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USP5 (NM_003481) Human Untagged Clone
CAT＃: SC117962
USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2
CNY 6,120.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ISOT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003481 edited
GGGCGGCCGCGAATTCGGCACGAGGGAGAAGCTGCTGCCGGTGTCATGGCGGAGCTGAGT GAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACCGGGTC CACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATC TGTATGAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACC GGCCAGCGAGTCTACTTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCT ACAGGCACTGGAGACCCACCCCGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGC GGGTTTGACCTTAGCGAGGAGAAGTTTGAATTAGACGAGGATGTGAAGATTGTCATTTTG CCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGACTGCCTGACATTGTCAGAGAT CGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGCAGGAG GTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAG TTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGA GAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGAT GGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCT GTCAAGCTGGGCACCATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGAC ATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAG ATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGATTGGT GAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTAC ACAGGCATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTC AGCATCCCTGACTTCCAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCC CCGACGGACCCTACCCAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTC TCCGGGGAGTATTCCAAGCCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAG AAGGAAGTTCAAGATGGCATTGCCCCTCGGATGTTCAAGGCCCTCATCGGCAAGGGCCAC CCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTATCAAC ATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTG GAGGAAAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTAC ATCATGCAGCTGCCTGTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTAC GAGGAGAAGAAGCGGCAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCC CAGGTGCCCTTCAGCTCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTC TGGAGCACGGCCCTGCAGGCCAAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTC CCTGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGCCCAAG AAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACA GGGCTGCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGAT GAGCCCAAAGCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTC CCTATGGACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCC ATGAACTGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCT GGCTCTAGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTG ACCACCATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACG AACAATAGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCT GAAGCTGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTG CCAGTGGGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGT CACATGGGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGA TGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGC TACATCTACTTCTACCAGAGAGTGGCCAGCTAAGAGCCTGCCTCACCCCTTACCAATGAG GGCAGGGGAAGACCACCTGGCATGAGGGAGAGGGGCTGAGGGATGGACTTCAGCCCCTCT GCTCTGTACCCTTTTTCCTTTTGTCCCCGGCAGCAGGGAAGAAGCTGGAGGCCGTGGGAG AATGGCTGGGCAGAGCAGAGGGGCAGCGATAGACTCTGGGGATGGAGCAGGACGGGGACG GGAGGGGCCGGCCACCTGTCTGTAAGGAGACTTTGTTGCTTCCCCTGCCCCCGGAATCCA CAGTGCTCTGCTTCTCTGTGTCGCCCCGCCCAGCCCCCTGGTGTGGAGGGAGGGGTCTCG TTTGTGCGCGTGGGTGTAGCTTTGTGCATCCTCTCCCAGTGGAGCGATCACCTGTGCCTC CCCTCCCCCTTTGTTTGCCCCTGTGTGGTTGGTCAAGGAGGGATGTGAGGGAAATAGGGA CCCCCCGACTTGCCCTCCTGCCTCAGTCTTTCCCCCACCCTGTCTCTTCCTTGTCCTTCT CTGGAAAATGCCAAAATACACGATGTGAATAAAAGTACAACGGCTAAAAAAAAAAAAAAA AAAC >OriGene 5' read for NM_003481 unedited
ATATTTGTATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGNAGAAGCTGCTGC CGGTGTCATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGT CCCTAAGGCTGGAGACCGGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGA GTCTGAGGGGGGCCTCTACATCTGTATGAACACGTTTCTGGGCTTTGGGAAACAGTATGT GGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTACTTGCACCTCCGGCGGACCCGGCG CCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCCCGGAAGAAGCCCACGCG GCTGGCTATTGGTGTTGAAGGCGGGTTTGACCTTAGCGAGGAGAAGTTTGAATTAGACGA GGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGG ACTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGA CTCAGCCTCCCGCAAGCAGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAA GCATGCCTTCAGCCTCAAGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGNGCTGGA AGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCC TCTGTGGGCGACGCTACTTCGATGGCAGTGGNGGCAACACCACGCTGTGGAGCACTACCG AGAGACGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGGAGCTGACGT GTACTNCATATGATGAGGATTGACATGGGTCCCTGGACNCCCAGCCTGGNCTGAGCACCC TGTCCACTTNNCGCATCGACATGGCTGAAGAATGCNNAAGACAGACAAGAACGATGACTG AGNTGGNAGAAGACATGACCAGCNGNATNNNGTGATGGNGNAGCTGACN >OriGene 3' read for NM_003481 unedited
CTATGGACCGCGCGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTAGCCGTTGTA CTTTTATTCACATCGTGTATTTTGGCATTTTCCAGAGAAGGACAAGGAAGAGACAGGGTG GGGGAAAGACTGAGGCAGGAGGGCAAGTCGGGGGGTCCCTATTTCCCTCACATCCCTCCT TGACCAACCACACAGGGGCAAACAAAGGGGGAGGGGAGGCACAGGTGATCGCTCCACTGG GAGAGGATGCACAAAGCTACACCCACGCGCACAAACGAGACCCCTCCCTCCACACCAGGG GGCTGGGCGGGGCGACACAGAGAAGCAGAGCACTGTGGATTCCGGGGGCAGGGGAAGCAA CAAAGTCTCCTTACAGACAGGTGGCCGGCCCCTCCCGTCCCCGTCCTGCTCCATCCCCAG AGTCTATCGCTGCCCCTCTGCTCTGCCCAGCCATTCTCCCACGGCCTCCAGCTTCTTCCC TGCTGCCGGGGACAAAAGGAAAAAGGGTACAGAGCAGAGGGGCTGAAGTCCATCCCTCAG CCCCTCTCCCTCATGCCAGGTGGTCTTCCCCTGCCCTCATTGGTAAGGGGTGAGGCAGGC TCTTAGCTGGCCACTCTCTGGTAGAAGTAGATGTAGCCCAGGTCCTTGGGCGGCTTCTCG GAGGCACACACTTTCTGGTCATTGTAGATCACCCATCTGCCTTCTTTCTTGATGTGGCAG ACGTAGTGACCACACATGGTAGAGGTGCCCATGTGACTAATGAAGGCAAAGAGCTGATAC TTTNCAGGACCATCCCGGACTTTANGTCCCACTGGCACAGACTCANAGATGGAGTCGGCA GCTGAGCGGCCTCTGAGATGTCATGCAGCTTCACATCCAGTCGTCATGTGACTGAAGATC ATCCCAGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003481 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003481.1, NP_003472.1 |
| RefSeq Size | 3102 bp |
| RefSeq ORF | 2508 bp |
| Locus ID | 8078 |
| UniProt ID | P45974 |
| Domains | UBA, UCH, zf-UBP |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202624 | USP5 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2 |
CNY 3,990.00 |
|
| RC202624L1 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2, Myc-DDK-tagged |
CNY 8,520.00 |
|
| RC202624L2 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC202624L3 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202624L4 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG202624 | USP5 (tGFP-tagged) - Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2 |
CNY 7,720.00 |
