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PPM1D (NM_003620) Human Untagged Clone
CAT＃: SC117895
PPM1D (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D)
CNY 4,512.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IDDGIP; JDVS; PP2C-DELTA; WIP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003620, the custom clone sequence may differ by one or more nucleotides
ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGACG TTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCGCCGCGGCGGTCGCTGTCTCA GCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGCGGCGAAGTCTCGGGGAAAGGCCCAGCGGTG GCAGCCCGAGAGGCTCGCGACCCTCTCCCGGACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCC GCCGTTCCTCCGTGGCCTTTTTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCG GGAGCACTTGTGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGGCCAAAGACTA TGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATTCGGGGCATGAAGATGTATGT AGCTCACGTAGGTGACTCAGGGGTGGTTCTTGGAATTCAGGATGACCCGAAGGATGACTTTGTCAGAGCT GTGGAGGTGACACAGGACCATAAGCCAGAACTTCCCAAGGAAAGAGAACGAATCGAAGGACTTGGTGGGA GTGTAATGAACAAGTCTGGGGTGAATCGTGTAGTTTGGAAACGACCTCGACTCACTCACAATGGACCTGT TAGAAGGAGCACAGTTATTGACCAGATTCCTTTTCTGGCAGTAGCAAGAGCACTTGGTGATTTGTGGAGC TATGATTTCTTCAGTGGTGAATTTGTGGTGTCACCTGAACCAGACACAAGTGTCCACACTCTTGACCCTC AGAAGCACAAGTATATTATATTGGGGAGTGATGGACTTTGGAATATGATTCCACCACAAGATGCCATCTC AATGTGCCAGGACCAAGAGGAGAAAAAATACCTGATGGGTGAGCATGGACAATCTTGTGCCAAAATGCTT GTGAATCGAGCATTGGGCCGCTGGAGGCAGCGTATGCTCCGAGCAGATAACACTAGTGCCATAGTAATCT GCATCTCTCCAGAAGTGGACAATCAGGGAAACTTTACCAATGAAGATGAGTTATACCTGAACCTGACTGA CAGCCCTTCCTATAATAGTCAAGAAACCTGTGTGATGACTCCTTCCCCATGTTCTACACCACCAGTCAAG TCACTGGAGGAGGATCCATGGCCAAGGGTGAATTCTAAGGACCATATACCTGCCCTGGTTCGTAGCAATG CCTTCTCAGAGAATTTTTTAGAGGTTTCAGCTGAGATAGCTCGAGAGAATGTCCAAGGTGTAGTCATACC CTCAAAAGATCCAGAACCACTTGAAGAAAATTGCGCTAAAGCCCTGACTTTAAGGATACATGATTCTTTG AATAATAGCCTTCCAATTGGCCTTGTGCCTACTAATTCAACAAACACTGTCATGGACCAAAAAAATTTGA AGATGTCAACTCCTGGCCAAATGAAAGCCCAAGAAATTGAAAGAACCCCTCCAACAAACTTTAAAAGGAC ATTAGAAGAGTCCAATTCTGGCCCCCTGATGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGT GCTCAGCCTGCAAGTCTCCCCACAACCTCACAGCGAAAGAACTCTGTTAAACTCACCATGCGACGCAGAC TTAGGGGCCAGAAGAAAATTGGAAATCCTTTACTTCATCAACACAGGAAAACTGTTTGTGTTTGCTGA >OriGene 5' read for NM_003620 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTCTCCGGGTCCGCC CCCTCCCCCTTCTCGGCGTCGTCGAAGATAAACAATAGTTGGCCGGCGAGCGCCTAGTGT GTCTCCCGCCGCCGGATTCGGCGGGCTGCGTGGGACCGGCGGGATCCCGGCCAGCCGGCC ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTAC ATGGAGGACGTTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCG CCGCGGCGGTCGCTGTCTCAGCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGC GGCGAAGTCTCGGGGAAAGGCCCAGCGGTGGCAGCCCGAGAGGCTCGCGACCCTCTCCCG GACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCCGCCGTTCCTCCGTGGCCTTT TTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTG TGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGG CCAAAGACTATGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATT CGGNGCATGAAGATGTATGTAGCTCACGTAGGTGACTCAGGGNGTGGCTTCTTGGNATTC AGGATGACCCGAAGGATGACTNTGTCAGAGCTGTGGGAGTGACACAGGACCCATAGCCAG AACTTCCCAGGNNAAGAGACGAATCGATGACTTGGTGGGAGTGTAAGAACAGTCCTGGGT GAATCGCGGTATTTGGGAACCGACTCGACTACTCACATGGACCTGNTAGAAGAGCCAGTT ATGACAAC >OriGene 3' read for NM_003620 unedited
ACCGCGGCCGCAATTTAGAGTCGAGCCCTTTTCTTTTTTTTTTTCTTTTTCTTTTTTTTT TTTTTTTTTTTTTTTCTTTTTGGGGGACCGGTCACTGGTTTATTAAAAGAACCTTTTCCC CTATAATCAATGAAAACTGTTTGATCTAAAAAAATTTAAAAAATGGCGGGAATTAACCGC CCCCTTTGTTTTTGAAATAACAAGGACAATTTCCCCTTTTTTAACCCAACCCGAAAAGGG GGTAATTCCTTTAAAACCTAAATATGCCATTGGTCTGGGATGAAAAAAAAGGGAATTGCC CTTAAACTGTTTTAAGGCAACACCCTTCCTTTTATGCCGAAAACTTAATAAGCCCTTTCG CAGACCCAGGCTTTAAAAAAACGCCCCTGGTGGAAAACACACCGGCTAGCCCCTTAATTG CGCCAACACCAACTGGTCTCGGCGTTCATCCTTCTATGAAACATTTACCTCCTAATTCCG CCATTCCGAAATCAAAACTTTTGTTTAACCGACCCCTTGGCTTCTTCAAAACATTAAGCG ACACCCCCGCCGCCCTGAAAATACACCACTTTTCCCCCCCGCCTAATAATTTACCTTACA AAGTCGTACCGCTTCCAACACAACGCTCCATACAAATTCTCTACCTACCCCTCTCACACG TCTACCCCGTTTTTCAAAACCCAACAAAATCTTTGGATTTCTCCCAACCCCCATCCCTTT TGTACCCCCTCCTCGTATTACACACCTTTAACTCCGGTCCTTCCACACTGAATTTCGCGC CTTCCCACTCCCCCATTTCTCGCGTTCTTTCCTTCACCCTTTAACCCCTTTCCCTTTACC CCCCCCCGCCCGATAAACCGCCCATCCAAAAGAAACATGTTCTCCTTTATCCCCCCCTCA AAAAGGTACACTGCCCCACTTAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003620 |
| Insert Size | 2840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003620.2, NP_003611.1 |
| RefSeq Size | 3163 bp |
| RefSeq ORF | 1818 bp |
| Locus ID | 8493 |
| UniProt ID | O15297 |
| Domains | PP2C |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | p53 signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209328 | PPM1D (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D) |
CNY 6,752.00 |
|
| RC209328L1 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D), Myc-DDK-tagged |
CNY 9,152.00 |
|
| RC209328L2 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D), mGFP tagged |
CNY 9,152.00 |
|
| RC209328L3 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D), Myc-DDK-tagged |
CNY 9,152.00 |
|
| RC209328L4 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D), mGFP tagged |
CNY 7,980.00 |
|
| RG209328 | PPM1D (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D) |
CNY 6,650.00 |
