTTCTTAAACTCCATAA ACACATTTCAAAACTGCTGGGTCCCAAAAGTCCATCTATGAACTCTAGGGCTGCCAGTAT CATGTGCAGCACATTAAAGCTACACTATCCAAATAGCTTATTAATTCTGCATAAATCAGG TCAAGCAATGTGTCAACCTGTAACAGACTGTGCACTTTAACTGAACATGGCAAAATATTC ACTCTTTCGAACTTTACATCATCATTTGATGTCAAACAATGAAGCAAATCCCAACAGTAT ATACAAAAAACAGCTTTGCATTTACAAAAGATTGTTTCCCATACTGATTAATCCCAGCAG CTAACTCATTGGTTTATGCAACTTTATTGAAGAAAAATAAATCAATTACTAGCAGAGCTA TTAGTTGATCACTCATCCATTGACAACTTGCATCATTTATTCAGTGCTATATTAAACCAG TGTATTGGAAAGAAATATTAACTAATAGCCTCCAAGCCTCCTAAACATTTTAAATGGAAA ATTCCAAATTGTTTGGGAACCTTATTCTGGGATACAAAAGGGTGTTTGACCTCCAATTTC CATCCTTCTGAAAAACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003340 |
| Insert Size | 2090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003340.4, NP_003331.1 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 444 bp |
| Locus ID | 7323 |
| UniProt ID | P61077 |
| Domains | UBCc |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
UBE2L3 Polymorphism Amplifies NF-?B Activation and Promotes Plasma Cell Development, Linking Linear Ubiquitination to Multiple Autoimmune Diseases
,Lewis, MJ;Vyse, S;Shields, AM;Boeltz, S;Gordon, PA;Spector, TD;Lehner, PJ;Walczak, H;Vyse, TJ;,
Am. J. Hum. Genet.
,PubMed ID 25640675
[UBE2D3]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207371 | UBE2D3 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1 |
CNY 1,200.00 |
|
| RC207371L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1, Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC207371L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207371L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207371L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1, mGFP tagged |
CNY 3,600.00 |
|
| RG207371 | UBE2D3 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1 |
CNY 2,800.00 |
