eIF4GII (EIF4G3) (NM_003760) Human Untagged Clone
CAT#: SC117780
EIF4G3 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3
CNY 10,856.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | eIF-4G 3; eIF4G 3; eIF4GII |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC117780 sequence for NM_003760 edited (data generated by NextGen Sequencing)
ATGAATTCACAACCTCAAACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCT AGAGCTACCATCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCA GTGTACCAGGCTAATCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTG CCCCAGGGGCCTCAGTACTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTTGGG CCCCCCCAACAATATCCAGTTCAACCACCGGGGCCAGGTCCTTTTTATCCTGGACCAGGA CCTGGGGACTTCCCCAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCCGGTGTATCAG TCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCTCCAGCCAAGAGAGAGAAAAAA ACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGGAGATTATGTCT GGAGGTGGCAGCAGAAATCCTACTCCACCCATAGGAAGACCCACGTCCACACCTACTCCT CCTCAGCAGCTGCCCAGCCAGGTCCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAG AGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAGGAG AAGCCAAAACCAGATCCAGTGTTAAAGTCTCCTTCCCCAGTCCTTAGGCTAGTCCTCAGT GGAGAGAAGAAAGAACAAGAAGGCCAGACATCTGAAACTACTGCAATAGTATCCATAGCA GAGCTTCCTCTGCCTCCATCACCTACCACTGTTTCTTCTGTTGCTCGAAGTACAATTGCA GCCCCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAGATGACAGA TGTGAACTCTCATCCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACA GAAACATCAGACCCTTTACCAACAAATGAAAATGATGATGATATATGCAAGAAACCCTGT AGTGTAGCACCTAATGATATTCCACTGGTTTCTAGTACTAACCTAATTAATGAAATAAAT GGAGTTAGCGAAAAATTATCAGCCACGGAGAGCATTGTGGAAATAGTAAAACAGGAAGTA TTGCCATTGACTCTTGAATTGGAGATTCTCGAAAATCCCCCAGAAGAAATGAAACTGGAG TGTATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTTCCTCCAACTCCTCCAACT CCTCCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTACTGTTAGT TCTCCGAGTGCTGCCATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACT TGCCTTAGTGAAGATGCAAAAGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAA ACAGAAGAGATTTTGGATTCTCAAAACTTAAATTCAAGAAGGAGCCCTGTCCCAGCTCAA ATAGCTATAACTGTACCAAAGACATGGAAGAAACCAAAAGATCGGACCCGAACCACTGAA GAGATGTTAGAGGCAGAATTGGAGCTTAAAGCTGAAGAGGAGCTTTCCATTGACAAAGTA CTTGAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCTGAAAGAGACCCCTCTGAC CTAAAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTACGTAATGGT GCTGAGAGTGTTTCTGAGGGTGAAGGAATAGATGCTAATTCAGGCTCCACAGATAGTTCT GGTGATGGGGTTACATTTCCATTTAAACCAGAATCCTGGAAGCCTACTGATACTGAAGGT AAGAAGCAGTATGACAGGGAGTTTCTGCTGGACTTCCAGTTCATGCCTGCCTGTATACAA AAACCAGAGGGCCTGCCTCCTATCAGTGATGTGGTTCTTGACAAGATCAACCAACCCAAA TTGCCAATGCGAACTCTGGATCCTCGAATTTTGCCTCGAGGACCAGACTTTACACCAGCC TTTGCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTACCTTTGTTGAATGTTGGG TCACGAAGATCTCAACCTGGCCAAAGAAGAGAACCCAGAAAGATCATCACAGTTTCTGTA AAAGAAGATGTACACCTGAAAAAGGCAGAAAATGCCTGGAAGCCAAGCCAAAAACGAGAC