PPM1D (NM_003620) Human Untagged Clone

CAT#: SC117895

PPM1D (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D)



  "NM_003620" in other vectors (6)

CNY 4,512.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
PPM1D mouse monoclonal antibody,clone OTI3E1
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms IDDGIP; JDVS; PP2C-DELTA; WIP1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003620, the custom clone sequence may differ by one or more nucleotides


ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGACG
TTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCGCCGCGGCGGTCGCTGTCTCA
GCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGCGGCGAAGTCTCGGGGAAAGGCCCAGCGGTG
GCAGCCCGAGAGGCTCGCGACCCTCTCCCGGACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCC
GCCGTTCCTCCGTGGCCTTTTTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCG
GGAGCACTTGTGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT
GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGGCCAAAGACTA
TGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATTCGGGGCATGAAGATGTATGT
AGCTCACGTAGGTGACTCAGGGGTGGTTCTTGGAATTCAGGATGACCCGAAGGATGACTTTGTCAGAGCT
GTGGAGGTGACACAGGACCATAAGCCAGAACTTCCCAAGGAAAGAGAACGAATCGAAGGACTTGGTGGGA
GTGTAATGAACAAGTCTGGGGTGAATCGTGTAGTTTGGAAACGACCTCGACTCACTCACAATGGACCTGT
TAGAAGGAGCACAGTTATTGACCAGATTCCTTTTCTGGCAGTAGCAAGAGCACTTGGTGATTTGTGGAGC
TATGATTTCTTCAGTGGTGAATTTGTGGTGTCACCTGAACCAGACACAAGTGTCCACACTCTTGACCCTC
AGAAGCACAAGTATATTATATTGGGGAGTGATGGACTTTGGAATATGATTCCACCACAAGATGCCATCTC
AATGTGCCAGGACCAAGAGGAGAAAAAATACCTGATGGGTGAGCATGGACAATCTTGTGCCAAAATGCTT
GTGAATCGAGCATTGGGCCGCTGGAGGCAGCGTATGCTCCGAGCAGATAACACTAGTGCCATAGTAATCT
GCATCTCTCCAGAAGTGGACAATCAGGGAAACTTTACCAATGAAGATGAGTTATACCTGAACCTGACTGA
CAGCCCTTCCTATAATAGTCAAGAAACCTGTGTGATGACTCCTTCCCCATGTTCTACACCACCAGTCAAG
TCACTGGAGGAGGATCCATGGCCAAGGGTGAATTCTAAGGACCATATACCTGCCCTGGTTCGTAGCAATG
CCTTCTCAGAGAATTTTTTAGAGGTTTCAGCTGAGATAGCTCGAGAGAATGTCCAAGGTGTAGTCATACC
CTCAAAAGATCCAGAACCACTTGAAGAAAATTGCGCTAAAGCCCTGACTTTAAGGATACATGATTCTTTG
AATAATAGCCTTCCAATTGGCCTTGTGCCTACTAATTCAACAAACACTGTCATGGACCAAAAAAATTTGA
AGATGTCAACTCCTGGCCAAATGAAAGCCCAAGAAATTGAAAGAACCCCTCCAACAAACTTTAAAAGGAC
ATTAGAAGAGTCCAATTCTGGCCCCCTGATGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGT
GCTCAGCCTGCAAGTCTCCCCACAACCTCACAGCGAAAGAACTCTGTTAAACTCACCATGCGACGCAGAC
TTAGGGGCCAGAAGAAAATTGGAAATCCTTTACTTCATCAACACAGGAAAACTGTTTGTGTTTGCTGA


>OriGene 5' read for NM_003620 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTCTCCGGGTCCGCC
CCCTCCCCCTTCTCGGCGTCGTCGAAGATAAACAATAGTTGGCCGGCGAGCGCCTAGTGT
GTCTCCCGCCGCCGGATTCGGCGGGCTGCGTGGGACCGGCGGGATCCCGGCCAGCCGGCC
ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTAC
ATGGAGGACGTTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCG
CCGCGGCGGTCGCTGTCTCAGCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGC
GGCGAAGTCTCGGGGAAAGGCCCAGCGGTGGCAGCCCGAGAGGCTCGCGACCCTCTCCCG
GACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCCGCCGTTCCTCCGTGGCCTTT
TTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTG
TGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT
GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGG
CCAAAGACTATGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATT
CGGNGCATGAAGATGTATGTAGCTCACGTAGGTGACTCAGGGNGTGGCTTCTTGGNATTC
AGGATGACCCGAAGGATGACTNTGTCAGAGCTGTGGGAGTGACACAGGACCCATAGCCAG
AACTTCCCAGGNNAAGAGACGAATCGATGACTTGGTGGGAGTGTAAGAACAGTCCTGGGT
GAATCGCGGTATTTGGGAACCGACTCGACTACTCACATGGACCTGNTAGAAGAGCCAGTT
ATGACAAC
>OriGene 3' read for NM_003620 unedited
ACCGCGGCCGCAATTTAGAGTCGAGCCCTTTTCTTTTTTTTTTTCTTTTTCTTTTTTTTT
TTTTTTTTTTTTTTTCTTTTTGGGGGACCGGTCACTGGTTTATTAAAAGAACCTTTTCCC
CTATAATCAATGAAAACTGTTTGATCTAAAAAAATTTAAAAAATGGCGGGAATTAACCGC
CCCCTTTGTTTTTGAAATAACAAGGACAATTTCCCCTTTTTTAACCCAACCCGAAAAGGG
GGTAATTCCTTTAAAACCTAAATATGCCATTGGTCTGGGATGAAAAAAAAGGGAATTGCC
CTTAAACTGTTTTAAGGCAACACCCTTCCTTTTATGCCGAAAACTTAATAAGCCCTTTCG
CAGACCCAGGCTTTAAAAAAACGCCCCTGGTGGAAAACACACCGGCTAGCCCCTTAATTG
CGCCAACACCAACTGGTCTCGGCGTTCATCCTTCTATGAAACATTTACCTCCTAATTCCG
CCATTCCGAAATCAAAACTTTTGTTTAACCGACCCCTTGGCTTCTTCAAAACATTAAGCG
ACACCCCCGCCGCCCTGAAAATACACCACTTTTCCCCCCCGCCTAATAATTTACCTTACA
AAGTCGTACCGCTTCCAACACAACGCTCCATACAAATTCTCTACCTACCCCTCTCACACG
TCTACCCCGTTTTTCAAAACCCAACAAAATCTTTGGATTTCTCCCAACCCCCATCCCTTT
TGTACCCCCTCCTCGTATTACACACCTTTAACTCCGGTCCTTCCACACTGAATTTCGCGC
CTTCCCACTCCCCCATTTCTCGCGTTCTTTCCTTCACCCTTTAACCCCTTTCCCTTTACC
CCCCCCCGCCCGATAAACCGCCCATCCAAAAGAAACATGTTCTCCTTTATCCCCCCCTCA
AAAAGGTACACTGCCCCACTTAAG
Restriction Sites NotI-NotI     
ACCN NM_003620
Insert Size 2840 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003620.2, NP_003611.1
RefSeq Size 3163 bp
RefSeq ORF 1818 bp
Locus ID 8493
UniProt ID O15297
Domains PP2C
Protein Families Druggable Genome, Phosphatase
Protein Pathways p53 signaling pathway
Gene Summary The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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