AGCCAAGCCGATGATCCCGAAAACATTAAAACCCAGGAGCTTTTTAGAAAAGTTCGAAGT ATCTTAAATAAATTGACACCACAGATGTTCAATCAACTGATGAAGCAAGTGTCAGGACTT ACTGTTGACACAGAGGAGCGGCTGAAAGGAGTTATTGACCTGGTCTTTGAGAAGGCTATT GATGAACCCAGTTTCTCTGTGGCTTACGCAAACATGTGTCGATGTCTAGTAACGCTGAAA GTACCCATGGCAGACAAGCCTGGTAACACAGTGAATTTCCGGAAGCTGCTACTGAACCGT TGCCAGAAGGAGTTTGAAAAAGATAAAGCAGATGATGATGTCTTTGAGAAGAAGCAGAAA GAACTTGAGGCTGCCAGTGCTCCAGAGGAGAGGACAAGGCTTCATGATGAACTGGAAGAA GCCAAGGACAAAGCCCGGCGGAGATCCATTGGCAACATCAAGTTTATTGGAGAACTCTTT AAACTCAAAATGCTGACTGAAGCCATCATGCATGACTGTGTGGTGAAGCTGCTAAAGAAC CATGATGAAGAATCCCTGGAGTGCCTGTGTCGCCTGCTCACCACCATTGGCAAAGACTTG GACTTTGAAAAAGCAAAGCCACGTATGGACCAGTACTTTAATCAGATGGAGAAAATTGTG AAAGAAAGAAAAACCTCATCTAGGATTCGGTTCATGCTTCAAGATGTTATAGACCTAAGG CTGTGCAATTGGGTATCTCGAAGAGCAGATCAAGGGCCTAAAACTATCGAACAGATTCAC AAAGAGGCTAAAATAGAAGAACAAGAAGAGCAAAGGAAGGTCCAGCAACTCATGACCAAA GAGAAGAGAAGACCAGGTGTCCAGAGAGTGGACGAAGGTGGGTGGAACACTGTACAAGGG GCCAAGAACAGTCGGGTACTGGACCCCTCAAAATTCCTAAAAATCACTAAGCCTACAATT GATGAAAAAATTCAGCTGGTACCTAAAGCACAGCTAGGCAGCTGGGGAAAAGGCAGCAGT GGTGGAGCAAAGGCAAGTGAGACTGATGCCTTACGGTCAAGTGCTTCCAGTTTAAACAGA TTCTCTGCCCTGCAACCTCCAGCACCCTCAGGGTCCACGCCATCCACGCCTGTAGAGTTT GATTCCCGAAGGACCTTAACTAGTCGTGGAAGTATGGGCAGGGAGAAGAATGACAAGCCC CTTCCATCTGCAACAGCTCGGCCAAATACTTTCATGAGGGGTGGCAGCAGTAAAGACCTG CTAGACAATCAGTCTCAAGAAGAGCAGCGGAGAGAGATGCTGGAGACCGTGAAGCAGCTC ACAGGAGGTGTGGATGTGGAGAGGAACAGCACTGAGGCTGAGCGAAATAAAACAAGGGAG TCAGCAAAACCAGAAATTTCAGCAATGTCAGCTCATGACAAGGCTGCATTATCAGAAGAG GAACTGGAGAGGAAGTCGAAATCTATCATTGATGAATTTCTACACATTAATGATTTTAAG GAAGCCATGCAGTGTGTGGAAGAGCTGAATGCCCAGGGCCTACTACATGTTTTTGTGAGA GTGGGAGTGGAGTCCACCCTGGAAAGGAGCCAGATCACCAGGGATCACATGGGCCAACTA CTCTATCAGCTGGTACAGTCAGAAAAACTCAGCAAACAGGACTTTTTCAAAGGTTTTTCA GAAACTTTGGAATTGGCAGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTT GCTGAACTGGTGACCCCCATGTTAAAAGAAGGTGGAATCTCCATGAGAGAACTTACCATA GAATTTAGCAAACCTTTACTTCCTGTTGGAAGAGCTGGGGTCTTGCTATCTGAAATATTG CACCTACTATGCAAACAAATGAGCCATAAGAAAGTGGGAGCCTTATGGAGGGAGGCTGAC CTCAGCTGGAAGGACTTTTTACCAGAAGGAGAAGATGTACATAATTTTCTTTTGGAGCAG AAGTTGGACTTCATAGAGTCTGACAGTCCCTGTTCCTCTGAAGCACTTTCAAAGAAAGAA CTGTCTGCCGAAGAGCTGTATAAGCGACTCGAGAAACTCATTATTGAGGACAAAGCGAAT GATGAACAGATCTTTGACTGGGTAGAGGCTAATCTAGACGAAATCCAGATGAGTTCACCT ACATTCCTTAGAGCTTTAATGACTGCTGTTTGTAAAGCAGCTATTATAGCCGACTCTTCT ACCTTCAGAGTGGACACTGCTGTTATCAAGCAGAGAGTGCCGATCTTACTCAAGTACCTA GACTCAGATACAGAGAAGGAACTGCAAGCACTTTATGCACTACAAGCATCGATAGTAAAA CTTGATCAACCTGCCAATTTGCTGCGGATGTTTTTTGATTGTCTATATGACGAGGAGGTG ATCTCCGAGGATGCCTTCTACAAATGGGAGAGCAGCAAGGACCCTGCAGAGCAGAATGGG AAGGGCGTGGCTCTGAAATCTGTCACGGCATTCTTCACGTGGCTGCGGGAAGCAGAAGAG GAGTCTGAGGATAACTAA Clone variation with respect to NM_003760.4 3838 t=>c >OriGene 5' read for NM_003760 unedited
CCGCGTAATTCGGCACGAGGGTCCCCCCTCCNACCCGAACATCAGGGCCCTCCAGACTCA GGCGCCCCAACAAATTCCTAGAGGACCTGTGCAACAACCTCTTGAGGATCGAATCTTCAC TCCCGCTGTCTCAGCAGTCTACAGCACGGTAACACAAGTGGCAAGACAGCCGGGAACCCC TACCCCATCCCCTTATTCAGCACATGAAATAAACAAGGGGCATCCAAATCTTGCGGCAAC GCCCCCGGGACATGCATCGTCCCCTGGACTCTCTCAAACCCCTTATCCCTCTGGACAGAA TGCAGGTCCAACCACGCTGGTATACCCTCAAACCCCTCAGACAATGAATTCACAACCTCA AACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCTAGAGCTACCATCCCGAA CAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCAGTGTACCAGGCTAATCA GCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTGCCCCAGGGGCCTCAGTA CTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCCAACAATATCC AGTTCAACCACCGGGGCCAGGTCCTTTNTATCCTGGACCAGGACCTGGGGACTTCCCCAA TGCTTATGGAACGCCTTTTTACCCAGTCAGCCGGTGTATCAGTCAGCACCTATCATAGTG CCTACGCAGCAACAGCCGNCTTCAGCCAAGAGAGAGAAAAAACTATTAGAATTCGGGATC CAACCAGGGGAGTAAAGACAAACAGAGGAGATATGTCTGGAGTGGCAGCAGAATCCTACT CCACCATAGGA >OriGene 3' read for NM_003760 unedited
TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAACAATTCTT GGAGTTCTGTGGTCCACAGCATTTCCTTCTGTTTCAATGTTATGTATGTTTTGATTACTA TTGTGATTTTTTAAATTTTCTGAAGCAAGCTGAGAGGCAGGCAGAAAGATTTGATGCCAA AAAAAAAAAAAATCTTTCTTACCTTGTTCACCCCAAACTTTCTCAAATCTGGACTAAATG CTATACCTTAAAACAAACATGAGGTGCATCTTGAAGGGGAGGGAAATTTATTTCTCTGCT TTTCTATTATACAAGTTGTTTACAGAAACTGCAAATTAAAAAATTACACTGGCATTTGCA GTCCTTAAAATAAATTAAAAGTTCTCAACTTTTTTTTTTTTTGCTAAACATTTTTTTAAG TATGAGTCCTTGTTTAAAAAGAAAAGATTAAAACAGAAAATATTTTCTATAAATAATACA TGTATTTTGGTTTTAGTGCTCCCGCCCTAAGGTTTGAAGTTTACTTTTATCCAGTACCTT TTTCCTCCATGACCACCTTTTTTTCTCTTTCCCCTCTCCCACTCGTGCACACGTGGGGGT TTCTGCGAGAATTGGCCTTGCTGCACTGNGATTGGCGAAGACGTGAAACTTTCTAAAAAA AACTTAAACCGTTCCTTTTGTTTCTTTTGTGCATTTGAAGTTTTAGTTATCCTCAAACTC CTCTTCTGCTTCCCGAAGCCACGCGAAGAATGCCGTGACAGATTTCAAAAGCACGCCCTT CCCATTTTGTTTGCCAGGTCCCTGCTGCTCTCCCCATTGTAGACGCCTCCTTGGCAATCA CCTCCTGTGTATAAGACATTAAAAAAATTCGNACCAATTGGCGGGTGGACAAGCTTACTC TCGAGCTTGCACGGCACAAGGGCTGGACCCCTTCCTTGTATCCGTCCCAGGACCTGGACC AGATCGGCCTTTCTGCTGACACCCCACGGTCCCACGTCCGTCCG |
Restriction Sites | NotI-NotI |
ACCN | NM_003760 |
Insert Size | 5650 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003760.2, NP_003751.2 |
RefSeq Size | 5880 bp |
RefSeq ORF | 4758 bp |
Locus ID | 8672 |
UniProt ID | O43432 |
Domains | eIF5C, MIF4G, MA3 |
Protein Families | Transcription Factors |
Protein Pathways | Viral myocarditis |
Gene Summary | The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016] Transcript Variant: This variant (3) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (3) lacks an internal segment, as compared to isoform 1. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212591 | EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 10,848.00 |
|
RC212591L3 | Lenti-ORF clone of EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 17,670.00 |
|
RC212591L4 | Lenti-ORF clone of EIF4G3 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 17,670.00 |
|
RG212591 | EIF4G3 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 12,448.00 |
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